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The map label for this gene is queF

Identifier: 145300078

GI number: 145300078

Start: 3439311

End: 3440159

Strand: Direct

Name: queF

Synonym: ASA_3178

Alternate gene names: 145300078

Gene position: 3439311-3440159 (Clockwise)

Preceding gene: 145300076

Following gene: 145300079

Centisome position: 73.14

GC content: 59.95

Gene sequence:

>849_bases
ATGAGCAAACAAGAGAAATACGCCGGTGCCAGCGCCTTGCAAGGGCTGAGCCTGGGTCAGCAATCTGCCTACATCAGTCA
GTACAGCCCTGCCCTGCTGCAACCTGTGCCGCGCAGCCTCAATCGCGACGACCTCGGCCTGACCGGCGAGTTGCCTTTCC
AGGGTTGCGACGTCTGGACTCTGTACGAGCTCTCCTGGCTCAACGCCAGGGGTAAACCCATGGTGGCTGTGGGTGAGGTG
TCCGTGCCCGCAGTGAGCGCCAACCTCATCGAATCCAAGTCGTTCAAGCTCTATCTCAACTCCTTCAACCAGACCCGTTG
CGACTCGCTGGAGGCGGTACAAGCCATGCTGGTCAAGGATCTGAGTGCCTGCGCCGGCAGCGAAGTGAGCGTGACGCTGT
TCCCCCTGGCACAAGCTCCGCACCACATTGCCGCCCTGCCGGGTGAGTGCATCGATGAGCAGGATATAGAGGTTGATTGC
TATGAATTCGATGCCAATCTGCTGCAAGGGGCCGCGGGCAACGACGAGGTGGAAGAGACGCTGCACAGTCACCTGCTCAA
GTCCAACTGCCTGGTCACCAGCCAGCCCGACTGGGGCTCAGTGGTGATCCACTATCGCGGCCCACGGCTGGATCGGGAAA
AACTGCTGCGCTACCTCATCTCGTTTCGCCAGCACAACGAATTTCATGAACAGTGCATCGAGCGCATCTTTACCGATCTC
AAGCTCCTCTGCCACCCGAGCCAACTGACAGTGTATGCCCGCTACACTCGCCGTGGCGGGCTGGATATCAACCCGTTTCG
CAGCGACTGGGAGCTTCCCCCCACCAACCTGCGGCTGATCCGGCAGTAA

Upstream 100 bases:

>100_bases
CGTCACCATCCGGTGGCGCTCCACTGCAATATCCAGTAGGGGTAAGCGCCCCTGACGCTGCAGCGCAATGGCCCATTCAA
CATCCGGATCCATAAACACA

Downstream 100 bases:

>100_bases
AGTTGGGGTCCTGGCTGACAGAGAAGGCCCCTGGATATCGTATCTGTCAGTAAACTCTGGACGTGCAGGGGGGGGCTTGG
ATAACATGCCCACACCCCTG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSKQEKYAGASALQGLSLGQQSAYISQYSPALLQPVPRSLNRDDLGLTGELPFQGCDVWTLYELSWLNARGKPMVAVGEV
SVPAVSANLIESKSFKLYLNSFNQTRCDSLEAVQAMLVKDLSACAGSEVSVTLFPLAQAPHHIAALPGECIDEQDIEVDC
YEFDANLLQGAAGNDEVEETLHSHLLKSNCLVTSQPDWGSVVIHYRGPRLDREKLLRYLISFRQHNEFHEQCIERIFTDL
KLLCHPSQLTVYARYTRRGGLDINPFRSDWELPPTNLRLIRQ

Sequences:

>Translated_282_residues
MSKQEKYAGASALQGLSLGQQSAYISQYSPALLQPVPRSLNRDDLGLTGELPFQGCDVWTLYELSWLNARGKPMVAVGEV
SVPAVSANLIESKSFKLYLNSFNQTRCDSLEAVQAMLVKDLSACAGSEVSVTLFPLAQAPHHIAALPGECIDEQDIEVDC
YEFDANLLQGAAGNDEVEETLHSHLLKSNCLVTSQPDWGSVVIHYRGPRLDREKLLRYLISFRQHNEFHEQCIERIFTDL
KLLCHPSQLTVYARYTRRGGLDINPFRSDWELPPTNLRLIRQ
>Mature_281_residues
SKQEKYAGASALQGLSLGQQSAYISQYSPALLQPVPRSLNRDDLGLTGELPFQGCDVWTLYELSWLNARGKPMVAVGEVS
VPAVSANLIESKSFKLYLNSFNQTRCDSLEAVQAMLVKDLSACAGSEVSVTLFPLAQAPHHIAALPGECIDEQDIEVDCY
EFDANLLQGAAGNDEVEETLHSHLLKSNCLVTSQPDWGSVVIHYRGPRLDREKLLRYLISFRQHNEFHEQCIERIFTDLK
LLCHPSQLTVYARYTRRGGLDINPFRSDWELPPTNLRLIRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=279, Percent_Identity=60.573476702509, Blast_Score=342, Evalue=1e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_AERS4 (A4SQJ9)

Other databases:

- EMBL:   CP000644
- RefSeq:   YP_001142919.1
- ProteinModelPortal:   A4SQJ9
- SMR:   A4SQJ9
- STRING:   A4SQJ9
- GeneID:   4995889
- GenomeReviews:   CP000644_GR
- KEGG:   asa:ASA_3178
- eggNOG:   COG0780
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- PhylomeDB:   A4SQJ9
- ProtClustDB:   PRK11792
- BioCyc:   ASAL382245:ASA_3178-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 31607; Mature: 31476

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQEKYAGASALQGLSLGQQSAYISQYSPALLQPVPRSLNRDDLGLTGELPFQGCDVWT
CCCCHHHCCHHHHHHCCCCCHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEE
LYELSWLNARGKPMVAVGEVSVPAVSANLIESKSFKLYLNSFNQTRCDSLEAVQAMLVKD
EEEEEECCCCCCCEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
LSACAGSEVSVTLFPLAQAPHHIAALPGECIDEQDIEVDCYEFDANLLQGAAGNDEVEET
HHHHCCCCEEEEEEECCCCCCHHCCCCCCCCCCCCCEEEEEECCHHHHCCCCCCHHHHHH
LHSHLLKSNCLVTSQPDWGSVVIHYRGPRLDREKLLRYLISFRQHNEFHEQCIERIFTDL
HHHHHHHCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KLLCHPSQLTVYARYTRRGGLDINPFRSDWELPPTNLRLIRQ
HHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure 
SKQEKYAGASALQGLSLGQQSAYISQYSPALLQPVPRSLNRDDLGLTGELPFQGCDVWT
CCCHHHCCHHHHHHCCCCCHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEE
LYELSWLNARGKPMVAVGEVSVPAVSANLIESKSFKLYLNSFNQTRCDSLEAVQAMLVKD
EEEEEECCCCCCCEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
LSACAGSEVSVTLFPLAQAPHHIAALPGECIDEQDIEVDCYEFDANLLQGAAGNDEVEET
HHHHCCCCEEEEEEECCCCCCHHCCCCCCCCCCCCCEEEEEECCHHHHCCCCCCHHHHHH
LHSHLLKSNCLVTSQPDWGSVVIHYRGPRLDREKLLRYLISFRQHNEFHEQCIERIFTDL
HHHHHHHCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KLLCHPSQLTVYARYTRRGGLDINPFRSDWELPPTNLRLIRQ
HHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA