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The map label for this gene is insF1 [H]

Identifier: 145299755

GI number: 145299755

Start: 3036008

End: 3036787

Strand: Direct

Name: insF1 [H]

Synonym: ASA_2832

Alternate gene names: 145299755

Gene position: 3036008-3036787 (Clockwise)

Preceding gene: 145299754

Following gene: 145299756

Centisome position: 64.56

GC content: 54.36

Gene sequence:

>780_bases
TTGAAACAGCCCGCGTCGCCACGCCAGCAGGAGGACGAACGACTGACCAGATTGGTCAAGCAATCCTGGCTGGAAAGTGG
TGCTGTCTACGGTTATCGCAAGATCCATCGTGACCTTCGTGACTGGGGGGAAGCCTGCGGCAAGCACCGTGTGGCCAAAT
TAATGAGGCGAGAAGGACTGCGTTCGCAAACCGGCTATCAGCGCCGTAAGGGGCATTATGGGGGTAAGAGCCTGCAGGCT
GCTCCGAACATGCGGGCTCGTCAGTTTAAGACCCCAGCCCCCAATATCTCCTGGGTGACCGACATTACCTACATCCGCAC
TCAAGAAGGGTGGCTGTATCTGGCTATAGTGCTAGATCTGTTTTCTCGCCAGATCGTCGGCTGGGCGATGAAGTCACGCA
TGACGGCAGACTTGGCGGTTGATGCGTTATGGATGGCGGTTTGGCGGCACAAGCCAAAGCAGCCGGTACTGGTGCATTCG
GATCAGGGCAGTCAGTTTACCGGTGGTGAATGGCAGGACTTTCTGAAGGCCCACAACCTGAGTTGCAGTATGAGCCTACG
TGGTAACTGTCACGACAACGCTGTCGCCGAGAGCTTCTTTCAGTTACTCAAACGGGAGCGGATCAAGCGCAGGATTTACC
CGACAAAAGACGAAGCCCGCAGCGACGTATTTGATTATATCGAGATGTTTTACAACCCAATCAGGCGCCATGGTTCCAAT
GATGGGCTGTCGCCGGTAGAGTTTGAAAAGCAGTTCAATGTTCAACAACAGAGTGTCTAG

Upstream 100 bases:

>100_bases
CAAGGAAAGGTACGCGTTCATAACCTCCCGACTCGACCGGTACAGTGTCGTTACCCTGTGCCAGACCCCGGGGGTTCACC
GAAGTGGCTTCTATGCGTGG

Downstream 100 bases:

>100_bases
AAAACCCGGGGCGATTCACTGGCATCCAAACAGCCGGGCGCACACCGCTATAAGGTTGCACGGTCTGAACGACCGGACAT
TACCAATCTGTTGGCACGTG

Product: IS3 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKQPASPRQQEDERLTRLVKQSWLESGAVYGYRKIHRDLRDWGEACGKHRVAKLMRREGLRSQTGYQRRKGHYGGKSLQA
APNMRARQFKTPAPNISWVTDITYIRTQEGWLYLAIVLDLFSRQIVGWAMKSRMTADLAVDALWMAVWRHKPKQPVLVHS
DQGSQFTGGEWQDFLKAHNLSCSMSLRGNCHDNAVAESFFQLLKRERIKRRIYPTKDEARSDVFDYIEMFYNPIRRHGSN
DGLSPVEFEKQFNVQQQSV

Sequences:

>Translated_259_residues
MKQPASPRQQEDERLTRLVKQSWLESGAVYGYRKIHRDLRDWGEACGKHRVAKLMRREGLRSQTGYQRRKGHYGGKSLQA
APNMRARQFKTPAPNISWVTDITYIRTQEGWLYLAIVLDLFSRQIVGWAMKSRMTADLAVDALWMAVWRHKPKQPVLVHS
DQGSQFTGGEWQDFLKAHNLSCSMSLRGNCHDNAVAESFFQLLKRERIKRRIYPTKDEARSDVFDYIEMFYNPIRRHGSN
DGLSPVEFEKQFNVQQQSV
>Mature_259_residues
MKQPASPRQQEDERLTRLVKQSWLESGAVYGYRKIHRDLRDWGEACGKHRVAKLMRREGLRSQTGYQRRKGHYGGKSLQA
APNMRARQFKTPAPNISWVTDITYIRTQEGWLYLAIVLDLFSRQIVGWAMKSRMTADLAVDALWMAVWRHKPKQPVLVHS
DQGSQFTGGEWQDFLKAHNLSCSMSLRGNCHDNAVAESFFQLLKRERIKRRIYPTKDEARSDVFDYIEMFYNPIRRHGSN
DGLSPVEFEKQFNVQQQSV

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=204, Percent_Identity=34.8039215686275, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI1788405, Length=246, Percent_Identity=42.6829268292683, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1787263, Length=246, Percent_Identity=42.6829268292683, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1786753, Length=246, Percent_Identity=42.6829268292683, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1786570, Length=246, Percent_Identity=42.6829268292683, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1786490, Length=246, Percent_Identity=42.6829268292683, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1789981, Length=251, Percent_Identity=32.2709163346614, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 30233; Mature: 30233

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQPASPRQQEDERLTRLVKQSWLESGAVYGYRKIHRDLRDWGEACGKHRVAKLMRREGL
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RSQTGYQRRKGHYGGKSLQAAPNMRARQFKTPAPNISWVTDITYIRTQEGWLYLAIVLDL
CHHCCHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCEEHHHEEEEEECCCHHHHHHHHHH
FSRQIVGWAMKSRMTADLAVDALWMAVWRHKPKQPVLVHSDQGSQFTGGEWQDFLKAHNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHCC
SCSMSLRGNCHDNAVAESFFQLLKRERIKRRIYPTKDEARSDVFDYIEMFYNPIRRHGSN
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DGLSPVEFEKQFNVQQQSV
CCCCCHHHHHHHCCHHCCC
>Mature Secondary Structure
MKQPASPRQQEDERLTRLVKQSWLESGAVYGYRKIHRDLRDWGEACGKHRVAKLMRREGL
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RSQTGYQRRKGHYGGKSLQAAPNMRARQFKTPAPNISWVTDITYIRTQEGWLYLAIVLDL
CHHCCHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCEEHHHEEEEEECCCHHHHHHHHHH
FSRQIVGWAMKSRMTADLAVDALWMAVWRHKPKQPVLVHSDQGSQFTGGEWQDFLKAHNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHCC
SCSMSLRGNCHDNAVAESFFQLLKRERIKRRIYPTKDEARSDVFDYIEMFYNPIRRHGSN
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DGLSPVEFEKQFNVQQQSV
CCCCCHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]