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The map label for this gene is pcrA [H]

Identifier: 146300673

GI number: 146300673

Start: 3465224

End: 3467560

Strand: Reverse

Name: pcrA [H]

Synonym: Fjoh_2924

Alternate gene names: 146300673

Gene position: 3467560-3465224 (Counterclockwise)

Preceding gene: 146300674

Following gene: 146300672

Centisome position: 56.87

GC content: 35.9

Gene sequence:

>2337_bases
ATGCAGAAATATATTGACCAGCTCAACGAAGCGCAGAGACAGCCTGTTCTAAAAAAAGACGGGCCAATGATTATTATTGC
TGGTGCAGGTTCGGGAAAAACTCGTGTTTTAACGATTAGAATTGCTTATTTGATGGCGCAGGGAGTTGACGCCTTTAATA
TTTTATCGCTGACGTTTACTAATAAAGCGGCACGTGAAATGAAGCACAGGATTGCAGATATTGTAGGAGCATCTGAAGCA
AAAAATCTTTGGATGGGAACTTTTCACTCGATTTTTGCTCGTATTCTTCGCTCAGAATCAGATCATTTGGGGTATCCTTC
TAATTTTACTATTTATGATTCTCAGGATTCGGCCAGATTAATTTCTTCTATTATTAAAGAAATGCAGCTGGATCGTGATG
TTTATAAACCAAAACAAATTTTAGGACGTATATCTAGTTATAAAAACAGTTTGATTACTGTAAAAGCATATTTCAATAAT
CCGGAATTGGTTGAAGCCGATGCAATGGCAAAACGACCAAGATTGGGAGATATTTATCAGCAGTATGTAGAGCGTTGCTT
TAAAGCTGGTGCAATGGATTTTGACGATTTGCTGCTAAAAACCAACGAACTATTAACTAGATTTCCAGAAGTTTTAGCCA
AATATCAGGATCGTTTCCGTTACATTCTTGTTGATGAGTACCAAGATACAAATCACTCTCAGTATTTGATTGTTAGAGCT
TTATCAGACAGATTTCAGAATATTTGCGTGGTTGGAGATGATGCGCAGAGTATTTATGCTTTCCGTGGCGCGAATATTAA
TAACATCTTGAATTTCCAAAAGGATTATGAAGGTGTAGTTATGTTTCGTTTAGAGCAAAATTACCGTTCAACCCGAAATA
TTGTAGAAGCGGCAAATACGGTAATGGAACATAATAAAACTAAACTTGATAAAGTAGTTTGGACTGCGAATGAATTTGGA
CCAAAAATTAAAGTCCACAGAAGTTTGACAGATGCCGAAGAAGGCCGTTTTGTTGCCAGTACGATCTTTGAACAAAAAAT
GCAGAATCAGCTTCACAATGGATCTTTTGCTATTTTGTATCGTACAAACGCACAGTCGCGTGCTATGGAGGATGCTTTAA
GGAAAAGAGATATTCCGTATAGAATTTATGGAGGATTGTCATTTTACCAGCGTAAGGAAATTAAAGATGTTTTGTGTTAT
CTGCGTTTAGTAATTAATCCAAAAGATGAAGAAGCATTGATTCGAGTAATCAATTATCCTGCTCGTGGAATTGGAGATAC
AACAGTTGAAAAACTTACTATCGCAGCAAATCACTACAAACGTTCGATTTGGGAAGTGATGGTAAACATTGATAAAATTG
ATTTAAAACTGACTTCGGGTACAAAAAACAAACTAAAAGATTTTGTTACCATGATTCAGAGTTTTCAGGTTATTGATCAA
AATCAGGATGCATTTTATATAACAGATCATGTTGCCAAGAAAACCGGCTTAGTTCAGGAATTGAAAAAAGATGCTACTCC
GGAAGGAATGGCAAAAATTCAAAATATTGAAGAACTTTTAAACGGTATTAAAGATTTTACAGAAGGACAAAAAGAAATTG
ACGGTGCAAGAGGAGCTTTATCTGAATTTATGGAAGATGTGGCACTTGCGACGGATTTAGATAAAGATACAACAGATGAA
GATCGTGTAGCGTTAATGACCATTCACCTTGCAAAAGGACTTGAGTTTCCTCATGTTTTTGTAGTTGGAATGGAAGAAGA
TTTGTTTCCTAGTGCTATGAGTATGAGTACCAGAAGTGAACTTGAAGAAGAACGTCGTTTGTTTTATGTTGCATTAACAC
GTGCAGAACACCAAGCGTATTTGACTTATGCCCAATCTCGTTACCGCTGGGGGAAATTAACAGACAGCGAACCGTCTCGT
TTTATTGAAGAAATAGATGGACAATATTTAGAATATCTAACTCCGGCTGAAACGAATTACAGATATAAATCGCCTATCGA
TGGAGATATTTTTGGAGATATTGATAAATCAAAGTTAAGATTAGCAAAACCGGTAGGAGGAACTCCGCCAAAACATATTA
CAGATAATAATCCAAAACCGGATTTAAATATTCGTAAATTAAAACCTGTTTCAGGAAATGCGCCAGCAAGTTCAAGTGCT
AATTTGTTTGATAATAAATTAACTATTGGGAATGTTGTAATGCATGAACGTTTTGGAAAAGGAGAAGTGATTAATTTAGA
AGGTGTTGGAGCTGATAAAAAAGCCGAAATTAAATTTGAAGTAGGAGGTATTAAAAAACTCTTGCTGAGATTTGCTAAAT
TAGATGTGGTTGGGTAG

