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The map label for this gene is ybiS [H]

Identifier: 146338532

GI number: 146338532

Start: 1556548

End: 1557081

Strand: Reverse

Name: ybiS [H]

Synonym: BRADO1451

Alternate gene names: 146338532

Gene position: 1557081-1556548 (Counterclockwise)

Preceding gene: 146338535

Following gene: 146338531

Centisome position: 20.88

GC content: 67.6

Gene sequence:

>534_bases
TTGGCGAGTGCCGCGACGGCACACGCCCAGTTCTTCAATAATGGAGCGGACCCGTTCAGCGCGGTGACGAACGGCTATCA
GAGCGGCGTCAGCCCGATTCCGCGCCAGACCGTGATGTTCGACAGCAACTACGCGCCCGGCACGATCGTCGTGAACACGT
CGGAGCGCCGGCTCTATCTGGTGCTCCAGGGCGGCCAGGCGCTGCGCTACGGCATCGGCGTCGGCCGTGACGGGTTCCGC
TGGAGCGGCGTGCACCGCATCACCGCCAAGAAGGAATGGCCGGCCTGGACCCCGCCGGCGCAGATGCTGCGTCGACGGCC
CGACCTGCCGCGCCACATGGCCGGCGGCATCGAAAACCCGCTCGGCGCGCGCGCGATGTATCTCGGCTCGACGCTGTACC
GCATCCACGGCTCCAACGAGCCGGAGACGATCGGCCAGGCCGTGTCGTCGGGCTGCTTCCGCATGACCAATGACGATGTC
GCCGATCTCTACGACCGCGTCTCGGTCGGCACCACGGTGATCGTCAAGAACTGA

Upstream 100 bases:

>100_bases
GCGAAATGAGGCGGACTATGGCCTCTCTGCGACCGATGATGGATGCCAGGGGATGGAAATGAAGCGGTATTTTGCTTGGG
GAATTGCTGCAATGGCGACG

Downstream 100 bases:

>100_bases
GGCCTGACAGATCTGGATGATGTCGGAAGGTAAGAGCGTCCGTCCGGGCCGGAAGCGTCCCCGCTTCCGGCTTTCGTCAA
GGACCGCGGCGGTCACCTTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MASAATAHAQFFNNGADPFSAVTNGYQSGVSPIPRQTVMFDSNYAPGTIVVNTSERRLYLVLQGGQALRYGIGVGRDGFR
WSGVHRITAKKEWPAWTPPAQMLRRRPDLPRHMAGGIENPLGARAMYLGSTLYRIHGSNEPETIGQAVSSGCFRMTNDDV
ADLYDRVSVGTTVIVKN

Sequences:

>Translated_177_residues
MASAATAHAQFFNNGADPFSAVTNGYQSGVSPIPRQTVMFDSNYAPGTIVVNTSERRLYLVLQGGQALRYGIGVGRDGFR
WSGVHRITAKKEWPAWTPPAQMLRRRPDLPRHMAGGIENPLGARAMYLGSTLYRIHGSNEPETIGQAVSSGCFRMTNDDV
ADLYDRVSVGTTVIVKN
>Mature_176_residues
ASAATAHAQFFNNGADPFSAVTNGYQSGVSPIPRQTVMFDSNYAPGTIVVNTSERRLYLVLQGGQALRYGIGVGRDGFRW
SGVHRITAKKEWPAWTPPAQMLRRRPDLPRHMAGGIENPLGARAMYLGSTLYRIHGSNEPETIGQAVSSGCFRMTNDDVA
DLYDRVSVGTTVIVKN

Specific function: Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family [H]

Homologues:

Organism=Escherichia coli, GI1787040, Length=150, Percent_Identity=38, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI1788299, Length=172, Percent_Identity=36.6279069767442, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI1787356, Length=176, Percent_Identity=34.6590909090909, Blast_Score=83, Evalue=8e-18,
Organism=Escherichia coli, GI1787968, Length=154, Percent_Identity=38.3116883116883, Blast_Score=80, Evalue=8e-17,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005490 [H]

Pfam domain/function: PF03734 YkuD [H]

EC number: NA

Molecular weight: Translated: 19302; Mature: 19171

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAATAHAQFFNNGADPFSAVTNGYQSGVSPIPRQTVMFDSNYAPGTIVVNTSERRLYL
CCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEECCCCEEEE
VLQGGQALRYGIGVGRDGFRWSGVHRITAKKEWPAWTPPAQMLRRRPDLPRHMAGGIENP
EEECCCEEEECCCCCCCCCEECCEEEEEECCCCCCCCCHHHHHHCCCCCCHHHHCCCCCC
LGARAMYLGSTLYRIHGSNEPETIGQAVSSGCFRMTNDDVADLYDRVSVGTTVIVKN
CCCHHHHHCCEEEEEECCCCHHHHHHHHHCCCEECCCCCHHHHHHHHCCCEEEEEEC
>Mature Secondary Structure 
ASAATAHAQFFNNGADPFSAVTNGYQSGVSPIPRQTVMFDSNYAPGTIVVNTSERRLYL
CCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEECCCCEEEE
VLQGGQALRYGIGVGRDGFRWSGVHRITAKKEWPAWTPPAQMLRRRPDLPRHMAGGIENP
EEECCCEEEECCCCCCCCCEECCEEEEEECCCCCCCCCHHHHHHCCCCCCHHHHCCCCCC
LGARAMYLGSTLYRIHGSNEPETIGQAVSSGCFRMTNDDVADLYDRVSVGTTVIVKN
CCCHHHHHCCEEEEEECCCCHHHHHHHHHCCCEECCCCCHHHHHHHHCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]