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The map label for this gene is purA [H]

Identifier: 145300021

GI number: 145300021

Start: 3370887

End: 3372143

Strand: Direct

Name: purA [H]

Synonym: ASA_3120

Alternate gene names: 145300021

Gene position: 3370887-3372143 (Clockwise)

Preceding gene: 145300019

Following gene: 145300022

Centisome position: 71.68

GC content: 61.65

Gene sequence:

>1257_bases
ATGTCGTCTATCGTAGTCGTAGGTGCTAACTGGGGTGATGAAGGCAAAGGCCGCATCGTCGATTATCTGGCAGGTCAATC
CGCCGCCAGCATCCGTTTTCAGGGCGGCAACAACGCCGGCCACACCGTCGTCAACGACCTGGGCACCTTCAAGCTGCACC
AGTTGCCGAGCGGCGTATTCAACCCGGAGTGCCTAGTTGTCCTCGGCCCTGGCATGGTCATCAGCCCGGAGCCATTGACC
CGCGAGCTGGCTGAAGTGAAAGCCGCCGGCGTGACCCCTAAACTAGCCATCTCTGATCGCGCTACCTTCTGCCTGCCGAT
CCATGCGCTGGAAGATACCCTGGAAGAAGTCCGTCTGGGTGATGGCGCCTATGGCTCCACCCGTCAGGGCATTGCACCGG
CTTACGGTGATCGGGTCATGAAAAAAGCGATCCTGGTCGGCTGGCTGAAACAGCCTGAAGTGCTGGTCGAGCGCATCCAG
TTCTTCCTCGACTGGAAACTGCCGCAGCTCAAAGCCCTCTACCCGAGCTTCGAGTTCAGCCAGACCGCCCAGGAAATGGC
CGACTGGCTGCTGGAAGTGTCCGCTCCCTGGATCGACGCCGTCTGCAACGTCAGCGAGCCGCTCAAGGCCATCCAGGCCA
AGAATGGCACCCTGCTGTTCGAAGCCCAGCTGGGCGCCGGCCGTGATCTCATCTACGGCGAATACCCCTGGGTGACCTCT
TCCCACGTTTCCGGCGCTTACGCCGGCATCGGTGGCGGCCTGCCAGCCCTGCGCCCCGAGCGCGTGATTGCCGTGGCCAA
GGCGTTCAGTACCTCTGTTGGTACCGGCACCCTGATCACCGCCATGGAGAACCAGGAAGAGTTCCGCAAGTTCGCCAACG
AGTTCGGTGCCACCACTGGCCGTCCCCGTGACATCGGCTACTTCGACGCCGTTGCTACCAAGCAGGGTCTGGAGATCCAG
GCCGCCAACGAGATCGCCCTGACCAAGCTGGACTGCCTCTCCGGCATGCCGGATCTGAAGATCTGTGTGGCCTACGAAGG
TGCCAACACCGAGAACCCGATCTGGCCACAGACCGCCGGTCTGAAGCCGGTGTACGAGTCCATGGAAGCCTGGAGCGAAG
ACATCTCCCACATCCGCAAGTTTGAAGACTTGCCCAAGGCTGCCCAGCACTATGTGCTGCGTATCGAAGCCCTGCTGGGT
GTGCCGGTTCCCATGGTCTCCGTCGGCCCGGGTCGCGAGCAGATGATACTGCGCTAA

Upstream 100 bases:

>100_bases
TAATTATATGGTTCTCTTATCGGAATTATAAAGATAATTAATTTTTCTAATCTGCCTTAGCCTCCTAGGATGAACGCCTA
ACGTCTTAGGAGAAAGAGTC

Downstream 100 bases:

>100_bases
GCCGCCTGCTCTTCTGTCAGCACAATGTCAAAACCGGCCTCCATGAGGCCGGTTTTTATGGCCGCCACACCACGAGCTGG
CCCACTCCCTTGCCACCTCG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase 2; AdSS 2; IMP--aspartate ligase 2 [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MSSIVVVGANWGDEGKGRIVDYLAGQSAASIRFQGGNNAGHTVVNDLGTFKLHQLPSGVFNPECLVVLGPGMVISPEPLT
RELAEVKAAGVTPKLAISDRATFCLPIHALEDTLEEVRLGDGAYGSTRQGIAPAYGDRVMKKAILVGWLKQPEVLVERIQ
FFLDWKLPQLKALYPSFEFSQTAQEMADWLLEVSAPWIDAVCNVSEPLKAIQAKNGTLLFEAQLGAGRDLIYGEYPWVTS
SHVSGAYAGIGGGLPALRPERVIAVAKAFSTSVGTGTLITAMENQEEFRKFANEFGATTGRPRDIGYFDAVATKQGLEIQ
AANEIALTKLDCLSGMPDLKICVAYEGANTENPIWPQTAGLKPVYESMEAWSEDISHIRKFEDLPKAAQHYVLRIEALLG
VPVPMVSVGPGREQMILR

Sequences:

>Translated_418_residues
MSSIVVVGANWGDEGKGRIVDYLAGQSAASIRFQGGNNAGHTVVNDLGTFKLHQLPSGVFNPECLVVLGPGMVISPEPLT
RELAEVKAAGVTPKLAISDRATFCLPIHALEDTLEEVRLGDGAYGSTRQGIAPAYGDRVMKKAILVGWLKQPEVLVERIQ
FFLDWKLPQLKALYPSFEFSQTAQEMADWLLEVSAPWIDAVCNVSEPLKAIQAKNGTLLFEAQLGAGRDLIYGEYPWVTS
SHVSGAYAGIGGGLPALRPERVIAVAKAFSTSVGTGTLITAMENQEEFRKFANEFGATTGRPRDIGYFDAVATKQGLEIQ
AANEIALTKLDCLSGMPDLKICVAYEGANTENPIWPQTAGLKPVYESMEAWSEDISHIRKFEDLPKAAQHYVLRIEALLG
VPVPMVSVGPGREQMILR
>Mature_417_residues
SSIVVVGANWGDEGKGRIVDYLAGQSAASIRFQGGNNAGHTVVNDLGTFKLHQLPSGVFNPECLVVLGPGMVISPEPLTR
ELAEVKAAGVTPKLAISDRATFCLPIHALEDTLEEVRLGDGAYGSTRQGIAPAYGDRVMKKAILVGWLKQPEVLVERIQF
FLDWKLPQLKALYPSFEFSQTAQEMADWLLEVSAPWIDAVCNVSEPLKAIQAKNGTLLFEAQLGAGRDLIYGEYPWVTSS
HVSGAYAGIGGGLPALRPERVIAVAKAFSTSVGTGTLITAMENQEEFRKFANEFGATTGRPRDIGYFDAVATKQGLEIQA
ANEIALTKLDCLSGMPDLKICVAYEGANTENPIWPQTAGLKPVYESMEAWSEDISHIRKFEDLPKAAQHYVLRIEALLGV
PVPMVSVGPGREQMILR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=432, Percent_Identity=34.2592592592593, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI22748717, Length=428, Percent_Identity=32.9439252336449, Blast_Score=198, Evalue=8e-51,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=31.077694235589, Blast_Score=164, Evalue=1e-40,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=36.0655737704918, Blast_Score=265, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI25146553, Length=439, Percent_Identity=33.7129840546697, Blast_Score=214, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI25146555, Length=439, Percent_Identity=33.7129840546697, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6324109, Length=440, Percent_Identity=34.5454545454545, Blast_Score=223, Evalue=6e-59,
Organism=Drosophila melanogaster, GI21356033, Length=426, Percent_Identity=35.9154929577465, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 45256; Mature: 45125

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIVVVGANWGDEGKGRIVDYLAGQSAASIRFQGGNNAGHTVVNDLGTFKLHQLPSGVF
CCCEEEEECCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCHHHHHCCCEEHHHCCCCCC
NPECLVVLGPGMVISPEPLTRELAEVKAAGVTPKLAISDRATFCLPIHALEDTLEEVRLG
CCCEEEEECCCEEECCCHHHHHHHHHHHCCCCCEEEECCCCEEEEEHHHHHHHHHHHHCC
DGAYGSTRQGIAPAYGDRVMKKAILVGWLKQPEVLVERIQFFLDWKLPQLKALYPSFEFS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHH
QTAQEMADWLLEVSAPWIDAVCNVSEPLKAIQAKNGTLLFEAQLGAGRDLIYGEYPWVTS
HHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCCCCC
SHVSGAYAGIGGGLPALRPERVIAVAKAFSTSVGTGTLITAMENQEEFRKFANEFGATTG
CCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCC
RPRDIGYFDAVATKQGLEIQAANEIALTKLDCLSGMPDLKICVAYEGANTENPIWPQTAG
CCCCCCHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCC
LKPVYESMEAWSEDISHIRKFEDLPKAAQHYVLRIEALLGVPVPMVSVGPGREQMILR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure 
SSIVVVGANWGDEGKGRIVDYLAGQSAASIRFQGGNNAGHTVVNDLGTFKLHQLPSGVF
CCEEEEECCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCHHHHHCCCEEHHHCCCCCC
NPECLVVLGPGMVISPEPLTRELAEVKAAGVTPKLAISDRATFCLPIHALEDTLEEVRLG
CCCEEEEECCCEEECCCHHHHHHHHHHHCCCCCEEEECCCCEEEEEHHHHHHHHHHHHCC
DGAYGSTRQGIAPAYGDRVMKKAILVGWLKQPEVLVERIQFFLDWKLPQLKALYPSFEFS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHH
QTAQEMADWLLEVSAPWIDAVCNVSEPLKAIQAKNGTLLFEAQLGAGRDLIYGEYPWVTS
HHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCCCCC
SHVSGAYAGIGGGLPALRPERVIAVAKAFSTSVGTGTLITAMENQEEFRKFANEFGATTG
CCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCC
RPRDIGYFDAVATKQGLEIQAANEIALTKLDCLSGMPDLKICVAYEGANTENPIWPQTAG
CCCCCCHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCC
LKPVYESMEAWSEDISHIRKFEDLPKAAQHYVLRIEALLGVPVPMVSVGPGREQMILR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA