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The map label for this gene is ppsA [C]

Identifier: 146300142

GI number: 146300142

Start: 2861026

End: 2862903

Strand: Reverse

Name: ppsA [C]

Synonym: Fjoh_2387

Alternate gene names: 146300142

Gene position: 2862903-2861026 (Counterclockwise)

Preceding gene: 146300147

Following gene: 146300131

Centisome position: 46.96

GC content: 30.99

Gene sequence:

>1878_bases
ATGAAAAAATACATTTACAGCTTGTTTTTCTTTCTCATTTTTATGTCTTTAAGTGCGCAGAGATATTCTTCATCATTAGA
AAATTACGAAGCATATAAGGCATTTAGAGGAAAACCTTTGTCTGATAAATTCTCGAATATCGTATCTGTAAAAGTAATTT
ACGATTTACGAAAACAGAAAATGTATTATTTCAACAGCACCTTAATTCCACTTCATTATGATTTCGTAATTAATTATCTG
CATTACAATCAGGATTTAGAAGTTTTTAATAATGATAATTACAGCAATACTCAAAAAAACCGCGATTTTTTACTTGGAAA
TCTAAATCATATTAAAGGAACTGATAAATGGATTTTTGAATTGGCAGCTTCAGATCACATGCCTGTTGAACTTATAGAAC
GATTTTTCAATCTGGTTAAAAAATCAACTTTTATTGGCGAAAACCTAAAGTTTTATCTCAATAATCCAGAACAAATGGAA
TGGTTTCGTTTGGAACAGTTTAAAATACCTTGTGTAAAATCAGATTATATTTTTAATGAAATACAATATCAGGAAGTCGT
TTCCGGAACCAATATTGGTATTTTAAGACAATACAAAATCAAGGATTTAGAAAAAGTAAAACCTAATCCGGATGAAATTG
TTGTTTTAGACGGAACGCCGGATATTTTGCCAAACGTAAGAGGAATTATAGTCAACGAACTCCAAACGCCGCTAAGTCAT
TTGGTGCTTTTAGGCAAAAACAGAAAAATTCCAATTATGGCATATACAATGGCCTTAAAAGATGAAAACATAAAGAAACT
AATTTCTAAAAAAGTAGAATTAAAAATCAAGTCTGATACTTTTTTTATTAAAGAAACTGATAAAAAAATAGTCCTTAAAA
CGAATTCAAAAAAGAAAAAACTCACTATTGATAACACGGTTACAGATTTAGTAGATTTATCTAAAATTCCAAAAAAAGGA
GTAAATTATATTGGATCTAAGGCGCAGAACATGTCGTATTTAATTGCGATATCTAAAGAAATTCCGTTTAGAACTCCAGA
AGATGCACATGCCATTCCGTTTTATTTTTATACAAAACATATTCAAAAAGAATCGATTTCGCCTCTAATAAAGGAGCTTT
TGGCATTTCCTAATAAAGATTCTACGGTTTGGATTAATCAGCAGTTAAAAAAAATACGCGATGCCATTAAAAAAGAACCT
GTAGATCCTGAATTAATTTCTAAACTGAATCTGGCTTTTAAAAATGCTAAATTCAAAAATTTCAGATTCCGCTCGTCAAC
AAATGCGGAAGATTTGGATGATTTTAACGGGGCAGGTTTATATGATTCTAAAACCGGAATTTTAGGCGACAGTATTAAAA
CATTTGAAAAAGCAATTAAACAAGTCTGGGCAAGTGTCTGGAATGAAGCTTCATATAATGAAAGAGAACTATTTGGAATC
GATCAGCAGAATATTGCAATGGGTGTTTTGGTTCACCGTTCTTTTCCAGATGAACTGGCAAATGGCGTTGTGATTACAAA
GAATATTTTCAGACAAAATTTTCCAGGAATTACAGTAAATGTTCAAAAAGGAGAAAACTCAGTTGTAAAGCCGGAAAAAG
GTGAAATATGCGAACAGTTTGTGGCTTATCACTTTAATTCCGGAACTAACGATGATGATTTTGATGTTGATTATACTTCA
AACTCAAATCTAAATGACAATGAACCTTTGCTAAGCAGAAAAGAAATGAGCCGGCTTTTTATGGTCAGTAAAAAAATTGA
AGAAAAGATGTATCGTTATTGGCGAAAAAGTCTATTTCATCCTGTAGATATCGAATTTAAAATTGTGGGAGAAAATCGGG
ATTTATACATTAAACAGGTTCGCCCTTTTAATGATTAG

Upstream 100 bases:

>100_bases
TATTTTAGAAAATCGTAATGGTTTTGATTGGTTTATATAATCCGGTGGTAGATAAAACTTATGGTTTAAATACTTACTTT
TAATATTCAATAAAATTGTC

Downstream 100 bases:

>100_bases
GTACAATTGACATTTAGGCTATCGGTGGCGAAGAATAAATTTATTCTGCCACAGATTAAGGGATTATAAATTATTTAAAA
ATATGCTGGCAAACTCTCGC

Product: pyruvate phosphate dikinase

Products: AMP; H+; Phosphoenolpyruvate; Phosphate [C]

Alternate protein names: None

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MKKYIYSLFFFLIFMSLSAQRYSSSLENYEAYKAFRGKPLSDKFSNIVSVKVIYDLRKQKMYYFNSTLIPLHYDFVINYL
HYNQDLEVFNNDNYSNTQKNRDFLLGNLNHIKGTDKWIFELAASDHMPVELIERFFNLVKKSTFIGENLKFYLNNPEQME
WFRLEQFKIPCVKSDYIFNEIQYQEVVSGTNIGILRQYKIKDLEKVKPNPDEIVVLDGTPDILPNVRGIIVNELQTPLSH
LVLLGKNRKIPIMAYTMALKDENIKKLISKKVELKIKSDTFFIKETDKKIVLKTNSKKKKLTIDNTVTDLVDLSKIPKKG
VNYIGSKAQNMSYLIAISKEIPFRTPEDAHAIPFYFYTKHIQKESISPLIKELLAFPNKDSTVWINQQLKKIRDAIKKEP
VDPELISKLNLAFKNAKFKNFRFRSSTNAEDLDDFNGAGLYDSKTGILGDSIKTFEKAIKQVWASVWNEASYNERELFGI
DQQNIAMGVLVHRSFPDELANGVVITKNIFRQNFPGITVNVQKGENSVVKPEKGEICEQFVAYHFNSGTNDDDFDVDYTS
NSNLNDNEPLLSRKEMSRLFMVSKKIEEKMYRYWRKSLFHPVDIEFKIVGENRDLYIKQVRPFND

Sequences:

>Translated_625_residues
MKKYIYSLFFFLIFMSLSAQRYSSSLENYEAYKAFRGKPLSDKFSNIVSVKVIYDLRKQKMYYFNSTLIPLHYDFVINYL
HYNQDLEVFNNDNYSNTQKNRDFLLGNLNHIKGTDKWIFELAASDHMPVELIERFFNLVKKSTFIGENLKFYLNNPEQME
WFRLEQFKIPCVKSDYIFNEIQYQEVVSGTNIGILRQYKIKDLEKVKPNPDEIVVLDGTPDILPNVRGIIVNELQTPLSH
LVLLGKNRKIPIMAYTMALKDENIKKLISKKVELKIKSDTFFIKETDKKIVLKTNSKKKKLTIDNTVTDLVDLSKIPKKG
VNYIGSKAQNMSYLIAISKEIPFRTPEDAHAIPFYFYTKHIQKESISPLIKELLAFPNKDSTVWINQQLKKIRDAIKKEP
VDPELISKLNLAFKNAKFKNFRFRSSTNAEDLDDFNGAGLYDSKTGILGDSIKTFEKAIKQVWASVWNEASYNERELFGI
DQQNIAMGVLVHRSFPDELANGVVITKNIFRQNFPGITVNVQKGENSVVKPEKGEICEQFVAYHFNSGTNDDDFDVDYTS
NSNLNDNEPLLSRKEMSRLFMVSKKIEEKMYRYWRKSLFHPVDIEFKIVGENRDLYIKQVRPFND
>Mature_625_residues
MKKYIYSLFFFLIFMSLSAQRYSSSLENYEAYKAFRGKPLSDKFSNIVSVKVIYDLRKQKMYYFNSTLIPLHYDFVINYL
HYNQDLEVFNNDNYSNTQKNRDFLLGNLNHIKGTDKWIFELAASDHMPVELIERFFNLVKKSTFIGENLKFYLNNPEQME
WFRLEQFKIPCVKSDYIFNEIQYQEVVSGTNIGILRQYKIKDLEKVKPNPDEIVVLDGTPDILPNVRGIIVNELQTPLSH
LVLLGKNRKIPIMAYTMALKDENIKKLISKKVELKIKSDTFFIKETDKKIVLKTNSKKKKLTIDNTVTDLVDLSKIPKKG
VNYIGSKAQNMSYLIAISKEIPFRTPEDAHAIPFYFYTKHIQKESISPLIKELLAFPNKDSTVWINQQLKKIRDAIKKEP
VDPELISKLNLAFKNAKFKNFRFRSSTNAEDLDDFNGAGLYDSKTGILGDSIKTFEKAIKQVWASVWNEASYNERELFGI
DQQNIAMGVLVHRSFPDELANGVVITKNIFRQNFPGITVNVQKGENSVVKPEKGEICEQFVAYHFNSGTNDDDFDVDYTS
NSNLNDNEPLLSRKEMSRLFMVSKKIEEKMYRYWRKSLFHPVDIEFKIVGENRDLYIKQVRPFND

