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The map label for this gene is afr [H]

Identifier: 146338467

GI number: 146338467

Start: 1475480

End: 1476517

Strand: Reverse

Name: afr [H]

Synonym: BRADO1384

Alternate gene names: 146338467

Gene position: 1476517-1475480 (Counterclockwise)

Preceding gene: 146338468

Following gene: 146338461

Centisome position: 19.8

GC content: 68.3

Gene sequence:

>1038_bases
TTGATCAGTCTCCCTGTCCGTTTGGCCGTCGCCGGAGCGGGTCTCATCGGCCGGCGTCACATCGAGCAGATCGACGCCTG
TCCGGAAGCGGTGCTGACGGCGATCATCGATCCGGCGCCCGCCGCCAACGAGCTTGCGCAAGCGCGCGGCGTCCGCTGTT
ATTCGTCGCTTGACGCGCTTCCGGCCGAGCACCGGCCCGACGGCGTGGTCATCGCCACGCCGAACCAGCTGCACGTCGCC
AACGGCATGGCCTGCCTGGCCGCGGGACTGCCGGCGCTGATCGAGAAGCCCCTGGCCGATGACGTCGCAGCGGCGCAGAC
ACTGGTCGAGGCCTTCGAACGCGCCGGCGTGCCGCTCCTGACCGGTCATCACCGCCGCCACAATCCGATGATCCAGCGCG
CCAAGGCCGAGATCGACACCGGCCGTCTCGGCCGGATCGTCGCCATCCATGGCGTATTCTGGCTGATCAAGCCGCACGAT
TATTTCGCGCCGGCCTGGCGCCGCGAGGCCGGCGCCGGCCCGGTGCTGATCAATCTCAGCCACGACATCGATTTGCTACG
CTATCTCTGCGGCGACATCGTCGCAGTGCAGGCGGCACAGTCCAACCAGGTCCGCGGCCATGCCGTCGAGGATACGGCGG
CGATGATCCTGCACTTCGAGTCCGGCGCGCTCGGCACGTTCAGCGTGTCCGATACGGTGCAGGCGCCCTGGAGCTACGAG
TTCACCAGTGGCGAGAACCCGGCCTACAGCCGGACGCAGGAAAGCTGCTACCAGATCGGCGGCACGAAGGGGTCGCTCGC
GATCCCGCAGCTCGACCTGTGGCATCATCCGGATGCGGCGAGCTGGTGGGCACCGATCGCGCGCGAGCGGCTCAGCTATG
ACATGCAGGAGCCGCTCGCCCGGCAGATTGGGAATTTCTGCGCGGTCATCCGCGGCACGGCCGCTCCGGTGGTCAGCGGT
CGCGAGGGCCTGAACACGCTGCGCGTGATCGCCGCGATCAAGCGAGCCGCGGCGTCGGGAGCTCGTGAGACCGTCTGA

Upstream 100 bases:

>100_bases
CCAAGCGCGCCGACGGTGTCATGGCGGGCTTCGGCACCCAGGGCTATCAGCTCGCGCTCCGGCGGGTCGCCAGGCTGCTC
GACGAGGGCAAGGCCGGCCA

Downstream 100 bases:

>100_bases
GAGTTGCAGCCGCCCGGCGCTATGTTGGGCGTGCACTGAGTAGGCTTGCACTGAGTGGACTTGCACTCACTGGACTTGCG
CTCAATTGACCGGCGCGAAA

Product: NAD(P)-dependent oxidoreductase

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MISLPVRLAVAGAGLIGRRHIEQIDACPEAVLTAIIDPAPAANELAQARGVRCYSSLDALPAEHRPDGVVIATPNQLHVA
NGMACLAAGLPALIEKPLADDVAAAQTLVEAFERAGVPLLTGHHRRHNPMIQRAKAEIDTGRLGRIVAIHGVFWLIKPHD
YFAPAWRREAGAGPVLINLSHDIDLLRYLCGDIVAVQAAQSNQVRGHAVEDTAAMILHFESGALGTFSVSDTVQAPWSYE
FTSGENPAYSRTQESCYQIGGTKGSLAIPQLDLWHHPDAASWWAPIARERLSYDMQEPLARQIGNFCAVIRGTAAPVVSG
REGLNTLRVIAAIKRAAASGARETV

Sequences:

>Translated_345_residues
MISLPVRLAVAGAGLIGRRHIEQIDACPEAVLTAIIDPAPAANELAQARGVRCYSSLDALPAEHRPDGVVIATPNQLHVA
NGMACLAAGLPALIEKPLADDVAAAQTLVEAFERAGVPLLTGHHRRHNPMIQRAKAEIDTGRLGRIVAIHGVFWLIKPHD
YFAPAWRREAGAGPVLINLSHDIDLLRYLCGDIVAVQAAQSNQVRGHAVEDTAAMILHFESGALGTFSVSDTVQAPWSYE
FTSGENPAYSRTQESCYQIGGTKGSLAIPQLDLWHHPDAASWWAPIARERLSYDMQEPLARQIGNFCAVIRGTAAPVVSG
REGLNTLRVIAAIKRAAASGARETV
>Mature_345_residues
MISLPVRLAVAGAGLIGRRHIEQIDACPEAVLTAIIDPAPAANELAQARGVRCYSSLDALPAEHRPDGVVIATPNQLHVA
NGMACLAAGLPALIEKPLADDVAAAQTLVEAFERAGVPLLTGHHRRHNPMIQRAKAEIDTGRLGRIVAIHGVFWLIKPHD
YFAPAWRREAGAGPVLINLSHDIDLLRYLCGDIVAVQAAQSNQVRGHAVEDTAAMILHFESGALGTFSVSDTVQAPWSYE
FTSGENPAYSRTQESCYQIGGTKGSLAIPQLDLWHHPDAASWWAPIARERLSYDMQEPLARQIGNFCAVIRGTAAPVVSG
REGLNTLRVIAAIKRAAASGARETV

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol [H]

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI87082405, Length=274, Percent_Identity=27.007299270073, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 36957; Mature: 36957

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLPVRLAVAGAGLIGRRHIEQIDACPEAVLTAIIDPAPAANELAQARGVRCYSSLDAL
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHCC
PAEHRPDGVVIATPNQLHVANGMACLAAGLPALIEKPLADDVAAAQTLVEAFERAGVPLL
CCCCCCCEEEEECCCEEEHHCCHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEE
TGHHRRHNPMIQRAKAEIDTGRLGRIVAIHGVFWLIKPHDYFAPAWRREAGAGPVLINLS
ECCCCCCCHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCCCCCHHHHCCCCCCEEEEEC
HDIDLLRYLCGDIVAVQAAQSNQVRGHAVEDTAAMILHFESGALGTFSVSDTVQAPWSYE
CCHHHHHHHHHHHHEEECCCCCCCCCCEECCCEEEEEEECCCCCEEEECCCCCCCCCCEE
FTSGENPAYSRTQESCYQIGGTKGSLAIPQLDLWHHPDAASWWAPIARERLSYDMQEPLA
ECCCCCCCCHHHHHHHHHHCCCCCCEECCCEECCCCCCCHHHHHHHHHHHHCCCHHHHHH
RQIGNFCAVIRGTAAPVVSGREGLNTLRVIAAIKRAAASGARETV
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MISLPVRLAVAGAGLIGRRHIEQIDACPEAVLTAIIDPAPAANELAQARGVRCYSSLDAL
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHCC
PAEHRPDGVVIATPNQLHVANGMACLAAGLPALIEKPLADDVAAAQTLVEAFERAGVPLL
CCCCCCCEEEEECCCEEEHHCCHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEE
TGHHRRHNPMIQRAKAEIDTGRLGRIVAIHGVFWLIKPHDYFAPAWRREAGAGPVLINLS
ECCCCCCCHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCCCCCHHHHCCCCCCEEEEEC
HDIDLLRYLCGDIVAVQAAQSNQVRGHAVEDTAAMILHFESGALGTFSVSDTVQAPWSYE
CCHHHHHHHHHHHHEEECCCCCCCCCCEECCCEEEEEEECCCCCEEEECCCCCCCCCCEE
FTSGENPAYSRTQESCYQIGGTKGSLAIPQLDLWHHPDAASWWAPIARERLSYDMQEPLA
ECCCCCCCCHHHHHHHHHHCCCCCCEECCCEECCCCCCCHHHHHHHHHHHHCCCHHHHHH
RQIGNFCAVIRGTAAPVVSGREGLNTLRVIAAIKRAAASGARETV
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]