Click here to switch to the map view.

The map label for this gene is leuC [H]

Identifier: 146337520

GI number: 146337520

Start: 379156

End: 380562

Strand: Reverse

Name: leuC [H]

Synonym: BRADO0374

Alternate gene names: 146337520

Gene position: 380562-379156 (Counterclockwise)

Preceding gene: 146337521

Following gene: 146337519

Centisome position: 5.1

GC content: 66.45

Gene sequence:

>1407_bases
ATGTCGAAGCCGACTACCCTGTACGACAAGATCTGGAACGACCATCTGGTACACGAAGCCGAGGACGGCACCTGCCTGCT
CTACATCGACCGCCATCTGGTCCACGAGGTCACCTCGCCCCAGGCGTTCGAGGGCCTGCGCATGACCGGCCGCAAAGTGC
ATGCGCCGGAGAAGACTCTGGCCGTCGTCGATCACAACGTGCCGACCACCGACCGCTCCAAGCCGAACCCGGATCCGGAG
AGCATCGAGCAGATCAAGACGCTGGCCGAGAACGCCAAGGACTTCGGCATCGAGTACTACAACGAGTTCGACCGCCGCCA
GGGCATCGTCCACGTCATCGGCCCGGAGCAGGGTTTTACGCTGCCCGGCACCACGATCGTCTGCGGTGACAGCCACACCT
CGACGCATGGCGCGTTCGGCGCGCTGGCGCACGGCATCGGCACCTCCGAGGTCGAGCATGTGCTGGCGACGCAGACGCTG
ATCCAGAAGAAGGCGAAGAACATGCGCGTCACCGTCGACGGCAAGCTGCCGGACGGCGTGACGGGCAAGGACATCATCCT
CGCCATCATCGGCGAGATCGGCACCGCGGGCGGCACCGGCTACGTGCTGGAATATGCCGGCGAAGCGATCCGCGCGCTGT
CGATGGAAGGCCGCATGACCGTCTGCAACATGTCGATCGAGGGCGGCGCACGCGCCGGCCTGGTCGCGCCGGACGAGAAG
GCCTACGACTTCCTACGCGGCCGCCCCAAGGCGCCGCAGGGCGCGGCCTGGGATGCGGCGATGCGCTACTGGGAGACCTT
GCGTTCCGATGAGGGCGCGCATTTCGACCACGAGCTGCGGCTCGATGCCGCCAAGCTGCCGCCGATCGTGACCTGGGGCA
CCTCGCCCGAGGACGTGATCTCGGTGACCGGCATGGTGCCGGATCCCGACAAGATCGCGGATGAGGCCAAGCGCATCTCC
AAGCATCGCGCGCTGAAATACATGGGCCTGACCGCGGGCACGAAGATCACCGACATCAAGCTCGACCGCGTCTTCATCGG
CTCCTGCACCAATGGCCGCATCGAGGACCTGCGCGCCGCCGCCAAGATCGCCGAGGGCAAGACCGTCTCTAGCCACGTCA
ACGCGATGGTCGTGCCGGGCTCGGGCATCGTCAAGGAGCAGGCCGAGGCCGAGGGTCTGGACAAGATCTTCATCAAGGCC
GGCTTCGAATGGCGCGAGCCGGGCTGCTCCATGTGTCTCGCGATGAACCCGGATAAGCTGGCGCCGGAAGAGCGCTGCGC
CTCGACCTCGAACCGCAACTTCGAGGGCCGTCAGGGCTTCAAGGGCCGTACCCATCTGGTGTCGCCCGCGATGGCCGCGG
CCGCCGCGATCGCCGGTCACTTCGTCGACGTGCGCGACTGGCGCTGA

Upstream 100 bases:

>100_bases
GCTTAGGCGCGCGCTGTAAGCCGCAGCGCCTCCTCCTGCTTGTTCGCGCGTGCCAGTCACGCGTTCAGTTTCAAACCTTC
ATTCCCGGGAGCATTCGCTC

Downstream 100 bases:

>100_bases
GCTGATTCCGCTGGCGCAGCAGAACTGCACAGCGGCCGATCTATAAGGAATGCGGCGGCTTCAGAAGGAGCCGCCGCATT
TTCATTTCGACTCAACATCG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRMTGRKVHAPEKTLAVVDHNVPTTDRSKPNPDPE
SIEQIKTLAENAKDFGIEYYNEFDRRQGIVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
IQKKAKNMRVTVDGKLPDGVTGKDIILAIIGEIGTAGGTGYVLEYAGEAIRALSMEGRMTVCNMSIEGGARAGLVAPDEK
AYDFLRGRPKAPQGAAWDAAMRYWETLRSDEGAHFDHELRLDAAKLPPIVTWGTSPEDVISVTGMVPDPDKIADEAKRIS
KHRALKYMGLTAGTKITDIKLDRVFIGSCTNGRIEDLRAAAKIAEGKTVSSHVNAMVVPGSGIVKEQAEAEGLDKIFIKA
GFEWREPGCSMCLAMNPDKLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDVRDWR

Sequences:

>Translated_468_residues
MSKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRMTGRKVHAPEKTLAVVDHNVPTTDRSKPNPDPE
SIEQIKTLAENAKDFGIEYYNEFDRRQGIVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
IQKKAKNMRVTVDGKLPDGVTGKDIILAIIGEIGTAGGTGYVLEYAGEAIRALSMEGRMTVCNMSIEGGARAGLVAPDEK
AYDFLRGRPKAPQGAAWDAAMRYWETLRSDEGAHFDHELRLDAAKLPPIVTWGTSPEDVISVTGMVPDPDKIADEAKRIS
KHRALKYMGLTAGTKITDIKLDRVFIGSCTNGRIEDLRAAAKIAEGKTVSSHVNAMVVPGSGIVKEQAEAEGLDKIFIKA
GFEWREPGCSMCLAMNPDKLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDVRDWR
>Mature_467_residues
SKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRMTGRKVHAPEKTLAVVDHNVPTTDRSKPNPDPES
IEQIKTLAENAKDFGIEYYNEFDRRQGIVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLI
QKKAKNMRVTVDGKLPDGVTGKDIILAIIGEIGTAGGTGYVLEYAGEAIRALSMEGRMTVCNMSIEGGARAGLVAPDEKA
YDFLRGRPKAPQGAAWDAAMRYWETLRSDEGAHFDHELRLDAAKLPPIVTWGTSPEDVISVTGMVPDPDKIADEAKRISK
HRALKYMGLTAGTKITDIKLDRVFIGSCTNGRIEDLRAAAKIAEGKTVSSHVNAMVVPGSGIVKEQAEAEGLDKIFIKAG
FEWREPGCSMCLAMNPDKLAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDVRDWR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=476, Percent_Identity=25.4201680672269, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI41352693, Length=381, Percent_Identity=24.6719160104987, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=64.14686825054, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=26.0387811634349, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87081781, Length=347, Percent_Identity=25.3602305475504, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=480, Percent_Identity=27.0833333333333, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI32564738, Length=389, Percent_Identity=28.5347043701799, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=309, Percent_Identity=28.8025889967638, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=371, Percent_Identity=27.7628032345013, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=64.5435244161359, Blast_Score=622, Evalue=1e-179,
Organism=Saccharomyces cerevisiae, GI6320440, Length=429, Percent_Identity=25.6410256410256, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6322261, Length=481, Percent_Identity=26.8191268191268, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323335, Length=361, Percent_Identity=29.0858725761773, Blast_Score=120, Evalue=5e-28,
Organism=Drosophila melanogaster, GI281365315, Length=485, Percent_Identity=26.8041237113402, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17864292, Length=485, Percent_Identity=26.8041237113402, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28571643, Length=484, Percent_Identity=27.2727272727273, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI161076999, Length=384, Percent_Identity=27.8645833333333, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI17137564, Length=374, Percent_Identity=25.668449197861, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24645686, Length=379, Percent_Identity=26.3852242744063, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50830; Mature: 50699

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRMTGRKVHAPEKTL
CCCCCHHHHHHCCCCCCEECCCCEEEEEECHHHHHHCCCCHHHHHHHHCCCEECCCCCEE
AVVDHNVPTTDRSKPNPDPESIEQIKTLAENAKDFGIEYYNEFDRRQGIVHVIGPEQGFT
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEECCCCCCC
LPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMRVTVDGKLPDGV
CCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
TGKDIILAIIGEIGTAGGTGYVLEYAGEAIRALSMEGRMTVCNMSIEGGARAGLVAPDEK
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHH
AYDFLRGRPKAPQGAAWDAAMRYWETLRSDEGAHFDHELRLDAAKLPPIVTWGTSPEDVI
HHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECHHHCCCEEECCCCHHHHE
SVTGMVPDPDKIADEAKRISKHRALKYMGLTAGTKITDIKLDRVFIGSCTNGRIEDLRAA
EEECCCCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEEEEEEECCCCCHHHHHHHH
AKIAEGKTVSSHVNAMVVPGSGIVKEQAEAEGLDKIFIKAGFEWREPGCSMCLAMNPDKL
HHHHCCCEECCCCCEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCCC
APEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDVRDWR
CCHHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHEEEECCCC
>Mature Secondary Structure 
SKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRMTGRKVHAPEKTL
CCCCHHHHHHCCCCCCEECCCCEEEEEECHHHHHHCCCCHHHHHHHHCCCEECCCCCEE
AVVDHNVPTTDRSKPNPDPESIEQIKTLAENAKDFGIEYYNEFDRRQGIVHVIGPEQGFT
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEECCCCCCC
LPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMRVTVDGKLPDGV
CCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
TGKDIILAIIGEIGTAGGTGYVLEYAGEAIRALSMEGRMTVCNMSIEGGARAGLVAPDEK
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHH
AYDFLRGRPKAPQGAAWDAAMRYWETLRSDEGAHFDHELRLDAAKLPPIVTWGTSPEDVI
HHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECHHHCCCEEECCCCHHHHE
SVTGMVPDPDKIADEAKRISKHRALKYMGLTAGTKITDIKLDRVFIGSCTNGRIEDLRAA
EEECCCCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEEEEEEECCCCCHHHHHHHH
AKIAEGKTVSSHVNAMVVPGSGIVKEQAEAEGLDKIFIKAGFEWREPGCSMCLAMNPDKL
HHHHCCCEECCCCCEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCCC
APEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDVRDWR
CCHHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]