Upstream 100 bases:

>100_bases
TCTGCTTCAAAAAATAAAAACTTAGCATCTTAGAACCTTAGCATCTTAGTAGCTATTTAGAGTATTAATTCTTAATTTTG
CATAAAAGGAGAAAAATTAC

Downstream 100 bases:

>100_bases
TATGAGTAAACTATTATGCAATTTAATCTTTTTAGTTTCTTTAAGTGTTTTTGGACAATCAGAAAAATTGGTAAGAAAAA
ATGATAAAAAGTTAGTTGGA

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 778; Mature: 778

Protein sequence:

>778_residues
MQKYIDQLNEAQRQPVLKKDGPMIIIAGAGSGKTRVLTIRIAYLMAQGVDAFNILSLTFTNKAAREMKHRIADIVGASEA
KNLWMGTFHSIFARILRSESDHLGYPSNFTIYDSQDSARLISSIIKEMQLDRDVYKPKQILGRISSYKNSLITVKAYFNN
PELVEADAMAKRPRLGDIYQQYVERCFKAGAMDFDDLLLKTNELLTRFPEVLAKYQDRFRYILVDEYQDTNHSQYLIVRA
LSDRFQNICVVGDDAQSIYAFRGANINNILNFQKDYEGVVMFRLEQNYRSTRNIVEAANTVMEHNKTKLDKVVWTANEFG
PKIKVHRSLTDAEEGRFVASTIFEQKMQNQLHNGSFAILYRTNAQSRAMEDALRKRDIPYRIYGGLSFYQRKEIKDVLCY
LRLVINPKDEEALIRVINYPARGIGDTTVEKLTIAANHYKRSIWEVMVNIDKIDLKLTSGTKNKLKDFVTMIQSFQVIDQ
NQDAFYITDHVAKKTGLVQELKKDATPEGMAKIQNIEELLNGIKDFTEGQKEIDGARGALSEFMEDVALATDLDKDTTDE
DRVALMTIHLAKGLEFPHVFVVGMEEDLFPSAMSMSTRSELEEERRLFYVALTRAEHQAYLTYAQSRYRWGKLTDSEPSR
FIEEIDGQYLEYLTPAETNYRYKSPIDGDIFGDIDKSKLRLAKPVGGTPPKHITDNNPKPDLNIRKLKPVSGNAPASSSA
NLFDNKLTIGNVVMHERFGKGEVINLEGVGADKKAEIKFEVGGIKKLLLRFAKLDVVG

Sequences:

