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The map label for this gene is oprF [H]

Identifier: 146338950

GI number: 146338950

Start: 2017333

End: 2017929

Strand: Reverse

Name: oprF [H]

Synonym: BRADO1893

Alternate gene names: 146338950

Gene position: 2017929-2017333 (Counterclockwise)

Preceding gene: 146338955

Following gene: 146338947

Centisome position: 27.06

GC content: 65.66

Gene sequence:

>597_bases
ATGATGAGCGCAGGCGCGGCTTTCGCCGGCGAGGCCGTGACTGCGGACCAGATCCTGAACGCGCTGAAGCCCAAGTCGGT
CACGCGCGGCATCACCGTCGGACCGCAGACGGCCCAGCCGGTCGATGCCGCGGCGCAGGCCAAGCAGGCCGCGCTGCTCG
ACAGCGTCCGGAACCGCAAGACGCGCTCGCTGTCGCTCGGCGAGCGCGAGCAAATCGCGGAACTGGCCGCAAGCAAGCCG
AAGATCGATCTCGAGATCCAGTTCGACTACAACTCCGCCGAGATCAGCAAGGGCTCGATGCAGGCTGTTCAGGAGCTTGG
CAAGGCGCTGTCGGATCCGAACCTGAAGGGCTCGACCTTCGTCGTCGCCGGCCACACCGACGGTATCGGCAGCGACGGCT
TCAACCAGGATCTTTCCGAGCGGCGCGCCGACACCATCAAGCGCTATCTGGTCGAGAAGTACGGCCTCGTCGGCCAGGAC
CTCGTCGCCGTCGGCTACGGCAAGACCAAGCTGAAGGACGCCGCCAATCCGGCCGATCCGCTTAACCGCCGCGTCCAGGT
CGTCAACATGGATACCAAGACCGCGGCTTCGAAGTAG

Upstream 100 bases:

>100_bases
AGCATCCAATGGCCATGAGCCATGACGACCGAGGACCACGACGATGACCCCATATTTTGAACGTTTTGCAGTCCGGGCGG
CGGCGTTTTCCGCCGTGCTG

Downstream 100 bases:

>100_bases
GCGTCGGTCATGATGCGCCGCCACGGCAGCGCATCATGATCCGACTGTTTTCGTGCGCATGATGCGTCCGGAAAACCGGC
GTCTCCTTTTCCGGAGCGTG

Product: putative outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MMSAGAAFAGEAVTADQILNALKPKSVTRGITVGPQTAQPVDAAAQAKQAALLDSVRNRKTRSLSLGEREQIAELAASKP
KIDLEIQFDYNSAEISKGSMQAVQELGKALSDPNLKGSTFVVAGHTDGIGSDGFNQDLSERRADTIKRYLVEKYGLVGQD
LVAVGYGKTKLKDAANPADPLNRRVQVVNMDTKTAASK

Sequences:

>Translated_198_residues
MMSAGAAFAGEAVTADQILNALKPKSVTRGITVGPQTAQPVDAAAQAKQAALLDSVRNRKTRSLSLGEREQIAELAASKP
KIDLEIQFDYNSAEISKGSMQAVQELGKALSDPNLKGSTFVVAGHTDGIGSDGFNQDLSERRADTIKRYLVEKYGLVGQD
LVAVGYGKTKLKDAANPADPLNRRVQVVNMDTKTAASK
>Mature_198_residues
MMSAGAAFAGEAVTADQILNALKPKSVTRGITVGPQTAQPVDAAAQAKQAALLDSVRNRKTRSLSLGEREQIAELAASKP
KIDLEIQFDYNSAEISKGSMQAVQELGKALSDPNLKGSTFVVAGHTDGIGSDGFNQDLSERRADTIKRYLVEKYGLVGQD
LVAVGYGKTKLKDAANPADPLNRRVQVVNMDTKTAASK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Mi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 20988; Mature: 20988

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSAGAAFAGEAVTADQILNALKPKSVTRGITVGPQTAQPVDAAAQAKQAALLDSVRNRK
CCCCCCCCCCCCCCHHHHHHHHCCCHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCH
TRSLSLGEREQIAELAASKPKIDLEIQFDYNSAEISKGSMQAVQELGKALSDPNLKGSTF
HHCCCCCCHHHHHHHHHCCCCEEEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCCCEE
VVAGHTDGIGSDGFNQDLSERRADTIKRYLVEKYGLVGQDLVAVGYGKTKLKDAANPADP
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCHHHHCCCCCCCC
LNRRVQVVNMDTKTAASK
CCCEEEEEECCHHHCCCC
>Mature Secondary Structure
MMSAGAAFAGEAVTADQILNALKPKSVTRGITVGPQTAQPVDAAAQAKQAALLDSVRNRK
CCCCCCCCCCCCCCHHHHHHHHCCCHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCH
TRSLSLGEREQIAELAASKPKIDLEIQFDYNSAEISKGSMQAVQELGKALSDPNLKGSTF
HHCCCCCCHHHHHHHHHCCCCEEEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCCCEE
VVAGHTDGIGSDGFNQDLSERRADTIKRYLVEKYGLVGQDLVAVGYGKTKLKDAANPADP
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCHHHHCCCCCCCC
LNRRVQVVNMDTKTAASK
CCCEEEEEECCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]