Click here to switch to the map view.

The map label for this gene is argG

Identifier: 146337407

GI number: 146337407

Start: 260750

End: 262096

Strand: Reverse

Name: argG

Synonym: BRADO0243

Alternate gene names: 146337407

Gene position: 262096-260750 (Counterclockwise)

Preceding gene: 146337408

Following gene: 146337396

Centisome position: 3.51

GC content: 64.59

Gene sequence:

>1347_bases
ATGACCACGATCCTCAAAGGCCTGCCTACGGGCGAGAAAGTCGGCATCGCCTTCTCCGGCGGCCTCGACACCAGCGCGGC
GCTGCTCTGGATGAAGCAGAAGGGCGCGCGCTGCTTCGCCTACACCGCCAATCTCGGGCAGCCCGACGAGTCCGACTATG
ACGAGATCCCGCGCAAGGCGATGAGCTTCGGCGCCGAGAAGGCGCGCCTGGTCGATTGCCGCACCCAGCTGGTGCATGAG
GGCATCGCCGCGATCCAGTCCGGCGCCTTCCACATCTCGACCGGCGGCGCGACCTATTTCAACACCACGCCGCTCGGCCG
CGCCGTCACCGGCACCATGCTGGTGGCCGCGATGAAGGAGGACGGCGTCAACATCTGGGGCGACGGCTCGACCTACAAGG
GCAACGACATCGAGCGCTTCTACCGCTACGGCCTGCTGACCAACCCGAACCTCAAGATCTACAAGCCCTGGCTCGACCAG
CAGTTCATCGACGAGCTCGGCGGCCGCGCCGAGATGTCGGCATTCCTCACCGCCCACGGCTTCAATTACAAGATGAGCGC
CGAAAAGGCGTATTCGACCGACAGCAATCTGCTCGGCGCCACTCACGAGGCGAAGGATCTCGAAAGCCTGTCGAGCGGCA
TCCGCATCGTCAATCCGATCATGGGCGTGCCGTTCTGGCGTGAGGACTGCGCGGTGAGGCCCGAGACCGTCGTGGTGCGC
TTCGAGGAAGGCCAGCCGGTCGCGCTGAACGGCCAGACATTCACAGATCCCGTCGCGCTGTTCCTGGAGGCCAATGCGAT
CGGCGGCCGCCATGGCCTGGGAATGTGCGACCAGATCGAGAACCGCATCATCGAGGCCAAGAGCCGCGGGATCTACGAGG
CGCCCGGCATGGCGCTGCTGCACATTGCCTATGAGCGGCTCGTCACCGGCATCCACAACGAGGATACGATCGAGCAGTAC
CGCATGAGCGGCATGAAGCTTGGCCGTCTGCTGTATCAAGGCCGTTGGTTCGACTCGCAGGCCTTGATGCTGCGCGAGAC
CGCGCAGCGCTGGGTCGCCAGCGCCATCACCGGCGAGGTGACTTTGGAGCTGCGCCGCGGCAACGACTACTCGCTGCTCA
ACACCGAGAGCCCCAACCTGACCTATCAGCCGGAGCGGCTGAGCATGGAAAAGGTCGAGGACGCCGCCTTCACGCCCGCC
GACCGTATCGGCCAGCTGACCATGCGCAACCTCGACATCACCGACACCCGCACCAAGCTGAAGCTCTATTCGGACACGGG
CCTGCTGTCTGGCGCGGAGGGCGCTCAAATTTTCCAGCTCGGCCATGACAAGGGCGACAAGAGCTGA

Upstream 100 bases:

>100_bases
AACAGGCCCCCTGCGGGATTAATCGCGTGCCGAGACGGGCGAAATCTGCTATCCGCGCCCGAATTTCTGAACGGCACAGC
CATCAACACCGGTACTTCCC

Downstream 100 bases:

>100_bases
GCGGAGCGTTTCGCAGCGAGACGCGCACCACGTTCTCCGGCGTCGCCCCGGCGAAAGCCGGGACCCATAACCACCACTTG
CAATGGTGAAGGCTGGCTGT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MTTILKGLPTGEKVGIAFSGGLDTSAALLWMKQKGARCFAYTANLGQPDESDYDEIPRKAMSFGAEKARLVDCRTQLVHE
GIAAIQSGAFHISTGGATYFNTTPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNPNLKIYKPWLDQ
QFIDELGGRAEMSAFLTAHGFNYKMSAEKAYSTDSNLLGATHEAKDLESLSSGIRIVNPIMGVPFWREDCAVRPETVVVR
FEEGQPVALNGQTFTDPVALFLEANAIGGRHGLGMCDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQY
RMSGMKLGRLLYQGRWFDSQALMLRETAQRWVASAITGEVTLELRRGNDYSLLNTESPNLTYQPERLSMEKVEDAAFTPA
DRIGQLTMRNLDITDTRTKLKLYSDTGLLSGAEGAQIFQLGHDKGDKS

Sequences:

>Translated_448_residues
MTTILKGLPTGEKVGIAFSGGLDTSAALLWMKQKGARCFAYTANLGQPDESDYDEIPRKAMSFGAEKARLVDCRTQLVHE
GIAAIQSGAFHISTGGATYFNTTPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNPNLKIYKPWLDQ
QFIDELGGRAEMSAFLTAHGFNYKMSAEKAYSTDSNLLGATHEAKDLESLSSGIRIVNPIMGVPFWREDCAVRPETVVVR
FEEGQPVALNGQTFTDPVALFLEANAIGGRHGLGMCDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQY
RMSGMKLGRLLYQGRWFDSQALMLRETAQRWVASAITGEVTLELRRGNDYSLLNTESPNLTYQPERLSMEKVEDAAFTPA
DRIGQLTMRNLDITDTRTKLKLYSDTGLLSGAEGAQIFQLGHDKGDKS
>Mature_447_residues
TTILKGLPTGEKVGIAFSGGLDTSAALLWMKQKGARCFAYTANLGQPDESDYDEIPRKAMSFGAEKARLVDCRTQLVHEG
IAAIQSGAFHISTGGATYFNTTPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNPNLKIYKPWLDQQ
FIDELGGRAEMSAFLTAHGFNYKMSAEKAYSTDSNLLGATHEAKDLESLSSGIRIVNPIMGVPFWREDCAVRPETVVVRF
EEGQPVALNGQTFTDPVALFLEANAIGGRHGLGMCDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYR
MSGMKLGRLLYQGRWFDSQALMLRETAQRWVASAITGEVTLELRRGNDYSLLNTESPNLTYQPERLSMEKVEDAAFTPAD
RIGQLTMRNLDITDTRTKLKLYSDTGLLSGAEGAQIFQLGHDKGDKS

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=414, Percent_Identity=30.1932367149758, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI16950633, Length=414, Percent_Identity=30.1932367149758, Blast_Score=162, Evalue=8e-40,
Organism=Escherichia coli, GI1789563, Length=440, Percent_Identity=78.4090909090909, Blast_Score=715, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324514, Length=421, Percent_Identity=29.4536817102138, Blast_Score=159, Evalue=8e-40,
Organism=Drosophila melanogaster, GI21358151, Length=417, Percent_Identity=27.5779376498801, Blast_Score=166, Evalue=4e-41,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_BRASO (A4YJX9)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001202455.1
- STRING:   A4YJX9
- GeneID:   5116656
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO0243
- eggNOG:   COG0137
- HOGENOM:   HBG289560
- OMA:   GGRKEMS
- ProtClustDB:   PRK05370
- BioCyc:   BSP376:BRADO0243-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00581
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 49428; Mature: 49296

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 43-43 BINDING 99-99 BINDING 129-129 BINDING 131-131 BINDING 131-131 BINDING 135-135 BINDING 135-135 BINDING 136-136 BINDING 136-136 BINDING 139-139 BINDING 192-192 BINDING 194-194 BINDING 201-201 BINDING 203-203 BINDING 280-280

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTILKGLPTGEKVGIAFSGGLDTSAALLWMKQKGARCFAYTANLGQPDESDYDEIPRKA
CCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHH
MSFGAEKARLVDCRTQLVHEGIAAIQSGAFHISTGGATYFNTTPLGRAVTGTMLVAAMKE
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCHHHHHHHHEEEHHC
DGVNIWGDGSTYKGNDIERFYRYGLLTNPNLKIYKPWLDQQFIDELGGRAEMSAFLTAHG
CCCEEECCCCCCCCCHHHHHHHHCCEECCCCEEECCCCCHHHHHHHCCHHHHHHEEEECC
FNYKMSAEKAYSTDSNLLGATHEAKDLESLSSGIRIVNPIMGVPFWREDCAVRPETVVVR
CCEEECCCHHCCCCCCCCCCCCCHHHHHHHHCCCEEEHHHHCCCCCCCCCCCCCCEEEEE
FEEGQPVALNGQTFTDPVALFLEANAIGGRHGLGMCDQIENRIIEAKSRGIYEAPGMALL
ECCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHH
HIAYERLVTGIHNEDTIEQYRMSGMKLGRLLYQGRWFDSQALMLRETAQRWVASAITGEV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEE
TLELRRGNDYSLLNTESPNLTYQPERLSMEKVEDAAFTPADRIGQLTMRNLDITDTRTKL
EEEEECCCCEEEEECCCCCCEECHHHCCHHHHHHHCCCCHHHHCCEEECCCCCCCCCEEE
KLYSDTGLLSGAEGAQIFQLGHDKGDKS
EEEECCCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
TTILKGLPTGEKVGIAFSGGLDTSAALLWMKQKGARCFAYTANLGQPDESDYDEIPRKA
CCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHH
MSFGAEKARLVDCRTQLVHEGIAAIQSGAFHISTGGATYFNTTPLGRAVTGTMLVAAMKE
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCHHHHHHHHEEEHHC
DGVNIWGDGSTYKGNDIERFYRYGLLTNPNLKIYKPWLDQQFIDELGGRAEMSAFLTAHG
CCCEEECCCCCCCCCHHHHHHHHCCEECCCCEEECCCCCHHHHHHHCCHHHHHHEEEECC
FNYKMSAEKAYSTDSNLLGATHEAKDLESLSSGIRIVNPIMGVPFWREDCAVRPETVVVR
CCEEECCCHHCCCCCCCCCCCCCHHHHHHHHCCCEEEHHHHCCCCCCCCCCCCCCEEEEE
FEEGQPVALNGQTFTDPVALFLEANAIGGRHGLGMCDQIENRIIEAKSRGIYEAPGMALL
ECCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHH
HIAYERLVTGIHNEDTIEQYRMSGMKLGRLLYQGRWFDSQALMLRETAQRWVASAITGEV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEE
TLELRRGNDYSLLNTESPNLTYQPERLSMEKVEDAAFTPADRIGQLTMRNLDITDTRTKL
EEEEECCCCEEEEECCCCCCEECHHHCCHHHHHHHCCCCHHHHCCEEECCCCCCCCCEEE
KLYSDTGLLSGAEGAQIFQLGHDKGDKS
EEEECCCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA