Click here to switch to the map view.

The map label for this gene is dagK [H]

Identifier: 146300800

GI number: 146300800

Start: 3617483

End: 3618307

Strand: Direct

Name: dagK [H]

Synonym: Fjoh_3052

Alternate gene names: 146300800

Gene position: 3617483-3618307 (Clockwise)

Preceding gene: 146300799

Following gene: 146300806

Centisome position: 59.33

GC content: 51.39

Gene sequence:

>825_bases
ATGCCCGATTTTTACATCAGGCAGTTTTTTCCCTCTGACCGGTATAAGATCAGGACAGACTACACCATGAGCAGAAAGCA
CGCGCTTGTCCTCACGCAGAAGGCCCTCGAGCATAATCCCGACATTATCGTCGCCTGCGGAGGCGACGGAACCGTAAATG
AGGTGGCTTCCTGCCTGATCGGCACCGATGTGAAGCTGGGAATCATCCCGCTGGGGTCGGGAAACGGCCTGGCCTCGCAT
CTGAACATACCGCATGAGATCGGAAAATCCCTTGAAGTGATCAGGGAAGGAAGGAAAATAAGAATAGATGCGGGAAGGAT
CAACCAGCATTATTTTTTCAGCAACACCGGCATCGGCATCGACGCCATGATCATCAGAAAGTACGAGCATTCCGGAAAAA
GGATGCTTTTTGCCTATGTAAAGGCCGCAGTTGCGGCAGCCTTTGAGTACAGGGCACAGCCGGCCATCGTTTCTTTTGAT
GACAGGGTGATTCCCATCAGGCCTTTTATCGTTTTTGTTTCCAATTCCAATGAAATGGGCTATAATATGACCCTTACCCC
CGATGCGATGCTGACCGACGGAAAACTTGACCTGGTGCTCATTCCCGAGCTTTCCTTTTTCGAAAAGATAGCCCTTGGAT
ACCGCATCCTGACCCGTTCGGTGAGCAGATTCAAAAAGGCACAGCACCATCTGGTGCAGAGCCTGCAGATCGAAATGCCA
ATGAAGATCTTCACCGACGCGCAGATCGACGGGGAGCATTATAAATTAAGGACCAACATCTGCCGGATTTCGGCAGAGCC
TGCCGCACTCAGCGTGCTGGTCTGA

Upstream 100 bases:

>100_bases
CCAGACCGCCGATTACCTTTTTACAAACAAACTTTTAAAATAAGCGATGACCTATATACATTTCATAATAAATCCGGTCT
CCGGAGCAGGACGGCACAGC

Downstream 100 bases:

>100_bases
AAGCTTAATTAGGCTTTTCCGGAACTGCCTTCCCTTCGGGGAAGGCTCTCCGGAAATAAAGGCTTCCGCCCTTGATCCTA
TCAGCTTCATTTGGCAAGCC

Product: diacylglycerol kinase catalytic subunit

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MPDFYIRQFFPSDRYKIRTDYTMSRKHALVLTQKALEHNPDIIVACGGDGTVNEVASCLIGTDVKLGIIPLGSGNGLASH
LNIPHEIGKSLEVIREGRKIRIDAGRINQHYFFSNTGIGIDAMIIRKYEHSGKRMLFAYVKAAVAAAFEYRAQPAIVSFD
DRVIPIRPFIVFVSNSNEMGYNMTLTPDAMLTDGKLDLVLIPELSFFEKIALGYRILTRSVSRFKKAQHHLVQSLQIEMP
MKIFTDAQIDGEHYKLRTNICRISAEPAALSVLV

Sequences:

>Translated_274_residues
MPDFYIRQFFPSDRYKIRTDYTMSRKHALVLTQKALEHNPDIIVACGGDGTVNEVASCLIGTDVKLGIIPLGSGNGLASH
LNIPHEIGKSLEVIREGRKIRIDAGRINQHYFFSNTGIGIDAMIIRKYEHSGKRMLFAYVKAAVAAAFEYRAQPAIVSFD
DRVIPIRPFIVFVSNSNEMGYNMTLTPDAMLTDGKLDLVLIPELSFFEKIALGYRILTRSVSRFKKAQHHLVQSLQIEMP
MKIFTDAQIDGEHYKLRTNICRISAEPAALSVLV
>Mature_273_residues
PDFYIRQFFPSDRYKIRTDYTMSRKHALVLTQKALEHNPDIIVACGGDGTVNEVASCLIGTDVKLGIIPLGSGNGLASHL
NIPHEIGKSLEVIREGRKIRIDAGRINQHYFFSNTGIGIDAMIIRKYEHSGKRMLFAYVKAAVAAAFEYRAQPAIVSFDD
RVIPIRPFIVFVSNSNEMGYNMTLTPDAMLTDGKLDLVLIPELSFFEKIALGYRILTRSVSRFKKAQHHLVQSLQIEMPM
KIFTDAQIDGEHYKLRTNICRISAEPAALSVLV

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=221, Percent_Identity=25.7918552036199, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 30759; Mature: 30628

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDFYIRQFFPSDRYKIRTDYTMSRKHALVLTQKALEHNPDIIVACGGDGTVNEVASCLI
CCCCEEHHCCCCCCEEEEECCEECCCEEEEEEHHHHHCCCCEEEEECCCCCHHHHHHHHH
GTDVKLGIIPLGSGNGLASHLNIPHEIGKSLEVIREGRKIRIDAGRINQHYFFSNTGIGI
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCE
DAMIIRKYEHSGKRMLFAYVKAAVAAAFEYRAQPAIVSFDDRVIPIRPFIVFVSNSNEMG
EEEEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEEEEEECCCCCC
YNMTLTPDAMLTDGKLDLVLIPELSFFEKIALGYRILTRSVSRFKKAQHHLVQSLQIEMP
EEEEECCCCEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MKIFTDAQIDGEHYKLRTNICRISAEPAALSVLV
CEEEECCEECCCEEEEEEEEEEEECCCCEEEEEC
>Mature Secondary Structure 
PDFYIRQFFPSDRYKIRTDYTMSRKHALVLTQKALEHNPDIIVACGGDGTVNEVASCLI
CCCEEHHCCCCCCEEEEECCEECCCEEEEEEHHHHHCCCCEEEEECCCCCHHHHHHHHH
GTDVKLGIIPLGSGNGLASHLNIPHEIGKSLEVIREGRKIRIDAGRINQHYFFSNTGIGI
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCE
DAMIIRKYEHSGKRMLFAYVKAAVAAAFEYRAQPAIVSFDDRVIPIRPFIVFVSNSNEMG
EEEEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEEEEEEECCCCCC
YNMTLTPDAMLTDGKLDLVLIPELSFFEKIALGYRILTRSVSRFKKAQHHLVQSLQIEMP
EEEEECCCCEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MKIFTDAQIDGEHYKLRTNICRISAEPAALSVLV
CEEEECCEECCCEEEEEEEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA