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The map label for this gene is purU [H]

Identifier: 146300081

GI number: 146300081

Start: 2795152

End: 2796006

Strand: Reverse

Name: purU [H]

Synonym: Fjoh_2326

Alternate gene names: 146300081

Gene position: 2796006-2795152 (Counterclockwise)

Preceding gene: 146300082

Following gene: 146300080

Centisome position: 45.86

GC content: 34.5

Gene sequence:

>855_bases
ATGCAAAAAATAACCATTCTTATTCACTGTAAAGACCAAAAAGGTATTATTGCTGCAGTGACTACTTTTATTGCGAGAGT
AGAAGGAAATATTATGTACATCGACCAGCACGTTGATGTGGAGCAAAATGTATTTTTTATGCGATTGGAATGCGAATTCG
CCAATCATAAAATTACCATTGAAAGCTTAAAAGAAGAATTTGATAAAACCCTCGCCGCTGATTTTAATATGTCATGGGAT
TTGTACAATCAGGAACAAAAACCAAAAATGGCATTGTTTGTTTCTAAATACGATCATTGCTTGTTTGATATTTTAGGAAG
ATACAGCGCGGGCGAATTAAATGTAGAAATTCCGGTTATCATAAGTAATCATAATGATTTAAGATCGATTGCAGAACGTT
TTGACATTCCGTTTCATTGTGTGCCTTTTACAAAAGACAATAAGGAAGAAGGTGAAGCCAAACAAATTGAACTATTAAAG
CGATATGAAATCAACTTTATTGTTTTGGCGCGCTATATGCAGATTATTACGCCAAAATTGATTGAACTTTACGAAAATAG
AATTATAAATATCCACCATTCATTTTTACCTGCTTTTCCGGGTGCTAAGCCCTATCACTCAGCTTTTAAACGCGGAGTAA
AAATTATTGGGGCAACAAGCCACTACGTAACCGAAGAATTAGACGAAGGCCCAATTATTGAACAGGATATTGCCAGAGTT
TCTCATATTCACTCTGTTGAAGATTTTATCATGAAAGGCCGTGACTTAGAACGTATCGTTCTGGCAAGAGCTATAAAATT
ACATTCTGAGAGAAAAACAATGGTTTACAGTAATAAGACAGTTGTTTTTTCTTAA

Upstream 100 bases:

>100_bases
TTTTTTTCTGAAGTTACAAAGGAACAAAGTTACAAAGGGGCAGAGAAACCTTTGTAACTCTGAATCTTTGCAACTTTGTC
ACTAACCAAAATAACCAAAA

Downstream 100 bases:

>100_bases
AGGTTCAAAGGGACAAAGGTTTTTCTTTACCTAAAAAGACTTCGACTTCGCTCAGTCTGACAATAAAATTAAACCCGACA
GGTTTTTAAAACCTGTCGGG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MQKITILIHCKDQKGIIAAVTTFIARVEGNIMYIDQHVDVEQNVFFMRLECEFANHKITIESLKEEFDKTLAADFNMSWD
LYNQEQKPKMALFVSKYDHCLFDILGRYSAGELNVEIPVIISNHNDLRSIAERFDIPFHCVPFTKDNKEEGEAKQIELLK
RYEINFIVLARYMQIITPKLIELYENRIINIHHSFLPAFPGAKPYHSAFKRGVKIIGATSHYVTEELDEGPIIEQDIARV
SHIHSVEDFIMKGRDLERIVLARAIKLHSERKTMVYSNKTVVFS

Sequences:

>Translated_284_residues
MQKITILIHCKDQKGIIAAVTTFIARVEGNIMYIDQHVDVEQNVFFMRLECEFANHKITIESLKEEFDKTLAADFNMSWD
LYNQEQKPKMALFVSKYDHCLFDILGRYSAGELNVEIPVIISNHNDLRSIAERFDIPFHCVPFTKDNKEEGEAKQIELLK
RYEINFIVLARYMQIITPKLIELYENRIINIHHSFLPAFPGAKPYHSAFKRGVKIIGATSHYVTEELDEGPIIEQDIARV
SHIHSVEDFIMKGRDLERIVLARAIKLHSERKTMVYSNKTVVFS
>Mature_284_residues
MQKITILIHCKDQKGIIAAVTTFIARVEGNIMYIDQHVDVEQNVFFMRLECEFANHKITIESLKEEFDKTLAADFNMSWD
LYNQEQKPKMALFVSKYDHCLFDILGRYSAGELNVEIPVIISNHNDLRSIAERFDIPFHCVPFTKDNKEEGEAKQIELLK
RYEINFIVLARYMQIITPKLIELYENRIINIHHSFLPAFPGAKPYHSAFKRGVKIIGATSHYVTEELDEGPIIEQDIARV
SHIHSVEDFIMKGRDLERIVLARAIKLHSERKTMVYSNKTVVFS

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=187, Percent_Identity=25.1336898395722, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI209869995, Length=187, Percent_Identity=25.1336898395722, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI209869993, Length=187, Percent_Identity=25.1336898395722, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1787483, Length=288, Percent_Identity=41.3194444444444, Blast_Score=229, Evalue=1e-61,
Organism=Escherichia coli, GI1788846, Length=135, Percent_Identity=28.8888888888889, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17567511, Length=166, Percent_Identity=31.9277108433735, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33003; Mature: 33003

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKITILIHCKDQKGIIAAVTTFIARVEGNIMYIDQHVDVEQNVFFMRLECEFANHKITI
CCEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEEECCCEEEH
ESLKEEFDKTLAADFNMSWDLYNQEQKPKMALFVSKYDHCLFDILGRYSAGELNVEIPVI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEEEEEE
ISNHNDLRSIAERFDIPFHCVPFTKDNKEEGEAKQIELLKRYEINFIVLARYMQIITPKL
ECCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IELYENRIINIHHSFLPAFPGAKPYHSAFKRGVKIIGATSHYVTEELDEGPIIEQDIARV
HHHHHCCEEEEEHHHCCCCCCCCHHHHHHHCCEEEEECCHHHHHHHCCCCCCCHHHHHHH
SHIHSVEDFIMKGRDLERIVLARAIKLHSERKTMVYSNKTVVFS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCEEEEC
>Mature Secondary Structure
MQKITILIHCKDQKGIIAAVTTFIARVEGNIMYIDQHVDVEQNVFFMRLECEFANHKITI
CCEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEEECCCEEEH
ESLKEEFDKTLAADFNMSWDLYNQEQKPKMALFVSKYDHCLFDILGRYSAGELNVEIPVI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEEEEEE
ISNHNDLRSIAERFDIPFHCVPFTKDNKEEGEAKQIELLKRYEINFIVLARYMQIITPKL
ECCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IELYENRIINIHHSFLPAFPGAKPYHSAFKRGVKIIGATSHYVTEELDEGPIIEQDIARV
HHHHHCCEEEEEHHHCCCCCCCCHHHHHHHCCEEEEECCHHHHHHHCCCCCCCHHHHHHH
SHIHSVEDFIMKGRDLERIVLARAIKLHSERKTMVYSNKTVVFS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]