>Translated_778_residues
MQKYIDQLNEAQRQPVLKKDGPMIIIAGAGSGKTRVLTIRIAYLMAQGVDAFNILSLTFTNKAAREMKHRIADIVGASEA
KNLWMGTFHSIFARILRSESDHLGYPSNFTIYDSQDSARLISSIIKEMQLDRDVYKPKQILGRISSYKNSLITVKAYFNN
PELVEADAMAKRPRLGDIYQQYVERCFKAGAMDFDDLLLKTNELLTRFPEVLAKYQDRFRYILVDEYQDTNHSQYLIVRA
LSDRFQNICVVGDDAQSIYAFRGANINNILNFQKDYEGVVMFRLEQNYRSTRNIVEAANTVMEHNKTKLDKVVWTANEFG
PKIKVHRSLTDAEEGRFVASTIFEQKMQNQLHNGSFAILYRTNAQSRAMEDALRKRDIPYRIYGGLSFYQRKEIKDVLCY
LRLVINPKDEEALIRVINYPARGIGDTTVEKLTIAANHYKRSIWEVMVNIDKIDLKLTSGTKNKLKDFVTMIQSFQVIDQ
NQDAFYITDHVAKKTGLVQELKKDATPEGMAKIQNIEELLNGIKDFTEGQKEIDGARGALSEFMEDVALATDLDKDTTDE
DRVALMTIHLAKGLEFPHVFVVGMEEDLFPSAMSMSTRSELEEERRLFYVALTRAEHQAYLTYAQSRYRWGKLTDSEPSR
FIEEIDGQYLEYLTPAETNYRYKSPIDGDIFGDIDKSKLRLAKPVGGTPPKHITDNNPKPDLNIRKLKPVSGNAPASSSA
NLFDNKLTIGNVVMHERFGKGEVINLEGVGADKKAEIKFEVGGIKKLLLRFAKLDVVG
>Mature_778_residues
MQKYIDQLNEAQRQPVLKKDGPMIIIAGAGSGKTRVLTIRIAYLMAQGVDAFNILSLTFTNKAAREMKHRIADIVGASEA
KNLWMGTFHSIFARILRSESDHLGYPSNFTIYDSQDSARLISSIIKEMQLDRDVYKPKQILGRISSYKNSLITVKAYFNN
PELVEADAMAKRPRLGDIYQQYVERCFKAGAMDFDDLLLKTNELLTRFPEVLAKYQDRFRYILVDEYQDTNHSQYLIVRA
LSDRFQNICVVGDDAQSIYAFRGANINNILNFQKDYEGVVMFRLEQNYRSTRNIVEAANTVMEHNKTKLDKVVWTANEFG
PKIKVHRSLTDAEEGRFVASTIFEQKMQNQLHNGSFAILYRTNAQSRAMEDALRKRDIPYRIYGGLSFYQRKEIKDVLCY
LRLVINPKDEEALIRVINYPARGIGDTTVEKLTIAANHYKRSIWEVMVNIDKIDLKLTSGTKNKLKDFVTMIQSFQVIDQ
NQDAFYITDHVAKKTGLVQELKKDATPEGMAKIQNIEELLNGIKDFTEGQKEIDGARGALSEFMEDVALATDLDKDTTDE
DRVALMTIHLAKGLEFPHVFVVGMEEDLFPSAMSMSTRSELEEERRLFYVALTRAEHQAYLTYAQSRYRWGKLTDSEPSR
FIEEIDGQYLEYLTPAETNYRYKSPIDGDIFGDIDKSKLRLAKPVGGTPPKHITDNNPKPDLNIRKLKPVSGNAPASSSA
NLFDNKLTIGNVVMHERFGKGEVINLEGVGADKKAEIKFEVGGIKKLLLRFAKLDVVG

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=780, Percent_Identity=36.7948717948718, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI48994965, Length=645, Percent_Identity=35.3488372093023, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6322369, Length=741, Percent_Identity=29.0148448043185, Blast_Score=220, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6324477, Length=680, Percent_Identity=23.6764705882353, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 88604; Mature: 88604

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKYIDQLNEAQRQPVLKKDGPMIIIAGAGSGKTRVLTIRIAYLMAQGVDAFNILSLTFT
CCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEEEHHHHHCCCCCEEEEEEEEC
NKAAREMKHRIADIVGASEAKNLWMGTFHSIFARILRSESDHLGYPSNFTIYDSQDSARL
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHH
ISSIIKEMQLDRDVYKPKQILGRISSYKNSLITVKAYFNNPELVEADAMAKRPRLGDIYQ
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEECCCCEEEHHHHHCCCCCHHHHH
QYVERCFKAGAMDFDDLLLKTNELLTRFPEVLAKYQDRFRYILVDEYQDTNHSQYLIVRA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEE
LSDRFQNICVVGDDAQSIYAFRGANINNILNFQKDYEGVVMFRLEQNYRSTRNIVEAANT
HHHHHCCEEEECCCHHHEEEECCCCHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHH
VMEHNKTKLDKVVWTANEFGPKIKVHRSLTDAEEGRFVASTIFEQKMQNQLHNGSFAILY
HHHCCHHHHHHHEEEHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
RTNAQSRAMEDALRKRDIPYRIYGGLSFYQRKEIKDVLCYLRLVINPKDEEALIRVINYP
ECCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
ARGIGDTTVEKLTIAANHYKRSIWEVMVNIDKIDLKLTSGTKNKLKDFVTMIQSFQVIDQ
CCCCCCHHHHHEEEHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCC
NQDAFYITDHVAKKTGLVQELKKDATPEGMAKIQNIEELLNGIKDFTEGQKEIDGARGAL
CCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHH
SEFMEDVALATDLDKDTTDEDRVALMTIHLAKGLEFPHVFVVGMEEDLFPSAMSMSTRSE
HHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHCCCCCCEEEEEECCCCCCCHHHHHHHHHH
LEEERRLFYVALTRAEHQAYLTYAQSRYRWGKLTDSEPSRFIEEIDGQYLEYLTPAETNY
HHHHHCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCC
RYKSPIDGDIFGDIDKSKLRLAKPVGGTPPKHITDNNPKPDLNIRKLKPVSGNAPASSSA
EECCCCCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCC
NLFDNKLTIGNVVMHERFGKGEVINLEGVGADKKAEIKFEVGGIKKLLLRFAKLDVVG
CCCCCCEEECHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQKYIDQLNEAQRQPVLKKDGPMIIIAGAGSGKTRVLTIRIAYLMAQGVDAFNILSLTFT
CCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEEEHHHHHCCCCCEEEEEEEEC
NKAAREMKHRIADIVGASEAKNLWMGTFHSIFARILRSESDHLGYPSNFTIYDSQDSARL
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHH
ISSIIKEMQLDRDVYKPKQILGRISSYKNSLITVKAYFNNPELVEADAMAKRPRLGDIYQ
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEECCCCEEEHHHHHCCCCCHHHHH
QYVERCFKAGAMDFDDLLLKTNELLTRFPEVLAKYQDRFRYILVDEYQDTNHSQYLIVRA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEE
LSDRFQNICVVGDDAQSIYAFRGANINNILNFQKDYEGVVMFRLEQNYRSTRNIVEAANT
HHHHHCCEEEECCCHHHEEEECCCCHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHH
VMEHNKTKLDKVVWTANEFGPKIKVHRSLTDAEEGRFVASTIFEQKMQNQLHNGSFAILY
HHHCCHHHHHHHEEEHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
RTNAQSRAMEDALRKRDIPYRIYGGLSFYQRKEIKDVLCYLRLVINPKDEEALIRVINYP
ECCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
ARGIGDTTVEKLTIAANHYKRSIWEVMVNIDKIDLKLTSGTKNKLKDFVTMIQSFQVIDQ
CCCCCCHHHHHEEEHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCC
NQDAFYITDHVAKKTGLVQELKKDATPEGMAKIQNIEELLNGIKDFTEGQKEIDGARGAL
CCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHH
SEFMEDVALATDLDKDTTDEDRVALMTIHLAKGLEFPHVFVVGMEEDLFPSAMSMSTRSE
HHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHCCCCCCEEEEEECCCCCCCHHHHHHHHHH
LEEERRLFYVALTRAEHQAYLTYAQSRYRWGKLTDSEPSRFIEEIDGQYLEYLTPAETNY
HHHHHCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCC
RYKSPIDGDIFGDIDKSKLRLAKPVGGTPPKHITDNNPKPDLNIRKLKPVSGNAPASSSA
EECCCCCCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCC
NLFDNKLTIGNVVMHERFGKGEVINLEGVGADKKAEIKFEVGGIKKLLLRFAKLDVVG
CCCCCCEEECHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]