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The map label for this gene is mltB [H]

Identifier: 146338987

GI number: 146338987

Start: 2056928

End: 2058166

Strand: Reverse

Name: mltB [H]

Synonym: BRADO1935

Alternate gene names: 146338987

Gene position: 2058166-2056928 (Counterclockwise)

Preceding gene: 146338988

Following gene: 146338986

Centisome position: 27.6

GC content: 69.33

Gene sequence:

>1239_bases
ATGGCGCCGGAGCCCGCCGCTCCGCGACCGAAGCAGGCGCCGTCGATGCGCGCCGCGGCCTGCCATAACGGCCAGAATTT
CGATCGCTTTCTGGCCGAGCTGAAGCAACGCGCCGTCGCCGAGGGCGTGTCGCAGCGCGCGATCGCCGCCGCCTCGCCCT
ACCTCACTTACGACCAGGGCATCGTCAACCGCGACCGCGGCCAGCGCGTGTTCGGCCAGCTGTTCACCGAGTTCGCCGGC
CGCATGGCGGCGGTGTACCGGATGCAGAACGGCCAGCAGCACATCAAGGCGCATGCGGCGGCGTTCGCGCGGGCCGAGAA
GGAATATGGCGTGCCGCCGGCGGTGATCGCCGCGTTCTGGGGCCTGGAGAGCGATTTCGGCTCCAACATGGGCAATCTGC
CGGTGCTGCCGTCGCTGGTCTCGCTGGCCTATGACTGCCGCCGCAGCGAGCGCTTCCAGAACGAGACGATCGCCGCGCTG
AAGGTCATCGACCGCGGCGACCTCACGCCCACCGAGATGATCGGCTCCTGGGCCGGCGAGCTCGGCCAGACGCAATTCCT
GCCGACGCACTACGTCACCTACGGCGTCGACTACGACGGCGATGGCCGGCGCGACCTCTTGCGCAGCGCGCCCGACGTGA
TCGGCTCGACAGCCAACTACATCGCGACCGGATTGAAATGGCGTCGCGGCGAGCCGTGGCTGGAGGAGATCCGCGTGCCC
CAGACCTTCCCCTGGGAGCAGGCCGATCTCACCGTCAAAGCGCCACGCAGCAAATGGGCCGGGCTCGGCGTCACCTATGC
CGATGGCCGGCCGCTGCCGAACGATGCGCTGGAGGCCTCGGTGCTGCTGCCGATGGGGCGTAACGGCCCGGCGTTTTTGG
CCTACGCCAATTTCGCCGCCTACACCGAATGGAACAACTCGCTGATTTATTCGACCACCGCGGCCTATCTCGCCACCCGC
ATCGCCGGCGCCGCGCCGATGCGCAAAGCCAGCGGCCCCGTCGCTCAGCTGCCCTTCAACGAGATCAAGGAGCTGCAGCA
ATTGCTGGTGAAGGCCGGCTTCGACGTCGGCAAGGTCGACGGCGTGCTCGGCCAGCAGAGCCGCACGGCTGTCAAGACGA
TGCAGCTGCGGCTCGGGCTGCCGGCGGATTCCTGGCCGACCGCCGAGCTCCTCGCGCGGATGCGCGGCAACACCCCGCGC
GCGCAGGCCGCGCCGGCTGCGGCCACCGCCACGCGCTGA

Upstream 100 bases:

>100_bases
CGATCGGTGAGACCGGATGCGAACCTCTTCGACTGCCTTGCGCGTGACCTTCAGCGCGCTCGCAACCTCGTTCATGCTGG
TTGTCAGCGCGTCGGCGCAA

Downstream 100 bases:

>100_bases
TCCAACAACCGCCAGAGGACAACGGCCATGCCCCACGTCATCGTCAAGCTCTATCCCGGCAGGTCCGAGCAGCAGAAGCA
GGCGCTCGCGAAGGCCCTCA

Product: hypothetical protein

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MAPEPAAPRPKQAPSMRAAACHNGQNFDRFLAELKQRAVAEGVSQRAIAAASPYLTYDQGIVNRDRGQRVFGQLFTEFAG
RMAAVYRMQNGQQHIKAHAAAFARAEKEYGVPPAVIAAFWGLESDFGSNMGNLPVLPSLVSLAYDCRRSERFQNETIAAL
KVIDRGDLTPTEMIGSWAGELGQTQFLPTHYVTYGVDYDGDGRRDLLRSAPDVIGSTANYIATGLKWRRGEPWLEEIRVP
QTFPWEQADLTVKAPRSKWAGLGVTYADGRPLPNDALEASVLLPMGRNGPAFLAYANFAAYTEWNNSLIYSTTAAYLATR
IAGAAPMRKASGPVAQLPFNEIKELQQLLVKAGFDVGKVDGVLGQQSRTAVKTMQLRLGLPADSWPTAELLARMRGNTPR
AQAAPAAATATR

Sequences:

>Translated_412_residues
MAPEPAAPRPKQAPSMRAAACHNGQNFDRFLAELKQRAVAEGVSQRAIAAASPYLTYDQGIVNRDRGQRVFGQLFTEFAG
RMAAVYRMQNGQQHIKAHAAAFARAEKEYGVPPAVIAAFWGLESDFGSNMGNLPVLPSLVSLAYDCRRSERFQNETIAAL
KVIDRGDLTPTEMIGSWAGELGQTQFLPTHYVTYGVDYDGDGRRDLLRSAPDVIGSTANYIATGLKWRRGEPWLEEIRVP
QTFPWEQADLTVKAPRSKWAGLGVTYADGRPLPNDALEASVLLPMGRNGPAFLAYANFAAYTEWNNSLIYSTTAAYLATR
IAGAAPMRKASGPVAQLPFNEIKELQQLLVKAGFDVGKVDGVLGQQSRTAVKTMQLRLGLPADSWPTAELLARMRGNTPR
AQAAPAAATATR
>Mature_411_residues
APEPAAPRPKQAPSMRAAACHNGQNFDRFLAELKQRAVAEGVSQRAIAAASPYLTYDQGIVNRDRGQRVFGQLFTEFAGR
MAAVYRMQNGQQHIKAHAAAFARAEKEYGVPPAVIAAFWGLESDFGSNMGNLPVLPSLVSLAYDCRRSERFQNETIAALK
VIDRGDLTPTEMIGSWAGELGQTQFLPTHYVTYGVDYDGDGRRDLLRSAPDVIGSTANYIATGLKWRRGEPWLEEIRVPQ
TFPWEQADLTVKAPRSKWAGLGVTYADGRPLPNDALEASVLLPMGRNGPAFLAYANFAAYTEWNNSLIYSTTAAYLATRI
AGAAPMRKASGPVAQLPFNEIKELQQLLVKAGFDVGKVDGVLGQQSRTAVKTMQLRLGLPADSWPTAELLARMRGNTPRA
QAAPAAATATR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=236, Percent_Identity=30.0847457627119, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 44841; Mature: 44710

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
APEPAAPRPKQAPSMRAAACHNGQNFDRFLAELKQRAVAEGVSQRAIAAASPYLTYDQG
CCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCC
IVNRDRGQRVFGQLFTEFAGRMAAVYRMQNGQQHIKAHAAAFARAEKEYGVPPAVIAAFW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GLESDFGSNMGNLPVLPSLVSLAYDCRRSERFQNETIAALKVIDRGDLTPTEMIGSWAGE
CCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LGQTQFLPTHYVTYGVDYDGDGRRDLLRSAPDVIGSTANYIATGLKWRRGEPWLEEIRVP
CCCCCCCCCEEEEEECCCCCCCHHHHHHHCHHHHCCCHHHHHHCCCCCCCCCHHHHCCCC
QTFPWEQADLTVKAPRSKWAGLGVTYADGRPLPNDALEASVLLPMGRNGPAFLAYANFAA
CCCCCCCCCEEEECCCCCCCCCCEEECCCCCCCCHHHCCEEEEECCCCCCEEEEEECCCE
YTEWNNSLIYSTTAAYLATRIAGAAPMRKASGPVAQLPFNEIKELQQLLVKAGFDVGKVD
EEECCCCEEEHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
GVLGQQSRTAVKTMQLRLGLPADSWPTAELLARMRGNTPRAQAAPAAATATR
HHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]