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The map label for this gene is limB [H]

Identifier: 146337748

GI number: 146337748

Start: 646685

End: 647785

Strand: Direct

Name: limB [H]

Synonym: BRADO0622

Alternate gene names: 146337748

Gene position: 646685-647785 (Clockwise)

Preceding gene: 146337747

Following gene: 146337749

Centisome position: 8.67

GC content: 63.67

Gene sequence:

>1101_bases
ATGGAAATCGGATACTTCACGATGCCCTCGCATCCGCCGGAGTGCGGATTGAAGGAAGGGCAGGAGTGGGACCTTCAGGT
CCTGCGCTGGCTCGATGAGCTCGGCTATCAGGAAGCCTGGATCGGCGAGCATCACACCGCGCCATGGGAGCCGCATCCCG
CGCCCGATCTGATTTTGGCGCAGGCGTTCCGCGAGACCAAGAACATCCGCCTCGGCCCCGGCGGCTTCCTGCTGCCCTAT
CATCATCCGGCCGAGCTCGCCAACCGCGTCGCGATGCTGGATCATCTCTCCGACGGCCGGCTCAATTTCGGCATTGCCGC
ATCCGGCCTGCCGAGCGACTGGGCGATGTTCAATGTCGACGGCATTAGCGGCCAGAACCGCGAGATGACGCGCGAGGCGC
TGGAGATCATCCTGCGGCTATGGACCGAGCCGGCGCCGTTCACGCACAAGGGCAAGTACTGGACGGTGACCAAGCCTGAC
GTGATGTTTGACTTCCTGAAGCCGCATATCAAGCCGGTGCAGGCGCCGCATCCGCCGATCGGCGTCGCAGGTCTGTCGAA
GAACTCCGATACGCTCAAGCTCGCCGGCGAGCGCGGCTTCATTCCGATGAGTCTCAATCTCAACCCCGCCTATGTGTCGT
CGCACTGGGATTCGGTCGAGGCCGGCGCCGCCAAGTCCGGGCGCAAGCCGAGCCGCAACGATTGGCGGCTGGTCCGCGAG
GTGTTCGTCGCCGAGACCGATGAGGAGGCGTGGCGGCTCTCGACCGGCGACATGATGGGCCGGATGATGGGCGAGTACTT
CCTGCCGCTGCTGAGCCATTTCGGCTTCAAGGACTACCTCAAGCATTCGCCCGACGTGCCGGATACCGACGTGACGGTGG
ACTATTGCGCCAAGCGCAACTGGATCATCGGCTCGCCGGCGACGGTGACCGAGAAGATCGAGAAGATCTGGCACGAGGTC
GGCGGCTTCGGGACGCTGTGCGTGTTCGGCTTCGACTACAAGCACAAGCCGGAAGCCTGGCACAACTCGCTGCGCCTCCT
GAAGCACGAGGTGATGCCGAGGTTGCGCCATCTCAATGCCGATATGACCAGGGCGGCGTAA

Upstream 100 bases:

>100_bases
CACGTGCGGCGTTGCCGACTCCGTTGGTCGTGTTTCGTCTCGCGTGACGATGAGCGCATCACAAAGGTGCGCCGCAGTAT
AAACGCGAGAGGAAAGCGCC

Downstream 100 bases:

>100_bases
TATCAGCGATCGGGCCGGCGCAGGTGCGCCGGTCCGATCGACGAGGTCATTGCCATGCGCATTCGCGTCCGACAGTCAGC
CCATGTGCTCGAGCGGTCGG

Product: putative luciferase-like monooxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MEIGYFTMPSHPPECGLKEGQEWDLQVLRWLDELGYQEAWIGEHHTAPWEPHPAPDLILAQAFRETKNIRLGPGGFLLPY
HHPAELANRVAMLDHLSDGRLNFGIAASGLPSDWAMFNVDGISGQNREMTREALEIILRLWTEPAPFTHKGKYWTVTKPD
VMFDFLKPHIKPVQAPHPPIGVAGLSKNSDTLKLAGERGFIPMSLNLNPAYVSSHWDSVEAGAAKSGRKPSRNDWRLVRE
VFVAETDEEAWRLSTGDMMGRMMGEYFLPLLSHFGFKDYLKHSPDVPDTDVTVDYCAKRNWIIGSPATVTEKIEKIWHEV
GGFGTLCVFGFDYKHKPEAWHNSLRLLKHEVMPRLRHLNADMTRAA

Sequences:

>Translated_366_residues
MEIGYFTMPSHPPECGLKEGQEWDLQVLRWLDELGYQEAWIGEHHTAPWEPHPAPDLILAQAFRETKNIRLGPGGFLLPY
HHPAELANRVAMLDHLSDGRLNFGIAASGLPSDWAMFNVDGISGQNREMTREALEIILRLWTEPAPFTHKGKYWTVTKPD
VMFDFLKPHIKPVQAPHPPIGVAGLSKNSDTLKLAGERGFIPMSLNLNPAYVSSHWDSVEAGAAKSGRKPSRNDWRLVRE
VFVAETDEEAWRLSTGDMMGRMMGEYFLPLLSHFGFKDYLKHSPDVPDTDVTVDYCAKRNWIIGSPATVTEKIEKIWHEV
GGFGTLCVFGFDYKHKPEAWHNSLRLLKHEVMPRLRHLNADMTRAA
>Mature_366_residues
MEIGYFTMPSHPPECGLKEGQEWDLQVLRWLDELGYQEAWIGEHHTAPWEPHPAPDLILAQAFRETKNIRLGPGGFLLPY
HHPAELANRVAMLDHLSDGRLNFGIAASGLPSDWAMFNVDGISGQNREMTREALEIILRLWTEPAPFTHKGKYWTVTKPD
VMFDFLKPHIKPVQAPHPPIGVAGLSKNSDTLKLAGERGFIPMSLNLNPAYVSSHWDSVEAGAAKSGRKPSRNDWRLVRE
VFVAETDEEAWRLSTGDMMGRMMGEYFLPLLSHFGFKDYLKHSPDVPDTDVTVDYCAKRNWIIGSPATVTEKIEKIWHEV
GGFGTLCVFGFDYKHKPEAWHNSLRLLKHEVMPRLRHLNADMTRAA

Specific function: Acts on both enantiomers of limonene by their NAD- dependent epoxidation at the 1,2 double bond forming limonene-1,2- epoxide [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial luciferase oxidoreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR016048 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: =1.14.13.107 [H]

Molecular weight: Translated: 41522; Mature: 41522

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIGYFTMPSHPPECGLKEGQEWDLQVLRWLDELGYQEAWIGEHHTAPWEPHPAPDLILA
CCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHH
QAFRETKNIRLGPGGFLLPYHHPAELANRVAMLDHLSDGRLNFGIAASGLPSDWAMFNVD
HHHHHHCCEEECCCCEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEC
GISGQNREMTREALEIILRLWTEPAPFTHKGKYWTVTKPDVMFDFLKPHIKPVQAPHPPI
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCC
GVAGLSKNSDTLKLAGERGFIPMSLNLNPAYVSSHWDSVEAGAAKSGRKPSRNDWRLVRE
EEEECCCCCCEEEECCCCCEEEEEECCCHHHHHCCCCCHHCCCCCCCCCCCCHHHHHHHH
VFVAETDEEAWRLSTGDMMGRMMGEYFLPLLSHFGFKDYLKHSPDVPDTDVTVDYCAKRN
HHHHCCCCHHHEECHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHCCCC
WIIGSPATVTEKIEKIWHEVGGFGTLCVFGFDYKHKPEAWHNSLRLLKHEVMPRLRHLNA
EEECCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DMTRAA
HHHHCC
>Mature Secondary Structure
MEIGYFTMPSHPPECGLKEGQEWDLQVLRWLDELGYQEAWIGEHHTAPWEPHPAPDLILA
CCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHH
QAFRETKNIRLGPGGFLLPYHHPAELANRVAMLDHLSDGRLNFGIAASGLPSDWAMFNVD
HHHHHHCCEEECCCCEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEC
GISGQNREMTREALEIILRLWTEPAPFTHKGKYWTVTKPDVMFDFLKPHIKPVQAPHPPI
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCC
GVAGLSKNSDTLKLAGERGFIPMSLNLNPAYVSSHWDSVEAGAAKSGRKPSRNDWRLVRE
EEEECCCCCCEEEECCCCCEEEEEECCCHHHHHCCCCCHHCCCCCCCCCCCCHHHHHHHH
VFVAETDEEAWRLSTGDMMGRMMGEYFLPLLSHFGFKDYLKHSPDVPDTDVTVDYCAKRN
HHHHCCCCHHHEECHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHCCCC
WIIGSPATVTEKIEKIWHEVGGFGTLCVFGFDYKHKPEAWHNSLRLLKHEVMPRLRHLNA
EEECCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DMTRAA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA