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The map label for this gene is acyI [H]

Identifier: 146338482

GI number: 146338482

Start: 1495603

End: 1497276

Strand: Reverse

Name: acyI [H]

Synonym: BRADO1399

Alternate gene names: 146338482

Gene position: 1497276-1495603 (Counterclockwise)

Preceding gene: 146338485

Following gene: 146338478

Centisome position: 20.08

GC content: 69.71

Gene sequence:

>1674_bases
ATGGCTTCGTCCAGCCAGCGCATCGCCAATTTCAGCTGCGAGAAGCAGCCGGCCTCCGGCTCACGCGGCATGGTGGTGAC
CAACCATCCGCTCGCATCGAGCGCCGGCGCCGAGATGCTGATGCGCGACGGCAATGCGATCGACGCCGCCGTGGCGGCAC
TGTTCGCTCTCACGGTGGTCGAGCCGATGATGGTCGGCCTTCTCGGCGGCGGTCTGTCGCATATCCGCCTGGCCGACGGC
CGCCACATCGTGCTCGACGGCCTGAGCACCGCGCCATTGCGCGCGACACCTCACATGTACGATCTGGTGTCGGACGAGAT
CGCGCTGGCGCGCGAGACACGCGGCCGGCGCAACGTCGTCGGCGCGCTCGCCGTCGCCGTGCCTGGTGCGCTGAAGGGAT
GGTGCGAGGCGCTGGCGCGCTTCGGCCGGCTGCCGCTGGCCGAAGTGATGGCGCCGGCGATCCGGCTCGCCGAGCACGGC
TTCATCGCCAGCCCCTATCTCAACGACTGCGTGCGGCTGGTCGCGAGCGATCTGGCGCGCGACCCGTATCTCGCCGCGTT
GTTCCTGCCTGACGGCGAGCCGGTCAAAGCGGGGGCGCGCGTGATCCAGGCCGACTATGCGGCATCGCTGTGGCTGATCG
CACGAGAGGGGCCGGAGGCGCTGTATGGCGGGCCGCTCGGCGCCGCGCTTGCGGCCTACATGGCGACGAGCGGCGGCCTC
ATCGACGCGGCTGATCTCGCCGCTTACGCGGTGATCGCGCGCGCGCCGATCCGCGGCCTCTATCGCGGCTATGAGATCAT
CGGGCCGCCCCCGCCGTCATCGAGCGGCGTGCACATCGTGCAGATGCTGAACATCCTCGAAGGCTTCGACGTCGCCGCGC
TCGGCTTCGGCTCGGCGGATGGCGCTCATCTGCTCGCCGAAGCCTTGAAGATCGCGTTCGCCGACCGCGCGGTGGCGACC
GCCGATCCCGCATTCGTCGACGTCCCGATCGAACGCCTGACATCGAAGGCCTATGCGGCCGAGCGGCGGGCCGAGATCGA
CATGGCCAAGGCCGGGAGCTTCGGCCCGGGCCTTTCGCCGACTGAATCGGCCAACACCACGCACGTCACGGTCGCCGACG
CCGAGGGCAATGTCGTGGCTTCGACGCAAACCATCAACGGTCTGTTCGGCGCCTGCGTGCAGATCCCCGGCACCGGCATG
ACCGCCAACAACTACATGTACAATTTCGATCCGCATCCCGGCCGCGCGCTGTCGATCGCTCCTGGCAAGCGCGTCTTCAC
CTCGATGGCTCCGATGATGGCGGTACGGGACGGCAAGCTGCGCTACGCGCTCGGCCTGCCCGGCGGCCTGCGCATCTTCC
CCTCCGCCTTCCAGGCGCTGGTCAACCTGATCGACCATGGCATGTCGCTGCAGGAGGCGGTCGAGGCGCCGCGGCTGTGG
ACGCAGGGTGGTGTGCTCGAGCTCGAGCCGGGCTTTCCCGAACATGTCGCGCAAGCGCTCGCCGCGCGCGGCCACGAGAT
CAAGCGCGAGCCGGTCGTGGCCGGCGGCATGAACGCGATCGCCTTCAACGACGACGGCACCCTGACGGGCGCGGCGTGCT
GGCGCGCCGACGGCACGCCGATCGCGATCTCCGGCGGGAGAGCGCGCGCGGGACTGAGGTTCGCGATCGCCTGA

Upstream 100 bases:

>100_bases
GTTCATCGCTGCGGGGCCGCCCTTCCCCTGGCGCGGCGGATGGGTCAGGATTGCAAGCAACAACCTCGCGGCGTCAGCCG
TTCGACAGAGGAGACATGGG

Downstream 100 bases:

>100_bases
CGGGCCGATTTGCATCATCGCACTCCTCATGGTGAGGAGCGCCGCAGGCGCGTCTCGAACCATGAGGCCCGAGCAGTGAG
TGGCCTCGCCCTTCGAGACG

Product: gamma-glutamyltranspeptidase family protein, ACY1 bifunctional acylase

Products: NA

Alternate protein names: Cephalosporin acylase; GL-7ACA acylase; Gamma-glutamyltranspeptidase; GGT [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MASSSQRIANFSCEKQPASGSRGMVVTNHPLASSAGAEMLMRDGNAIDAAVAALFALTVVEPMMVGLLGGGLSHIRLADG
RHIVLDGLSTAPLRATPHMYDLVSDEIALARETRGRRNVVGALAVAVPGALKGWCEALARFGRLPLAEVMAPAIRLAEHG
FIASPYLNDCVRLVASDLARDPYLAALFLPDGEPVKAGARVIQADYAASLWLIAREGPEALYGGPLGAALAAYMATSGGL
IDAADLAAYAVIARAPIRGLYRGYEIIGPPPPSSSGVHIVQMLNILEGFDVAALGFGSADGAHLLAEALKIAFADRAVAT
ADPAFVDVPIERLTSKAYAAERRAEIDMAKAGSFGPGLSPTESANTTHVTVADAEGNVVASTQTINGLFGACVQIPGTGM
TANNYMYNFDPHPGRALSIAPGKRVFTSMAPMMAVRDGKLRYALGLPGGLRIFPSAFQALVNLIDHGMSLQEAVEAPRLW
TQGGVLELEPGFPEHVAQALAARGHEIKREPVVAGGMNAIAFNDDGTLTGAACWRADGTPIAISGGRARAGLRFAIA

Sequences:

>Translated_557_residues
MASSSQRIANFSCEKQPASGSRGMVVTNHPLASSAGAEMLMRDGNAIDAAVAALFALTVVEPMMVGLLGGGLSHIRLADG
RHIVLDGLSTAPLRATPHMYDLVSDEIALARETRGRRNVVGALAVAVPGALKGWCEALARFGRLPLAEVMAPAIRLAEHG
FIASPYLNDCVRLVASDLARDPYLAALFLPDGEPVKAGARVIQADYAASLWLIAREGPEALYGGPLGAALAAYMATSGGL
IDAADLAAYAVIARAPIRGLYRGYEIIGPPPPSSSGVHIVQMLNILEGFDVAALGFGSADGAHLLAEALKIAFADRAVAT
ADPAFVDVPIERLTSKAYAAERRAEIDMAKAGSFGPGLSPTESANTTHVTVADAEGNVVASTQTINGLFGACVQIPGTGM
TANNYMYNFDPHPGRALSIAPGKRVFTSMAPMMAVRDGKLRYALGLPGGLRIFPSAFQALVNLIDHGMSLQEAVEAPRLW
TQGGVLELEPGFPEHVAQALAARGHEIKREPVVAGGMNAIAFNDDGTLTGAACWRADGTPIAISGGRARAGLRFAIA
>Mature_556_residues
ASSSQRIANFSCEKQPASGSRGMVVTNHPLASSAGAEMLMRDGNAIDAAVAALFALTVVEPMMVGLLGGGLSHIRLADGR
HIVLDGLSTAPLRATPHMYDLVSDEIALARETRGRRNVVGALAVAVPGALKGWCEALARFGRLPLAEVMAPAIRLAEHGF
IASPYLNDCVRLVASDLARDPYLAALFLPDGEPVKAGARVIQADYAASLWLIAREGPEALYGGPLGAALAAYMATSGGLI
DAADLAAYAVIARAPIRGLYRGYEIIGPPPPSSSGVHIVQMLNILEGFDVAALGFGSADGAHLLAEALKIAFADRAVATA
DPAFVDVPIERLTSKAYAAERRAEIDMAKAGSFGPGLSPTESANTTHVTVADAEGNVVASTQTINGLFGACVQIPGTGMT
ANNYMYNFDPHPGRALSIAPGKRVFTSMAPMMAVRDGKLRYALGLPGGLRIFPSAFQALVNLIDHGMSLQEAVEAPRLWT
QGGVLELEPGFPEHVAQALAARGHEIKREPVVAGGMNAIAFNDDGTLTGAACWRADGTPIAISGGRARAGLRFAIA

