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The map label for this gene is iscS [H]

Identifier: 146300756

GI number: 146300756

Start: 3554237

End: 3555373

Strand: Direct

Name: iscS [H]

Synonym: Fjoh_3007

Alternate gene names: 146300756

Gene position: 3554237-3555373 (Clockwise)

Preceding gene: 146300747

Following gene: 146300757

Centisome position: 58.3

GC content: 40.19

Gene sequence:

>1137_bases
TTGAGAGATACTTTAATATATTTAGACAACAATGCCACTACTGCCGTTGACAATCGAGTTGTTGAGGTAATTAATATTTA
CTTCAATCAACTTTATGGCAATGCAAGCAGTCTTTATAATTTCGGATCTAAATCCAAATTAATTTTAGAACAAGCAAGAA
CCAGCATTTCTGATTTGATTGATGCTGAGCCAGAAAGCATCTATTTTACCTCGGGCGCGACTGAAAGCGTCAACCTGGCA
TTGAAGGGTCTTGCATTAAGCCCTAAAAATTCAAAAAAACACATCATAACATGCAGTACCGAGCATAAAGCCGTATTGGA
AACATGTGAGTATTTGCAAAGCATCGGATATGAAATCGATTACCTGCCTGTTAATAATGGGGGAGCCATAGACCTTGAAG
TCCTTAAATCCCTAATTAAAGAGGATACTCTTTTGGTTTGTCTAATGTGGGTCAATAATGAAACTGGTGTCATTCACCCC
ATTAATGAAATTGGGAAAATAACTAAAGCCGCGGGGGTGTATTTCGTCTGCGACGGCTCTCAGGGAGTCGGAAAACTGCC
TATCAGCGTAACAAGCTCTAATATTGACATTTTCTGCTTTTCTGGCCATAAATTGTACGCCAGTAAAGGTGTTGGAGGAA
TCTATATCGGTTCCGAAATAACCCGTAATGATAAAATACAGCCACTAATACACGGCGGTGGTCAGGAAGACAATTTGAGA
AGCGGCACGCATAATGTCCCTTTAATTGCCGGATTAGGAAAAGCATTCGAAATTGCTAGGGAAGAAATGCAGAAAAATGA
AGATCATATAGTGCGGCTCAAAACCTTACTTGAATCTGAATTGTCAAAAATGAGCCAGACTTCTATTAATGGCGTACAGG
AACCAAGAATCTTCAATACCGTAAATGTGTGCATACTCGGCCTTGACAGCGAAGTTTTTATCGGAATTAACAGTGACATT
GCCGTGAGCAACGGCTCTGCCTGCACCTCTGCATTAGTACAACCTTCTCACGTCCTTTTAGCAATGGGATTAAGCACTAA
TGATGCCATGGACAGCATACGCATTTCGATAGGTAAATATAACACCGAGAGTGAGATTCACCAACTCATCGAGCGGATCA
ATAACTTTATCAATTAA

Upstream 100 bases:

>100_bases
CATAACATAGAGCACTGGCAGTCGTAGGAATAATTTGGCATTGTGAAGCGCCCCACAACTAAAATTTATTATATTAGCAC
ACTTAACAAAATTTACTTTT

Downstream 100 bases:

>100_bases
CTAAACCGCTTTTTTGCACTTTTTAACCTAAAAATATTATTATATAACACTACTAACCAATAACTATAATGGAACTACTC
TACGTATGGATTGAGAATTT

Product: class V aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MRDTLIYLDNNATTAVDNRVVEVINIYFNQLYGNASSLYNFGSKSKLILEQARTSISDLIDAEPESIYFTSGATESVNLA
LKGLALSPKNSKKHIITCSTEHKAVLETCEYLQSIGYEIDYLPVNNGGAIDLEVLKSLIKEDTLLVCLMWVNNETGVIHP
INEIGKITKAAGVYFVCDGSQGVGKLPISVTSSNIDIFCFSGHKLYASKGVGGIYIGSEITRNDKIQPLIHGGGQEDNLR
SGTHNVPLIAGLGKAFEIAREEMQKNEDHIVRLKTLLESELSKMSQTSINGVQEPRIFNTVNVCILGLDSEVFIGINSDI
AVSNGSACTSALVQPSHVLLAMGLSTNDAMDSIRISIGKYNTESEIHQLIERINNFIN

Sequences:

>Translated_378_residues
MRDTLIYLDNNATTAVDNRVVEVINIYFNQLYGNASSLYNFGSKSKLILEQARTSISDLIDAEPESIYFTSGATESVNLA
LKGLALSPKNSKKHIITCSTEHKAVLETCEYLQSIGYEIDYLPVNNGGAIDLEVLKSLIKEDTLLVCLMWVNNETGVIHP
INEIGKITKAAGVYFVCDGSQGVGKLPISVTSSNIDIFCFSGHKLYASKGVGGIYIGSEITRNDKIQPLIHGGGQEDNLR
SGTHNVPLIAGLGKAFEIAREEMQKNEDHIVRLKTLLESELSKMSQTSINGVQEPRIFNTVNVCILGLDSEVFIGINSDI
AVSNGSACTSALVQPSHVLLAMGLSTNDAMDSIRISIGKYNTESEIHQLIERINNFIN
>Mature_378_residues
MRDTLIYLDNNATTAVDNRVVEVINIYFNQLYGNASSLYNFGSKSKLILEQARTSISDLIDAEPESIYFTSGATESVNLA
LKGLALSPKNSKKHIITCSTEHKAVLETCEYLQSIGYEIDYLPVNNGGAIDLEVLKSLIKEDTLLVCLMWVNNETGVIHP
INEIGKITKAAGVYFVCDGSQGVGKLPISVTSSNIDIFCFSGHKLYASKGVGGIYIGSEITRNDKIQPLIHGGGQEDNLR
SGTHNVPLIAGLGKAFEIAREEMQKNEDHIVRLKTLLESELSKMSQTSINGVQEPRIFNTVNVCILGLDSEVFIGINSDI
AVSNGSACTSALVQPSHVLLAMGLSTNDAMDSIRISIGKYNTESEIHQLIERINNFIN

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=369, Percent_Identity=41.7344173441734, Blast_Score=295, Evalue=4e-80,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=32.2033898305085, Blast_Score=193, Evalue=3e-49,
Organism=Escherichia coli, GI48994898, Length=375, Percent_Identity=42.6666666666667, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI1789175, Length=404, Percent_Identity=26.980198019802, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787970, Length=408, Percent_Identity=25.7352941176471, Blast_Score=108, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI25143064, Length=369, Percent_Identity=42.5474254742547, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=29.6636085626911, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319831, Length=374, Percent_Identity=41.7112299465241, Blast_Score=295, Evalue=9e-81,
Organism=Drosophila melanogaster, GI20129463, Length=363, Percent_Identity=40.771349862259, Blast_Score=276, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41176; Mature: 41176

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDTLIYLDNNATTAVDNRVVEVINIYFNQLYGNASSLYNFGSKSKLILEQARTSISDLI
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHH
DAEPESIYFTSGATESVNLALKGLALSPKNSKKHIITCSTEHKAVLETCEYLQSIGYEID
CCCCCEEEEECCCCCEEEEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEE
YLPVNNGGAIDLEVLKSLIKEDTLLVCLMWVNNETGVIHPINEIGKITKAAGVYFVCDGS
EEECCCCCEEEHHHHHHHHCCCCEEEEEEEECCCCCEEECHHHHHHHHHHCCEEEEEECC
QGVGKLPISVTSSNIDIFCFSGHKLYASKGVGGIYIGSEITRNDKIQPLIHGGGQEDNLR
CCCCCCEEEEECCCEEEEEECCCEEEECCCCCEEEECCEECCCCCCCEEEECCCCCCCCC
SGTHNVPLIAGLGKAFEIAREEMQKNEDHIVRLKTLLESELSKMSQTSINGVQEPRIFNT
CCCCCCCEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
VNVCILGLDSEVFIGINSDIAVSNGSACTSALVQPSHVLLAMGLSTNDAMDSIRISIGKY
EEEEEEECCCEEEEEECCCEEECCCCHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECC
NTESEIHQLIERINNFIN
CCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRDTLIYLDNNATTAVDNRVVEVINIYFNQLYGNASSLYNFGSKSKLILEQARTSISDLI
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHH
DAEPESIYFTSGATESVNLALKGLALSPKNSKKHIITCSTEHKAVLETCEYLQSIGYEID
CCCCCEEEEECCCCCEEEEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEE
YLPVNNGGAIDLEVLKSLIKEDTLLVCLMWVNNETGVIHPINEIGKITKAAGVYFVCDGS
EEECCCCCEEEHHHHHHHHCCCCEEEEEEEECCCCCEEECHHHHHHHHHHCCEEEEEECC
QGVGKLPISVTSSNIDIFCFSGHKLYASKGVGGIYIGSEITRNDKIQPLIHGGGQEDNLR
CCCCCCEEEEECCCEEEEEECCCEEEECCCCCEEEECCEECCCCCCCEEEECCCCCCCCC
SGTHNVPLIAGLGKAFEIAREEMQKNEDHIVRLKTLLESELSKMSQTSINGVQEPRIFNT
CCCCCCCEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
VNVCILGLDSEVFIGINSDIAVSNGSACTSALVQPSHVLLAMGLSTNDAMDSIRISIGKY
EEEEEEECCCEEEEEECCCEEECCCCHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECC
NTESEIHQLIERINNFIN
CCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA