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The map label for this gene is appA [H]

Identifier: 145300001

GI number: 145300001

Start: 3344816

End: 3346501

Strand: Direct

Name: appA [H]

Synonym: ASA_3100

Alternate gene names: 145300001

Gene position: 3344816-3346501 (Clockwise)

Preceding gene: 145300000

Following gene: 145300002

Centisome position: 71.13

GC content: 57.0

Gene sequence:

>1686_bases
ATGCTTGCCAAAGTATCCAAAATCTCTATTGCTGTAATGCTGGGAACCATGATGCTTGCCGGGGCCGCCGGCAATGTCAT
GGCTGCTGAAAACAAGACTGTGCTGACCCTGGTCGCACAACAGGAGACCGCCTGGGTCAAAAACTTCAACCCCTTCCTGC
AAGCGGGTTTGCTCCATACCACTCGTCACTTCATCTATGAACCTCTGGTGATCTTCAACGATATGCAGGGTGGCAAACCC
GTCTATCGTCTGGCCACTCACTATGCGTTCAGCGATGACCTGAAATCCGTCACCTTCGATCTGCGTGAAGGGGTCAAGTG
GTCCGACGGGGAAGGTTTTGATGCGGATGATATCCTCTTCTCCTTCAATCTGGTTAAAGCCAACAAGGCGCTGGATGAGC
GCTCTATCTGGACCCAGATCAAGAACATAGAGAAATTAGGTCCCTACAAGATCAAGTTCGACCTGACCGACGTCAATACC
AACGTGGTGAATGACCTGGTGCTGGTGCCCATAGTCCCCGAGCACCAGTGGAAGTCAGTGAAGGATCCGGTTGCCTTTAC
CAACGACAAGCCGGTCGGTACCGGTCCCTTCACCGAGGTGGACAACTTCTCCGCCCAGCTCTACACCCAGTGTCGCAACC
CGCACTACTGGGACAACGCCAACCTCGCCATCGACTGCCTGCGCATGCCGCAGATGGCCACCAACGACCAGGTGTTGGCC
GCTGTCATGAAGGGTGACGTTGACTGGTTCGGCTCCTTCGTACCGGACATAGAGCGCCTCTACGTTGGCAACGATCCGAA
AAACAACAAGTACTGGTTCCCGGCCTCCGGCACAGTGGCATTCAACGTCAACTTCCAGACCAAGAACCCGGGCAACCACG
AAGCCTTCAACGACATCACTTTCCGCCGCGCCTTCTCCATGGCGATGGACCGTCAGGCCATGGTGGATATTGCCGGTTAC
GGCTACCCGACCGTCAACGAGTACCCATCAGGGCTCGGCAAAGCCTTCGACTCCTGGAACAACCCGGAAGTCGACAAGAA
GTACGGCAAATTCAACAAGTTCGACTTGGAAGGCGCCAAGGCCATGCTGAAAGAGGCGGGCTACAAGGATTGTGACGGCG
ACAAGTTCCTCGACACCCCGAGCTGCAAGAAGATCGAGTTCAAGGTGCTGGTACCGAACGGCTGGACCGACTGGGTCAAT
ACCGCCCAGATCGGGGTCGAAGGCTTGCAGGCCCTGGGCCTGAACGCCAAGACCGCCACCCCGGAAGCCACCGTCTGGAC
CGAAAACCTGATCACCGGTGACTTCGACGTCGCGCTGCAGGGTTACTTTGCCGGTGCCAACCCGCACAAGTACTTCGAGA
CAGCCTTCCACTCCCGCAACATGGGTGAGCGTGGCAACCGCTTTGCGGCGCCCCGTTACAAGGACCCGGCCCTCGACCAG
CTGATCGATGACTTCACCCAGACCGCCGATGCGGCCAAGCAGAAGGCCATCATGTTCGAGATCCAGGACAAGGTCGGTGC
CAACCAGACCATCATCCCTGTGTTCAACAACCCGACCTGGTACGAATACAGCACCAAGCGCTTCAATGGCTGGTTCAGTG
CCGACAACCCGGTTGCCAAGCCGCAGGTTCACCCGGATACCCCGGAACGTCTGCTGCACGTGTTGTCACTCAAGCCAAAC
AGCTAA

Upstream 100 bases:

>100_bases
TGACCAACACAAAGGGCCGACCTCCCCAGCATGCGAAGTCGGTGCTCACACAAAACAAGGCGAACCCTCGGGAATGCCAA
AAAATAAGAAAGGACATAAT

Downstream 100 bases:

>100_bases
CCACCCCGGCCCCCTGCGGGGGCCATTCTCCCCGCCATGTCTTCCATCCCCCTGATTGTTCGGGGTTGGGGGAGCTTTGC
CCGGCGCGGGGCAGGCTTGC

Product: ABC-type oligopeptide transporter, periplasmic binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MLAKVSKISIAVMLGTMMLAGAAGNVMAAENKTVLTLVAQQETAWVKNFNPFLQAGLLHTTRHFIYEPLVIFNDMQGGKP
VYRLATHYAFSDDLKSVTFDLREGVKWSDGEGFDADDILFSFNLVKANKALDERSIWTQIKNIEKLGPYKIKFDLTDVNT
NVVNDLVLVPIVPEHQWKSVKDPVAFTNDKPVGTGPFTEVDNFSAQLYTQCRNPHYWDNANLAIDCLRMPQMATNDQVLA
AVMKGDVDWFGSFVPDIERLYVGNDPKNNKYWFPASGTVAFNVNFQTKNPGNHEAFNDITFRRAFSMAMDRQAMVDIAGY
GYPTVNEYPSGLGKAFDSWNNPEVDKKYGKFNKFDLEGAKAMLKEAGYKDCDGDKFLDTPSCKKIEFKVLVPNGWTDWVN
TAQIGVEGLQALGLNAKTATPEATVWTENLITGDFDVALQGYFAGANPHKYFETAFHSRNMGERGNRFAAPRYKDPALDQ
LIDDFTQTADAAKQKAIMFEIQDKVGANQTIIPVFNNPTWYEYSTKRFNGWFSADNPVAKPQVHPDTPERLLHVLSLKPN
S

Sequences:

>Translated_561_residues
MLAKVSKISIAVMLGTMMLAGAAGNVMAAENKTVLTLVAQQETAWVKNFNPFLQAGLLHTTRHFIYEPLVIFNDMQGGKP
VYRLATHYAFSDDLKSVTFDLREGVKWSDGEGFDADDILFSFNLVKANKALDERSIWTQIKNIEKLGPYKIKFDLTDVNT
NVVNDLVLVPIVPEHQWKSVKDPVAFTNDKPVGTGPFTEVDNFSAQLYTQCRNPHYWDNANLAIDCLRMPQMATNDQVLA
AVMKGDVDWFGSFVPDIERLYVGNDPKNNKYWFPASGTVAFNVNFQTKNPGNHEAFNDITFRRAFSMAMDRQAMVDIAGY
GYPTVNEYPSGLGKAFDSWNNPEVDKKYGKFNKFDLEGAKAMLKEAGYKDCDGDKFLDTPSCKKIEFKVLVPNGWTDWVN
TAQIGVEGLQALGLNAKTATPEATVWTENLITGDFDVALQGYFAGANPHKYFETAFHSRNMGERGNRFAAPRYKDPALDQ
LIDDFTQTADAAKQKAIMFEIQDKVGANQTIIPVFNNPTWYEYSTKRFNGWFSADNPVAKPQVHPDTPERLLHVLSLKPN
S
>Mature_561_residues
MLAKVSKISIAVMLGTMMLAGAAGNVMAAENKTVLTLVAQQETAWVKNFNPFLQAGLLHTTRHFIYEPLVIFNDMQGGKP
VYRLATHYAFSDDLKSVTFDLREGVKWSDGEGFDADDILFSFNLVKANKALDERSIWTQIKNIEKLGPYKIKFDLTDVNT
NVVNDLVLVPIVPEHQWKSVKDPVAFTNDKPVGTGPFTEVDNFSAQLYTQCRNPHYWDNANLAIDCLRMPQMATNDQVLA
AVMKGDVDWFGSFVPDIERLYVGNDPKNNKYWFPASGTVAFNVNFQTKNPGNHEAFNDITFRRAFSMAMDRQAMVDIAGY
GYPTVNEYPSGLGKAFDSWNNPEVDKKYGKFNKFDLEGAKAMLKEAGYKDCDGDKFLDTPSCKKIEFKVLVPNGWTDWVN
TAQIGVEGLQALGLNAKTATPEATVWTENLITGDFDVALQGYFAGANPHKYFETAFHSRNMGERGNRFAAPRYKDPALDQ
LIDDFTQTADAAKQKAIMFEIQDKVGANQTIIPVFNNPTWYEYSTKRFNGWFSADNPVAKPQVHPDTPERLLHVLSLKPN
S

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=494, Percent_Identity=22.4696356275304, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62932; Mature: 62932

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKVSKISIAVMLGTMMLAGAAGNVMAAENKTVLTLVAQQETAWVKNFNPFLQAGLLHT
CCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCHHHHHCCCHHHHHHHHHH
TRHFIYEPLVIFNDMQGGKPVYRLATHYAFSDDLKSVTFDLREGVKWSDGEGFDADDILF
HHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
SFNLVKANKALDERSIWTQIKNIEKLGPYKIKFDLTDVNTNVVNDLVLVPIVPEHQWKSV
EEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHCCEEEEEECCCCCCCCC
KDPVAFTNDKPVGTGPFTEVDNFSAQLYTQCRNPHYWDNANLAIDCLRMPQMATNDQVLA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHH
AVMKGDVDWFGSFVPDIERLYVGNDPKNNKYWFPASGTVAFNVNFQTKNPGNHEAFNDIT
HHHHCCHHHHHHHCCCCCEEEECCCCCCCCEEECCCCEEEEEEEEEECCCCCCCHHHHHH
FRRAFSMAMDRQAMVDIAGYGYPTVNEYPSGLGKAFDSWNNPEVDKKYGKFNKFDLEGAK
HHHHHHHHHCCHHHEEEECCCCCCHHHCCCHHHHHHHCCCCCCCHHHHCCCCCCCCHHHH
AMLKEAGYKDCDGDKFLDTPSCKKIEFKVLVPNGWTDWVNTAQIGVEGLQALGLNAKTAT
HHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCC
PEATVWTENLITGDFDVALQGYFAGANPHKYFETAFHSRNMGERGNRFAAPRYKDPALDQ
CCCEEEECCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHH
LIDDFTQTADAAKQKAIMFEIQDKVGANQTIIPVFNNPTWYEYSTKRFNGWFSADNPVAK
HHHHHHHHHHHHHHCEEEEEEHHHCCCCCEEEEEECCCCEEEECCCEECCEECCCCCCCC
PQVHPDTPERLLHVLSLKPNS
CCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MLAKVSKISIAVMLGTMMLAGAAGNVMAAENKTVLTLVAQQETAWVKNFNPFLQAGLLHT
CCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCHHHHHCCCHHHHHHHHHH
TRHFIYEPLVIFNDMQGGKPVYRLATHYAFSDDLKSVTFDLREGVKWSDGEGFDADDILF
HHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
SFNLVKANKALDERSIWTQIKNIEKLGPYKIKFDLTDVNTNVVNDLVLVPIVPEHQWKSV
EEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCHHHCCEEEEEECCCCCCCCC
KDPVAFTNDKPVGTGPFTEVDNFSAQLYTQCRNPHYWDNANLAIDCLRMPQMATNDQVLA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHH
AVMKGDVDWFGSFVPDIERLYVGNDPKNNKYWFPASGTVAFNVNFQTKNPGNHEAFNDIT
HHHHCCHHHHHHHCCCCCEEEECCCCCCCCEEECCCCEEEEEEEEEECCCCCCCHHHHHH
FRRAFSMAMDRQAMVDIAGYGYPTVNEYPSGLGKAFDSWNNPEVDKKYGKFNKFDLEGAK
HHHHHHHHHCCHHHEEEECCCCCCHHHCCCHHHHHHHCCCCCCCHHHHCCCCCCCCHHHH
AMLKEAGYKDCDGDKFLDTPSCKKIEFKVLVPNGWTDWVNTAQIGVEGLQALGLNAKTAT
HHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCC
PEATVWTENLITGDFDVALQGYFAGANPHKYFETAFHSRNMGERGNRFAAPRYKDPALDQ
CCCEEEECCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHH
LIDDFTQTADAAKQKAIMFEIQDKVGANQTIIPVFNNPTWYEYSTKRFNGWFSADNPVAK
HHHHHHHHHHHHHHCEEEEEEHHHCCCCCEEEEEECCCCEEEECCCEECCEECCCCCCCC
PQVHPDTPERLLHVLSLKPNS
CCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]