Click here to switch to the map view.

The map label for this gene is paaF [C]

Identifier: 146337774

GI number: 146337774

Start: 675596

End: 676402

Strand: Reverse

Name: paaF [C]

Synonym: BRADO0651

Alternate gene names: 146337774

Gene position: 676402-675596 (Counterclockwise)

Preceding gene: 146337775

Following gene: 146337771

Centisome position: 9.07

GC content: 66.67

Gene sequence:

>807_bases
ATGACTCACGTCCCTGACATCGGCGTCGAGATCCACGGCCATGTCGGCCTGATCGAGATCCGCCGGCCGCCGCTCAACTT
CTTCGACATTTCATTGATCAACCAGATCGCCGACGCGCTCGAAGGCTTCGATCGCGACATCGCGATCCGCGCCTCGGTGC
TTGCCGCGCAGGGCAAGGCGTTCTGCGCTGGCGCCAATTTCGGCGACCCGGCGCGGCAGGCGCAGGAGGCGGCCGCAGCG
AAAGGTGACCCGGCCGACAGCCTCGGGCCGATCAACCATCTCTATATGCAGGCGGTACGCATCTTCCGCTGCAAGAAGCC
TGTCGTCGCTGCCGTGCATGGCGCTGCGATTGGCGGCGGGCTGGGCTTGGCGGTGTCGGCTGACTTTCGCGTGACCTGTC
CGGAAGCGCGGTTCTCCGCCAACTTCACCAAGCTCGGCTTCCATCCGGGCTTCGGACTGACGGTGACGCTGCCGGAGCTG
GTCGGCAAGAACAACGCCGAGCTGATGTTCTACACCAGCCGCCGCGTCACCGGCGAGGAGGCCTACAAAATGGGCCTCGC
CAATGAATGCGTGCCGCAGGACCAGGTGCGCGACGCAGCGATGAAGCTCGCCGCCGAGATTGCCGAATGCTCGCCGCTCG
GCCTGCTCTCCACCCGCGCCACGATGCGCGCCGGCCTCGCCGACCGCGTCATGGCCGCCACCGAGCATGAGCTCGCCGAG
CAGACGCGGCTACGCGCGACGGAGGATTTCAAGGAGGGCGTGAAGGCCACGGAAGAGCGGCGCTTCGCGAACTTCAAGGG
GCGGTGA

Upstream 100 bases:

>100_bases
TCGCGAGCGCAGCGAAGCGATCCAGGGCCATTCGCACCAATCTGGATTGCTTCGCTGGGCTCGCGATGAGGTCGGAGAAG
CAGAATGGGAGACCACCCAC

Downstream 100 bases:

>100_bases
GTTTCCCTCGGATCGGTCACGAAGCTGGTATCGCATGGCACCGGGCAGCGGCGCATTCGGTAGACGTATGGACGAGGCCT
AGCGAGACTAGCTGTTTGGA

Product: putative enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MTHVPDIGVEIHGHVGLIEIRRPPLNFFDISLINQIADALEGFDRDIAIRASVLAAQGKAFCAGANFGDPARQAQEAAAA
KGDPADSLGPINHLYMQAVRIFRCKKPVVAAVHGAAIGGGLGLAVSADFRVTCPEARFSANFTKLGFHPGFGLTVTLPEL
VGKNNAELMFYTSRRVTGEEAYKMGLANECVPQDQVRDAAMKLAAEIAECSPLGLLSTRATMRAGLADRVMAATEHELAE
QTRLRATEDFKEGVKATEERRFANFKGR

Sequences:

>Translated_268_residues
MTHVPDIGVEIHGHVGLIEIRRPPLNFFDISLINQIADALEGFDRDIAIRASVLAAQGKAFCAGANFGDPARQAQEAAAA
KGDPADSLGPINHLYMQAVRIFRCKKPVVAAVHGAAIGGGLGLAVSADFRVTCPEARFSANFTKLGFHPGFGLTVTLPEL
VGKNNAELMFYTSRRVTGEEAYKMGLANECVPQDQVRDAAMKLAAEIAECSPLGLLSTRATMRAGLADRVMAATEHELAE
QTRLRATEDFKEGVKATEERRFANFKGR
>Mature_267_residues
THVPDIGVEIHGHVGLIEIRRPPLNFFDISLINQIADALEGFDRDIAIRASVLAAQGKAFCAGANFGDPARQAQEAAAAK
GDPADSLGPINHLYMQAVRIFRCKKPVVAAVHGAAIGGGLGLAVSADFRVTCPEARFSANFTKLGFHPGFGLTVTLPELV
GKNNAELMFYTSRRVTGEEAYKMGLANECVPQDQVRDAAMKLAAEIAECSPLGLLSTRATMRAGLADRVMAATEHELAEQ
TRLRATEDFKEGVKATEERRFANFKGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=264, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI70995211, Length=257, Percent_Identity=29.9610894941634, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI37594469, Length=195, Percent_Identity=32.3076923076923, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI37594471, Length=195, Percent_Identity=32.3076923076923, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI68989263, Length=189, Percent_Identity=31.7460317460317, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI62530384, Length=259, Percent_Identity=22.007722007722, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI25453481, Length=222, Percent_Identity=24.7747747747748, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI51538573, Length=222, Percent_Identity=24.7747747747748, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4757966, Length=222, Percent_Identity=24.7747747747748, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI51538581, Length=222, Percent_Identity=24.7747747747748, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4502327, Length=248, Percent_Identity=27.4193548387097, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757968, Length=222, Percent_Identity=24.3243243243243, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI49355787, Length=222, Percent_Identity=24.3243243243243, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI221136756, Length=193, Percent_Identity=25.9067357512953, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI221136753, Length=193, Percent_Identity=25.9067357512953, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI20127408, Length=194, Percent_Identity=28.8659793814433, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221307494, Length=193, Percent_Identity=25.9067357512953, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=266, Percent_Identity=30.8270676691729, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=30.4182509505703, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=28.7937743190661, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=28.7878787878788, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87082183, Length=248, Percent_Identity=24.5967741935484, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788682, Length=198, Percent_Identity=29.7979797979798, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790281, Length=239, Percent_Identity=24.6861924686192, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=226, Percent_Identity=30.9734513274336, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=267, Percent_Identity=31.0861423220974, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=28.9156626506024, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=220, Percent_Identity=26.3636363636364, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=213, Percent_Identity=26.2910798122066, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=241, Percent_Identity=24.896265560166, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=24.8888888888889, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=190, Percent_Identity=26.8421052631579, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=216, Percent_Identity=26.3888888888889, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI20129971, Length=263, Percent_Identity=31.9391634980989, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24653477, Length=263, Percent_Identity=31.9391634980989, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=30.5343511450382, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19920382, Length=248, Percent_Identity=28.6290322580645, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21357171, Length=255, Percent_Identity=27.4509803921569, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24650670, Length=268, Percent_Identity=29.8507462686567, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19922422, Length=269, Percent_Identity=26.3940520446097, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28821; Mature: 28690

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHVPDIGVEIHGHVGLIEIRRPPLNFFDISLINQIADALEGFDRDIAIRASVLAAQGKA
CCCCCCCCEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCE
FCAGANFGDPARQAQEAAAAKGDPADSLGPINHLYMQAVRIFRCKKPVVAAVHGAAIGGG
EEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
LGLAVSADFRVTCPEARFSANFTKLGFHPGFGLTVTLPELVGKNNAELMFYTSRRVTGEE
CEEEEECCEEEECCCHHHCCCCEEECCCCCCCEEEECHHHHCCCCCEEEEEECCCCCHHH
AYKMGLANECVPQDQVRDAAMKLAAEIAECSPLGLLSTRATMRAGLADRVMAATEHELAE
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHH
QTRLRATEDFKEGVKATEERRFANFKGR
HHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
THVPDIGVEIHGHVGLIEIRRPPLNFFDISLINQIADALEGFDRDIAIRASVLAAQGKA
CCCCCCCEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCE
FCAGANFGDPARQAQEAAAAKGDPADSLGPINHLYMQAVRIFRCKKPVVAAVHGAAIGGG
EEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
LGLAVSADFRVTCPEARFSANFTKLGFHPGFGLTVTLPELVGKNNAELMFYTSRRVTGEE
CEEEEECCEEEECCCHHHCCCCEEECCCCCCCEEEECHHHHCCCCCEEEEEECCCCCHHH
AYKMGLANECVPQDQVRDAAMKLAAEIAECSPLGLLSTRATMRAGLADRVMAATEHELAE
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHH
QTRLRATEDFKEGVKATEERRFANFKGR
HHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA