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The map label for this gene is puuP [H]

Identifier: 145299930

GI number: 145299930

Start: 3256186

End: 3257511

Strand: Direct

Name: puuP [H]

Synonym: ASA_3022

Alternate gene names: 145299930

Gene position: 3256186-3257511 (Clockwise)

Preceding gene: 145299929

Following gene: 145299931

Centisome position: 69.25

GC content: 59.73

Gene sequence:

>1326_bases
ATGGCTCAACGCCCGGCTCGATTGAAGCAAACCCTGACCCTGTGGCAAGTGGTGGTGATGGGATTGGCCTATATGACCCC
CATGGTGGTGTTCGATACCTTTGGCATCGTCACCGACATCACTCAGGGCCATGTGACTACCGCCTACCTGCTGGCCCTGG
TGGGCGTGCTGTTCACCGCCACCAGCTATAGCCAGATGGTGCGCCACTATCCGGTTGCCGGCTCCGCCTATACCTATGCC
CAGAAGGAGTTCTCCCCCGTGGTGGGCTTCATGGTGGGCTGGTCATCCCTGCTCGACTACATGTTCCTGCCGATGATCAA
CATGCTGCTGGCCAAGATCTATCTGACCGCCCTGTTTCCCGGCATCGAACCCTGGATCTTCATCTGCGCCATGACGGCAC
TGATGACCCTCATCAACCTGCGCGGCATCGATCTGGTGGCCAACCTCAACGGCGTCATCATCTTGATCCAGATCGCCATC
ATGGTGGTGTTTCTGGCCCTGATCGGATACGGCATCGCTCAGGGTGAAGGGCTCGGCACCGTCAGCCTGCAGCCCATCTA
CACCGCCGACACAGAAGTGTTGCCACTGCTGACCGGCGCCACCATCCTCTGTTTCTCCTTTCTGGGATTTGACAGCCTGA
GCGCCCTCAGTGAAGAGACCCCCAACGCCGGCAAGGTGATCCCCAAGGCCATCTTCCTGACGGCCTTGCTGGGCGGCCTC
ATCTTCGTGGTGGTGGCCTATGCCCTGCAGCTCTACTTCCCGGATGCCTCCCGCTTCAAGGAGCCGGATGCCGTGCTGCC
CGAGATTGCCCTCTATGTGGGCGGCAAGCTGTTCCAGAGCGTGGTGCTGGTCTGTGCCTGCGTGGCCGTGCTGGCGTCGG
GACTCTCCTCCCACGCTGGTGTCTCCCGCATCCTCTACGTGATGGGGCGGGACAAGGTGCTGCCCCAGCGCACCTTCGGC
TATATCCACCCCAAGTGGCAGACACCCGTGTTCAATATCCTGCTGGTGGGCATACTGGCGCTGTCGGCCATCAGCTTCGA
TCTGGAAATCGCCCTGGCCCTGATCAACTTCGGTGCCCTGGTGGCCTTTACCGTGGTCAATTTGTCGGTGATCGCCTGCT
ATTACGTGCGTCAGGGGCGCAACAAGACCCTCAGGGATCACCTGCAATATCTGGTGCTGCCCCTGTGCGGGGCGGGGACT
GTCGCGATTCTCTGGCTCAATCTCGAACTAACCTCTCTTATCCTCGGCATTATCTGGGCTGCCATAGGGGGAATTTATCT
GTTTTTGCGTACCACCTTCTGGCGCGTATCCTTGCCGACCTATTGA

Upstream 100 bases:

>100_bases
ACAGGCGCGCTGGGCTCCCTCCAAGGCCAGCCACACTGTCACGCGTCCTTCGCCTGCCTGCGGCGCGGGACATACGCCCC
AAATTCGAGGAGAGAGGCCA

Downstream 100 bases:

>100_bases
GCTGAAAGCCTTGCCAACACTGGGCTGGCGCCGATCTCCCCCGAACGGGTGACGGCGCCATTTATTGATATAAAACAGGT
CATTGATAATTCTCTTGCCC

Product: amino acid permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MAQRPARLKQTLTLWQVVVMGLAYMTPMVVFDTFGIVTDITQGHVTTAYLLALVGVLFTATSYSQMVRHYPVAGSAYTYA
QKEFSPVVGFMVGWSSLLDYMFLPMINMLLAKIYLTALFPGIEPWIFICAMTALMTLINLRGIDLVANLNGVIILIQIAI
MVVFLALIGYGIAQGEGLGTVSLQPIYTADTEVLPLLTGATILCFSFLGFDSLSALSEETPNAGKVIPKAIFLTALLGGL
IFVVVAYALQLYFPDASRFKEPDAVLPEIALYVGGKLFQSVVLVCACVAVLASGLSSHAGVSRILYVMGRDKVLPQRTFG
YIHPKWQTPVFNILLVGILALSAISFDLEIALALINFGALVAFTVVNLSVIACYYVRQGRNKTLRDHLQYLVLPLCGAGT
VAILWLNLELTSLILGIIWAAIGGIYLFLRTTFWRVSLPTY

Sequences:

>Translated_441_residues
MAQRPARLKQTLTLWQVVVMGLAYMTPMVVFDTFGIVTDITQGHVTTAYLLALVGVLFTATSYSQMVRHYPVAGSAYTYA
QKEFSPVVGFMVGWSSLLDYMFLPMINMLLAKIYLTALFPGIEPWIFICAMTALMTLINLRGIDLVANLNGVIILIQIAI
MVVFLALIGYGIAQGEGLGTVSLQPIYTADTEVLPLLTGATILCFSFLGFDSLSALSEETPNAGKVIPKAIFLTALLGGL
IFVVVAYALQLYFPDASRFKEPDAVLPEIALYVGGKLFQSVVLVCACVAVLASGLSSHAGVSRILYVMGRDKVLPQRTFG
YIHPKWQTPVFNILLVGILALSAISFDLEIALALINFGALVAFTVVNLSVIACYYVRQGRNKTLRDHLQYLVLPLCGAGT
VAILWLNLELTSLILGIIWAAIGGIYLFLRTTFWRVSLPTY
>Mature_440_residues
AQRPARLKQTLTLWQVVVMGLAYMTPMVVFDTFGIVTDITQGHVTTAYLLALVGVLFTATSYSQMVRHYPVAGSAYTYAQ
KEFSPVVGFMVGWSSLLDYMFLPMINMLLAKIYLTALFPGIEPWIFICAMTALMTLINLRGIDLVANLNGVIILIQIAIM
VVFLALIGYGIAQGEGLGTVSLQPIYTADTEVLPLLTGATILCFSFLGFDSLSALSEETPNAGKVIPKAIFLTALLGGLI
FVVVAYALQLYFPDASRFKEPDAVLPEIALYVGGKLFQSVVLVCACVAVLASGLSSHAGVSRILYVMGRDKVLPQRTFGY
IHPKWQTPVFNILLVGILALSAISFDLEIALALINFGALVAFTVVNLSVIACYYVRQGRNKTLRDHLQYLVLPLCGAGTV
AILWLNLELTSLILGIIWAAIGGIYLFLRTTFWRVSLPTY

Specific function: Imports putrescine [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Homo sapiens, GI4507047, Length=421, Percent_Identity=23.2779097387173, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI114326544, Length=223, Percent_Identity=29.5964125560538, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI114326550, Length=223, Percent_Identity=29.5964125560538, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI87081869, Length=431, Percent_Identity=62.877030162413, Blast_Score=573, Evalue=1e-164,
Organism=Escherichia coli, GI87082023, Length=436, Percent_Identity=61.4678899082569, Blast_Score=546, Evalue=1e-157,
Organism=Escherichia coli, GI87082250, Length=350, Percent_Identity=25.4285714285714, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786789, Length=174, Percent_Identity=25.8620689655172, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788480, Length=363, Percent_Identity=23.1404958677686, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17532491, Length=425, Percent_Identity=23.2941176470588, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17533459, Length=398, Percent_Identity=22.8643216080402, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17531343, Length=398, Percent_Identity=22.8643216080402, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24668806, Length=410, Percent_Identity=25.1219512195122, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356285, Length=410, Percent_Identity=25.1219512195122, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24668802, Length=410, Percent_Identity=25.1219512195122, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24667468, Length=405, Percent_Identity=25.1851851851852, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24666159, Length=407, Percent_Identity=22.3587223587224, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI221512776, Length=407, Percent_Identity=22.3587223587224, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281366235, Length=182, Percent_Identity=32.4175824175824, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI116007820, Length=182, Percent_Identity=32.4175824175824, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 48094; Mature: 47963

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQRPARLKQTLTLWQVVVMGLAYMTPMVVFDTFGIVTDITQGHVTTAYLLALVGVLFTA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
TSYSQMVRHYPVAGSAYTYAQKEFSPVVGFMVGWSSLLDYMFLPMINMLLAKIYLTALFP
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GIEPWIFICAMTALMTLINLRGIDLVANLNGVIILIQIAIMVVFLALIGYGIAQGEGLGT
CCHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
VSLQPIYTADTEVLPLLTGATILCFSFLGFDSLSALSEETPNAGKVIPKAIFLTALLGGL
EEECEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
IFVVVAYALQLYFPDASRFKEPDAVLPEIALYVGGKLFQSVVLVCACVAVLASGLSSHAG
HHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VSRILYVMGRDKVLPQRTFGYIHPKWQTPVFNILLVGILALSAISFDLEIALALINFGAL
HHHHHHHHCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAFTVVNLSVIACYYVRQGRNKTLRDHLQYLVLPLCGAGTVAILWLNLELTSLILGIIWA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHH
AIGGIYLFLRTTFWRVSLPTY
HHHHHHHHHHHHHHEEECCCC
>Mature Secondary Structure 
AQRPARLKQTLTLWQVVVMGLAYMTPMVVFDTFGIVTDITQGHVTTAYLLALVGVLFTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
TSYSQMVRHYPVAGSAYTYAQKEFSPVVGFMVGWSSLLDYMFLPMINMLLAKIYLTALFP
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GIEPWIFICAMTALMTLINLRGIDLVANLNGVIILIQIAIMVVFLALIGYGIAQGEGLGT
CCHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
VSLQPIYTADTEVLPLLTGATILCFSFLGFDSLSALSEETPNAGKVIPKAIFLTALLGGL
EEECEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
IFVVVAYALQLYFPDASRFKEPDAVLPEIALYVGGKLFQSVVLVCACVAVLASGLSSHAG
HHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VSRILYVMGRDKVLPQRTFGYIHPKWQTPVFNILLVGILALSAISFDLEIALALINFGAL
HHHHHHHHCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAFTVVNLSVIACYYVRQGRNKTLRDHLQYLVLPLCGAGTVAILWLNLELTSLILGIIWA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHH
AIGGIYLFLRTTFWRVSLPTY
HHHHHHHHHHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]