Specific function: Besides the cephalosporin acylase I activity which converts GL-7ACA into 7-ACA; this enzyme displays some gamma glutamyltranspeptidase activity [H]

COG id: COG0405

COG function: function code E; Gamma-glutamyltransferase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI73915092, Length=522, Percent_Identity=29.5019157088123, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI73915090, Length=522, Percent_Identity=29.5019157088123, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI73915096, Length=522, Percent_Identity=29.5019157088123, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI73915094, Length=522, Percent_Identity=29.5019157088123, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI113429324, Length=496, Percent_Identity=28.2258064516129, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI153266885, Length=537, Percent_Identity=27.9329608938547, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI153266901, Length=538, Percent_Identity=27.8810408921933, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI113429326, Length=501, Percent_Identity=27.9441117764471, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI109148539, Length=523, Percent_Identity=30.2103250478011, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI153266907, Length=536, Percent_Identity=26.865671641791, Blast_Score=146, Evalue=6e-35,
Organism=Homo sapiens, GI30581157, Length=191, Percent_Identity=31.413612565445, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI30581155, Length=191, Percent_Identity=31.413612565445, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI1789856, Length=538, Percent_Identity=39.0334572490706, Blast_Score=356, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI17506189, Length=526, Percent_Identity=29.6577946768061, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI212646519, Length=525, Percent_Identity=26.6666666666667, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI72000294, Length=525, Percent_Identity=26.6666666666667, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI193208245, Length=494, Percent_Identity=26.5182186234818, Blast_Score=145, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI193208247, Length=494, Percent_Identity=26.5182186234818, Blast_Score=145, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17542894, Length=511, Percent_Identity=27.7886497064579, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17564448, Length=427, Percent_Identity=24.824355971897, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323330, Length=510, Percent_Identity=23.7254901960784, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI161077880, Length=529, Percent_Identity=29.8676748582231, Blast_Score=192, Evalue=5e-49,
Organism=Drosophila melanogaster, GI161077878, Length=529, Percent_Identity=29.8676748582231, Blast_Score=192, Evalue=6e-49,
Organism=Drosophila melanogaster, GI24642670, Length=529, Percent_Identity=29.8676748582231, Blast_Score=192, Evalue=7e-49,
Organism=Drosophila melanogaster, GI24642666, Length=529, Percent_Identity=29.8676748582231, Blast_Score=192, Evalue=7e-49,
Organism=Drosophila melanogaster, GI45551474, Length=529, Percent_Identity=29.8676748582231, Blast_Score=192, Evalue=7e-49,
Organism=Drosophila melanogaster, GI161077496, Length=511, Percent_Identity=28.5714285714286, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI18859893, Length=524, Percent_Identity=28.4351145038168, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI18859741, Length=517, Percent_Identity=25.9187620889749, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000101 [H]

