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The map label for this gene is proP [H]

Identifier: 145297063

GI number: 145297063

Start: 3283007

End: 3284683

Strand: Reverse

Name: proP [H]

Synonym: cgR_2959

Alternate gene names: 145297063

Gene position: 3284683-3283007 (Counterclockwise)

Preceding gene: 145297064

Following gene: 145297062

Centisome position: 99.11

GC content: 52.06

Gene sequence:

>1677_bases
ATGTTGAAAAGAGAGGTGGTGTTTTCACTTCCCTTTTATAGGCAAAGCTTTAAGGAGTTGTACAGGAAGAAGGAAACACC
GCCCAGGGGTGCGTTGGATGAAGATCATCTACAAACGAAGATTTCGTTATGCACTCATAAGATAAGACGAGAGGTTTTAC
TCGTGAGCCCGATTCGCTCAAAAAAGAAAATCAAAGACGAACCAAGACTAACAGTCGATGATGTAAACGTAGTCCCCCCA
AAGAAGATCCGCCCTGCCATTAAAGGCACTGTGGTGGGTAACTTTATGGAGTGGTACGACTTCGGTATTTATGGTTACCT
GACGGTCACGATGACCGCAGTATTTACACAAGGCCTGCCTGAAGAATGGCAGCTGCTCGCTGTCATGTTTGGTTTCGCAG
TGTCCTACCTGGTACGTCCATTGGGTGGTTTGATTCTGGGGCCATTAGGTGACAAGGTTGGTCGCCAAAAGGTTTTGTAC
GTGACCATGGCGATGATGGCGATTTCCACCGCGCTGATTGGTGTGTTGCCGACGGCGGCGTCGATAGGCGCTTGGGCTTT
GATTCTGCTTTATCTGTTGAAGATGATTCAGGGCTTCTCCACTGGTGGTGAGTACGCTGGCGCGACAACCTACGTGGCTG
AGTTTGCTCCAGATCGCCGCAGAGGCTACTTCGGTTCCTTCCTTGATATGGGTTCCTACCTGGGCTTTGCTGCTGGCGCA
TCCGTCGTTGCGATCACCACATGGGTGACTACCCACTATTGGGGTGCAAGCGCTATGGAAGATTTCGGTTGGCGAATCCC
CTTCCTGACCGCCATCCCACTGGGAATTATTGCGGTGTACCTGCGTACTCGTATCCCAGAAACTCCTGCGTTTGAGAACA
ACCAGGACGAAGACGAGGCAGTGATTGAGAAGGATTCCGATGATCCTTACGCTCGCCTTGGTCTGATGGGTGTTATCCGC
CACCACTGGCGTCCACTGCTGATTGGTATTGCGATTGTGGCAGCGACCAACACTGCCGGTTACGCCCTGACCAGTTACAT
GCCCGTGTATCTAGAGGAGCAGATCGGTCTGCACTCCGCATCCGCTGCCGCTGTGACCGTGCCGATTCTCGTTGTTATGT
CCCTGCTGCTGCCATTTGTTGGTATGTGGAGTGACCGCGTGGGCCGCAAGCCTGTCTACGCGACTGCTGTTGCGGCAACG
CTGATCTTGATGGTTCCTGCCTTCTTGATCATGAACACCGGAACTATCGGCGCCGTGCTAATTGCACTGTCCATGGTTGC
TATTCCAACCGGTTTGTATGTGGCACTGTCTGCATCTGCATTGCCTGCGCTGTTCCCGACTGCGTCACGATTCTCCGGAA
TGGGTATTTCCTACAACATTTCCGTGTCGTTGTTCGGTGGTACAACCCCGCTGATTACCCAGTTCCTGTTGCAGAAGACT
GGCCTGGATATCGTTCCAGCGCTCTACATCATGTTCTTCTCTGCAATCGCAGGTGTGGCTCTGCTGTTCATGACTGAGTC
TTCACAGAAGCCGCTGCTTGGTTCATTCCCAACTGTGGAAACCAAGTCTGAGGCCGTGGAGATCGTGAAGAACCAGGACG
AGGATCCAAATATCGATCTCACCCACATGCCGTTTACTGATTCGGAAACCGCAGAACCCGAAAAGCAAAACGCATAA

