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The map label for this gene is yfiY [H]

Identifier: 145297067

GI number: 145297067

Start: 3288470

End: 3289498

Strand: Reverse

Name: yfiY [H]

Synonym: cgR_2963

Alternate gene names: 145297067

Gene position: 3289498-3288470 (Counterclockwise)

Preceding gene: 145297071

Following gene: 145297064

Centisome position: 99.26

GC content: 58.02

Gene sequence:

>1029_bases
ATGCAATCTATTCGGACGACCTTCAGGTCGCTTTCACGCAGCGCAGTTATCGTGGGTACCGTTTTGGCTCTGGGCATCGC
AGGATGTTCCACGGCCAGTGATGAAGCCACAAGCACCAGCGACGATGTAGCTGTCGGCGGCCGCCTATTTTCTACAGCGG
ATTCCGCCACCGCCGCTCTGGGAAGCGACGCCGAACCAGGCCAATTCCCCCGCACCGTTGTGCATTCACGCGGCGAAACT
ACCCTTGAGCAGCAGCCACAACGAGTCGTGGTTCTCGACAGCGGGGAAATCGATCAGGTTTTGAGCCTCGGCGTGACTCC
CGTCGGCATCGCCAGCCCGAAAGACGCCTCCAGCCAGCCCGCTTACCTCAAAGATCAGCTGGCAGACGTACAAACTGTAG
GCACCACGAGTGAGCTCAATTTCGAAGCCATCGCCGCCCTCAAGCCTGACCTGATTCTAGGCAGCAAGCTGCGAGTCGAC
GAATCCTACGATCAGCTCTCCCAAATCGCACCAACCGTGCTGAGTATTCGCCCCGGATTCCCATGGAAGGAAAACTTCCT
TTTCACCGCCGACGCGCTCGGCCTCGAGGGCAAAGCCGTCGAGGTTCTCAACGAGTACCAAACCCACGTCGATGCAGTCC
GCGAGACCATCGACGGCAGCCCAGAAATCTCACTCGTCCGCTTCATGCCCGGCCGCACCCGCCTGTACGGAAACCTCTCC
TTCATCGGTGTAATCCTTAAGGACCTGGGGCTTTCCCGCCCAGAGATCCAAAATATCGACGATCTTGCCGTGGAGATCTC
CCCCGAAAACATCGCCGACGCCAACGGCGACTGGATTTTCTACTCCACCTACGGCAAGCCCGAGGCCACCGAACAAGACA
ACATTTTGTCCAACGAGCTATGGCACAATCTCCCCGCCGTCCAGGACGGCAAGGCTTTGGAAGTAAATGACGAAAGCTGG
TTCATGGGGTTGGGTCCCCTCGGCGCCAATGAAGTGCTCAACGACATTGAAAACATCCTCGGATCCTAG

Upstream 100 bases:

>100_bases
CGACCGAGCAGTAAACTTGCTGGTCAGTGCAACTTTTTAACCGTAGAAAGTTATCGCCAAAGACTGGTAATAGTTTAAGA
AGCTGAGTTAGGATAGCCTA

Downstream 100 bases:

>100_bases
CCCCTCGACCCTGCAATTGAGCTGGTCAGCGCCTTGCGGATTGTGCCGCGCTTAATTGCTTATCGACGTCCCCCCTCGCC
CTCGCCGATGCTTCGGCGAG

Product: hypothetical protein

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MQSIRTTFRSLSRSAVIVGTVLALGIAGCSTASDEATSTSDDVAVGGRLFSTADSATAALGSDAEPGQFPRTVVHSRGET
TLEQQPQRVVVLDSGEIDQVLSLGVTPVGIASPKDASSQPAYLKDQLADVQTVGTTSELNFEAIAALKPDLILGSKLRVD
ESYDQLSQIAPTVLSIRPGFPWKENFLFTADALGLEGKAVEVLNEYQTHVDAVRETIDGSPEISLVRFMPGRTRLYGNLS
FIGVILKDLGLSRPEIQNIDDLAVEISPENIADANGDWIFYSTYGKPEATEQDNILSNELWHNLPAVQDGKALEVNDESW
FMGLGPLGANEVLNDIENILGS

Sequences:

>Translated_342_residues
MQSIRTTFRSLSRSAVIVGTVLALGIAGCSTASDEATSTSDDVAVGGRLFSTADSATAALGSDAEPGQFPRTVVHSRGET
TLEQQPQRVVVLDSGEIDQVLSLGVTPVGIASPKDASSQPAYLKDQLADVQTVGTTSELNFEAIAALKPDLILGSKLRVD
ESYDQLSQIAPTVLSIRPGFPWKENFLFTADALGLEGKAVEVLNEYQTHVDAVRETIDGSPEISLVRFMPGRTRLYGNLS
FIGVILKDLGLSRPEIQNIDDLAVEISPENIADANGDWIFYSTYGKPEATEQDNILSNELWHNLPAVQDGKALEVNDESW
FMGLGPLGANEVLNDIENILGS
>Mature_342_residues
MQSIRTTFRSLSRSAVIVGTVLALGIAGCSTASDEATSTSDDVAVGGRLFSTADSATAALGSDAEPGQFPRTVVHSRGET
TLEQQPQRVVVLDSGEIDQVLSLGVTPVGIASPKDASSQPAYLKDQLADVQTVGTTSELNFEAIAALKPDLILGSKLRVD
ESYDQLSQIAPTVLSIRPGFPWKENFLFTADALGLEGKAVEVLNEYQTHVDAVRETIDGSPEISLVRFMPGRTRLYGNLS
FIGVILKDLGLSRPEIQNIDDLAVEISPENIADANGDWIFYSTYGKPEATEQDNILSNELWHNLPAVQDGKALEVNDESW
FMGLGPLGANEVLNDIENILGS

Specific function: Part of the ABC transporter complex yfiYZ/yfhA/yusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen. Binds the siderophores and delivers them to the surface of yfiZ/yfhA (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable). Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082411, Length=275, Percent_Identity=28.7272727272727, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1786807, Length=295, Percent_Identity=25.7627118644068, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 36649; Mature: 36649

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSIRTTFRSLSRSAVIVGTVLALGIAGCSTASDEATSTSDDVAVGGRLFSTADSATAAL
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCEEECCCCHHHHHC
GSDAEPGQFPRTVVHSRGETTLEQQPQRVVVLDSGEIDQVLSLGVTPVGIASPKDASSQP
CCCCCCCCCCHHHHHCCCCCCHHCCCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCC
AYLKDQLADVQTVGTTSELNFEAIAALKPDLILGSKLRVDESYDQLSQIAPTVLSIRPGF
HHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEECCEECCCCHHHHHHHHCCCEEEECCCC
PWKENFLFTADALGLEGKAVEVLNEYQTHVDAVRETIDGSPEISLVRFMPGRTRLYGNLS
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECHH
FIGVILKDLGLSRPEIQNIDDLAVEISPENIADANGDWIFYSTYGKPEATEQDNILSNEL
HHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHH
WHNLPAVQDGKALEVNDESWFMGLGPLGANEVLNDIENILGS
HHCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQSIRTTFRSLSRSAVIVGTVLALGIAGCSTASDEATSTSDDVAVGGRLFSTADSATAAL
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCEEECCCCHHHHHC
GSDAEPGQFPRTVVHSRGETTLEQQPQRVVVLDSGEIDQVLSLGVTPVGIASPKDASSQP
CCCCCCCCCCHHHHHCCCCCCHHCCCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCC
AYLKDQLADVQTVGTTSELNFEAIAALKPDLILGSKLRVDESYDQLSQIAPTVLSIRPGF
HHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEECCEECCCCHHHHHHHHCCCEEEECCCC
PWKENFLFTADALGLEGKAVEVLNEYQTHVDAVRETIDGSPEISLVRFMPGRTRLYGNLS
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECHH
FIGVILKDLGLSRPEIQNIDDLAVEISPENIADANGDWIFYSTYGKPEATEQDNILSNEL
HHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHH
WHNLPAVQDGKALEVNDESWFMGLGPLGANEVLNDIENILGS
HHCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]