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The map label for this gene is manA [H]

Identifier: 146300479

GI number: 146300479

Start: 3251283

End: 3252434

Strand: Direct

Name: manA [H]

Synonym: Fjoh_2729

Alternate gene names: 146300479

Gene position: 3251283-3252434 (Clockwise)

Preceding gene: 146300478

Following gene: 146300480

Centisome position: 53.33

GC content: 33.07

Gene sequence:

>1152_bases
ATGAAAACTAACTTAAATAAAACAAATGCCTTGTTATCTCTATTTATGATAACAGGGCATTTTCTTTTTGCACAAATTGA
TAAAAGCGCAACAAGAGAAACAAAAAACTTATATCGCAATCTAAAAAGTATTTCAAAAGAGCATATCTTATTTGGGCATC
AACACGCTTTAGAATACGGACACGGCTGGTCAAACGAACCTAATAAATCGGATGTTAAATTGGTTACAGGTTCACATCCT
GCAGTTGTAGGAATAGATTTCAGCGATTTTTCGGGACGGTCAAGAGAACAAATTAAAAAAGCAAAAGAAACTTTAAGAAA
AAATGTAATTGATACTTACGAGCGAGGCGGTATCACAACGGTTTCCTGGCATTTCAATAATCCTGCTTCGGGTGGTGGAT
TTTACTGGAAAGATTCTATTTCAGCCCCATCCATGTCTTTAATAAAAAAAGGAGGTTCGCATCATGAGCAATACAAAGAA
ATTTTAAAAACAATCGCTGACTTTGCAAATTCTGCTAAAGCAAAAGATGGTAAATTGGTTCCAATGATTTTTAGACCTTT
TCATGAATTTGATGGTGATTGGTTCTGGTGGGGAAAAAAATATACATCGCGTGAGGATTTTATAGAAGTCTGGCAATTTA
CTGTGTCATACCTGAGAGATACTTTAAAAGTTCATAATTTTATTTACGCATTTTCACCAGATAATCGATTTAATTCTGAG
TCAGAATATTTAGAACGCTATCCTGGCGATGATTATGTTGATATGTTTGGGATAGATAATTATGGAGATTTTGGACGTGA
CGGGAAATATGACTTAGAAAACGGAATAAAAAAGCTCAAAATCATTTCTGACATTGCAATTAAAAAGAACAAATTAGCAG
CTTTTACAGAAACCGGCTTAGAATCAATAACAAATAAAAACTGGTGGACAGAAGTTTTACTTCAATCTATAAAGAAAGAA
AATATTAAGCTTTGTTATGTCTTAGTCTGGCGAAATGATGCAGTAAGTTTAACGCATTATTATGCACCTTTTCCGGGGCA
GATTAGTGTTCCTGATTTTCTTCAATTTTATAATGATCCGTATACTTTATTCGAAAACGATTTGATAAATGTATATGGAA
CTAAATTTTCATTAAAAAACACCGCAAAATAG

Upstream 100 bases:

>100_bases
AAGCTGATATTGACAATATTGATCAACGTATTGAAAAAAACGTATTACAATACCTTACTTCTAAATAGTAATTTTCGGGT
TTAAACTAACTATTTAAACC

Downstream 100 bases:

>100_bases
CTTAAAGAATACATGTTAAAATGATTAAATGAGTGATTACTATTTCGATGTAAAATAGGTATTTTCTTTCTTTTTGTGTT
TTTTACTTACATTGATAATT

Product: Mannan endo-1,4-beta-mannosidase

Products: NA

Alternate protein names: Mannanase A [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MKTNLNKTNALLSLFMITGHFLFAQIDKSATRETKNLYRNLKSISKEHILFGHQHALEYGHGWSNEPNKSDVKLVTGSHP
AVVGIDFSDFSGRSREQIKKAKETLRKNVIDTYERGGITTVSWHFNNPASGGGFYWKDSISAPSMSLIKKGGSHHEQYKE
ILKTIADFANSAKAKDGKLVPMIFRPFHEFDGDWFWWGKKYTSREDFIEVWQFTVSYLRDTLKVHNFIYAFSPDNRFNSE
SEYLERYPGDDYVDMFGIDNYGDFGRDGKYDLENGIKKLKIISDIAIKKNKLAAFTETGLESITNKNWWTEVLLQSIKKE
NIKLCYVLVWRNDAVSLTHYYAPFPGQISVPDFLQFYNDPYTLFENDLINVYGTKFSLKNTAK

Sequences:

>Translated_383_residues
MKTNLNKTNALLSLFMITGHFLFAQIDKSATRETKNLYRNLKSISKEHILFGHQHALEYGHGWSNEPNKSDVKLVTGSHP
AVVGIDFSDFSGRSREQIKKAKETLRKNVIDTYERGGITTVSWHFNNPASGGGFYWKDSISAPSMSLIKKGGSHHEQYKE
ILKTIADFANSAKAKDGKLVPMIFRPFHEFDGDWFWWGKKYTSREDFIEVWQFTVSYLRDTLKVHNFIYAFSPDNRFNSE
SEYLERYPGDDYVDMFGIDNYGDFGRDGKYDLENGIKKLKIISDIAIKKNKLAAFTETGLESITNKNWWTEVLLQSIKKE
NIKLCYVLVWRNDAVSLTHYYAPFPGQISVPDFLQFYNDPYTLFENDLINVYGTKFSLKNTAK
>Mature_383_residues
MKTNLNKTNALLSLFMITGHFLFAQIDKSATRETKNLYRNLKSISKEHILFGHQHALEYGHGWSNEPNKSDVKLVTGSHP
AVVGIDFSDFSGRSREQIKKAKETLRKNVIDTYERGGITTVSWHFNNPASGGGFYWKDSISAPSMSLIKKGGSHHEQYKE
ILKTIADFANSAKAKDGKLVPMIFRPFHEFDGDWFWWGKKYTSREDFIEVWQFTVSYLRDTLKVHNFIYAFSPDNRFNSE
SEYLERYPGDDYVDMFGIDNYGDFGRDGKYDLENGIKKLKIISDIAIKKNKLAAFTETGLESITNKNWWTEVLLQSIKKE
NIKLCYVLVWRNDAVSLTHYYAPFPGQISVPDFLQFYNDPYTLFENDLINVYGTKFSLKNTAK

Specific function: Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. Preferentially hydrolyzes the

COG id: COG4124

COG function: function code G; Beta-mannanase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 26 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022790
- InterPro:   IPR000805
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02156 Glyco_hydro_26 [H]

EC number: =3.2.1.78 [H]

Molecular weight: Translated: 44222; Mature: 44222

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNLNKTNALLSLFMITGHFLFAQIDKSATRETKNLYRNLKSISKEHILFGHQHALEYG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHC
HGWSNEPNKSDVKLVTGSHPAVVGIDFSDFSGRSREQIKKAKETLRKNVIDTYERGGITT
CCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
VSWHFNNPASGGGFYWKDSISAPSMSLIKKGGSHHEQYKEILKTIADFANSAKAKDGKLV
EEEECCCCCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEE
PMIFRPFHEFDGDWFWWGKKYTSREDFIEVWQFTVSYLRDTLKVHNFIYAFSPDNRFNSE
HHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCH
SEYLERYPGDDYVDMFGIDNYGDFGRDGKYDLENGIKKLKIISDIAIKKNKLAAFTETGL
HHHHHHCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ESITNKNWWTEVLLQSIKKENIKLCYVLVWRNDAVSLTHYYAPFPGQISVPDFLQFYNDP
HHHCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHCCC
YTLFENDLINVYGTKFSLKNTAK
CEEHHCCEEEEECCEEECCCCCC
>Mature Secondary Structure
MKTNLNKTNALLSLFMITGHFLFAQIDKSATRETKNLYRNLKSISKEHILFGHQHALEYG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHC
HGWSNEPNKSDVKLVTGSHPAVVGIDFSDFSGRSREQIKKAKETLRKNVIDTYERGGITT
CCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
VSWHFNNPASGGGFYWKDSISAPSMSLIKKGGSHHEQYKEILKTIADFANSAKAKDGKLV
EEEECCCCCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEE
PMIFRPFHEFDGDWFWWGKKYTSREDFIEVWQFTVSYLRDTLKVHNFIYAFSPDNRFNSE
HHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCH
SEYLERYPGDDYVDMFGIDNYGDFGRDGKYDLENGIKKLKIISDIAIKKNKLAAFTETGL
HHHHHHCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ESITNKNWWTEVLLQSIKKENIKLCYVLVWRNDAVSLTHYYAPFPGQISVPDFLQFYNDP
HHHCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHCCC
YTLFENDLINVYGTKFSLKNTAK
CEEHHCCEEEEECCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7848261 [H]