Upstream 100 bases:

>100_bases
CCCGCTCACCTGCATGAGTGTGGGGTCTTTTTGCATTCTTCCAGCTCCCAGACTTGAAAACGATCTGACTTTTCACCCCG
AACCTTACTAAGGTCGATTC

Downstream 100 bases:

>100_bases
CCTTAAAGCCCGCTGCAAGGCGGGCTTTTTTGGTGGCTGGGGGCGTCGAAAAGCGCTTATCGACGATTCCTCCTACCGGA
ATCTCTCCCAATTTTCTTTG

Product: hypothetical protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MLKREVVFSLPFYRQSFKELYRKKETPPRGALDEDHLQTKISLCTHKIRREVLLVSPIRSKKKIKDEPRLTVDDVNVVPP
KKIRPAIKGTVVGNFMEWYDFGIYGYLTVTMTAVFTQGLPEEWQLLAVMFGFAVSYLVRPLGGLILGPLGDKVGRQKVLY
VTMAMMAISTALIGVLPTAASIGAWALILLYLLKMIQGFSTGGEYAGATTYVAEFAPDRRRGYFGSFLDMGSYLGFAAGA
SVVAITTWVTTHYWGASAMEDFGWRIPFLTAIPLGIIAVYLRTRIPETPAFENNQDEDEAVIEKDSDDPYARLGLMGVIR
HHWRPLLIGIAIVAATNTAGYALTSYMPVYLEEQIGLHSASAAAVTVPILVVMSLLLPFVGMWSDRVGRKPVYATAVAAT
LILMVPAFLIMNTGTIGAVLIALSMVAIPTGLYVALSASALPALFPTASRFSGMGISYNISVSLFGGTTPLITQFLLQKT
GLDIVPALYIMFFSAIAGVALLFMTESSQKPLLGSFPTVETKSEAVEIVKNQDEDPNIDLTHMPFTDSETAEPEKQNA

Sequences:

>Translated_558_residues
MLKREVVFSLPFYRQSFKELYRKKETPPRGALDEDHLQTKISLCTHKIRREVLLVSPIRSKKKIKDEPRLTVDDVNVVPP
KKIRPAIKGTVVGNFMEWYDFGIYGYLTVTMTAVFTQGLPEEWQLLAVMFGFAVSYLVRPLGGLILGPLGDKVGRQKVLY
VTMAMMAISTALIGVLPTAASIGAWALILLYLLKMIQGFSTGGEYAGATTYVAEFAPDRRRGYFGSFLDMGSYLGFAAGA
SVVAITTWVTTHYWGASAMEDFGWRIPFLTAIPLGIIAVYLRTRIPETPAFENNQDEDEAVIEKDSDDPYARLGLMGVIR
HHWRPLLIGIAIVAATNTAGYALTSYMPVYLEEQIGLHSASAAAVTVPILVVMSLLLPFVGMWSDRVGRKPVYATAVAAT
LILMVPAFLIMNTGTIGAVLIALSMVAIPTGLYVALSASALPALFPTASRFSGMGISYNISVSLFGGTTPLITQFLLQKT
GLDIVPALYIMFFSAIAGVALLFMTESSQKPLLGSFPTVETKSEAVEIVKNQDEDPNIDLTHMPFTDSETAEPEKQNA
>Mature_558_residues
MLKREVVFSLPFYRQSFKELYRKKETPPRGALDEDHLQTKISLCTHKIRREVLLVSPIRSKKKIKDEPRLTVDDVNVVPP
KKIRPAIKGTVVGNFMEWYDFGIYGYLTVTMTAVFTQGLPEEWQLLAVMFGFAVSYLVRPLGGLILGPLGDKVGRQKVLY
VTMAMMAISTALIGVLPTAASIGAWALILLYLLKMIQGFSTGGEYAGATTYVAEFAPDRRRGYFGSFLDMGSYLGFAAGA
SVVAITTWVTTHYWGASAMEDFGWRIPFLTAIPLGIIAVYLRTRIPETPAFENNQDEDEAVIEKDSDDPYARLGLMGVIR
HHWRPLLIGIAIVAATNTAGYALTSYMPVYLEEQIGLHSASAAAVTVPILVVMSLLLPFVGMWSDRVGRKPVYATAVAAT
LILMVPAFLIMNTGTIGAVLIALSMVAIPTGLYVALSASALPALFPTASRFSGMGISYNISVSLFGGTTPLITQFLLQKT
GLDIVPALYIMFFSAIAGVALLFMTESSQKPLLGSFPTVETKSEAVEIVKNQDEDPNIDLTHMPFTDSETAEPEKQNA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=487, Percent_Identity=39.4250513347023, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1788942, Length=443, Percent_Identity=28.6681715575621, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788292, Length=451, Percent_Identity=29.9334811529933, Blast_Score=175, Evalue=7e-45,
Organism=Escherichia coli, GI1789941, Length=443, Percent_Identity=28.4424379232506, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87082404, Length=368, Percent_Identity=23.0978260869565, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87082231, Length=411, Percent_Identity=23.1143552311436, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 60985; Mature: 60985

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKREVVFSLPFYRQSFKELYRKKETPPRGALDEDHLQTKISLCTHKIRREVLLVSPIRS
CCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCH
KKKIKDEPRLTVDDVNVVPPKKIRPAIKGTVVGNFMEWYDFGIYGYLTVTMTAVFTQGLP
HHHCCCCCCEEECCCCCCCCHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
EEWQLLAVMFGFAVSYLVRPLGGLILGPLGDKVGRQKVLYVTMAMMAISTALIGVLPTAA
HHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SIGAWALILLYLLKMIQGFSTGGEYAGATTYVAEFAPDRRRGYFGSFLDMGSYLGFAAGA
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHCCC
SVVAITTWVTTHYWGASAMEDFGWRIPFLTAIPLGIIAVYLRTRIPETPAFENNQDEDEA
CEEEEEHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
VIEKDSDDPYARLGLMGVIRHHWRPLLIGIAIVAATNTAGYALTSYMPVYLEEQIGLHSA
HHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHCCCCCC
SAAAVTVPILVVMSLLLPFVGMWSDRVGRKPVYATAVAATLILMVPAFLIMNTGTIGAVL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IALSMVAIPTGLYVALSASALPALFPTASRFSGMGISYNISVSLFGGTTPLITQFLLQKT
HHHHHHHHCCCHHEEEEHHHHHHHCCCHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHC
GLDIVPALYIMFFSAIAGVALLFMTESSQKPLLGSFPTVETKSEAVEIVKNQDEDPNIDL
CCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEE
THMPFTDSETAEPEKQNA
EECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKREVVFSLPFYRQSFKELYRKKETPPRGALDEDHLQTKISLCTHKIRREVLLVSPIRS
CCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCH
KKKIKDEPRLTVDDVNVVPPKKIRPAIKGTVVGNFMEWYDFGIYGYLTVTMTAVFTQGLP
HHHCCCCCCEEECCCCCCCCHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
EEWQLLAVMFGFAVSYLVRPLGGLILGPLGDKVGRQKVLYVTMAMMAISTALIGVLPTAA
HHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SIGAWALILLYLLKMIQGFSTGGEYAGATTYVAEFAPDRRRGYFGSFLDMGSYLGFAAGA
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHCCC
SVVAITTWVTTHYWGASAMEDFGWRIPFLTAIPLGIIAVYLRTRIPETPAFENNQDEDEA
CEEEEEHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
VIEKDSDDPYARLGLMGVIRHHWRPLLIGIAIVAATNTAGYALTSYMPVYLEEQIGLHSA
HHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHCCCCCC
SAAAVTVPILVVMSLLLPFVGMWSDRVGRKPVYATAVAATLILMVPAFLIMNTGTIGAVL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IALSMVAIPTGLYVALSASALPALFPTASRFSGMGISYNISVSLFGGTTPLITQFLLQKT
HHHHHHHHCCCHHEEEEHHHHHHHCCCHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHC
GLDIVPALYIMFFSAIAGVALLFMTESSQKPLLGSFPTVETKSEAVEIVKNQDEDPNIDL
CCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEE
THMPFTDSETAEPEKQNA
EECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]