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The map label for this gene is hipO [H]

Identifier: 145296046

GI number: 145296046

Start: 2165489

End: 2166754

Strand: Direct

Name: hipO [H]

Synonym: cgR_1968

Alternate gene names: 145296046

Gene position: 2165489-2166754 (Clockwise)

Preceding gene: 145296045

Following gene: 145296065

Centisome position: 65.34

GC content: 54.66

Gene sequence:

>1266_bases
ATGAGCCGCATTTCAGAACTTCTAAACAATCATGGTGTTGATCTGTCGTGGCAAGAGGCCGCATATCAGGATTTCCACGA
ACATCCTGAGCTCTCCGGCTTCGAATCAGAGACTGCAGATCGCATTCAGAAATACCTCGAGCGTTTTGATTGTGAGGTGA
TTCCGAATGTTGGCGGTTACGGCATTCTGGCGGTGTTCCGAAATGGATCGACAGATCCTGGTGCCCCTGTTGCGTTAATG
CGCGCAGATTTCGATGGCCTTCCCGTCAAGGAAATCACCGGAGTTCCGTTTGCTTCCACTCGTATGCGTCCGCATGATGG
GGCAAATGTCCATGTCATGCACGCATGCGGCCACGATGTCCACGTCACCGCGCTGCTTGGTGCGTGTGCCATTTTGGATG
AGCGTCGCGATGCATGGGAAGGCACGTTCATCGCGTTGTTCCAGCCATCGGAGGAAAACTCCCAAGGCGCTAACAAGATG
GTCGCCGGCGGTTTAGTTGATCTGATCCCACGCCCTGATGTGTGCTTTGGCCAGCATGTTGTCCCCGGAGCTGCAGGAAC
CGTGATGAGCATGCCTGGCGGTGCTCTCGCTGCCTGCGATTCCATTGAAATCCGCATTCAGGGTCGCAGCGCCCATGGTT
CCATGCCTCATAATTCCATCGATCCCACTTATGTTGCAGCGATGATTGTCGTGCGACTCCAAGGAATCGTGGGCCGCGAG
GTTTCTCCAGAGGATTTCGCCGTTATTTCTGTGGGCACCCTCCAGTCGGGAAACACCAACAACACCATTCCAGCAAGTGC
TCGTTTGGTGTTGAACTGCCGTTTCTACAACGACAAAGTCAAGCACAAGGTCTACCGAGCCATCGAACGTGTTGTCCGTG
GTGAATGCCTTGCTTCCGGTATTGAGGAAGAACCTGTCATTGAGTACTTCGCCCACGGTGATCTCACCGACAACACCCCT
GTTGTCTTCGATACTGTGCGCCCTGTGTTCGACGATGTTTTCGGCGAGGATTCTATTGATGCCTACCGGTGGACTGCGTC
GGAGGATTTCCCCTCCATTCCTAAGGCATTCAACAGCCCTTACCTGTACTGGACGATTGGTGTCACGCCGCGCGATCAGT
GGACAGAAGCCGTAGAAAGAGACCGCGTGGCATCGGATGTGCCAGCCAATCACATGGGAGATTTCCTCCCTGATTATGCT
CCGACGATGTCCGCTGCCACCCGCGCAGCCGCAGCCGCGCTGCTGACCTACTTGGGAACTAACTAA

Upstream 100 bases:

>100_bases
GCCATGGAATGCTCCGTTGAACGCAACAGCCTTAAATACAATCCCCTCCTATAAGCCAAGAGTTTCAGTGTCGCTGCGCA
GGTACTCTACTATCTAATCC

Downstream 100 bases:

>100_bases
TCATCTAGTTTTCTGCGACGACGGATTCGAAAAGAGACAACGGCAATGGTCAGCGCCACTCCAATGGCGATGAGGGCGAA
ATTAACTAGACCATAAAGCC

Product: hypothetical protein

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSRISELLNNHGVDLSWQEAAYQDFHEHPELSGFESETADRIQKYLERFDCEVIPNVGGYGILAVFRNGSTDPGAPVALM
RADFDGLPVKEITGVPFASTRMRPHDGANVHVMHACGHDVHVTALLGACAILDERRDAWEGTFIALFQPSEENSQGANKM
VAGGLVDLIPRPDVCFGQHVVPGAAGTVMSMPGGALAACDSIEIRIQGRSAHGSMPHNSIDPTYVAAMIVVRLQGIVGRE
VSPEDFAVISVGTLQSGNTNNTIPASARLVLNCRFYNDKVKHKVYRAIERVVRGECLASGIEEEPVIEYFAHGDLTDNTP
VVFDTVRPVFDDVFGEDSIDAYRWTASEDFPSIPKAFNSPYLYWTIGVTPRDQWTEAVERDRVASDVPANHMGDFLPDYA
PTMSAATRAAAAALLTYLGTN

Sequences:

>Translated_421_residues
MSRISELLNNHGVDLSWQEAAYQDFHEHPELSGFESETADRIQKYLERFDCEVIPNVGGYGILAVFRNGSTDPGAPVALM
RADFDGLPVKEITGVPFASTRMRPHDGANVHVMHACGHDVHVTALLGACAILDERRDAWEGTFIALFQPSEENSQGANKM
VAGGLVDLIPRPDVCFGQHVVPGAAGTVMSMPGGALAACDSIEIRIQGRSAHGSMPHNSIDPTYVAAMIVVRLQGIVGRE
VSPEDFAVISVGTLQSGNTNNTIPASARLVLNCRFYNDKVKHKVYRAIERVVRGECLASGIEEEPVIEYFAHGDLTDNTP
VVFDTVRPVFDDVFGEDSIDAYRWTASEDFPSIPKAFNSPYLYWTIGVTPRDQWTEAVERDRVASDVPANHMGDFLPDYA
PTMSAATRAAAAALLTYLGTN
>Mature_420_residues
SRISELLNNHGVDLSWQEAAYQDFHEHPELSGFESETADRIQKYLERFDCEVIPNVGGYGILAVFRNGSTDPGAPVALMR
ADFDGLPVKEITGVPFASTRMRPHDGANVHVMHACGHDVHVTALLGACAILDERRDAWEGTFIALFQPSEENSQGANKMV
AGGLVDLIPRPDVCFGQHVVPGAAGTVMSMPGGALAACDSIEIRIQGRSAHGSMPHNSIDPTYVAAMIVVRLQGIVGREV
SPEDFAVISVGTLQSGNTNNTIPASARLVLNCRFYNDKVKHKVYRAIERVVRGECLASGIEEEPVIEYFAHGDLTDNTPV
VFDTVRPVFDDVFGEDSIDAYRWTASEDFPSIPKAFNSPYLYWTIGVTPRDQWTEAVERDRVASDVPANHMGDFLPDYAP
TMSAATRAAAAALLTYLGTN

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=251, Percent_Identity=30.6772908366534, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 45837; Mature: 45706

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRISELLNNHGVDLSWQEAAYQDFHEHPELSGFESETADRIQKYLERFDCEVIPNVGGY
CHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCC
GILAVFRNGSTDPGAPVALMRADFDGLPVKEITGVPFASTRMRPHDGANVHVMHACGHDV
EEEEEEECCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCC
HVTALLGACAILDERRDAWEGTFIALFQPSEENSQGANKMVAGGLVDLIPRPDVCFGQHV
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCHHHHCCCCCCCCCCCC
VPGAAGTVMSMPGGALAACDSIEIRIQGRSAHGSMPHNSIDPTYVAAMIVVRLQGIVGRE
CCCCCCCEEECCCCCEEECCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
VSPEDFAVISVGTLQSGNTNNTIPASARLVLNCRFYNDKVKHKVYRAIERVVRGECLASG
CCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
IEEEPVIEYFAHGDLTDNTPVVFDTVRPVFDDVFGEDSIDAYRWTASEDFPSIPKAFNSP
CCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHCCC
YLYWTIGVTPRDQWTEAVERDRVASDVPANHMGDFLPDYAPTMSAATRAAAAALLTYLGT
EEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
N
C
>Mature Secondary Structure 
SRISELLNNHGVDLSWQEAAYQDFHEHPELSGFESETADRIQKYLERFDCEVIPNVGGY
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCC
GILAVFRNGSTDPGAPVALMRADFDGLPVKEITGVPFASTRMRPHDGANVHVMHACGHDV
EEEEEEECCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCC
HVTALLGACAILDERRDAWEGTFIALFQPSEENSQGANKMVAGGLVDLIPRPDVCFGQHV
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCHHHHCCCCCCCCCCCC
VPGAAGTVMSMPGGALAACDSIEIRIQGRSAHGSMPHNSIDPTYVAAMIVVRLQGIVGRE
CCCCCCCEEECCCCCEEECCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
VSPEDFAVISVGTLQSGNTNNTIPASARLVLNCRFYNDKVKHKVYRAIERVVRGECLASG
CCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
IEEEPVIEYFAHGDLTDNTPVVFDTVRPVFDDVFGEDSIDAYRWTASEDFPSIPKAFNSP
CCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHCCC
YLYWTIGVTPRDQWTEAVERDRVASDVPANHMGDFLPDYAPTMSAATRAAAAALLTYLGT
EEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]