BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is ephD [H]

Identifier: 146338450

GI number: 146338450

Start: 1452144

End: 1452983

Strand: Reverse

Name: ephD [H]

Synonym: BRADO1366

Alternate gene names: 146338450

Gene position: 1452983-1452144 (Counterclockwise)

Preceding gene: 146338452

Following gene: 146338448

Centisome position: 19.49

GC content: 68.45

Gene sequence:

>840_bases
ATGACGGCAATCGACAAGGCGGCCGCGGCGATCACCGGCGCGGCCAGCGGCATCGGCCGCGCACTGGCGCTGGAAATGGC
CGCGCGCGGTTGTGATCTCGCACTCGCCGACCGCGACGAAGCCGGCCTTGCGAGCGTCGCGGCGGAGCTTGCCGCGTCCG
GCCGCAAGGTCACGATGCACCGGCTGGACGTCAGCGATGCGTCGGCGATCGCGCAATTCGCCGCGGACGCCACGGCTGCG
CATCCCGCTCTCAACATCGTCGTCAACAACGCCGGCGTGGCGCTGTTCGGCGCCTTCCACGAGATCGAGCAGGCCGAGAT
CGAGTGGCTGTTCAATATCAATTTCTGGGGCGTCGTGCACGGCACCCGCGCGTTCCTGCCGCATCTGTCGCGCCAGCCGG
AAGCGCATATCGTCAACCTGTCCTCGATCTTCGGCATCATCGCGCCGCCCGGCCAGTCCGCCTATGCGGCAGCGAAGTTC
GCAGTGCGCGGGTTTTCCGAGAGCCTGCGTCATGAGCTCGCGACGGCGAACAGCCCGGTGAAGCTGTCGGTGGTGCATCC
CGGCGGCGTCGCGACCGCGATCGCGCGCAACGCCCGTGCCGGCACAGGCATGACGGACAATGCGCGCCGCGTGCAGGCAA
TCGAGCGCTTCGAGCGGCTCGCCAAGACCAGCCCTCGCGACGCGGCGCTGCGCATCATCCAGGGCATCGAGCGCAACGAG
CCGCGCATCCTGATCGGCGGCGATGCGCGCTTCATGGACCTGTTGCAGCGGTTCATGCCCGGCACCTATTGGAAGGTGAT
GGCGAAGCGGCTGGAGAAGGCGGCCGCGAAGGAAGGGTGA

Upstream 100 bases:

>100_bases
GCAAAGCCGGCAGATGGTTCGGTTCCAATGCGAAAAAGTGACGCAAGGTGATCGCGGCATTTGGAACGCGGGCCGCGAAG
CGAAGGGGCAGGATGCGGCA

Downstream 100 bases:

>100_bases
AACTCAGGATAAGCGAAACTTGTCTCGCGGCGCGTGATGCGTCCGAGTTGTGTACCTTCGCCGCCCTCGTCGAGATGGAG
GGCGCAGGGAGAGCCAGGCC

Product: putative short-chain dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTAIDKAAAAITGAASGIGRALALEMAARGCDLALADRDEAGLASVAAELAASGRKVTMHRLDVSDASAIAQFAADATAA
HPALNIVVNNAGVALFGAFHEIEQAEIEWLFNINFWGVVHGTRAFLPHLSRQPEAHIVNLSSIFGIIAPPGQSAYAAAKF
AVRGFSESLRHELATANSPVKLSVVHPGGVATAIARNARAGTGMTDNARRVQAIERFERLAKTSPRDAALRIIQGIERNE
PRILIGGDARFMDLLQRFMPGTYWKVMAKRLEKAAAKEG

Sequences:

>Translated_279_residues
MTAIDKAAAAITGAASGIGRALALEMAARGCDLALADRDEAGLASVAAELAASGRKVTMHRLDVSDASAIAQFAADATAA
HPALNIVVNNAGVALFGAFHEIEQAEIEWLFNINFWGVVHGTRAFLPHLSRQPEAHIVNLSSIFGIIAPPGQSAYAAAKF
AVRGFSESLRHELATANSPVKLSVVHPGGVATAIARNARAGTGMTDNARRVQAIERFERLAKTSPRDAALRIIQGIERNE
PRILIGGDARFMDLLQRFMPGTYWKVMAKRLEKAAAKEG
>Mature_278_residues
TAIDKAAAAITGAASGIGRALALEMAARGCDLALADRDEAGLASVAAELAASGRKVTMHRLDVSDASAIAQFAADATAAH
PALNIVVNNAGVALFGAFHEIEQAEIEWLFNINFWGVVHGTRAFLPHLSRQPEAHIVNLSSIFGIIAPPGQSAYAAAKFA
VRGFSESLRHELATANSPVKLSVVHPGGVATAIARNARAGTGMTDNARRVQAIERFERLAKTSPRDAALRIIQGIERNEP
RILIGGDARFMDLLQRFMPGTYWKVMAKRLEKAAAKEG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI142976729, Length=262, Percent_Identity=29.7709923664122, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI20149619, Length=274, Percent_Identity=28.8321167883212, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI7657478, Length=188, Percent_Identity=34.0425531914894, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI4503817, Length=239, Percent_Identity=29.7071129707113, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI7706318, Length=195, Percent_Identity=31.2820512820513, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI210032110, Length=252, Percent_Identity=28.968253968254, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI40807363, Length=254, Percent_Identity=27.1653543307087, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI44680136, Length=198, Percent_Identity=28.7878787878788, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI44680133, Length=198, Percent_Identity=28.7878787878788, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI17738292, Length=198, Percent_Identity=28.7878787878788, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI150247226, Length=190, Percent_Identity=31.0526315789474, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310128830, Length=196, Percent_Identity=31.6326530612245, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310115468, Length=196, Percent_Identity=31.6326530612245, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI31542939, Length=196, Percent_Identity=32.1428571428571, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI25282469, Length=282, Percent_Identity=26.5957446808511, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI109715829, Length=193, Percent_Identity=27.4611398963731, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI223718074, Length=267, Percent_Identity=25.8426966292135, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4557649, Length=191, Percent_Identity=30.3664921465969, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI22507405, Length=188, Percent_Identity=31.9148936170213, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI157739938, Length=193, Percent_Identity=28.4974093264249, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI32483357, Length=189, Percent_Identity=27.5132275132275, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI19743808, Length=220, Percent_Identity=31.3636363636364, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI32455239, Length=198, Percent_Identity=29.2929292929293, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI5031765, Length=198, Percent_Identity=29.2929292929293, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI40254992, Length=196, Percent_Identity=28.0612244897959, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI224922801, Length=170, Percent_Identity=31.1764705882353, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI4504505, Length=184, Percent_Identity=30.9782608695652, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI40254986, Length=217, Percent_Identity=25.3456221198157, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI50726952, Length=189, Percent_Identity=31.2169312169312, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI4504503, Length=198, Percent_Identity=28.7878787878788, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI38424085, Length=229, Percent_Identity=30.1310043668122, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI87082100, Length=198, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1786701, Length=215, Percent_Identity=30.2325581395349, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787335, Length=188, Percent_Identity=30.3191489361702, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787820, Length=190, Percent_Identity=30.5263157894737, Blast_Score=77, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17567345, Length=163, Percent_Identity=34.3558282208589, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71997402, Length=262, Percent_Identity=27.4809160305344, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17570069, Length=264, Percent_Identity=28.4090909090909, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193203115, Length=184, Percent_Identity=29.8913043478261, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193203117, Length=184, Percent_Identity=29.8913043478261, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17508895, Length=185, Percent_Identity=29.1891891891892, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17555706, Length=200, Percent_Identity=30, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17568967, Length=182, Percent_Identity=31.8681318681319, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=192, Percent_Identity=32.8125, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17564282, Length=193, Percent_Identity=25.3886010362694, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71994604, Length=193, Percent_Identity=29.0155440414508, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32563809, Length=203, Percent_Identity=28.0788177339901, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17507613, Length=203, Percent_Identity=28.0788177339901, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71994600, Length=193, Percent_Identity=32.1243523316062, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=188, Percent_Identity=29.2553191489362, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=199, Percent_Identity=30.1507537688442, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560150, Length=188, Percent_Identity=30.8510638297872, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17551412, Length=213, Percent_Identity=27.6995305164319, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=193, Percent_Identity=35.7512953367876, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322227, Length=257, Percent_Identity=29.5719844357977, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322067, Length=284, Percent_Identity=26.4084507042254, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21358495, Length=276, Percent_Identity=32.6086956521739, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI21355631, Length=172, Percent_Identity=30.8139534883721, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24581726, Length=269, Percent_Identity=27.5092936802974, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24640492, Length=232, Percent_Identity=28.448275862069, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI23397609, Length=188, Percent_Identity=35.1063829787234, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281364892, Length=225, Percent_Identity=27.1111111111111, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356301, Length=170, Percent_Identity=30, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571526, Length=197, Percent_Identity=29.4416243654822, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24644339, Length=192, Percent_Identity=31.7708333333333, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24649181, Length=257, Percent_Identity=22.568093385214, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640442, Length=199, Percent_Identity=32.1608040201005, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24580925, Length=200, Percent_Identity=31.5, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357041, Length=197, Percent_Identity=27.9187817258883, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI116007236, Length=199, Percent_Identity=29.1457286432161, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24647946, Length=211, Percent_Identity=27.4881516587678, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45553409, Length=202, Percent_Identity=28.2178217821782, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24647948, Length=202, Percent_Identity=28.2178217821782, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI221510726, Length=279, Percent_Identity=29.0322580645161, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24641388, Length=200, Percent_Identity=30.5, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI116007238, Length=188, Percent_Identity=29.2553191489362, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI161076933, Length=217, Percent_Identity=31.7972350230415, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24584748, Length=198, Percent_Identity=32.8282828282828, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571387, Length=194, Percent_Identity=28.3505154639175, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24651139, Length=174, Percent_Identity=32.7586206896552, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24643142, Length=184, Percent_Identity=30.9782608695652, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24584752, Length=194, Percent_Identity=28.8659793814433, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648693, Length=194, Percent_Identity=27.319587628866, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355889, Length=194, Percent_Identity=27.319587628866, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24639444, Length=187, Percent_Identity=30.4812834224599, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116007234, Length=199, Percent_Identity=30.6532663316583, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24641232, Length=200, Percent_Identity=28, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI221331218, Length=239, Percent_Identity=23.4309623430962, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24665243, Length=239, Percent_Identity=23.4309623430962, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 29582; Mature: 29451

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIDKAAAAITGAASGIGRALALEMAARGCDLALADRDEAGLASVAAELAASGRKVTMH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCEEEEE
RLDVSDASAIAQFAADATAAHPALNIVVNNAGVALFGAFHEIEQAEIEWLFNINFWGVVH
EECCCHHHHHHHHHHHHHHCCCEEEEEEECCCEEEHHHHHHHHHCCEEEEEECEEEEEEC
GTRAFLPHLSRQPEAHIVNLSSIFGIIAPPGQSAYAAAKFAVRGFSESLRHELATANSPV
CHHHHHHHHCCCCCCEEEEHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
KLSVVHPGGVATAIARNARAGTGMTDNARRVQAIERFERLAKTSPRDAALRIIQGIERNE
EEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
PRILIGGDARFMDLLQRFMPGTYWKVMAKRLEKAAAKEG
CEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TAIDKAAAAITGAASGIGRALALEMAARGCDLALADRDEAGLASVAAELAASGRKVTMH
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCEEEEE
RLDVSDASAIAQFAADATAAHPALNIVVNNAGVALFGAFHEIEQAEIEWLFNINFWGVVH
EECCCHHHHHHHHHHHHHHCCCEEEEEEECCCEEEHHHHHHHHHCCEEEEEECEEEEEEC
GTRAFLPHLSRQPEAHIVNLSSIFGIIAPPGQSAYAAAKFAVRGFSESLRHELATANSPV
CHHHHHHHHCCCCCCEEEEHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
KLSVVHPGGVATAIARNARAGTGMTDNARRVQAIERFERLAKTSPRDAALRIIQGIERNE
EEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
PRILIGGDARFMDLLQRFMPGTYWKVMAKRLEKAAAKEG
CEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]