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The map label for this gene is pyrF [H]

Identifier: 146337329

GI number: 146337329

Start: 172703

End: 173434

Strand: Reverse

Name: pyrF [H]

Synonym: BRADO0160

Alternate gene names: 146337329

Gene position: 173434-172703 (Counterclockwise)

Preceding gene: 146337330

Following gene: 146337328

Centisome position: 2.33

GC content: 70.08

Gene sequence:

>732_bases
ATGCCGACCGAGATTGCTCCGTGCGACCGCCTGATCGTGGCCCTCGACGTCCCCAGCGTGGCGGACGCCGAGGTGATGAT
CGCAACCCTCGGCGATGCCGTGACGTTCTACAAGATCGGCATGGAGTTGACCTATGCCGGCGGCCTCGGCCTCGCCGAGC
GGCTCGCCGCGGACGGCAAGCAAGTGTTCATGGACCTCAAGCTGCACGACATCCCGAACACCGTCGAGCGCGCCACGCGG
CAGATCGCAAAACTCGGCGTCCGCTTCCTCACCGTGCACGGCTTCTCGCAAAGCATGAAGGCCGCGCTCGCCGGCGCCGC
CGGCTCCCCCCTCGAGCTGCTCGCCGTCACCGTGATGACCTCCTACGACGATGCCGACCTCGCCACCGCCGGCTATGCGA
TGACCGTCAAGGAGCTCGTCGCCCACCGCGCTGTGCAGGCGAGGGACATCGGCATCCACGGCCTGATCCTGTCGCCGGAG
GAGACCCAGCTGGTCCGCCCGCTGGTTGGCCCCGATATGCAGCTCGTCACCCCCGGGATCCGCCCAGCGGGCTCCGACGT
TGGCGACCAGAAGCGCATCATGACCCCGGCGCTGGCGATCGCCGGCGGCGCCGACCGCCTCGTTGTCGGCCGCCCCGTCA
CCGGCGCCGCCGATCCCGCCGCCGCGGCGGAGGCCATCGTCGCCGACATCGCCACCGCCGTCGCGCTGGTCGGCAAGACC
AATCGCTCGTAA

Upstream 100 bases:

>100_bases
CAACGACATGGACCGGCTCAGGCTGCCCGCCGACATCGCCGCGCTCGACGCGCTGGTCAAGCAGCTGATCCATTCCGCCC
AACACACCAATTGAGGTGAC

Downstream 100 bases:

>100_bases
GCAGAAGCGCCTCATACCAAGGAGACCCCAGGGATGCCCAAGGCCTATTGGATCGCCCGCATCGACGTGCACAACATGGA
CGGCTACAAGGAATACGTCG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MPTEIAPCDRLIVALDVPSVADAEVMIATLGDAVTFYKIGMELTYAGGLGLAERLAADGKQVFMDLKLHDIPNTVERATR
QIAKLGVRFLTVHGFSQSMKAALAGAAGSPLELLAVTVMTSYDDADLATAGYAMTVKELVAHRAVQARDIGIHGLILSPE
ETQLVRPLVGPDMQLVTPGIRPAGSDVGDQKRIMTPALAIAGGADRLVVGRPVTGAADPAAAAEAIVADIATAVALVGKT
NRS

Sequences:

>Translated_243_residues
MPTEIAPCDRLIVALDVPSVADAEVMIATLGDAVTFYKIGMELTYAGGLGLAERLAADGKQVFMDLKLHDIPNTVERATR
QIAKLGVRFLTVHGFSQSMKAALAGAAGSPLELLAVTVMTSYDDADLATAGYAMTVKELVAHRAVQARDIGIHGLILSPE
ETQLVRPLVGPDMQLVTPGIRPAGSDVGDQKRIMTPALAIAGGADRLVVGRPVTGAADPAAAAEAIVADIATAVALVGKT
NRS
>Mature_242_residues
PTEIAPCDRLIVALDVPSVADAEVMIATLGDAVTFYKIGMELTYAGGLGLAERLAADGKQVFMDLKLHDIPNTVERATRQ
IAKLGVRFLTVHGFSQSMKAALAGAAGSPLELLAVTVMTSYDDADLATAGYAMTVKELVAHRAVQARDIGIHGLILSPEE
TQLVRPLVGPDMQLVTPGIRPAGSDVGDQKRIMTPALAIAGGADRLVVGRPVTGAADPAAAAEAIVADIATAVALVGKTN
RS

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=226, Percent_Identity=37.1681415929204, Blast_Score=134, Evalue=5e-33,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25160; Mature: 25029

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTEIAPCDRLIVALDVPSVADAEVMIATLGDAVTFYKIGMELTYAGGLGLAERLAADGK
CCCCCCCCCEEEEEECCCCCCCCCEEEEHHCCHHEEEEECEEEEECCCCCHHHHHHCCCC
QVFMDLKLHDIPNTVERATRQIAKLGVRFLTVHGFSQSMKAALAGAAGSPLELLAVTVMT
EEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHH
SYDDADLATAGYAMTVKELVAHRAVQARDIGIHGLILSPEETQLVRPLVGPDMQLVTPGI
CCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCEEECCCC
RPAGSDVGDQKRIMTPALAIAGGADRLVVGRPVTGAADPAAAAEAIVADIATAVALVGKT
CCCCCCCCCCHHHHHHHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
NRS
CCC
>Mature Secondary Structure 
PTEIAPCDRLIVALDVPSVADAEVMIATLGDAVTFYKIGMELTYAGGLGLAERLAADGK
CCCCCCCCEEEEEECCCCCCCCCEEEEHHCCHHEEEEECEEEEECCCCCHHHHHHCCCC
QVFMDLKLHDIPNTVERATRQIAKLGVRFLTVHGFSQSMKAALAGAAGSPLELLAVTVMT
EEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHH
SYDDADLATAGYAMTVKELVAHRAVQARDIGIHGLILSPEETQLVRPLVGPDMQLVTPGI
CCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCEEECCCC
RPAGSDVGDQKRIMTPALAIAGGADRLVVGRPVTGAADPAAAAEAIVADIATAVALVGKT
CCCCCCCCCCHHHHHHHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
NRS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA