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The map label for this gene is cusB [H]

Identifier: 145299886

GI number: 145299886

Start: 3204596

End: 3206131

Strand: Direct

Name: cusB [H]

Synonym: ASA_2977

Alternate gene names: 145299886

Gene position: 3204596-3206131 (Clockwise)

Preceding gene: 145299885

Following gene: 145299887

Centisome position: 68.15

GC content: 62.43

Gene sequence:

>1536_bases
ATGAGCCATAAATCAAGCCAAAAGCTGCTTGTCTCAGCCCTGTTGCTGGCCCTCGGGGCCGGTGGCGGCTACTGGGCCGC
CAAACAGACTGGCGACGGTGCAACCGCCAAAGAGAAGACCCCCCTCTACTGGGTCAACCCCATGGATCCCCGCGACAAGC
GCGACGGCCCAGCCAAGGACAACATGGGGATGGACTTTATCCCCGTCTATGAGGAGCAAAAAAGCGGCTCGCCGGGTACC
GTCACCATCAGCCCCGAGATCCAGCAGAATCTGGGGGTGCGGCTAGCCAAAGTGGAGCGACTGCCCATCCACCAGCAGAT
CGAGACAGTGGGTTACGTCGGCTATGACGAAGATCGGCTGGAGGCAGTCAATGCCCGCATGGCGGGCTGGATCCGCACCC
TCGCCATCAAGAGCGAGGGGCAGAAGGTGGCCAAAGGCTCCCTGCTCTACGAGATCTACGCCCCGGATCTGGTCAACGCC
CAGCACGAGTACCTGCTAGCCCTCAACACGACCAACCCGCTGCTGCTGCTGCGCGCCGCCGAGGGCAAGCTCAAATCCCT
GCAGGTACCGGCGGATCAAATCGCCGCCCTCAAGCGCAACCGTCAGGTACGTGAAACCATTGGCATCTATGCCCCTAGCA
GCGGTTATGTCTCAGAGCTGAAAGTACGCGAGGGTCAGTATGTGGAGCCCGCCGCCGCCCTGTTCAATATCAGCACCCTG
CAGCAAGTGTGGGTGAGCGCCGAGGTGTTCGAGCGCCAGGCCGCACAGCTCAAAGTGGGGGATCCGGTCACCATGACCCT
CGACTACGCCCCCGGTCGCAGCTGGCAGGGTCGGGTGGATTACCTCTACCCGACGCTCGATGCCGCCACTCGCACCCTCA
AGGTGCGGCTGCGCGTTGACAATCCGGACGAGTTTTTGAAACCCAATATGTTCGCCAAGGTAGCCATTCGCACTGGCCAG
GGGGAACCCCGGCTGGTGGTACCGAGCGAAGCGGTGATCCGCACCGGCAGCCAGGACAGGCTGGTGCTGGCACTGGGGGA
TGGCAACTTCAAGTCGGTGGCCGTCACCCTGGGCTCCCAGTTTGGCGACAAGGTCGCCATCAAGGCAGGGGTAGAAACGG
GTGACAGCATAGTCAGCTCGGCCCAGTTCCTGCTCGACTCCGAATCGGCCATCGATTCGGACTTCCAGCGCATGACAGCG
GTGCGCCCGGCGCAGGTGTGGACTCAAGGGACTGTGCAAAGCATGGATCTGGCCAGCCGCACCCTGATGGTGGCCCACCC
GCCCATCCCCGAATGGCAGTGGCCCGCCATGGAGATGGAGTTCACCGTCGCCGAAGGAGTCGATATCAGCAAGCTGGCCG
AGGGGCAAACCCTCCATCTGCAGGTGATACAAGAGGGGGATGAGTACCGCATCACCACCATCCATCAGGAGAGTGCCAAG
GAAAGTGCCCCGGCCACCGGCCACGCTCCGGAGCAGGCAACAGATGAGATGGAAGGCATGGACCATAGCCAGCACGGTAT
GGGGGCCAAGCCATGA

Upstream 100 bases:

>100_bases
CAGAAGAGAAGATGCACATGTCATCTGCCCGATTGACCGAACCTGTTGCCGCTCACCCTTGCGCCATTAGCGCCCGCCTG
ATGCAGGTCGGAGGTTCCCT

Downstream 100 bases:

>100_bases
TCCCTGCCATTATTCGCTGGTCCGTGGGCAACCGCTTTCTGGTGCTGTTGTTGACCCTCATGCTCACCGCCTGGGGGCTC
TGGTCAGTGAAGCAAACCCC

Product: heavy metal efflux system periplasmic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MSHKSSQKLLVSALLLALGAGGGYWAAKQTGDGATAKEKTPLYWVNPMDPRDKRDGPAKDNMGMDFIPVYEEQKSGSPGT
VTISPEIQQNLGVRLAKVERLPIHQQIETVGYVGYDEDRLEAVNARMAGWIRTLAIKSEGQKVAKGSLLYEIYAPDLVNA
QHEYLLALNTTNPLLLLRAAEGKLKSLQVPADQIAALKRNRQVRETIGIYAPSSGYVSELKVREGQYVEPAAALFNISTL
QQVWVSAEVFERQAAQLKVGDPVTMTLDYAPGRSWQGRVDYLYPTLDAATRTLKVRLRVDNPDEFLKPNMFAKVAIRTGQ
GEPRLVVPSEAVIRTGSQDRLVLALGDGNFKSVAVTLGSQFGDKVAIKAGVETGDSIVSSAQFLLDSESAIDSDFQRMTA
VRPAQVWTQGTVQSMDLASRTLMVAHPPIPEWQWPAMEMEFTVAEGVDISKLAEGQTLHLQVIQEGDEYRITTIHQESAK
ESAPATGHAPEQATDEMEGMDHSQHGMGAKP

Sequences:

>Translated_511_residues
MSHKSSQKLLVSALLLALGAGGGYWAAKQTGDGATAKEKTPLYWVNPMDPRDKRDGPAKDNMGMDFIPVYEEQKSGSPGT
VTISPEIQQNLGVRLAKVERLPIHQQIETVGYVGYDEDRLEAVNARMAGWIRTLAIKSEGQKVAKGSLLYEIYAPDLVNA
QHEYLLALNTTNPLLLLRAAEGKLKSLQVPADQIAALKRNRQVRETIGIYAPSSGYVSELKVREGQYVEPAAALFNISTL
QQVWVSAEVFERQAAQLKVGDPVTMTLDYAPGRSWQGRVDYLYPTLDAATRTLKVRLRVDNPDEFLKPNMFAKVAIRTGQ
GEPRLVVPSEAVIRTGSQDRLVLALGDGNFKSVAVTLGSQFGDKVAIKAGVETGDSIVSSAQFLLDSESAIDSDFQRMTA
VRPAQVWTQGTVQSMDLASRTLMVAHPPIPEWQWPAMEMEFTVAEGVDISKLAEGQTLHLQVIQEGDEYRITTIHQESAK
ESAPATGHAPEQATDEMEGMDHSQHGMGAKP
>Mature_510_residues
SHKSSQKLLVSALLLALGAGGGYWAAKQTGDGATAKEKTPLYWVNPMDPRDKRDGPAKDNMGMDFIPVYEEQKSGSPGTV
TISPEIQQNLGVRLAKVERLPIHQQIETVGYVGYDEDRLEAVNARMAGWIRTLAIKSEGQKVAKGSLLYEIYAPDLVNAQ
HEYLLALNTTNPLLLLRAAEGKLKSLQVPADQIAALKRNRQVRETIGIYAPSSGYVSELKVREGQYVEPAAALFNISTLQ
QVWVSAEVFERQAAQLKVGDPVTMTLDYAPGRSWQGRVDYLYPTLDAATRTLKVRLRVDNPDEFLKPNMFAKVAIRTGQG
EPRLVVPSEAVIRTGSQDRLVLALGDGNFKSVAVTLGSQFGDKVAIKAGVETGDSIVSSAQFLLDSESAIDSDFQRMTAV
RPAQVWTQGTVQSMDLASRTLMVAHPPIPEWQWPAMEMEFTVAEGVDISKLAEGQTLHLQVIQEGDEYRITTIHQESAKE
SAPATGHAPEQATDEMEGMDHSQHGMGAKP

Specific function: Part of a cation efflux system that mediates resistance to copper and silver [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786787, Length=386, Percent_Identity=34.9740932642487, Blast_Score=198, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 55894; Mature: 55763

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKSSQKLLVSALLLALGAGGGYWAAKQTGDGATAKEKTPLYWVNPMDPRDKRDGPAKD
CCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
NMGMDFIPVYEEQKSGSPGTVTISPEIQQNLGVRLAKVERLPIHQQIETVGYVGYDEDRL
CCCCCEEEEECCCCCCCCCEEEECCHHHHCCCCEEEEEECCCCHHHHHHHEECCCCHHHH
EAVNARMAGWIRTLAIKSEGQKVAKGSLLYEIYAPDLVNAQHEYLLALNTTNPLLLLRAA
HHHHHHHHHHHEEEEECCCCCEECCCCEEEEEECCCCCCCCCEEEEEEECCCCEEEEEEC
EGKLKSLQVPADQIAALKRNRQVRETIGIYAPSSGYVSELKVREGQYVEPAAALFNISTL
CCCCEEECCCHHHHHHHHHCCHHHHHHEEECCCCCCHHHEEECCCCCCCHHHHHHHHHHH
QQVWVSAEVFERQAAQLKVGDPVTMTLDYAPGRSWQGRVDYLYPTLDAATRTLKVRLRVD
HHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCCEEEEECCHHCCCEEEEEEEEEC
NPDEFLKPNMFAKVAIRTGQGEPRLVVPSEAVIRTGSQDRLVLALGDGNFKSVAVTLGSQ
CCHHHHCCCCEEEEEEECCCCCCEEECCCHHHHCCCCCCEEEEEECCCCCEEEEEEECCC
FGDKVAIKAGVETGDSIVSSAQFLLDSESAIDSDFQRMTAVRPAQVWTQGTVQSMDLASR
CCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCCCHHHHHHCCC
TLMVAHPPIPEWQWPAMEMEFTVAEGVDISKLAEGQTLHLQVIQEGDEYRITTIHQESAK
EEEEECCCCCCCCCCCEEEEEEEECCCCHHHHCCCCEEEEEEEECCCCEEEEEEECHHHH
ESAPATGHAPEQATDEMEGMDHSQHGMGAKP
HCCCCCCCCCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SHKSSQKLLVSALLLALGAGGGYWAAKQTGDGATAKEKTPLYWVNPMDPRDKRDGPAKD
CCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
NMGMDFIPVYEEQKSGSPGTVTISPEIQQNLGVRLAKVERLPIHQQIETVGYVGYDEDRL
CCCCCEEEEECCCCCCCCCEEEECCHHHHCCCCEEEEEECCCCHHHHHHHEECCCCHHHH
EAVNARMAGWIRTLAIKSEGQKVAKGSLLYEIYAPDLVNAQHEYLLALNTTNPLLLLRAA
HHHHHHHHHHHEEEEECCCCCEECCCCEEEEEECCCCCCCCCEEEEEEECCCCEEEEEEC
EGKLKSLQVPADQIAALKRNRQVRETIGIYAPSSGYVSELKVREGQYVEPAAALFNISTL
CCCCEEECCCHHHHHHHHHCCHHHHHHEEECCCCCCHHHEEECCCCCCCHHHHHHHHHHH
QQVWVSAEVFERQAAQLKVGDPVTMTLDYAPGRSWQGRVDYLYPTLDAATRTLKVRLRVD
HHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCCEEEEECCHHCCCEEEEEEEEEC
NPDEFLKPNMFAKVAIRTGQGEPRLVVPSEAVIRTGSQDRLVLALGDGNFKSVAVTLGSQ
CCHHHHCCCCEEEEEEECCCCCCEEECCCHHHHCCCCCCEEEEEECCCCCEEEEEEECCC
FGDKVAIKAGVETGDSIVSSAQFLLDSESAIDSDFQRMTAVRPAQVWTQGTVQSMDLASR
CCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCCCHHHHHHCCC
TLMVAHPPIPEWQWPAMEMEFTVAEGVDISKLAEGQTLHLQVIQEGDEYRITTIHQESAK
EEEEECCCCCCCCCCCEEEEEEEECCCCHHHHCCCCEEEEEEEECCCCEEEEEEECHHHH
ESAPATGHAPEQATDEMEGMDHSQHGMGAKP
HCCCCCCCCCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]