Pfam domain/function: PF01019 G_glu_transpept [H]

EC number: =2.3.2.2 [H]

Molecular weight: Translated: 57912; Mature: 57781

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00462 G_GLU_TRANSPEPTIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSSQRIANFSCEKQPASGSRGMVVTNHPLASSAGAEMLMRDGNAIDAAVAALFALTVV
CCCCCCCHHCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
EPMMVGLLGGGLSHIRLADGRHIVLDGLSTAPLRATPHMYDLVSDEIALARETRGRRNVV
HHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHH
GALAVAVPGALKGWCEALARFGRLPLAEVMAPAIRLAEHGFIASPYLNDCVRLVASDLAR
HHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
DPYLAALFLPDGEPVKAGARVIQADYAASLWLIAREGPEALYGGPLGAALAAYMATSGGL
CCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEEEECCCCHHCCCCHHHHHHHHHHCCCCC
IDAADLAAYAVIARAPIRGLYRGYEIIGPPPPSSSGVHIVQMLNILEGFDVAALGFGSAD
EEHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCC
GAHLLAEALKIAFADRAVATADPAFVDVPIERLTSKAYAAERRAEIDMAKAGSFGPGLSP
HHHHHHHHHHHHHHCCHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCC
TESANTTHVTVADAEGNVVASTQTINGLFGACVQIPGTGMTANNYMYNFDPHPGRALSIA
CCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHEECCCCCCCCCCEEEECCCCCCCEEEEC
PGKRVFTSMAPMMAVRDGKLRYALGLPGGLRIFPSAFQALVNLIDHGMSLQEAVEAPRLW
CCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCEE
TQGGVLELEPGFPEHVAQALAARGHEIKREPVVAGGMNAIAFNDDGTLTGAACWRADGTP
CCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEECCCEEEEECCCCCEEEEEEECCCCCE
IAISGGRARAGLRFAIA
EEEECCCCCCCEEEEEC
>Mature Secondary Structure 
ASSSQRIANFSCEKQPASGSRGMVVTNHPLASSAGAEMLMRDGNAIDAAVAALFALTVV
CCCCCCHHCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHH
EPMMVGLLGGGLSHIRLADGRHIVLDGLSTAPLRATPHMYDLVSDEIALARETRGRRNVV
HHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHH
GALAVAVPGALKGWCEALARFGRLPLAEVMAPAIRLAEHGFIASPYLNDCVRLVASDLAR
HHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
DPYLAALFLPDGEPVKAGARVIQADYAASLWLIAREGPEALYGGPLGAALAAYMATSGGL
CCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEEEECCCCHHCCCCHHHHHHHHHHCCCCC
IDAADLAAYAVIARAPIRGLYRGYEIIGPPPPSSSGVHIVQMLNILEGFDVAALGFGSAD
EEHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCC
GAHLLAEALKIAFADRAVATADPAFVDVPIERLTSKAYAAERRAEIDMAKAGSFGPGLSP
HHHHHHHHHHHHHHCCHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCC
TESANTTHVTVADAEGNVVASTQTINGLFGACVQIPGTGMTANNYMYNFDPHPGRALSIA
CCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHEECCCCCCCCCCEEEECCCCCCCEEEEC
PGKRVFTSMAPMMAVRDGKLRYALGLPGGLRIFPSAFQALVNLIDHGMSLQEAVEAPRLW
CCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCEE
TQGGVLELEPGFPEHVAQALAARGHEIKREPVVAGGMNAIAFNDDGTLTGAACWRADGTP
CCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEECCCEEEEECCCCCEEEEEEECCCCCE
IAISGGRARAGLRFAIA
EEEECCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3680178 [H]