BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is paaG [C]

Identifier: 146337769

GI number: 146337769

Start: 669848

End: 670639

Strand: Direct

Name: paaG [C]

Synonym: BRADO0646

Alternate gene names: 146337769

Gene position: 669848-670639 (Clockwise)

Preceding gene: 146337768

Following gene: 146337770

Centisome position: 8.98

GC content: 68.18

Gene sequence:

>792_bases
ATGACCAACACCGCCGTCCTGCAGCACCTCGATGCCGGCCTGCTCACGATCACGATGAACCGCCCCGAGCGGCGCAATGC
GCTGAACAGCGACATGACCAAAGGCCTGGTCGAGGCGGCGCAGCGCGCGGCCAACGATCCGGACGTGCGCGCCGTGCTGC
TCAAGGGCGCCGGCGGCACGTTCTGCGTCGGCGGCGACGTCAAGGCGATGGCCGAGGCCAAGACGGCTCCGTCGTTCGAG
ACCAAGGCGACGACCTTGCGCGAGCGCATGGAGGTGTCCCGGCTCTTGCACCAGATGCGGAAGCCGGTCGTCGCGCAGAT
CGATGGCGCCGCGGCCGGCGCCGGACTGTCGATCGCACTCGCCTGCGATCTCCGCGTCGCCGGCGAATCCGCCAAGATCA
CGACGGCCTTCGCCAAGGTGGGACTGTCAGGCGATTTCGGCGGCACCTACTTCCTCACCCAGCTGCTCGGCAGCGCCAAG
GCGCGCGAGCTGTACCTGACCTCGCCGATCCTCTCCGCGCGCGAAGCCCTGGCGCTGGGCATGGTGACGAAGGTGGTGCC
CGACGCCGAGGTCGAGGCCGCCGCGCGCGAGCTCGCGATGTCGCTGGCTCAAGGCCCGAGCGTCACGCTCGGCTACATCA
AGCGCAACATCAACAATGCCGAGACGATGACCCTGGAAGCCTGCTTCGATGCCGAAGCCGTGCACCACTCGCGCTGCAGC
GACACCGAGGATCACAAGGAGGCTGCGCGAGCCTTCGTCGAGAAGCGCGTGCCCGTGTTCAAGGGGCATTAG

Upstream 100 bases:

>100_bases
GGAGCCTCGACATGAGTCCATCATCTCGGGATTCCGGGTTCGACGCTGACGCGTCGCCCCGGAATGACGGGTCCACAACT
CGGATATCGGAGCATCAGCC

Downstream 100 bases:

>100_bases
CACCACACTTTCCGTCATTGCGAGGAGCGAAGCGACGAAGCAATCCAGGGTGATTGTGTCAGCTCTGGATAGCTTCCGTC
TTCGCTCTGCGAGGTCCGGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTNTAVLQHLDAGLLTITMNRPERRNALNSDMTKGLVEAAQRAANDPDVRAVLLKGAGGTFCVGGDVKAMAEAKTAPSFE
TKATTLRERMEVSRLLHQMRKPVVAQIDGAAAGAGLSIALACDLRVAGESAKITTAFAKVGLSGDFGGTYFLTQLLGSAK
ARELYLTSPILSAREALALGMVTKVVPDAEVEAAARELAMSLAQGPSVTLGYIKRNINNAETMTLEACFDAEAVHHSRCS
DTEDHKEAARAFVEKRVPVFKGH

Sequences:

>Translated_263_residues
MTNTAVLQHLDAGLLTITMNRPERRNALNSDMTKGLVEAAQRAANDPDVRAVLLKGAGGTFCVGGDVKAMAEAKTAPSFE
TKATTLRERMEVSRLLHQMRKPVVAQIDGAAAGAGLSIALACDLRVAGESAKITTAFAKVGLSGDFGGTYFLTQLLGSAK
ARELYLTSPILSAREALALGMVTKVVPDAEVEAAARELAMSLAQGPSVTLGYIKRNINNAETMTLEACFDAEAVHHSRCS
DTEDHKEAARAFVEKRVPVFKGH
>Mature_262_residues
TNTAVLQHLDAGLLTITMNRPERRNALNSDMTKGLVEAAQRAANDPDVRAVLLKGAGGTFCVGGDVKAMAEAKTAPSFET
KATTLRERMEVSRLLHQMRKPVVAQIDGAAAGAGLSIALACDLRVAGESAKITTAFAKVGLSGDFGGTYFLTQLLGSAKA
RELYLTSPILSAREALALGMVTKVVPDAEVEAAARELAMSLAQGPSVTLGYIKRNINNAETMTLEACFDAEAVHHSRCSD
TEDHKEAARAFVEKRVPVFKGH

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=31.8367346938775, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI70995211, Length=186, Percent_Identity=32.7956989247312, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI37594471, Length=238, Percent_Identity=30.2521008403361, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI37594469, Length=238, Percent_Identity=30.2521008403361, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI4502327, Length=266, Percent_Identity=31.203007518797, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI31542718, Length=268, Percent_Identity=27.9850746268657, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI25453481, Length=184, Percent_Identity=32.6086956521739, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI51538573, Length=184, Percent_Identity=32.6086956521739, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI4757966, Length=184, Percent_Identity=32.6086956521739, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI51538581, Length=184, Percent_Identity=32.6086956521739, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI4757968, Length=184, Percent_Identity=32.0652173913043, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI49355787, Length=184, Percent_Identity=32.0652173913043, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI213417737, Length=231, Percent_Identity=27.2727272727273, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI157694516, Length=231, Percent_Identity=27.2727272727273, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI260274832, Length=218, Percent_Identity=27.5229357798165, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI45643119, Length=218, Percent_Identity=27.5229357798165, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI260275230, Length=218, Percent_Identity=27.5229357798165, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI68989263, Length=171, Percent_Identity=32.7485380116959, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI116235487, Length=218, Percent_Identity=27.5229357798165, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221307494, Length=162, Percent_Identity=32.0987654320988, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI221136756, Length=162, Percent_Identity=32.0987654320988, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI221136753, Length=162, Percent_Identity=32.0987654320988, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI157694520, Length=208, Percent_Identity=25.9615384615385, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI20127408, Length=219, Percent_Identity=25.5707762557078, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=38.5214007782101, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1787659, Length=250, Percent_Identity=30.8, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=31.6793893129771, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=28.8537549407115, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082183, Length=256, Percent_Identity=25.78125, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788682, Length=203, Percent_Identity=32.0197044334975, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790281, Length=184, Percent_Identity=27.1739130434783, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=30.7377049180328, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=30.4, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=219, Percent_Identity=33.7899543378995, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17560910, Length=209, Percent_Identity=28.7081339712919, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=246, Percent_Identity=27.2357723577236, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=254, Percent_Identity=28.3464566929134, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=187, Percent_Identity=31.0160427807487, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=187, Percent_Identity=31.0160427807487, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=218, Percent_Identity=27.0642201834862, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=211, Percent_Identity=27.9620853080569, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=165, Percent_Identity=32.1212121212121, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=189, Percent_Identity=25.9259259259259, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24653139, Length=274, Percent_Identity=31.7518248175182, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=31.9672131147541, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=31.9672131147541, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21357171, Length=257, Percent_Identity=32.295719844358, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24654903, Length=245, Percent_Identity=27.3469387755102, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24650670, Length=206, Percent_Identity=31.0679611650485, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=25.8964143426295, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571730, Length=142, Percent_Identity=33.0985915492958, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI28571729, Length=142, Percent_Identity=33.0985915492958, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19920382, Length=188, Percent_Identity=28.7234042553192, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27896; Mature: 27765

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTAVLQHLDAGLLTITMNRPERRNALNSDMTKGLVEAAQRAANDPDVRAVLLKGAGGT
CCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FCVGGDVKAMAEAKTAPSFETKATTLRERMEVSRLLHQMRKPVVAQIDGAAAGAGLSIAL
EEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEE
ACDLRVAGESAKITTAFAKVGLSGDFGGTYFLTQLLGSAKARELYLTSPILSAREALALG
EEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHCEEEECCHHHHHHHHHHHH
MVTKVVPDAEVEAAARELAMSLAQGPSVTLGYIKRNINNAETMTLEACFDAEAVHHSRCS
HHHHHCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCCCCEEEEHHHHCHHHHHHCCCC
DTEDHKEAARAFVEKRVPVFKGH
CCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TNTAVLQHLDAGLLTITMNRPERRNALNSDMTKGLVEAAQRAANDPDVRAVLLKGAGGT
CCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FCVGGDVKAMAEAKTAPSFETKATTLRERMEVSRLLHQMRKPVVAQIDGAAAGAGLSIAL
EEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEE
ACDLRVAGESAKITTAFAKVGLSGDFGGTYFLTQLLGSAKARELYLTSPILSAREALALG
EEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHCEEEECCHHHHHHHHHHHH
MVTKVVPDAEVEAAARELAMSLAQGPSVTLGYIKRNINNAETMTLEACFDAEAVHHSRCS
HHHHHCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCCCCEEEEHHHHCHHHHHHCCCC
DTEDHKEAARAFVEKRVPVFKGH
CCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA