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The map label for this gene is manA [H]

Identifier: 145299855

GI number: 145299855

Start: 3164719

End: 3165918

Strand: Direct

Name: manA [H]

Synonym: ASA_2944

Alternate gene names: 145299855

Gene position: 3164719-3165918 (Clockwise)

Preceding gene: 145299852

Following gene: 145299857

Centisome position: 67.3

GC content: 63.5

Gene sequence:

>1200_bases
ATGAGCAGTCGCGATCTGCCCTCTTTTTTGTTAATGCAAAATCCGATTCAAGGTTACGACTGGGGCAGCCATGACTCCCT
CACCACACTGTTTGGCATCCCAAATCCAACGGGCAAACCCCAGGCCGAACTGTGGATGGGCGCCCACCCCAACGGCTGCT
CCGAGGTGACCCTGGTGGGCAGTACGCAGAAGCTCTCGGCGGTGATCGACACAGCCCCGGCCGCCGCGCTGGGTGAGGCC
ACTGTCACCCACTTCGGCAGCCTGCCATTCCTGTTCAAGGTGCTGTGCGCCGAGAAGGCCCTCTCCATCCAGGTGCACCC
GAGCAAGGCCCAGGCCGAAGCTGGCTTTGCCAAAGAAGAAGCCGCTGGCATCCCCATCAAGGCGGGCAATCGCAACTACA
AAGACCCCAATCACAAGCCGGAGCTGGTGTTTGCCCTCACCGCCTATCAGGCGATGAACGGCTTTCGCGCCATTCCCGCC
ATCCTGGCGCTGTTCGAGCGGGTCAAGCTGGCGGCCATCGCCGATCTGGTCGCGGCCTTTGCCGCCAACCAGAACGAAGC
GGGCCTGCAGCACTTCTTCCACAAGCTGCTGATTTTACAAGGTGCGCGCAAGGAAGACGCCCTGACGGGCCTGCTGACCT
ATGCCAGCGCGCATCAGGATGAGGAGACCTTTGCCCTCATCACCAACCTGGCCGCCCAGTACCCGGGGGACGTGGGGCTC
TTCTCGCCGCTCTTGCTCAACGTGGTGACCTTGCAGCCGGGTCAGGCCATGTTCCTCGATGCCTGCACCCCCCACGCCTA
TGTGCGCGGCACTGGCCTCGAGATCATGGCCAACTCGGACAACGTGCTGCGCGCCGGTCTCACCCCCAAATACATAGACG
TGGCAGAGCTGTTGGATTGTACTCGCTGCCTGCCCAAGCCGGATGACCAGATCCTGCTTGCCCCCCGCGTGGAAGGGGCA
GTGCAGCACTTCGATGAGCCGGTACCGGACTTCACCTTCAGCGTCTATCCGGCAGGCGAGCATGGGCTCACCACCGCCAG
CGCCGAGATCCTGTTTGCCATCGATGGCACAGTGACGCTCAAGCAGGGCGACGAGACGCTGCGCCTCGAGAAGGGCCAGT
CCGCCTTTGTCCCCGCCGCCACCGGCCACTACCAGCTGCTGGCCGAAGGCCGGGTCGCCCGCGCCGGCAACCGCTGCTAA

Upstream 100 bases:

>100_bases
GTTGCGGCGGCCACAACCGCCCATGTTATAGTCCGGCCCACAGCAGCAATGCCACCCTTTTCTTCACCCGACTTTCTTCA
CACTAAATGAGGCATCCCTT

Downstream 100 bases:

>100_bases
GGCGTGAACCCGGCACGGGTTCAATGACTGAATAAACGCACAAGGGCCATCACTGATGGCCCTTGTTGTTTTTCTTTGGA
AATCATCTCCACCGTTAGTC

Product: mannose-6-phosphate isomerase

Products: NA

Alternate protein names: Phosphohexomutase; Phosphomannose isomerase; PMI [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSSRDLPSFLLMQNPIQGYDWGSHDSLTTLFGIPNPTGKPQAELWMGAHPNGCSEVTLVGSTQKLSAVIDTAPAAALGEA
TVTHFGSLPFLFKVLCAEKALSIQVHPSKAQAEAGFAKEEAAGIPIKAGNRNYKDPNHKPELVFALTAYQAMNGFRAIPA
ILALFERVKLAAIADLVAAFAANQNEAGLQHFFHKLLILQGARKEDALTGLLTYASAHQDEETFALITNLAAQYPGDVGL
FSPLLLNVVTLQPGQAMFLDACTPHAYVRGTGLEIMANSDNVLRAGLTPKYIDVAELLDCTRCLPKPDDQILLAPRVEGA
VQHFDEPVPDFTFSVYPAGEHGLTTASAEILFAIDGTVTLKQGDETLRLEKGQSAFVPAATGHYQLLAEGRVARAGNRC

Sequences:

>Translated_399_residues
MSSRDLPSFLLMQNPIQGYDWGSHDSLTTLFGIPNPTGKPQAELWMGAHPNGCSEVTLVGSTQKLSAVIDTAPAAALGEA
TVTHFGSLPFLFKVLCAEKALSIQVHPSKAQAEAGFAKEEAAGIPIKAGNRNYKDPNHKPELVFALTAYQAMNGFRAIPA
ILALFERVKLAAIADLVAAFAANQNEAGLQHFFHKLLILQGARKEDALTGLLTYASAHQDEETFALITNLAAQYPGDVGL
FSPLLLNVVTLQPGQAMFLDACTPHAYVRGTGLEIMANSDNVLRAGLTPKYIDVAELLDCTRCLPKPDDQILLAPRVEGA
VQHFDEPVPDFTFSVYPAGEHGLTTASAEILFAIDGTVTLKQGDETLRLEKGQSAFVPAATGHYQLLAEGRVARAGNRC
>Mature_398_residues
SSRDLPSFLLMQNPIQGYDWGSHDSLTTLFGIPNPTGKPQAELWMGAHPNGCSEVTLVGSTQKLSAVIDTAPAAALGEAT
VTHFGSLPFLFKVLCAEKALSIQVHPSKAQAEAGFAKEEAAGIPIKAGNRNYKDPNHKPELVFALTAYQAMNGFRAIPAI
LALFERVKLAAIADLVAAFAANQNEAGLQHFFHKLLILQGARKEDALTGLLTYASAHQDEETFALITNLAAQYPGDVGLF
SPLLLNVVTLQPGQAMFLDACTPHAYVRGTGLEIMANSDNVLRAGLTPKYIDVAELLDCTRCLPKPDDQILLAPRVEGAV
QHFDEPVPDFTFSVYPAGEHGLTTASAEILFAIDGTVTLKQGDETLRLEKGQSAFVPAATGHYQLLAEGRVARAGNRC

Specific function: Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide [H]

COG id: COG1482

COG function: function code G; Phosphomannose isomerase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 1 family [H]

Homologues:

Organism=Homo sapiens, GI4505235, Length=375, Percent_Identity=34.9333333333333, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI1787899, Length=390, Percent_Identity=49.7435897435897, Blast_Score=352, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI71997620, Length=404, Percent_Identity=31.6831683168317, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17557650, Length=413, Percent_Identity=27.1186440677966, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320839, Length=407, Percent_Identity=31.4496314496315, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI21356061, Length=355, Percent_Identity=33.8028169014084, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR001250
- InterPro:   IPR016305
- InterPro:   IPR018050
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01238 PMI_typeI [H]

EC number: =5.3.1.8 [H]

Molecular weight: Translated: 42716; Mature: 42585

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00965 PMI_I_1 ; PS00966 PMI_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRDLPSFLLMQNPIQGYDWGSHDSLTTLFGIPNPTGKPQAELWMGAHPNGCSEVTLVG
CCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEC
STQKLSAVIDTAPAAALGEATVTHFGSLPFLFKVLCAEKALSIQVHPSKAQAEAGFAKEE
CHHHHHHHHHCCCCHHHCCHHHHHCCCHHHHHHHHHHHHHEEEEECCCHHHHHCCCCHHH
AAGIPIKAGNRNYKDPNHKPELVFALTAYQAMNGFRAIPAILALFERVKLAAIADLVAAF
HCCCCEECCCCCCCCCCCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AANQNEAGLQHFFHKLLILQGARKEDALTGLLTYASAHQDEETFALITNLAAQYPGDVGL
HCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCH
FSPLLLNVVTLQPGQAMFLDACTPHAYVRGTGLEIMANSDNVLRAGLTPKYIDVAELLDC
HHHHHHEEEEECCCCEEEEECCCCCEEEECCCEEEEECCCCEEEECCCCCHHHHHHHHHH
TRCLPKPDDQILLAPRVEGAVQHFDEPVPDFTFSVYPAGEHGLTTASAEILFAIDGTVTL
HHCCCCCCCCEEEECCCHHHHHHCCCCCCCCEEEEEECCCCCCEECCCEEEEEECCEEEE
KQGDETLRLEKGQSAFVPAATGHYQLLAEGRVARAGNRC
ECCCCEEEEECCCCEECCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SSRDLPSFLLMQNPIQGYDWGSHDSLTTLFGIPNPTGKPQAELWMGAHPNGCSEVTLVG
CCCCCCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEC
STQKLSAVIDTAPAAALGEATVTHFGSLPFLFKVLCAEKALSIQVHPSKAQAEAGFAKEE
CHHHHHHHHHCCCCHHHCCHHHHHCCCHHHHHHHHHHHHHEEEEECCCHHHHHCCCCHHH
AAGIPIKAGNRNYKDPNHKPELVFALTAYQAMNGFRAIPAILALFERVKLAAIADLVAAF
HCCCCEECCCCCCCCCCCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AANQNEAGLQHFFHKLLILQGARKEDALTGLLTYASAHQDEETFALITNLAAQYPGDVGL
HCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCH
FSPLLLNVVTLQPGQAMFLDACTPHAYVRGTGLEIMANSDNVLRAGLTPKYIDVAELLDC
HHHHHHEEEEECCCCEEEEECCCCCEEEECCCEEEEECCCCEEEECCCCCHHHHHHHHHH
TRCLPKPDDQILLAPRVEGAVQHFDEPVPDFTFSVYPAGEHGLTTASAEILFAIDGTVTL
HHCCCCCCCCEEEECCCHHHHHHCCCCCCCCEEEEEECCCCCCEECCCEEEEEECCEEEE
KQGDETLRLEKGQSAFVPAATGHYQLLAEGRVARAGNRC
ECCCCEEEEECCCCEECCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6397402; 9097039; 9278503 [H]