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The map label for this gene is nemA [H]

Identifier: 146338979

GI number: 146338979

Start: 2048846

End: 2049943

Strand: Reverse

Name: nemA [H]

Synonym: BRADO1926

Alternate gene names: 146338979

Gene position: 2049943-2048846 (Counterclockwise)

Preceding gene: 146338984

Following gene: 146338977

Centisome position: 27.49

GC content: 68.12

Gene sequence:

>1098_bases
ATGAGCACATCGAAACTGTTCGAGCCCACCAAGCTCGGTCCGATCACCCTGGCCAATCGCGTCGTGATGGCACCGCTGAC
GCGCAACCGCGCCGTCGAGGGCCTCGCCCCGGGTCCGCTGACAGCCACCTACTACGCGCAGCGCGCCAGCGCCGGGCTCC
TGATCTCCGAGGCCAGCCAAGTGTCGCAGCAGGGCCAGGGCTATCAGGACACGCCCGGCATCTATTCCAAGGCGCAGATC
GCCGGCTGGCGCAAGGTCACCGACGCGGTGCACGCCAAGGGCGGCCGCATCTTCATCCAGCTCTGGCATGTCGGCCGCAT
CTCGCATGTCTCGTTGCAGCCCGATGGCGGCGCGCCGGTGGCGCCCTCGGCGATCGCCGCCAAGACCAAGACCTTCGTGG
GGGGCACCTTCGCCGATGTCTCGACGCCGCGCGCGCTCGAGCTCGCCGAGATCCCCGGCATCGTCGAGGCGTTCAAGCAG
GCCGCGCTGAATGCGATCGAGGCCGGCTTCGACGGCATCGAGATCCACGGCGCCAATGGCTATCTGCTCGACCAGTTCGC
CAAGGACGGCGCCAACAAGCGCACCGACGCCTATGGCGGCTCGATCGAGAACCGCGCCCGGCTGATGATCGAGGTCGCGA
AGGCCGTGTCCGGCGCTATTGGCGCCGACAGGACCGGCATCCGCCTGTCGCCGGTGACGCCGGCCAACGACGTCAGCGAT
TCCAATCCGCAGGCGCTGTTCGACTATATCGTCGATCAGCTCAACGCGCTGAAGCTCGTCTACATCCACGTCATCGAGGG
CGCCACCGGCGGGCCGCGCGACGTCGCGCCGTTCGACTATGCCTCGCTGCGCAAGCGCTTCAGCGGGACCTACATCGCCA
ACAACGGCTACGACCTCGCGCTCGCCACCAAGCAGCTCGACGCCGGCGCCGCCGACCTGATCGCGTTCGGCCGTCCGTTC
ATCGCCAATCCCGATCTGGTCGAGCGGCTCAAGGCCGACGCCCCGCTCAACGAGCTCGACCGCGCCACGCTCTATGGCGG
CGGCGAGAAGGGCTACACGGACTACCCGACGCTTGGGTCCGCGCAAGCTGCGGAGTGA

Upstream 100 bases:

>100_bases
CTTGCGCGGAACCCACCCGCAATTTTGAATGGTTCGGGGCATTGTGCCGTCTGCGGCAATGCACAATTCACCCTCACCTG
TCCCCTCAAGGAAAGCGCAG

Downstream 100 bases:

>100_bases
TCTCGTAGGGTGGGCAAAGCGCCGCCGCCAGGCGGCGTGTGTCCACCATGCATCGGTGCGCGCGGTGGGAATGGTGGGCA
CGGCGCGCGAGGCAGCTCGG

Product: N-ethylmaleimide reductase, FMN-linked

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSTSKLFEPTKLGPITLANRVVMAPLTRNRAVEGLAPGPLTATYYAQRASAGLLISEASQVSQQGQGYQDTPGIYSKAQI
AGWRKVTDAVHAKGGRIFIQLWHVGRISHVSLQPDGGAPVAPSAIAAKTKTFVGGTFADVSTPRALELAEIPGIVEAFKQ
AALNAIEAGFDGIEIHGANGYLLDQFAKDGANKRTDAYGGSIENRARLMIEVAKAVSGAIGADRTGIRLSPVTPANDVSD
SNPQALFDYIVDQLNALKLVYIHVIEGATGGPRDVAPFDYASLRKRFSGTYIANNGYDLALATKQLDAGAADLIAFGRPF
IANPDLVERLKADAPLNELDRATLYGGGEKGYTDYPTLGSAQAAE

Sequences:

>Translated_365_residues
MSTSKLFEPTKLGPITLANRVVMAPLTRNRAVEGLAPGPLTATYYAQRASAGLLISEASQVSQQGQGYQDTPGIYSKAQI
AGWRKVTDAVHAKGGRIFIQLWHVGRISHVSLQPDGGAPVAPSAIAAKTKTFVGGTFADVSTPRALELAEIPGIVEAFKQ
AALNAIEAGFDGIEIHGANGYLLDQFAKDGANKRTDAYGGSIENRARLMIEVAKAVSGAIGADRTGIRLSPVTPANDVSD
SNPQALFDYIVDQLNALKLVYIHVIEGATGGPRDVAPFDYASLRKRFSGTYIANNGYDLALATKQLDAGAADLIAFGRPF
IANPDLVERLKADAPLNELDRATLYGGGEKGYTDYPTLGSAQAAE
>Mature_364_residues
STSKLFEPTKLGPITLANRVVMAPLTRNRAVEGLAPGPLTATYYAQRASAGLLISEASQVSQQGQGYQDTPGIYSKAQIA
GWRKVTDAVHAKGGRIFIQLWHVGRISHVSLQPDGGAPVAPSAIAAKTKTFVGGTFADVSTPRALELAEIPGIVEAFKQA
ALNAIEAGFDGIEIHGANGYLLDQFAKDGANKRTDAYGGSIENRARLMIEVAKAVSGAIGADRTGIRLSPVTPANDVSDS
NPQALFDYIVDQLNALKLVYIHVIEGATGGPRDVAPFDYASLRKRFSGTYIANNGYDLALATKQLDAGAADLIAFGRPFI
ANPDLVERLKADAPLNELDRATLYGGGEKGYTDYPTLGSAQAAE

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=51.2328767123288, Blast_Score=346, Evalue=1e-96,
Organism=Escherichia coli, GI1789463, Length=352, Percent_Identity=30.9659090909091, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17559802, Length=293, Percent_Identity=29.0102389078498, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17559804, Length=271, Percent_Identity=30.9963099630996, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17565138, Length=290, Percent_Identity=26.8965517241379, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=276, Percent_Identity=27.536231884058, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=193, Percent_Identity=32.1243523316062, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=265, Percent_Identity=30.188679245283, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17566914, Length=79, Percent_Identity=45.5696202531646, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=387, Percent_Identity=36.6925064599483, Blast_Score=213, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6321973, Length=391, Percent_Identity=36.5728900255754, Blast_Score=205, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38516; Mature: 38385

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSKLFEPTKLGPITLANRVVMAPLTRNRAVEGLAPGPLTATYYAQRASAGLLISEASQ
CCCCCCCCCCCCCCEECCCCEEEEECHHCCCCCCCCCCCCCHHHHHHHCCCCEEEHHHHH
VSQQGQGYQDTPGIYSKAQIAGWRKVTDAVHAKGGRIFIQLWHVGRISHVSLQPDGGAPV
HHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCC
APSAIAAKTKTFVGGTFADVSTPRALELAEIPGIVEAFKQAALNAIEAGFDGIEIHGANG
CCHHHHHHHHHHCCCEECCCCCCCCEEHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCC
YLLDQFAKDGANKRTDAYGGSIENRARLMIEVAKAVSGAIGADRTGIRLSPVTPANDVSD
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCC
SNPQALFDYIVDQLNALKLVYIHVIEGATGGPRDVAPFDYASLRKRFSGTYIANNGYDLA
CCHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEE
LATKQLDAGAADLIAFGRPFIANPDLVERLKADAPLNELDRATLYGGGEKGYTDYPTLGS
EEECHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHCCCEEECCCCCCCCCCCCCCC
AQAAE
CCCCC
>Mature Secondary Structure 
STSKLFEPTKLGPITLANRVVMAPLTRNRAVEGLAPGPLTATYYAQRASAGLLISEASQ
CCCCCCCCCCCCCEECCCCEEEEECHHCCCCCCCCCCCCCHHHHHHHCCCCEEEHHHHH
VSQQGQGYQDTPGIYSKAQIAGWRKVTDAVHAKGGRIFIQLWHVGRISHVSLQPDGGAPV
HHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCC
APSAIAAKTKTFVGGTFADVSTPRALELAEIPGIVEAFKQAALNAIEAGFDGIEIHGANG
CCHHHHHHHHHHCCCEECCCCCCCCEEHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCC
YLLDQFAKDGANKRTDAYGGSIENRARLMIEVAKAVSGAIGADRTGIRLSPVTPANDVSD
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCC
SNPQALFDYIVDQLNALKLVYIHVIEGATGGPRDVAPFDYASLRKRFSGTYIANNGYDLA
CCHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEE
LATKQLDAGAADLIAFGRPFIANPDLVERLKADAPLNELDRATLYGGGEKGYTDYPTLGS
EEECHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHCCCEEECCCCCCCCCCCCCCC
AQAAE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]