Specific function: ESSENTIAL STEP IN GLUCONEOGENESIS WHEN PYRUVATE AND LACTATE ARE USED AS A CARBON SOURCE. [C]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.9.2 [C]

Molecular weight: Translated: 72880; Mature: 72880

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYIYSLFFFLIFMSLSAQRYSSSLENYEAYKAFRGKPLSDKFSNIVSVKVIYDLRKQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEEHHHHHHHHHE
MYYFNSTLIPLHYDFVINYLHYNQDLEVFNNDNYSNTQKNRDFLLGNLNHIKGTDKWIFE
EEEECCEEEEEEHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEECCHHCCCCHHHHHE
LAASDHMPVELIERFFNLVKKSTFIGENLKFYLNNPEQMEWFRLEQFKIPCVKSDYIFNE
ECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHEEHHHEECCCCCCCHHHHH
IQYQEVVSGTNIGILRQYKIKDLEKVKPNPDEIVVLDGTPDILPNVRGIIVNELQTPLSH
HHHHHHHCCCCEEEEEEECHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHH
LVLLGKNRKIPIMAYTMALKDENIKKLISKKVELKIKSDTFFIKETDKKIVLKTNSKKKK
HEEECCCCCCEEEEEEEECCCHHHHHHHCCCEEEEEECCEEEEEECCCEEEEEECCCCCE
LTIDNTVTDLVDLSKIPKKGVNYIGSKAQNMSYLIAISKEIPFRTPEDAHAIPFYFYTKH
EEECCHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHH
IQKESISPLIKELLAFPNKDSTVWINQQLKKIRDAIKKEPVDPELISKLNLAFKNAKFKN
HHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
FRFRSSTNAEDLDDFNGAGLYDSKTGILGDSIKTFEKAIKQVWASVWNEASYNERELFGI
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECC
DQQNIAMGVLVHRSFPDELANGVVITKNIFRQNFPGITVNVQKGENSVVKPEKGEICEQF
CCCCCEEEEEEECCCCHHHHCCEEEEHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHH
VAYHFNSGTNDDDFDVDYTSNSNLNDNEPLLSRKEMSRLFMVSKKIEEKMYRYWRKSLFH
HHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVDIEFKIVGENRDLYIKQVRPFND
CCCEEEEEEECCCCEEEEECCCCCC
>Mature Secondary Structure
MKKYIYSLFFFLIFMSLSAQRYSSSLENYEAYKAFRGKPLSDKFSNIVSVKVIYDLRKQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEEHHHHHHHHHE
MYYFNSTLIPLHYDFVINYLHYNQDLEVFNNDNYSNTQKNRDFLLGNLNHIKGTDKWIFE
EEEECCEEEEEEHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEECCHHCCCCHHHHHE
LAASDHMPVELIERFFNLVKKSTFIGENLKFYLNNPEQMEWFRLEQFKIPCVKSDYIFNE
ECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHEEHHHEECCCCCCCHHHHH
IQYQEVVSGTNIGILRQYKIKDLEKVKPNPDEIVVLDGTPDILPNVRGIIVNELQTPLSH
HHHHHHHCCCCEEEEEEECHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHH
LVLLGKNRKIPIMAYTMALKDENIKKLISKKVELKIKSDTFFIKETDKKIVLKTNSKKKK
HEEECCCCCCEEEEEEEECCCHHHHHHHCCCEEEEEECCEEEEEECCCEEEEEECCCCCE
LTIDNTVTDLVDLSKIPKKGVNYIGSKAQNMSYLIAISKEIPFRTPEDAHAIPFYFYTKH
EEECCHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHH
IQKESISPLIKELLAFPNKDSTVWINQQLKKIRDAIKKEPVDPELISKLNLAFKNAKFKN
HHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
FRFRSSTNAEDLDDFNGAGLYDSKTGILGDSIKTFEKAIKQVWASVWNEASYNERELFGI
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECC
DQQNIAMGVLVHRSFPDELANGVVITKNIFRQNFPGITVNVQKGENSVVKPEKGEICEQF
CCCCCEEEEEEECCCCHHHHCCEEEEHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHH
VAYHFNSGTNDDDFDVDYTSNSNLNDNEPLLSRKEMSRLFMVSKKIEEKMYRYWRKSLFH
HHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVDIEFKIVGENRDLYIKQVRPFND
CCCEEEEEEECCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10.5 {phosphate}} 0.083 {pyruvate}} 0.028 {ATP}} [C]

Substrates: ATP; H2O; Pyruvate [C]

Specific reaction: ATP + H2O + Pyruvate --> AMP + (2) H+ + Phosphoenolpyruvate + Phosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA