Click here to switch to the map view.

The map label for this gene is gdh [H]

Identifier: 145296042

GI number: 145296042

Start: 2160420

End: 2161763

Strand: Reverse

Name: gdh [H]

Synonym: cgR_1964

Alternate gene names: 145296042

Gene position: 2161763-2160420 (Counterclockwise)

Preceding gene: 145296044

Following gene: 145296039

Centisome position: 65.23

GC content: 56.1

Gene sequence:

>1344_bases
ATGACAGTTGATGAGCAGGTCTCTAACTATTACGACATGCTTCTGAAACGCAATGCTGGCGAGCCTGAATTTCACCAGGC
AGTGGCAGAGGTTTTGGAATCTTTGAAGATCGTCCTGGAAAAGGACCCTCATTACGCTGATTACGGTCTCATCCAGCGCC
TGTGCGAGCCTGAGCGTCAGCTCATCTTCCGTGTTCCTTGGGTTGATGACCAGGGCCAGGTCCACGTCAACCGTGGTTTC
CGCGTGCAGTTCAACTCTGCACTTGGACCATACAAGGGCGGCCTGCGCTTCCACCCATCTGTAAACCTGGGCATTGTGAA
GTTCCTGGGCTTTGAGCAGATCTTTAAAAACTCCCTAACCGGCCTGCCAATCGGTGGTGGCAAGGGTGGATCCGACTTCG
ACCCTAAGGGCAAGTCCGATCTGGAAATCATGCGTTTCTGCCAGTCCTTCATGACCGAGCTGCACCGCCACATCGGTGAG
TACCGCGACGTTCCTGCAGGTGACATCGGAGTTGGTGGCCGCGAGATCGGTTACCTGTTTGGCCACTACCGTCGCATGGC
TAACCAGCACGAGTCCGGCGTTTTGACCGGTAAGGGCCTGACCTGGGGTGGATCCCTGGTCCGCACCGAGGCAACTGGTT
ACGGCTGCGTTTACTTCGTGAGTGAAATGATCAAGGCTAAGGGCGAGAGCATCAGCGGCCAGAAGATCATCGTTTCCGGT
TCCGGCAACGTAGCAACCTACGCGATTGAAAAGGCTCAGGAACTCGGCGCAACCGTTATTGGTTTCTCCGATTCCAGCGG
TTGGGTTCATACCCCTAACGGCGTTGACGTGGCTAAGCTCCGCGAAATCAAGGAAGTTCGCCGCGCACGCGTATCCGTGT
ACGCCGACGAAGTTGAAGGCGCAACCTACCACACCGACGGTTCCATCTGGGATCTCAAGTGCGATATCGCTCTTCCTTGT
GCAACTCAGAACGAGCTCAACGGCGAGAACGCTAAGACTCTTGCAGACAACGGCTGCCGTTTCGTTGCTGAAGGCGCGAA
CATGCCTTCCACCCCTGAGGCTGTTGAGGTCTTCCGTGAGCGCGACATCCGCTTCGGACCAGGCAAGGCAGCTAACGCTG
GTGGCGTTGCAACCTCCGCTCTGGAGATGCAGCAGAACGCTTCGCGCGATTCCTGGAGCTTCGAGTACACCGACGAGCGC
CTCCAGGTGATCATGAAGAACATCTTCAAGACCTGTGCAGAGACCGCAGCAGAGTATGGACACGAGAACGATTACGTTGT
CGGCGCTAACATTGCTGGCTTCAAGAAGGTAGCTGACGCGATGCTGGCACAGGGCGTCATCTAA

Upstream 100 bases:

>100_bases
TACATGGTGCCCTCAATGGGAACCACCAACATCACTAAATGGCCCAGATACACACTTTAAAATCGTGCGCGCATGCAGCC
GAGATGGGAACGAGGAAATC

Downstream 100 bases:

>100_bases
GACCCCTGCACTTTACTTAAACCCCTGATCCGCGTTAAGGATCAGGGATTTTTGATTTCTTCCAGGTCAATTATCCGATC
CACATGGGTTAATGCAGCTG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTVDEQVSNYYDMLLKRNAGEPEFHQAVAEVLESLKIVLEKDPHYADYGLIQRLCEPERQLIFRVPWVDDQGQVHVNRGF
RVQFNSALGPYKGGLRFHPSVNLGIVKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDLEIMRFCQSFMTELHRHIGE
YRDVPAGDIGVGGREIGYLFGHYRRMANQHESGVLTGKGLTWGGSLVRTEATGYGCVYFVSEMIKAKGESISGQKIIVSG
SGNVATYAIEKAQELGATVIGFSDSSGWVHTPNGVDVAKLREIKEVRRARVSVYADEVEGATYHTDGSIWDLKCDIALPC
ATQNELNGENAKTLADNGCRFVAEGANMPSTPEAVEVFRERDIRFGPGKAANAGGVATSALEMQQNASRDSWSFEYTDER
LQVIMKNIFKTCAETAAEYGHENDYVVGANIAGFKKVADAMLAQGVI

Sequences:

>Translated_447_residues
MTVDEQVSNYYDMLLKRNAGEPEFHQAVAEVLESLKIVLEKDPHYADYGLIQRLCEPERQLIFRVPWVDDQGQVHVNRGF
RVQFNSALGPYKGGLRFHPSVNLGIVKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDLEIMRFCQSFMTELHRHIGE
YRDVPAGDIGVGGREIGYLFGHYRRMANQHESGVLTGKGLTWGGSLVRTEATGYGCVYFVSEMIKAKGESISGQKIIVSG
SGNVATYAIEKAQELGATVIGFSDSSGWVHTPNGVDVAKLREIKEVRRARVSVYADEVEGATYHTDGSIWDLKCDIALPC
ATQNELNGENAKTLADNGCRFVAEGANMPSTPEAVEVFRERDIRFGPGKAANAGGVATSALEMQQNASRDSWSFEYTDER
LQVIMKNIFKTCAETAAEYGHENDYVVGANIAGFKKVADAMLAQGVI
>Mature_446_residues
TVDEQVSNYYDMLLKRNAGEPEFHQAVAEVLESLKIVLEKDPHYADYGLIQRLCEPERQLIFRVPWVDDQGQVHVNRGFR
VQFNSALGPYKGGLRFHPSVNLGIVKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDLEIMRFCQSFMTELHRHIGEY
RDVPAGDIGVGGREIGYLFGHYRRMANQHESGVLTGKGLTWGGSLVRTEATGYGCVYFVSEMIKAKGESISGQKIIVSGS
GNVATYAIEKAQELGATVIGFSDSSGWVHTPNGVDVAKLREIKEVRRARVSVYADEVEGATYHTDGSIWDLKCDIALPCA
TQNELNGENAKTLADNGCRFVAEGANMPSTPEAVEVFRERDIRFGPGKAANAGGVATSALEMQQNASRDSWSFEYTDERL
QVIMKNIFKTCAETAAEYGHENDYVVGANIAGFKKVADAMLAQGVI

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=367, Percent_Identity=31.3351498637602, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI4885281, Length=377, Percent_Identity=31.0344827586207, Blast_Score=156, Evalue=4e-38,
Organism=Escherichia coli, GI1788059, Length=444, Percent_Identity=58.3333333333333, Blast_Score=504, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17544676, Length=425, Percent_Identity=27.0588235294118, Blast_Score=136, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6319256, Length=458, Percent_Identity=51.9650655021834, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=51.5486725663717, Blast_Score=405, Evalue=1e-114,
Organism=Drosophila melanogaster, GI45553475, Length=411, Percent_Identity=29.1970802919708, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI45549226, Length=413, Percent_Identity=29.5399515738499, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24649283, Length=415, Percent_Identity=27.4698795180723, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24649460, Length=301, Percent_Identity=29.9003322259136, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48989; Mature: 48858

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDEQVSNYYDMLLKRNAGEPEFHQAVAEVLESLKIVLEKDPHYADYGLIQRLCEPERQ
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHH
LIFRVPWVDDQGQVHVNRGFRVQFNSALGPYKGGLRFHPSVNLGIVKFLGFEQIFKNSLT
EEEECCCCCCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCC
GLPIGGGKGGSDFDPKGKSDLEIMRFCQSFMTELHRHIGEYRDVPAGDIGVGGREIGYLF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
GHYRRMANQHESGVLTGKGLTWGGSLVRTEATGYGCVYFVSEMIKAKGESISGQKIIVSG
HHHHHHHCCCCCCEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEEC
SGNVATYAIEKAQELGATVIGFSDSSGWVHTPNGVDVAKLREIKEVRRARVSVYADEVEG
CCCEEEHHHHHHHHCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
ATYHTDGSIWDLKCDIALPCATQNELNGENAKTLADNGCRFVAEGANMPSTPEAVEVFRE
CEEECCCCEEEEEECEEECCCCCCCCCCCCCHHHHCCCCEEEECCCCCCCCHHHHHHHHH
RDIRFGPGKAANAGGVATSALEMQQNASRDSWSFEYTDERLQVIMKNIFKTCAETAAEYG
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHC
HENDYVVGANIAGFKKVADAMLAQGVI
CCCCEEEECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TVDEQVSNYYDMLLKRNAGEPEFHQAVAEVLESLKIVLEKDPHYADYGLIQRLCEPERQ
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHH
LIFRVPWVDDQGQVHVNRGFRVQFNSALGPYKGGLRFHPSVNLGIVKFLGFEQIFKNSLT
EEEECCCCCCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCC
GLPIGGGKGGSDFDPKGKSDLEIMRFCQSFMTELHRHIGEYRDVPAGDIGVGGREIGYLF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
GHYRRMANQHESGVLTGKGLTWGGSLVRTEATGYGCVYFVSEMIKAKGESISGQKIIVSG
HHHHHHHCCCCCCEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEEC
SGNVATYAIEKAQELGATVIGFSDSSGWVHTPNGVDVAKLREIKEVRRARVSVYADEVEG
CCCEEEHHHHHHHHCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
ATYHTDGSIWDLKCDIALPCATQNELNGENAKTLADNGCRFVAEGANMPSTPEAVEVFRE
CEEECCCCEEEEEECEEECCCCCCCCCCCCCHHHHCCCCEEEECCCCCCCCHHHHHHHHH
RDIRFGPGKAANAGGVATSALEMQQNASRDSWSFEYTDERLQVIMKNIFKTCAETAAEYG
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHC
HENDYVVGANIAGFKKVADAMLAQGVI
CCCCEEEECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]