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The map label for this gene is gloB [H]

Identifier: 146337699

GI number: 146337699

Start: 583041

End: 583808

Strand: Reverse

Name: gloB [H]

Synonym: BRADO0567

Alternate gene names: 146337699

Gene position: 583808-583041 (Counterclockwise)

Preceding gene: 146337701

Following gene: 146337698

Centisome position: 7.83

GC content: 65.36

Gene sequence:

>768_bases
ATGGCCGCCGACATCCGCGTCTTCACCTGCCTGTCCGACAATTTCGGCTATCTGATCCACGATCCCGCAACTGGCGCCAC
GGCCTCGGTCGACGCCCCCGAGGCGGGTCCGATCGTCCGGCAGCTGGAGGCCAACGGCTGGACGCTGACCGACATTCTGA
TCACCCATCATCACTACGATCATGTCGGCGGCGTTGCCGAGTTGAAGCAGCGCTACGGCTGCCGCGTGATCGGGCCTCAT
GACAGGAAGGCCGCCATCGCGGATGTCGACCTGCGCGTCGCCCATGGCGATGTCGTCAAGGTCGGCGAGCTCCTGGCGCG
CGTGCTGGAGACGCCCGGCCACACGCTCGACCATGTGTCCTACGTGTTCGATGCCGACAAGGCGGTGTTCGCGGCCGATA
CGCTGTTCTCGATCGGCTGCGGCCGCGTCTTCGAGGGAACCTATCCGATGATGTGGGATTCGCTGCTGAAGCTGCGAACC
CTGCCCGACGATTTCAGGCTGTATTGCGGCCACGAATACACGGCGTCGAACGTCAAGTTCGCGCTCACCGTCGATCCGGA
CAATGAGGCGCTGAAGGCGCGTGCCGCCGAGGTGACGCGGCTGCGCGCGGCGAATCAGCCCACCATCCCCGTGCTGCTCG
GCGAGGAGAAGCAAGCCAACGTGTTTCTGCGCGCCGATGACCCGGCGATCGCCGTGCGGCTCCGCATGAAGGGAGCGAGC
GCCGAGGAGGTGTTCGGCGAGCTGCGCGAGCGCAAGAACAAGTCGTAA

Upstream 100 bases:

>100_bases
AGGCCGCCCGCGACGCTGCGCCCCGGGCCCGGTCTCTCAGCCAGATCTGACATCACGCGCAGCGCGTTCGTGCCGCGCCG
GTACCGCCGACGGAGACATC

Downstream 100 bases:

>100_bases
GCCCGCGGATCACGATCATGACCACGCTCCTCGCCGCCGACATCATCGCCCGTCTCGGCCTGCAGCCGCATCCGGAGGGT
GGTCACTTCCGCGAGACGTT

Product: putative hydroxyacylglutathione hydrolase (glyoxalase II)

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MAADIRVFTCLSDNFGYLIHDPATGATASVDAPEAGPIVRQLEANGWTLTDILITHHHYDHVGGVAELKQRYGCRVIGPH
DRKAAIADVDLRVAHGDVVKVGELLARVLETPGHTLDHVSYVFDADKAVFAADTLFSIGCGRVFEGTYPMMWDSLLKLRT
LPDDFRLYCGHEYTASNVKFALTVDPDNEALKARAAEVTRLRAANQPTIPVLLGEEKQANVFLRADDPAIAVRLRMKGAS
AEEVFGELRERKNKS

Sequences:

>Translated_255_residues
MAADIRVFTCLSDNFGYLIHDPATGATASVDAPEAGPIVRQLEANGWTLTDILITHHHYDHVGGVAELKQRYGCRVIGPH
DRKAAIADVDLRVAHGDVVKVGELLARVLETPGHTLDHVSYVFDADKAVFAADTLFSIGCGRVFEGTYPMMWDSLLKLRT
LPDDFRLYCGHEYTASNVKFALTVDPDNEALKARAAEVTRLRAANQPTIPVLLGEEKQANVFLRADDPAIAVRLRMKGAS
AEEVFGELRERKNKS
>Mature_254_residues
AADIRVFTCLSDNFGYLIHDPATGATASVDAPEAGPIVRQLEANGWTLTDILITHHHYDHVGGVAELKQRYGCRVIGPHD
RKAAIADVDLRVAHGDVVKVGELLARVLETPGHTLDHVSYVFDADKAVFAADTLFSIGCGRVFEGTYPMMWDSLLKLRTL
PDDFRLYCGHEYTASNVKFALTVDPDNEALKARAAEVTRLRAANQPTIPVLLGEEKQANVFLRADDPAIAVRLRMKGASA
EEVFGELRERKNKS

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538322, Length=233, Percent_Identity=38.1974248927039, Blast_Score=154, Evalue=6e-38,
Organism=Homo sapiens, GI94538320, Length=233, Percent_Identity=38.1974248927039, Blast_Score=154, Evalue=7e-38,
Organism=Homo sapiens, GI14150041, Length=231, Percent_Identity=37.2294372294372, Blast_Score=150, Evalue=8e-37,
Organism=Homo sapiens, GI116642887, Length=221, Percent_Identity=41.1764705882353, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI21703352, Length=221, Percent_Identity=41.1764705882353, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI46361987, Length=197, Percent_Identity=37.0558375634518, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI41327741, Length=193, Percent_Identity=28.4974093264249, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786406, Length=253, Percent_Identity=39.9209486166008, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1787158, Length=159, Percent_Identity=32.7044025157233, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17536925, Length=255, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17538952, Length=209, Percent_Identity=26.7942583732057, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320478, Length=267, Percent_Identity=30.3370786516854, Blast_Score=92, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6324614, Length=263, Percent_Identity=27.7566539923954, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356335, Length=260, Percent_Identity=37.3076923076923, Blast_Score=170, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24667711, Length=260, Percent_Identity=37.3076923076923, Blast_Score=170, Evalue=8e-43,
Organism=Drosophila melanogaster, GI24667703, Length=260, Percent_Identity=37.3076923076923, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI221330176, Length=178, Percent_Identity=30.3370786516854, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28023; Mature: 27892

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADIRVFTCLSDNFGYLIHDPATGATASVDAPEAGPIVRQLEANGWTLTDILITHHHYD
CCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHEEECCCCEEEEEEEEECCCCH
HVGGVAELKQRYGCRVIGPHDRKAAIADVDLRVAHGDVVKVGELLARVLETPGHTLDHVS
HHCCHHHHHHHCCCEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHH
YVFDADKAVFAADTLFSIGCGRVFEGTYPMMWDSLLKLRTLPDDFRLYCGHEYTASNVKF
HEECCCCCEEEHHHHHHCCCCCEECCCCHHHHHHHHHHHCCCCHHEEEECCEEECCCEEE
ALTVDPDNEALKARAAEVTRLRAANQPTIPVLLGEEKQANVFLRADDPAIAVRLRMKGAS
EEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCCEEEEEEEECCCC
AEEVFGELRERKNKS
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
AADIRVFTCLSDNFGYLIHDPATGATASVDAPEAGPIVRQLEANGWTLTDILITHHHYD
CCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHEEECCCCEEEEEEEEECCCCH
HVGGVAELKQRYGCRVIGPHDRKAAIADVDLRVAHGDVVKVGELLARVLETPGHTLDHVS
HHCCHHHHHHHCCCEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHH
YVFDADKAVFAADTLFSIGCGRVFEGTYPMMWDSLLKLRTLPDDFRLYCGHEYTASNVKF
HEECCCCCEEEHHHHHHCCCCCEECCCCHHHHHHHHHHHCCCCHHEEEECCEEECCCEEE
ALTVDPDNEALKARAAEVTRLRAANQPTIPVLLGEEKQANVFLRADDPAIAVRLRMKGAS
EEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCCEEEEEEEECCCC
AEEVFGELRERKNKS
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA