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The map label for this gene is yafV [C]

Identifier: 146337712

GI number: 146337712

Start: 596354

End: 597232

Strand: Reverse

Name: yafV [C]

Synonym: BRADO0581

Alternate gene names: 146337712

Gene position: 597232-596354 (Counterclockwise)

Preceding gene: 146337713

Following gene: 146337711

Centisome position: 8.01

GC content: 67.01

Gene sequence:

>879_bases
ATGAGCAACGACCGCAGCTTTACCGCCGCCATGGTGCAGATGCGCACCGCGCTGTTGCCCGAGCCGAGCCTCGAGCAGGG
CACCAGGCTGATCCGGGAAGCGGTCGCGCAGGGCGCGCAATACGTCCAGACGCCGGAAGTGAGCAACATGATGCAGCTCA
ACCGGACGGCGCTGTTCGAGCAGCTCAAGAGCGAGGAGGAAGATCCCTCGCTGAAGGCCTATCGCGCGCTCGCCAAGGAG
CTGAACATCCATCTGCACATCGGATCGCTGGCGCTGCGCTTCTCGGCCGAGAAGGCCGTGAACCGCTCGTTCCTGATCGG
GCCCGACGGCCAGGTGCTGGCGAGCTACGACAAGATCCACATGTTCGACATCGACCTGCCGGGCGGTGAGAGCTATCGCG
AGTCCGCGAACTATCAGCCGGGCGAAACGGCGGTGATCTCGGATCTGCCGTGGGGCCGGCTGGGCCTGACGATCTGCTAC
GACGTCCGCTTTCCGGCGCTGTATCGCGCGCTCGCCGAGAGCGGCGCGTCGTTCATCAGCGTGCCCTCGGCGTTCACCCG
CAAGACCGGCGAGGCCCACTGGCACACCTTGCTGCGCGCCCGCGCCATCGAGACCGGCTGCTTCGTGTTCGCCGCCGCGC
AATGCGGCCTGCACGAGAACAAGCGCGAGACCTTCGGCCATTCGCTGATCATCGATCCCTGGGGCGAGATCCTCGCCGAG
GGCGGCGTCGAGCCCGGCGTCATCCTGGCGCGCATCGATCCCTCCAGGGTCGAGAGCGTGCGCCAGACCATTCCTTCCCT
GCAGCACGGCCGCCGGTTCGGTATCGCCGATCCCAAGGGCGGACCGGACTATCTGCACCTCGTGCGGGGCTCGGCATGA

Upstream 100 bases:

>100_bases
TCTTCATCGGCAAGACCCATATCGGCGGCTGCGACGATCTCTATGCGCTCGATCGCGAGGGCAGGCTCGACGCCATGCTG
GCTGGAGACAAGGCGACATC

Downstream 100 bases:

>100_bases
TCCGCTATGCGCTGCGCTGCGAGCGTGATCATTCGTTCGAAAGCTGGTTTCAGAGCTCCTCGGCCTACGACTCTCAGGTG
AAGCGCAAGCTGGTGGAATG

Product: putative nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSNDRSFTAAMVQMRTALLPEPSLEQGTRLIREAVAQGAQYVQTPEVSNMMQLNRTALFEQLKSEEEDPSLKAYRALAKE
LNIHLHIGSLALRFSAEKAVNRSFLIGPDGQVLASYDKIHMFDIDLPGGESYRESANYQPGETAVISDLPWGRLGLTICY
DVRFPALYRALAESGASFISVPSAFTRKTGEAHWHTLLRARAIETGCFVFAAAQCGLHENKRETFGHSLIIDPWGEILAE
GGVEPGVILARIDPSRVESVRQTIPSLQHGRRFGIADPKGGPDYLHLVRGSA

Sequences:

>Translated_292_residues
MSNDRSFTAAMVQMRTALLPEPSLEQGTRLIREAVAQGAQYVQTPEVSNMMQLNRTALFEQLKSEEEDPSLKAYRALAKE
LNIHLHIGSLALRFSAEKAVNRSFLIGPDGQVLASYDKIHMFDIDLPGGESYRESANYQPGETAVISDLPWGRLGLTICY
DVRFPALYRALAESGASFISVPSAFTRKTGEAHWHTLLRARAIETGCFVFAAAQCGLHENKRETFGHSLIIDPWGEILAE
GGVEPGVILARIDPSRVESVRQTIPSLQHGRRFGIADPKGGPDYLHLVRGSA
>Mature_291_residues
SNDRSFTAAMVQMRTALLPEPSLEQGTRLIREAVAQGAQYVQTPEVSNMMQLNRTALFEQLKSEEEDPSLKAYRALAKEL
NIHLHIGSLALRFSAEKAVNRSFLIGPDGQVLASYDKIHMFDIDLPGGESYRESANYQPGETAVISDLPWGRLGLTICYD
VRFPALYRALAESGASFISVPSAFTRKTGEAHWHTLLRARAIETGCFVFAAAQCGLHENKRETFGHSLIIDPWGEILAEG
GVEPGVILARIDPSRVESVRQTIPSLQHGRRFGIADPKGGPDYLHLVRGSA

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632350, Length=269, Percent_Identity=37.9182156133829, Blast_Score=181, Evalue=7e-46,
Organism=Homo sapiens, GI297632348, Length=269, Percent_Identity=37.9182156133829, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI5031947, Length=269, Percent_Identity=37.9182156133829, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI9910460, Length=266, Percent_Identity=34.9624060150376, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI297632346, Length=192, Percent_Identity=32.2916666666667, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786412, Length=211, Percent_Identity=29.8578199052133, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556280, Length=274, Percent_Identity=38.3211678832117, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6322335, Length=296, Percent_Identity=33.445945945946, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323383, Length=217, Percent_Identity=35.9447004608295, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17933642, Length=274, Percent_Identity=40.5109489051095, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21355835, Length=195, Percent_Identity=30.2564102564103, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 32161; Mature: 32030

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDRSFTAAMVQMRTALLPEPSLEQGTRLIREAVAQGAQYVQTPEVSNMMQLNRTALFE
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHH
QLKSEEEDPSLKAYRALAKELNIHLHIGSLALRFSAEKAVNRSFLIGPDGQVLASYDKIH
HHHCCCCCCHHHHHHHHHHHCEEEEEECCEEEEECHHHHCCCEEEECCCCCEEEECCEEE
MFDIDLPGGESYRESANYQPGETAVISDLPWGRLGLTICYDVRFPALYRALAESGASFIS
EEEEECCCCCHHHHCCCCCCCCEEEECCCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEE
VPSAFTRKTGEAHWHTLLRARAIETGCFVFAAAQCGLHENKRETFGHSLIIDPWGEILAE
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEECCHHHHHHH
GGVEPGVILARIDPSRVESVRQTIPSLQHGRRFGIADPKGGPDYLHLVRGSA
CCCCCCEEEEECCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
SNDRSFTAAMVQMRTALLPEPSLEQGTRLIREAVAQGAQYVQTPEVSNMMQLNRTALFE
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHH
QLKSEEEDPSLKAYRALAKELNIHLHIGSLALRFSAEKAVNRSFLIGPDGQVLASYDKIH
HHHCCCCCCHHHHHHHHHHHCEEEEEECCEEEEECHHHHCCCEEEECCCCCEEEECCEEE
MFDIDLPGGESYRESANYQPGETAVISDLPWGRLGLTICYDVRFPALYRALAESGASFIS
EEEEECCCCCHHHHCCCCCCCCEEEECCCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEE
VPSAFTRKTGEAHWHTLLRARAIETGCFVFAAAQCGLHENKRETFGHSLIIDPWGEILAE
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEECCHHHHHHH
GGVEPGVILARIDPSRVESVRQTIPSLQHGRRFGIADPKGGPDYLHLVRGSA
CCCCCCEEEEECCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]