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The map label for this gene is mhpD2 [H]

Identifier: 146337352

GI number: 146337352

Start: 196040

End: 196825

Strand: Direct

Name: mhpD2 [H]

Synonym: BRADO0185

Alternate gene names: 146337352

Gene position: 196040-196825 (Clockwise)

Preceding gene: 146337351

Following gene: 146337354

Centisome position: 2.63

GC content: 68.19

Gene sequence:

>786_bases
ATGCGAGCGACCGATCAGCCTGCCGGCGCCTCGGCCATCCTACGCTGGCATTGGCTGCAGGGAAGCAAGCTGAGCGAGTT
GCCGCCGAAGCTGCGCCCGCGCGACAGGGCGGCGGGCTACGCGATCCAGGCCGAGCTCGAGAAGCACTCGGACAAGCCGT
TGTTCGGCTGGAAGATTGCCGCGACCAGCGAAGCCGGGCAGCGCCATATCAACGTTGCCGGCCCGATGGCCGGGCGCATC
CTCGCCGAGACGGTCCTGCCGGTGGACGGCACGGCCTCGATGGCCGGCAACCTGATGCGCGTCGCCGAGCCGGAATTCGC
CTTTCGCTTCGGCGTCGACCTGCCGCCGCGGCCGACACCCTATGACGTGGCCGAGGTGCTCGCGGCCGTCGACACGCTGC
ACCCGGCGATCGAGATCCCTGATTCGCGGTTCGCCGAGTTCGTCGGCGCCGGCGAAGCGCAACTGATCGCGGACAATGCC
TGTGCGCATCTGTTCGTGCTGGGCGAGCCGACATCAGCCGACTGGCGCAGCCGTGATCTCGTCGAAGAGCGGCCCCGCAT
CTCGCTCAAGGGCGAGACGTTCATAGGCCATGGCCGCAATGTGCTCGGCGATCCCCGGATCGCGCTGGCCTGGCTGGTCA
ACGAGCTGCGCGAGCTTGGCATCACCGTGCGGGCCGGGCAGGTGATAACCACCGGAACCTGCCATCCGCCGCTGCCGATC
ACCGGCGGCGATCACATGGAAGCTGATTTTGGCGACCTGGGGCGGGTGTCGGTCAGGTTCGACTGA

Upstream 100 bases:

>100_bases
TGCGTGGCGATGACCCGGCCGGCGATGCTGCGGCTGTTGCAAAGGATCGGTCCGCGCACCAGGATCTTCATCTCATAAAA
GAGGAGACGGGGAGGACATC

Downstream 100 bases:

>100_bases
CAGATGGCGGTCATCGCGAGCGCGGCTCGGGCGGCACCTCGCCTAGCGGTGGCCGAAGATCGCCGAGCCCACCCGCACAT
GGGTGGCGCCGAACTGGATC

Product: putative hydratase/decarboxylase

Products: NA

Alternate protein names: 2-hydroxypentadienoic acid hydratase 2 [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRATDQPAGASAILRWHWLQGSKLSELPPKLRPRDRAAGYAIQAELEKHSDKPLFGWKIAATSEAGQRHINVAGPMAGRI
LAETVLPVDGTASMAGNLMRVAEPEFAFRFGVDLPPRPTPYDVAEVLAAVDTLHPAIEIPDSRFAEFVGAGEAQLIADNA
CAHLFVLGEPTSADWRSRDLVEERPRISLKGETFIGHGRNVLGDPRIALAWLVNELRELGITVRAGQVITTGTCHPPLPI
TGGDHMEADFGDLGRVSVRFD

Sequences:

>Translated_261_residues
MRATDQPAGASAILRWHWLQGSKLSELPPKLRPRDRAAGYAIQAELEKHSDKPLFGWKIAATSEAGQRHINVAGPMAGRI
LAETVLPVDGTASMAGNLMRVAEPEFAFRFGVDLPPRPTPYDVAEVLAAVDTLHPAIEIPDSRFAEFVGAGEAQLIADNA
CAHLFVLGEPTSADWRSRDLVEERPRISLKGETFIGHGRNVLGDPRIALAWLVNELRELGITVRAGQVITTGTCHPPLPI
TGGDHMEADFGDLGRVSVRFD
>Mature_261_residues
MRATDQPAGASAILRWHWLQGSKLSELPPKLRPRDRAAGYAIQAELEKHSDKPLFGWKIAATSEAGQRHINVAGPMAGRI
LAETVLPVDGTASMAGNLMRVAEPEFAFRFGVDLPPRPTPYDVAEVLAAVDTLHPAIEIPDSRFAEFVGAGEAQLIADNA
CAHLFVLGEPTSADWRSRDLVEERPRISLKGETFIGHGRNVLGDPRIALAWLVNELRELGITVRAGQVITTGTCHPPLPI
TGGDHMEADFGDLGRVSVRFD

Specific function: Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family. MhpD subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=257, Percent_Identity=26.4591439688716, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =4.2.1.80 [H]

Molecular weight: Translated: 28289; Mature: 28289

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRATDQPAGASAILRWHWLQGSKLSELPPKLRPRDRAAGYAIQAELEKHSDKPLFGWKIA
CCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCHHCCEEEEEEHHHCCCCCCEEEEEE
ATSEAGQRHINVAGPMAGRILAETVLPVDGTASMAGNLMRVAEPEFAFRFGVDLPPRPTP
ECCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHCCEEECCCCCEEEEECCCCCCCCCC
YDVAEVLAAVDTLHPAIEIPDSRFAEFVGAGEAQLIADNACAHLFVLGEPTSADWRSRDL
HHHHHHHHHHHHHCCCEECCHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCHHHH
VEERPRISLKGETFIGHGRNVLGDPRIALAWLVNELRELGITVRAGQVITTGTCHPPLPI
HHHCCCEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHCCEEEECCEEEEECCCCCCCCC
TGGDHMEADFGDLGRVSVRFD
CCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MRATDQPAGASAILRWHWLQGSKLSELPPKLRPRDRAAGYAIQAELEKHSDKPLFGWKIA
CCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCHHCCEEEEEEHHHCCCCCCEEEEEE
ATSEAGQRHINVAGPMAGRILAETVLPVDGTASMAGNLMRVAEPEFAFRFGVDLPPRPTP
ECCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHCCEEECCCCCEEEEECCCCCCCCCC
YDVAEVLAAVDTLHPAIEIPDSRFAEFVGAGEAQLIADNACAHLFVLGEPTSADWRSRDL
HHHHHHHHHHHHHCCCEECCHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCHHHH
VEERPRISLKGETFIGHGRNVLGDPRIALAWLVNELRELGITVRAGQVITTGTCHPPLPI
HHHCCCEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHCCEEEECCEEEEECCCCCCCCC
TGGDHMEADFGDLGRVSVRFD
CCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA