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The map label for this gene is 146337653

Identifier: 146337653

GI number: 146337653

Start: 525205

End: 526533

Strand: Direct

Name: 146337653

Synonym: BRADO0519

Alternate gene names: NA

Gene position: 525205-526533 (Clockwise)

Preceding gene: 146337652

Following gene: 146337654

Centisome position: 7.04

GC content: 63.36

Gene sequence:

>1329_bases
ATGCCGATAGGTGAGTTTGGCGGTGCACCGCCCCTGGCGTCGGAAGGCAGTCCGGTCCTGACGACGCCGATGTACTGGAT
GTACGAAATGGCGCATGCCTCGCTCAATCCGGCGCGCGCTGTCACCGATGCCACCAAGATCCTGTTTCAGAACCCGCTCA
ATCCCTGGGCGCACACCGAGATGGGCAAGTCGATCGCAGCGGCCTGCGAGCTGTTCGAGCGCACCACGCGCCGCTACGGC
AAGCCCGAATGGGGACTCGACGAGACCGAGGTGAACGGCATCAGAACCGCGGTCGAGGTCCGCTCGATCTGGGAAAAGCC
GTTCTGCCGCCTGCTCTATTTCGACCGCAAGCTGACCCGTCCGCTCCGCGCACCGCAGCCGCGGCTGCTGATCGTGGCGC
CGATGTCCGGCCATTATGCCACCCTGCTGCGTGGCACGGTCGAGGCGTTCCTGCCGACGCATGAGGTCTACATCACCGAC
TGGTCGGACGCGCGCATGGTGCCGCTGGCCGAAGGTCGCTTCGATCTCGACGACTACATCGATTACGTCATCGAGATGCT
GCGCTTCCTCGGCACCAACATGCACGTCATGGCGGTGTGCCAGCCGGCGGTGCCGGTGCTGGCCGCGGTGTCCGTCATGG
AGGCCGAGCGCGATCCATTCGCACCGATCTCGATGACGCTGATGGGTGGGCCGATCGACACGCGTCGCAATCCGACCGGC
GTGAACAATCTCGCCCAGGAGCGCGGCATCGACTGGTTCCGCAACCACGTCATCACCAAGGTGCCGTTCCCGCATCCCGG
CGTGATGCGCGACGTCTATCCGGGCTTCCTGCAGCTGAACGGGTTCATCAGCATGAACCTGGACCGGCACGTCGACGCCC
ACAAGGCGCTGTTCGCCAACCTGGTGAAGGGCGACGGCGACCAAGTAGACAAGCATCGCGACTTCTACGACGAGTATCTC
GCCGTGATGGACCTGGCTGCGGAATATTATTTGCAGACAGTGGAGACGGTGTTCATCCGCCACGCGCTGCCGAAGGGCGA
GATGACCCATCGCGGGGTGCTGGTCGATCCGTCGAAGGTCACGCGTGTGGCGCTGATGACGGTCGAAGGCGAGAAGGACG
ACATCTCCGGTCTTGGTCAGACCGAAGCGACGCATGGTCTGTGCTCGTCGATCCCGGATCATCGCCGGGTTCACTACGTA
CAGCCCGGTGTCGGACATTACGGCGTGTTCAACGGCTCGCGATTCAAGTCGGAGATCGTGCCACGCATCAACGACTTCAT
GCTGTCGGCGGCCGATCCGAAGTCGCTGCTCGCGATTGCTGCTGAATAG

Upstream 100 bases:

>100_bases
GCTCATTTCACTGCTTTCAGGCACCAACTCGCAACCCAGCGCATTGACGCTTAATTACGCATTCGGCAGTATGCTGCGTT
GCAGAGAGGGTTGAACGAGA

Downstream 100 bases:

>100_bases
CGTCGAGGGCGAGGTTTCGCGCAGTCGAATCCGACTGCGCGTTAACTCTTTGAAGAGGCTTCGAAAACCTGAATTTTGGG
GCGGCTCAAGCAGTGAATGT

Product: putative intracellular PHB depolymerase

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; PHB De-Polymerase Domain-Containing Protein; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Poly(3-Hydroxyalkanoate)Synthetase; Alpha/Beta Hydrolase Fold; Alpha/Beta Hydrolase Fold Protein; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; PHB De-Polymerase; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MPIGEFGGAPPLASEGSPVLTTPMYWMYEMAHASLNPARAVTDATKILFQNPLNPWAHTEMGKSIAAACELFERTTRRYG
KPEWGLDETEVNGIRTAVEVRSIWEKPFCRLLYFDRKLTRPLRAPQPRLLIVAPMSGHYATLLRGTVEAFLPTHEVYITD
WSDARMVPLAEGRFDLDDYIDYVIEMLRFLGTNMHVMAVCQPAVPVLAAVSVMEAERDPFAPISMTLMGGPIDTRRNPTG
VNNLAQERGIDWFRNHVITKVPFPHPGVMRDVYPGFLQLNGFISMNLDRHVDAHKALFANLVKGDGDQVDKHRDFYDEYL
AVMDLAAEYYLQTVETVFIRHALPKGEMTHRGVLVDPSKVTRVALMTVEGEKDDISGLGQTEATHGLCSSIPDHRRVHYV
QPGVGHYGVFNGSRFKSEIVPRINDFMLSAADPKSLLAIAAE

Sequences:

>Translated_442_residues
MPIGEFGGAPPLASEGSPVLTTPMYWMYEMAHASLNPARAVTDATKILFQNPLNPWAHTEMGKSIAAACELFERTTRRYG
KPEWGLDETEVNGIRTAVEVRSIWEKPFCRLLYFDRKLTRPLRAPQPRLLIVAPMSGHYATLLRGTVEAFLPTHEVYITD
WSDARMVPLAEGRFDLDDYIDYVIEMLRFLGTNMHVMAVCQPAVPVLAAVSVMEAERDPFAPISMTLMGGPIDTRRNPTG
VNNLAQERGIDWFRNHVITKVPFPHPGVMRDVYPGFLQLNGFISMNLDRHVDAHKALFANLVKGDGDQVDKHRDFYDEYL
AVMDLAAEYYLQTVETVFIRHALPKGEMTHRGVLVDPSKVTRVALMTVEGEKDDISGLGQTEATHGLCSSIPDHRRVHYV
QPGVGHYGVFNGSRFKSEIVPRINDFMLSAADPKSLLAIAAE
>Mature_441_residues
PIGEFGGAPPLASEGSPVLTTPMYWMYEMAHASLNPARAVTDATKILFQNPLNPWAHTEMGKSIAAACELFERTTRRYGK
PEWGLDETEVNGIRTAVEVRSIWEKPFCRLLYFDRKLTRPLRAPQPRLLIVAPMSGHYATLLRGTVEAFLPTHEVYITDW
SDARMVPLAEGRFDLDDYIDYVIEMLRFLGTNMHVMAVCQPAVPVLAAVSVMEAERDPFAPISMTLMGGPIDTRRNPTGV
NNLAQERGIDWFRNHVITKVPFPHPGVMRDVYPGFLQLNGFISMNLDRHVDAHKALFANLVKGDGDQVDKHRDFYDEYLA
VMDLAAEYYLQTVETVFIRHALPKGEMTHRGVLVDPSKVTRVALMTVEGEKDDISGLGQTEATHGLCSSIPDHRRVHYVQ
PGVGHYGVFNGSRFKSEIVPRINDFMLSAADPKSLLAIAAE

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 49492; Mature: 49361

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIGEFGGAPPLASEGSPVLTTPMYWMYEMAHASLNPARAVTDATKILFQNPLNPWAHTE
CCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHH
MGKSIAAACELFERTTRRYGKPEWGLDETEVNGIRTAVEVRSIWEKPFCRLLYFDRKLTR
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLRAPQPRLLIVAPMSGHYATLLRGTVEAFLPTHEVYITDWSDARMVPLAEGRFDLDDYI
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCHHHHH
DYVIEMLRFLGTNMHVMAVCQPAVPVLAAVSVMEAERDPFAPISMTLMGGPIDTRRNPTG
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
VNNLAQERGIDWFRNHVITKVPFPHPGVMRDVYPGFLQLNGFISMNLDRHVDAHKALFAN
HHHHHHHHCCHHHHHCCEEECCCCCCCCHHHHCCCCEEECCEEEECCHHCCHHHHHHHHH
LVKGDGDQVDKHRDFYDEYLAVMDLAAEYYLQTVETVFIRHALPKGEMTHRGVLVDPSKV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHE
TRVALMTVEGEKDDISGLGQTEATHGLCSSIPDHRRVHYVQPGVGHYGVFNGSRFKSEIV
EEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHH
PRINDFMLSAADPKSLLAIAAE
HHHHHHHCCCCCCCCEEEEECC
>Mature Secondary Structure 
PIGEFGGAPPLASEGSPVLTTPMYWMYEMAHASLNPARAVTDATKILFQNPLNPWAHTE
CCCCCCCCCCCCCCCCCEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHH
MGKSIAAACELFERTTRRYGKPEWGLDETEVNGIRTAVEVRSIWEKPFCRLLYFDRKLTR
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLRAPQPRLLIVAPMSGHYATLLRGTVEAFLPTHEVYITDWSDARMVPLAEGRFDLDDYI
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCHHHHH
DYVIEMLRFLGTNMHVMAVCQPAVPVLAAVSVMEAERDPFAPISMTLMGGPIDTRRNPTG
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
VNNLAQERGIDWFRNHVITKVPFPHPGVMRDVYPGFLQLNGFISMNLDRHVDAHKALFAN
HHHHHHHHCCHHHHHCCEEECCCCCCCCHHHHCCCCEEECCEEEECCHHCCHHHHHHHHH
LVKGDGDQVDKHRDFYDEYLAVMDLAAEYYLQTVETVFIRHALPKGEMTHRGVLVDPSKV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHE
TRVALMTVEGEKDDISGLGQTEATHGLCSSIPDHRRVHYVQPGVGHYGVFNGSRFKSEIV
EEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHH
PRINDFMLSAADPKSLLAIAAE
HHHHHHHCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA