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The map label for this gene is emrB [H]

Identifier: 146337318

GI number: 146337318

Start: 163038

End: 164657

Strand: Reverse

Name: emrB [H]

Synonym: BRADO0149

Alternate gene names: 146337318

Gene position: 164657-163038 (Counterclockwise)

Preceding gene: 146337319

Following gene: 146337315

Centisome position: 2.21

GC content: 68.02

Gene sequence:

>1620_bases
ATGAGCGCAGCCGCCGAGCCGGAGGATAGACCGAGCCTCGCCACCTGGCTCGGCTTCGTCCTGATGTGCGTCGGCATGTT
CATGGCCATCCTGGACATCCAGGTGGTAGCGACGTCGCTGCCGACCATCCAGGAGGCGCTCGGCATCACGCCGGACGCGA
TGAGCTGGATCCAGACGGCGTATCTGATCGCCGAGATCATCGCGATTCCGCTGACCGGCCTGCTGACGCGCGTGTTCACC
TTGCGCTGGCTGTTCACAGGCGCCGTCAGCGTGTTCACGGCAGCTTCGCTCGGCTGCGCGCTGAGCGGCAGCTTTCACAT
GCTGCTGGCGTTCCGCGTACTGCAGGGATTTTTCGGGGGCCTCCTGATCCCCGTGGTGTTCTCCGCGGTGTTCCTGCTGT
TTCCGTCGCGTCTGCATGCGGTGGCCACCACGATTGCTGGCGTGGTCGCGGTGCTCGCGCCGACGATCGGGCCCGTGGTC
GGCGGCTTCATCACCAACACCTGGTCATGGCCGTGGCTGTTCCTGATCAACATCGTCCCTGGCCTCATCGCGGCGGCCGC
CACGCCGGCGCTGCTGCCGAAGCAGGGCATGGATCTGGAGGAGCTCGACAAGCTCGATCTGCTGGCGCTGCTGCTGCTCG
CGGTGTCGCTGGCAGGCCTCGAGCTCGGGCTGAAGGCGGCACCGAAGAGCGGCTGGCTGTCGTCGAACTGCCTCGCGCTT
CTGGTGCTCAGCGCGTCATGCCTCACGCTGCTGATCCAGCGCCTGCTCGCCGCGGCTGATCCGATCCTGCGCATCGGCAG
CTTCCAGCGCCGCGCCTTCACGCTCGGCTGCGTGTCGAGTTTCTGCCTGGGCATCGGCCTGTTCGGCTCGGTCTATCTGA
TGCCGGTGTTCCTCGCCTTCGTGCGCGGCCATGACGCGTTCGAGATCGGCAAGATCATGCTGGTGACGGGAGTCGCGCAG
CTGGTCGCCGCGCCGCTGGTGACGGCGCTCGACGGCAAGGTCGACGCGCGGCTGCTGACGGCGTTCGGCTTCGTGCTGTT
CAGCGCCGGCCTGGCGGCGAGCGCGTTCCAGCCGCCGAGCGCCGACTATCAGGAGATGTTCTGGCCGCAGGTCGTGCGCG
GCGTCGGCATCATGTTCTGCCTCTTGCCGCCGACGCGGATCGCGCTTGGCGACCTGCCGCCCGCCGAGGTGGCGGATGCC
AGCGGTTTGTTCAACCTGATGCGCAATCTCGGCGGCGCCATCGGCATCGCGCTGATCGACACCATCATCTACGGCCGCGT
GACGTTGCACGCGCAGGCGTTCCGCGACCGGCTGATGGCCGGCGACACCGCCGCCGCCAAGGCGATCGGTCTCGCGCCGG
AACTGCTGCGCAACCGTCCCCGCAACATCTCCGAGGAGCAGGCGATCGCCTATGTCCGCCCGCTGGTGGAGAAGGCCTCG
CTGGCGCTGTGCGTCAACGAGGCGTGGTCGCTGCTCGCCGGCATCGCGCTGCTCGGCTTTCTGCTGATCCCGTTCGCGCG
CAACAAACCTGTCAGCGCGACGCCGCAGCGGATGGCGCTAGAGGCTGCGCAGCCGTCGCTCCGGCGACGGCTCGATCAGC
TCGGGTCGCGGTCCGGATAG

Upstream 100 bases:

>100_bases
TCTTCTCCGGGCTGAACGCCGCCGTGCTGTTCATCCGCATCCGCGCCGAATCCGAGGCGCTGTCCGGCGTCGCTGGCGAT
GGCATCGGCGGCCAGACGGC

Downstream 100 bases:

>100_bases
CCGCTTGTGCAGATGGACCATGAAGGCCATGCCGAACAGCGGCGTCGCCAGGTTCACGATAGGGATAGTCACGAAGGCGG
CGATGATCAGTCCCGCGGTG

Product: multidrug ABC transporter membrane protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MSAAAEPEDRPSLATWLGFVLMCVGMFMAILDIQVVATSLPTIQEALGITPDAMSWIQTAYLIAEIIAIPLTGLLTRVFT
LRWLFTGAVSVFTAASLGCALSGSFHMLLAFRVLQGFFGGLLIPVVFSAVFLLFPSRLHAVATTIAGVVAVLAPTIGPVV
GGFITNTWSWPWLFLINIVPGLIAAAATPALLPKQGMDLEELDKLDLLALLLLAVSLAGLELGLKAAPKSGWLSSNCLAL
LVLSASCLTLLIQRLLAAADPILRIGSFQRRAFTLGCVSSFCLGIGLFGSVYLMPVFLAFVRGHDAFEIGKIMLVTGVAQ
LVAAPLVTALDGKVDARLLTAFGFVLFSAGLAASAFQPPSADYQEMFWPQVVRGVGIMFCLLPPTRIALGDLPPAEVADA
SGLFNLMRNLGGAIGIALIDTIIYGRVTLHAQAFRDRLMAGDTAAAKAIGLAPELLRNRPRNISEEQAIAYVRPLVEKAS
LALCVNEAWSLLAGIALLGFLLIPFARNKPVSATPQRMALEAAQPSLRRRLDQLGSRSG

Sequences:

>Translated_539_residues
MSAAAEPEDRPSLATWLGFVLMCVGMFMAILDIQVVATSLPTIQEALGITPDAMSWIQTAYLIAEIIAIPLTGLLTRVFT
LRWLFTGAVSVFTAASLGCALSGSFHMLLAFRVLQGFFGGLLIPVVFSAVFLLFPSRLHAVATTIAGVVAVLAPTIGPVV
GGFITNTWSWPWLFLINIVPGLIAAAATPALLPKQGMDLEELDKLDLLALLLLAVSLAGLELGLKAAPKSGWLSSNCLAL
LVLSASCLTLLIQRLLAAADPILRIGSFQRRAFTLGCVSSFCLGIGLFGSVYLMPVFLAFVRGHDAFEIGKIMLVTGVAQ
LVAAPLVTALDGKVDARLLTAFGFVLFSAGLAASAFQPPSADYQEMFWPQVVRGVGIMFCLLPPTRIALGDLPPAEVADA
SGLFNLMRNLGGAIGIALIDTIIYGRVTLHAQAFRDRLMAGDTAAAKAIGLAPELLRNRPRNISEEQAIAYVRPLVEKAS
LALCVNEAWSLLAGIALLGFLLIPFARNKPVSATPQRMALEAAQPSLRRRLDQLGSRSG
>Mature_538_residues
SAAAEPEDRPSLATWLGFVLMCVGMFMAILDIQVVATSLPTIQEALGITPDAMSWIQTAYLIAEIIAIPLTGLLTRVFTL
RWLFTGAVSVFTAASLGCALSGSFHMLLAFRVLQGFFGGLLIPVVFSAVFLLFPSRLHAVATTIAGVVAVLAPTIGPVVG
GFITNTWSWPWLFLINIVPGLIAAAATPALLPKQGMDLEELDKLDLLALLLLAVSLAGLELGLKAAPKSGWLSSNCLALL
VLSASCLTLLIQRLLAAADPILRIGSFQRRAFTLGCVSSFCLGIGLFGSVYLMPVFLAFVRGHDAFEIGKIMLVTGVAQL
VAAPLVTALDGKVDARLLTAFGFVLFSAGLAASAFQPPSADYQEMFWPQVVRGVGIMFCLLPPTRIALGDLPPAEVADAS
GLFNLMRNLGGAIGIALIDTIIYGRVTLHAQAFRDRLMAGDTAAAKAIGLAPELLRNRPRNISEEQAIAYVRPLVEKASL
ALCVNEAWSLLAGIALLGFLLIPFARNKPVSATPQRMALEAAQPSLRRRLDQLGSRSG

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=483, Percent_Identity=28.3643892339545, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1788710, Length=495, Percent_Identity=29.4949494949495, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87081983, Length=402, Percent_Identity=29.8507462686567, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1790195, Length=392, Percent_Identity=27.0408163265306, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1788392, Length=424, Percent_Identity=29.2452830188679, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57174; Mature: 57042

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SAAAEPEDRPSLATWLGFVLMCVGMFMAILDIQVVATSLPTIQEALGITPDAMSWIQTA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]