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The map label for this gene is sucB [H]

Identifier: 146337553

GI number: 146337553

Start: 416144

End: 417385

Strand: Direct

Name: sucB [H]

Synonym: BRADO0407

Alternate gene names: 146337553

Gene position: 416144-417385 (Clockwise)

Preceding gene: 146337552

Following gene: 146337554

Centisome position: 5.58

GC content: 68.28

Gene sequence:

>1242_bases
ATGACTGACATTCGCGTTCCGACGCTCGGTGAATCCGTCACCGAAGCCACCATCGGCCGCTGGTTCAAGAAGGCGGGCGA
CGCGGTCGCCGTCGACGAGCCGCTGGTCGAGCTCGAGACCGACAAGGTGACCATCGAGGTGCCCGCCCCGTCGGCCGGCA
CGCTCGGCGAGATCATCGCCAAGGACGGTGAGACCGTCGCCGTCGGCGCGCTGCTCGGCCAGATCAATGACGGCGCGGCC
GCTGCCAAGCCGGCCGCCGCTGCGCCCGCGCCGGCTCCGGCAAAGCCCGCCGCGGCGGCTCCGGCTCCCGCGCCGGCGCC
GGCCAAGGCGCTGCCGGCCGATACGCCGCAGGCCCCGTCGGTGCGCAAGCTCTCGGCCGAGAGCGGTGTCGATGCCACCA
CGGTGCCGGGCTCCGGCAAGGATGGCCGCGTCACCAAGGGCGACATGCTGGCCGCGATCGAGCGCGCCGCGTCGGCGCCG
ACCCCGGTCAATCAGCCCGCCGCATCCGTGCAGGTCCGCGCGCCGTCGCCTGCCGATGACGCCGCGCGCGAGGAGCGCGT
CAAGATGACCCGGCTGCGCCAGACCATCGCGCGCCGTCTCAAGGACGTGCAGAACACCGCGGCGATGCTGACGACCTTCA
ACGAGGTCGACATGACCCACGTGATGGCGCTGCGCAGCCAGTACAAGGACGTGTTCGAGAAGAAGCACGGCTCCAAGCTC
GGCTTCATGGGCTTCTTCACCAAGGCCGTGGTGCAGGCGCTGAAGGACATCCCGGCGGTCAACGCCGAGATCGACGGCAG
CGACCTGATCTACAAGAACTACTATCATATCGGCGTCGCCGTCGGCACCGACAAGGGCCTGGTCGTGCCGGTCGTGCGCG
ACTGCGACCACAAGTCGATCGCCGACATCGAGAAGGGCATCGCCGATTTCGGCCGCCGCGCCCGTGACGGCCAGCTCAAG
ATCGACGAGATGCAGGGCGGCACCTTCACGATCACCAATGGCGGCATCTACGGCTCGCTGATGTCGACGCCGATCCTGAA
CGCGCCGCAGTCCGGCATCCTCGGCATGCACAAGATCCAGGAGCGTCCGATGGTGGTCGGCGGCAAGATCGAGGTGCGCC
CGATGATGTATCTGGCGCTGTCCTATGATCACCGCGTCATCGACGGCAAGGAAGCCGTCACCTTCCTGGTGCGCGTCAAG
GAAAGCCTGGAAGATCCGGCGCGGCTCGTCCTGGATCTCTGA

Upstream 100 bases:

>100_bases
AACAGTCCATCGCGTTGATCGTCCGTACCGCATCGCTTGCTCGCCGCAAGGCGATGGCGGACGGGCTTTATGACCGCACG
GGTTATTGAGGATAGAGACC

Downstream 100 bases:

>100_bases
GCCGAACGACGCCTTGACGACACGAGGCGCGCTTCCCAAGAGCGCGCCTCAGACCTAACGGGGCCATCATGACCGACAGG
GTTGCAGTGATCACCGGCGG

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTDIRVPTLGESVTEATIGRWFKKAGDAVAVDEPLVELETDKVTIEVPAPSAGTLGEIIAKDGETVAVGALLGQINDGAA
AAKPAAAAPAPAPAKPAAAAPAPAPAPAKALPADTPQAPSVRKLSAESGVDATTVPGSGKDGRVTKGDMLAAIERAASAP
TPVNQPAASVQVRAPSPADDAAREERVKMTRLRQTIARRLKDVQNTAAMLTTFNEVDMTHVMALRSQYKDVFEKKHGSKL
GFMGFFTKAVVQALKDIPAVNAEIDGSDLIYKNYYHIGVAVGTDKGLVVPVVRDCDHKSIADIEKGIADFGRRARDGQLK
IDEMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHKIQERPMVVGGKIEVRPMMYLALSYDHRVIDGKEAVTFLVRVK
ESLEDPARLVLDL

Sequences:

>Translated_413_residues
MTDIRVPTLGESVTEATIGRWFKKAGDAVAVDEPLVELETDKVTIEVPAPSAGTLGEIIAKDGETVAVGALLGQINDGAA
AAKPAAAAPAPAPAKPAAAAPAPAPAPAKALPADTPQAPSVRKLSAESGVDATTVPGSGKDGRVTKGDMLAAIERAASAP
TPVNQPAASVQVRAPSPADDAAREERVKMTRLRQTIARRLKDVQNTAAMLTTFNEVDMTHVMALRSQYKDVFEKKHGSKL
GFMGFFTKAVVQALKDIPAVNAEIDGSDLIYKNYYHIGVAVGTDKGLVVPVVRDCDHKSIADIEKGIADFGRRARDGQLK
IDEMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHKIQERPMVVGGKIEVRPMMYLALSYDHRVIDGKEAVTFLVRVK
ESLEDPARLVLDL
>Mature_412_residues
TDIRVPTLGESVTEATIGRWFKKAGDAVAVDEPLVELETDKVTIEVPAPSAGTLGEIIAKDGETVAVGALLGQINDGAAA
AKPAAAAPAPAPAKPAAAAPAPAPAPAKALPADTPQAPSVRKLSAESGVDATTVPGSGKDGRVTKGDMLAAIERAASAPT
PVNQPAASVQVRAPSPADDAAREERVKMTRLRQTIARRLKDVQNTAAMLTTFNEVDMTHVMALRSQYKDVFEKKHGSKLG
FMGFFTKAVVQALKDIPAVNAEIDGSDLIYKNYYHIGVAVGTDKGLVVPVVRDCDHKSIADIEKGIADFGRRARDGQLKI
DEMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHKIQERPMVVGGKIEVRPMMYLALSYDHRVIDGKEAVTFLVRVKE
SLEDPARLVLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=254, Percent_Identity=53.5433070866142, Blast_Score=288, Evalue=9e-78,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=27.9735682819383, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI203098816, Length=454, Percent_Identity=27.9735682819383, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI110671329, Length=432, Percent_Identity=26.8518518518519, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI31711992, Length=434, Percent_Identity=29.9539170506912, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=34.1317365269461, Blast_Score=102, Evalue=9e-22,
Organism=Escherichia coli, GI1786946, Length=411, Percent_Identity=48.661800486618, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1786305, Length=428, Percent_Identity=34.5794392523364, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI25146366, Length=411, Percent_Identity=43.7956204379562, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17560088, Length=443, Percent_Identity=30.4740406320542, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=430, Percent_Identity=25.8139534883721, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=28.6644951140065, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=416, Percent_Identity=44.4711538461538, Blast_Score=338, Evalue=8e-94,
Organism=Saccharomyces cerevisiae, GI6324258, Length=456, Percent_Identity=26.0964912280702, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24645909, Length=249, Percent_Identity=55.0200803212851, Blast_Score=284, Evalue=7e-77,
Organism=Drosophila melanogaster, GI18859875, Length=420, Percent_Identity=31.1904761904762, Blast_Score=174, Evalue=9e-44,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=30.9012875536481, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=30.9012875536481, Blast_Score=114, Evalue=8e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43583; Mature: 43452

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIRVPTLGESVTEATIGRWFKKAGDAVAVDEPLVELETDKVTIEVPAPSAGTLGEIIA
CCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEECCEEEEEECCCCCCCHHHHHH
KDGETVAVGALLGQINDGAAAAKPAAAAPAPAPAKPAAAAPAPAPAPAKALPADTPQAPS
CCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VRKLSAESGVDATTVPGSGKDGRVTKGDMLAAIERAASAPTPVNQPAASVQVRAPSPADD
HHHHHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHH
AAREERVKMTRLRQTIARRLKDVQNTAAMLTTFNEVDMTHVMALRSQYKDVFEKKHGSKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GFMGFFTKAVVQALKDIPAVNAEIDGSDLIYKNYYHIGVAVGTDKGLVVPVVRDCDHKSI
CHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCHH
ADIEKGIADFGRRARDGQLKIDEMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHKIQ
HHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCEEEHHHHCCCCCCCCCCCCCHHHHC
ERPMVVGGKIEVRPMMYLALSYDHRVIDGKEAVTFLVRVKESLEDPARLVLDL
CCCEEECCEEEECEEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHHHEECC
>Mature Secondary Structure 
TDIRVPTLGESVTEATIGRWFKKAGDAVAVDEPLVELETDKVTIEVPAPSAGTLGEIIA
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEECCEEEEEECCCCCCCHHHHHH
KDGETVAVGALLGQINDGAAAAKPAAAAPAPAPAKPAAAAPAPAPAPAKALPADTPQAPS
CCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VRKLSAESGVDATTVPGSGKDGRVTKGDMLAAIERAASAPTPVNQPAASVQVRAPSPADD
HHHHHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHH
AAREERVKMTRLRQTIARRLKDVQNTAAMLTTFNEVDMTHVMALRSQYKDVFEKKHGSKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GFMGFFTKAVVQALKDIPAVNAEIDGSDLIYKNYYHIGVAVGTDKGLVVPVVRDCDHKSI
CHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCHH
ADIEKGIADFGRRARDGQLKIDEMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHKIQ
HHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCEEEHHHHCCCCCCCCCCCCCHHHHC
ERPMVVGGKIEVRPMMYLALSYDHRVIDGKEAVTFLVRVKESLEDPARLVLDL
CCCEEECCEEEECEEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]