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The map label for this gene is uvrD [H]

Identifier: 146337792

GI number: 146337792

Start: 701286

End: 703346

Strand: Reverse

Name: uvrD [H]

Synonym: BRADO0671

Alternate gene names: 146337792

Gene position: 703346-701286 (Counterclockwise)

Preceding gene: 146337804

Following gene: 146337789

Centisome position: 9.43

GC content: 67.39

Gene sequence:

>2061_bases
GTGCAGGCGATTGCGGCGACATATCTCGATGATCTCAATCCGGAGCAGCGCCGCGCCGTCGAGCATGGCGGCACGTCCGC
CGCACCGGGCCCGCCGTTGCTGATCATCGCGGGTGCCGGCTCGGGCAAGACCAACACGCTGGCGCATCGCGTCGCACATC
TCCTCGTCAACGGCGCCGATCCGCGCCGCATCCTGCTGATGACATTCTCGCGCCGCGCGGCGAGCGAAATGAGCCGGCGC
GTCGAGCGCATCGCGCGCAAGGTGCTCGGCGGCCAGGCGGAGGCGATCACCGCGGGGCTCACCTGGGCCGGCACGTTTCA
CGGCCTCGGCGCGCGAATGCTGCGCGAATACGCCGAGCGCATCGGCCTCGATCCCGTGTTCACGATCCATGATCGCGAGG
ATTCCGCCGACCTGATGAACCTGGTGCGGCATGAGCGCGGCCTGTCCAACACGCAGCGCCGCTTCCCGACGAAGGGAACT
TGCCTCGCGATCTACTCGCGCTGCGTCAATGCCGACATGCCGCTGGACGAGGCGCTGGTGCGGCATTTTCCATGGTGCGC
TGAATGGGCCGGTGAGCTGAAGGGCTTGTTCGCGGCCTATGTCGAGGCCAAGCAGGCCAACGCCGTGCTCGACTACGACG
ACCTGCTGCTCTACTGGGCGCAGATGATGGCGGATGAAACCATCGCCCGCGAGATCGGCGAGCGCTTCGATCACGTGCTG
GTCGACGAGTATCAGGACACGAACCGGCTGCAATCGCAGATCCTGCTCGGCCTGAAGCCGGATGGTGCAGGCCTTACGGT
GGTCGGCGACGACGCGCAGTCGATCTACGCATTCCGCGCCGCGACCGTGCGCAACATCCTGGAATTTCCCGACCAGTTCA
GCCCGCGCGCCGACATCGTCACGCTCGACCGCAACTACCGCTCGACGCAGCCGATCCTCTCGGCCGCCAACGCCGTGATC
GGGCTGGCGCGCGAGCGCTTCACCAAGAATTTGTGGACCGACCGCACCGCCTCGCGCAAGCCGCAGCTGGTGACGGTGCG
CGACGAGACCGACCAGGCGCGCTACATCGTCGAGCAGGTGCTGGCCAAGCGCGAGGAAGGCATCGTGCTGAAGCAGCAGG
CGGTGCTGTTCCGGACGTCGTCGCACTCGGGGCCGCTGGAGATCGAGCTGACGCGGCGGAACATTCCCTTCGTGAAGTTC
GGCGGCCTGAAATTTCTCGATGCCGCCCATGTCAAGGACATGCTGGCCTGCCTGCGCTTCGTCGAGAACCCGCGCGACCG
CATCGCCGGCTTCCGCGTGCTGCATCTCATTCCCGGCATCGGTCCGGCCTCGGCGCAGCGGCTGCTCGATGCCATCAGTG
AGAGCGCCGATCCGCTTCGGACGTTGTGCGCACAGCCGACGCCGGCCCGCGCCGGCGAGGATTGGACCGCGTTCGTGCGC
ACGCTGGAGCAGTTGCGGAGCTCGGACTGGCCGGCCGATATCGAGCGAATCAGGCACTGGTATCAGCCGCATCTCGAACG
CGTGCACGAGGATGCCGAGATGCGCAGCGCCGATCTGCTGCAGCTCGAACAGATCGCCAGCGGCTATGCCTCGCGCGAGC
GCTTCCTCACCGAGCTCACGCTCGATCCGCCCGATGCGACCTCCGACCAAGCCGGCGTGCCGCTGCTCGACGAGGACTAC
CTGATCCTCTCCACCATCCATTCCGCCAAGGGCATGGAGTGGAACGCGGTGCACGTCCTCAACGTGGTGGATGGCTGCAT
GCCGTCCGATCTCGGCGCCGGCTCCACCGCCGAGCTGGAGGAGGAGCGCCGGCTGCTCTACGTCGCGATGACCCGCGCCA
AGGACGACCTGCACCTGCTGGTGCCGCAGCGCTTCTTCGTCCATGGCCAGCCGGTGCGTGGCGATCGTCATGTCTACGCC
TCGCGCACACGCTTCATTCCGGAAGCGCTGGTGCCGCTGTTCGAGCGCGTGAGCTGGCCGAAGCTGCAGGCGGGGGTGAC
GGTTGCGAGCGCGGGGCCGCGGATTGACCTGGGTGCGCGCATGCGCGAGCGGTGGCGGTGA

Upstream 100 bases:

>100_bases
AAAAGACCGGTTGCGAATCATGTAAAACGCTGCCGCAAGCGGACCATGTCGTTGCACGCGAGGGGCAAATCACTGATTCT
TGCGCGCTGGGAGACGATTC

Downstream 100 bases:

>100_bases
AGCGACGCAGCTGCTACGTAGCTACGGTGCCAGTGACTGTGCCCTCTCCCCTTGTGGGAGAGGGCTACACAGCTGGTAAT
CACAAACTCACTCGGGTGAG

Product: putative ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MQAIAATYLDDLNPEQRRAVEHGGTSAAPGPPLLIIAGAGSGKTNTLAHRVAHLLVNGADPRRILLMTFSRRAASEMSRR
VERIARKVLGGQAEAITAGLTWAGTFHGLGARMLREYAERIGLDPVFTIHDREDSADLMNLVRHERGLSNTQRRFPTKGT
CLAIYSRCVNADMPLDEALVRHFPWCAEWAGELKGLFAAYVEAKQANAVLDYDDLLLYWAQMMADETIAREIGERFDHVL
VDEYQDTNRLQSQILLGLKPDGAGLTVVGDDAQSIYAFRAATVRNILEFPDQFSPRADIVTLDRNYRSTQPILSAANAVI
GLARERFTKNLWTDRTASRKPQLVTVRDETDQARYIVEQVLAKREEGIVLKQQAVLFRTSSHSGPLEIELTRRNIPFVKF
GGLKFLDAAHVKDMLACLRFVENPRDRIAGFRVLHLIPGIGPASAQRLLDAISESADPLRTLCAQPTPARAGEDWTAFVR
TLEQLRSSDWPADIERIRHWYQPHLERVHEDAEMRSADLLQLEQIASGYASRERFLTELTLDPPDATSDQAGVPLLDEDY
LILSTIHSAKGMEWNAVHVLNVVDGCMPSDLGAGSTAELEEERRLLYVAMTRAKDDLHLLVPQRFFVHGQPVRGDRHVYA
SRTRFIPEALVPLFERVSWPKLQAGVTVASAGPRIDLGARMRERWR

Sequences:

>Translated_686_residues
MQAIAATYLDDLNPEQRRAVEHGGTSAAPGPPLLIIAGAGSGKTNTLAHRVAHLLVNGADPRRILLMTFSRRAASEMSRR
VERIARKVLGGQAEAITAGLTWAGTFHGLGARMLREYAERIGLDPVFTIHDREDSADLMNLVRHERGLSNTQRRFPTKGT
CLAIYSRCVNADMPLDEALVRHFPWCAEWAGELKGLFAAYVEAKQANAVLDYDDLLLYWAQMMADETIAREIGERFDHVL
VDEYQDTNRLQSQILLGLKPDGAGLTVVGDDAQSIYAFRAATVRNILEFPDQFSPRADIVTLDRNYRSTQPILSAANAVI
GLARERFTKNLWTDRTASRKPQLVTVRDETDQARYIVEQVLAKREEGIVLKQQAVLFRTSSHSGPLEIELTRRNIPFVKF
GGLKFLDAAHVKDMLACLRFVENPRDRIAGFRVLHLIPGIGPASAQRLLDAISESADPLRTLCAQPTPARAGEDWTAFVR
TLEQLRSSDWPADIERIRHWYQPHLERVHEDAEMRSADLLQLEQIASGYASRERFLTELTLDPPDATSDQAGVPLLDEDY
LILSTIHSAKGMEWNAVHVLNVVDGCMPSDLGAGSTAELEEERRLLYVAMTRAKDDLHLLVPQRFFVHGQPVRGDRHVYA
SRTRFIPEALVPLFERVSWPKLQAGVTVASAGPRIDLGARMRERWR
>Mature_686_residues
MQAIAATYLDDLNPEQRRAVEHGGTSAAPGPPLLIIAGAGSGKTNTLAHRVAHLLVNGADPRRILLMTFSRRAASEMSRR
VERIARKVLGGQAEAITAGLTWAGTFHGLGARMLREYAERIGLDPVFTIHDREDSADLMNLVRHERGLSNTQRRFPTKGT
CLAIYSRCVNADMPLDEALVRHFPWCAEWAGELKGLFAAYVEAKQANAVLDYDDLLLYWAQMMADETIAREIGERFDHVL
VDEYQDTNRLQSQILLGLKPDGAGLTVVGDDAQSIYAFRAATVRNILEFPDQFSPRADIVTLDRNYRSTQPILSAANAVI
GLARERFTKNLWTDRTASRKPQLVTVRDETDQARYIVEQVLAKREEGIVLKQQAVLFRTSSHSGPLEIELTRRNIPFVKF
GGLKFLDAAHVKDMLACLRFVENPRDRIAGFRVLHLIPGIGPASAQRLLDAISESADPLRTLCAQPTPARAGEDWTAFVR
TLEQLRSSDWPADIERIRHWYQPHLERVHEDAEMRSADLLQLEQIASGYASRERFLTELTLDPPDATSDQAGVPLLDEDY
LILSTIHSAKGMEWNAVHVLNVVDGCMPSDLGAGSTAELEEERRLLYVAMTRAKDDLHLLVPQRFFVHGQPVRGDRHVYA
SRTRFIPEALVPLFERVSWPKLQAGVTVASAGPRIDLGARMRERWR

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=674, Percent_Identity=32.4925816023739, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI48994965, Length=661, Percent_Identity=28.8956127080182, Blast_Score=188, Evalue=1e-48,
Organism=Escherichia coli, GI1787196, Length=382, Percent_Identity=27.7486910994764, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6322369, Length=467, Percent_Identity=27.1948608137045, Blast_Score=126, Evalue=1e-29,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76816; Mature: 76816

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAIAATYLDDLNPEQRRAVEHGGTSAAPGPPLLIIAGAGSGKTNTLAHRVAHLLVNGAD
CCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
PRRILLMTFSRRAASEMSRRVERIARKVLGGQAEAITAGLTWAGTFHGLGARMLREYAER
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEECHHHHHHHHHHHHHHHHH
IGLDPVFTIHDREDSADLMNLVRHERGLSNTQRRFPTKGTCLAIYSRCVNADMPLDEALV
CCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHH
RHFPWCAEWAGELKGLFAAYVEAKQANAVLDYDDLLLYWAQMMADETIAREIGERFDHVL
HHCCCHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDEYQDTNRLQSQILLGLKPDGAGLTVVGDDAQSIYAFRAATVRNILEFPDQFSPRADIV
HHHHHHHHHHHHHHEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
TLDRNYRSTQPILSAANAVIGLARERFTKNLWTDRTASRKPQLVTVRDETDQARYIVEQV
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHH
LAKREEGIVLKQQAVLFRTSSHSGPLEIELTRRNIPFVKFGGLKFLDAAHVKDMLACLRF
HHHHHCCEEEEEHEEEEEECCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHH
VENPRDRIAGFRVLHLIPGIGPASAQRLLDAISESADPLRTLCAQPTPARAGEDWTAFVR
HCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHH
TLEQLRSSDWPADIERIRHWYQPHLERVHEDAEMRSADLLQLEQIASGYASRERFLTELT
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LDPPDATSDQAGVPLLDEDYLILSTIHSAKGMEWNAVHVLNVVDGCMPSDLGAGSTAELE
CCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHH
EERRLLYVAMTRAKDDLHLLVPQRFFVHGQPVRGDRHVYASRTRFIPEALVPLFERVSWP
HHHHEEEEEEECCCCCEEEECCCHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHCCCC
KLQAGVTVASAGPRIDLGARMRERWR
CHHCCEEEECCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MQAIAATYLDDLNPEQRRAVEHGGTSAAPGPPLLIIAGAGSGKTNTLAHRVAHLLVNGAD
CCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
PRRILLMTFSRRAASEMSRRVERIARKVLGGQAEAITAGLTWAGTFHGLGARMLREYAER
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEECHHHHHHHHHHHHHHHHH
IGLDPVFTIHDREDSADLMNLVRHERGLSNTQRRFPTKGTCLAIYSRCVNADMPLDEALV
CCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHH
RHFPWCAEWAGELKGLFAAYVEAKQANAVLDYDDLLLYWAQMMADETIAREIGERFDHVL
HHCCCHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDEYQDTNRLQSQILLGLKPDGAGLTVVGDDAQSIYAFRAATVRNILEFPDQFSPRADIV
HHHHHHHHHHHHHHEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
TLDRNYRSTQPILSAANAVIGLARERFTKNLWTDRTASRKPQLVTVRDETDQARYIVEQV
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHH
LAKREEGIVLKQQAVLFRTSSHSGPLEIELTRRNIPFVKFGGLKFLDAAHVKDMLACLRF
HHHHHCCEEEEEHEEEEEECCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHH
VENPRDRIAGFRVLHLIPGIGPASAQRLLDAISESADPLRTLCAQPTPARAGEDWTAFVR
HCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHH
TLEQLRSSDWPADIERIRHWYQPHLERVHEDAEMRSADLLQLEQIASGYASRERFLTELT
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LDPPDATSDQAGVPLLDEDYLILSTIHSAKGMEWNAVHVLNVVDGCMPSDLGAGSTAELE
CCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHH
EERRLLYVAMTRAKDDLHLLVPQRFFVHGQPVRGDRHVYASRTRFIPEALVPLFERVSWP
HHHHEEEEEEECCCCCEEEECCCHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHCCCC
KLQAGVTVASAGPRIDLGARMRERWR
CHHCCEEEECCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]