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The map label for this gene is mscS [H]

Identifier: 146300382

GI number: 146300382

Start: 3122140

End: 3122976

Strand: Reverse

Name: mscS [H]

Synonym: Fjoh_2627

Alternate gene names: 146300382

Gene position: 3122976-3122140 (Counterclockwise)

Preceding gene: 146300384

Following gene: 146300381

Centisome position: 51.22

GC content: 34.17

Gene sequence:

>837_bases
ATGTTCGACTTTGCAAAAGACTTTAGAATAGGCATCATTATTTTTATAATAGCCATTACAACCATTATTCTGGGAGCAGT
AACAGACAAATTGTTACGTTATTTTTTGTACCGAAAACTAACTAATAAAGAATATGATGCCACGGGATTTAAGTTCTTAA
AACATTTAATTATAACTGTAATTTATATTCTGGGTTTTGCTTTTGCTTTAATTCAAATTCCAGAATTTAAAATTATCGGG
CATTCGTTTTTAGCGGGAGCAGGAGTTATTTCATTAGTTGCCGGTCTTGCTTCGCAACAGGCTTTGAGTAATATTGTGAG
CGGTATTTTTTTGGTGATTTTTAAACCTTTTAGAATTAACGATAAAATCACGATCAACAATTTTGTAGGTACTGTAGAAG
ATATCAACCTAAGGCAGGTCGTTTTAAAAGATGCCGAAAACAATCGAATTATAATTCCAAACTCTGTTATCAGCGCTCAA
ATTATTGTAAATACCAATATGCATGACACCAAATGCTGTAAAATAATCGAAATAGGTATTGGTTACGAATCTGATGTTGA
AAAAGCCCTTGAAATAATGCAAGATGAAATTGCTAAACATCCTCTTTTTATTGATACCAGAACTGCCGAAAATAAAAAAG
CAAAAACATCTTTGGTGACAGCAAGAGTTGTGGCTCTGGCAGATTCGAGCGTAACTTTAAAAGCATGGGCCTGGGCAAAA
AATTCTACAGATGGTTTTGTGATGTACTGCGATTTACTGCAAAGTATCAAGAAACGTTTTGATGAATCAGGAATCGACAT
TCCCTATCCACAGCGGGTTGTAACGCTTAAAAAGTAG

Upstream 100 bases:

>100_bases
CAATTCAGTTATTTCAATCTTAAAATTATTACTTTGATATCGGGTATTTTCGCTAATAACAATAACTTTGTTAAGCATCA
ATTTAAAATAGTAAAAAGGT

Downstream 100 bases:

>100_bases
ATTCACATTTTTTCTGATAATTTTAAGATTTAAATTCTTGCTTTTAAAAATGCATGACGTAATTTTATAATTCTTAACCC
AAAAAAAGCGTCAAAATGAA

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MFDFAKDFRIGIIIFIIAITTIILGAVTDKLLRYFLYRKLTNKEYDATGFKFLKHLIITVIYILGFAFALIQIPEFKIIG
HSFLAGAGVISLVAGLASQQALSNIVSGIFLVIFKPFRINDKITINNFVGTVEDINLRQVVLKDAENNRIIIPNSVISAQ
IIVNTNMHDTKCCKIIEIGIGYESDVEKALEIMQDEIAKHPLFIDTRTAENKKAKTSLVTARVVALADSSVTLKAWAWAK
NSTDGFVMYCDLLQSIKKRFDESGIDIPYPQRVVTLKK

Sequences:

>Translated_278_residues
MFDFAKDFRIGIIIFIIAITTIILGAVTDKLLRYFLYRKLTNKEYDATGFKFLKHLIITVIYILGFAFALIQIPEFKIIG
HSFLAGAGVISLVAGLASQQALSNIVSGIFLVIFKPFRINDKITINNFVGTVEDINLRQVVLKDAENNRIIIPNSVISAQ
IIVNTNMHDTKCCKIIEIGIGYESDVEKALEIMQDEIAKHPLFIDTRTAENKKAKTSLVTARVVALADSSVTLKAWAWAK
NSTDGFVMYCDLLQSIKKRFDESGIDIPYPQRVVTLKK
>Mature_278_residues
MFDFAKDFRIGIIIFIIAITTIILGAVTDKLLRYFLYRKLTNKEYDATGFKFLKHLIITVIYILGFAFALIQIPEFKIIG
HSFLAGAGVISLVAGLASQQALSNIVSGIFLVIFKPFRINDKITINNFVGTVEDINLRQVVLKDAENNRIIIPNSVISAQ
IIVNTNMHDTKCCKIIEIGIGYESDVEKALEIMQDEIAKHPLFIDTRTAENKKAKTSLVTARVVALADSSVTLKAWAWAK
NSTDGFVMYCDLLQSIKKRFDESGIDIPYPQRVVTLKK

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=264, Percent_Identity=30.3030303030303, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786670, Length=115, Percent_Identity=32.1739130434783, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI2367355, Length=267, Percent_Identity=23.5955056179775, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 31137; Mature: 31137

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDFAKDFRIGIIIFIIAITTIILGAVTDKLLRYFLYRKLTNKEYDATGFKFLKHLIITV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IYILGFAFALIQIPEFKIIGHSFLAGAGVISLVAGLASQQALSNIVSGIFLVIFKPFRIN
HHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECC
DKITINNFVGTVEDINLRQVVLKDAENNRIIIPNSVISAQIIVNTNMHDTKCCKIIEIGI
CEEEECCCCCCCCCCCHHHHHEECCCCCEEEEECCCEEEEEEEECCCCCHHHEEEEEECC
GYESDVEKALEIMQDEIAKHPLFIDTRTAENKKAKTSLVTARVVALADSSVTLKAWAWAK
CCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHEEECCCEEEEEEEECC
NSTDGFVMYCDLLQSIKKRFDESGIDIPYPQRVVTLKK
CCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MFDFAKDFRIGIIIFIIAITTIILGAVTDKLLRYFLYRKLTNKEYDATGFKFLKHLIITV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IYILGFAFALIQIPEFKIIGHSFLAGAGVISLVAGLASQQALSNIVSGIFLVIFKPFRIN
HHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECC
DKITINNFVGTVEDINLRQVVLKDAENNRIIIPNSVISAQIIVNTNMHDTKCCKIIEIGI
CEEEECCCCCCCCCCCHHHHHEECCCCCEEEEECCCEEEEEEEECCCCCHHHEEEEEECC
GYESDVEKALEIMQDEIAKHPLFIDTRTAENKKAKTSLVTARVVALADSSVTLKAWAWAK
CCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHEEECCCEEEEEEEECC
NSTDGFVMYCDLLQSIKKRFDESGIDIPYPQRVVTLKK
CCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]