Click here to switch to the map view.

The map label for this gene is cobB

Identifier: 145294812

GI number: 145294812

Start: 839096

End: 839872

Strand: Direct

Name: cobB

Synonym: cgR_0759

Alternate gene names: 145294812

Gene position: 839096-839872 (Clockwise)

Preceding gene: 145294810

Following gene: 145294813

Centisome position: 25.32

GC content: 55.6

Gene sequence:

>777_bases
ATGAGCGAACGTCAGCTGGAAAAGTCAATTGAGCACGCCGTCGAGTTAGCCCGCGAAGCCCGAAACATCGAAGTTTTTAC
CGGAGCCGGAATGAGCGCCGACTCCGGGTTGGAAACGTATCGTGATGATAAAACCGGGCTGTGGAGCAACGTAGATCCAC
AAGCGATGGCAAGTATCGATGCATGGCGCAAAGATCCAGAGCCAATGTGGGCGTGGTATCGCTGGCGCGCCGGGGTGGCA
GCTAGGGCAGAACCCAACGCGGGGCATCAAGCTATTTCCTACTGGGAGGGGAGTGACACCGTCGAACACGTTCACATCAC
CACCCAGAACATTGACAACCTGCACGAGCGAGCTGGCTCTAGCGATGTGACGCATCTTCATGGCAGCTTGTTTGAATACA
GGTGCTCTGATTGTGCGACTCCATGGGAAGACGATAAAAACTATCCACAAGAACCCATTGCACGCCTTGCTCCTCCACAA
TGTGAAAAGTGCGGAGGGCTGATTAGGCCAGGTGTGGTGTGGTTTGGTGAGAACCTGCCCGTAGAAGAGTGGGATATTGC
AGAGCAACGCATCGCAGAAGCCGATCTCATGATCATTGTGGGTACCTCCGGGATTGTTCATCCTGCAGCAGCACTCCCGC
AATTAGCCCAACAACGCGGCGTTCCCATCGTGGAGATCTCCCCAACGCGCACCGAACTTAGCCGGATCGCAGACTTCACC
TGGATGTCCACCGCAGCCCAAGCGCTACCAGCGTTGATGCGAGGTTTGAGCGCCTAA

Upstream 100 bases:

>100_bases
GAATAAACCAAAGGGTGGACACTTATTCGAACGGGTGCTTGATTTAGGCAACTTGAGCAAAATCCTGCCATTTCACCTCA
AATCGGGCTAGTTTTGAAGC

Downstream 100 bases:

>100_bases
CATGACTGAAGATGACTTAGATCTGCTGCACCGCACAGTAGAACTAGCCACCCAGGCACTCAAGCAGGGAAACAGTCCTT
ATGGATCCCTGCTGGTTGAT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSERQLEKSIEHAVELAREARNIEVFTGAGMSADSGLETYRDDKTGLWSNVDPQAMASIDAWRKDPEPMWAWYRWRAGVA
ARAEPNAGHQAISYWEGSDTVEHVHITTQNIDNLHERAGSSDVTHLHGSLFEYRCSDCATPWEDDKNYPQEPIARLAPPQ
CEKCGGLIRPGVVWFGENLPVEEWDIAEQRIAEADLMIIVGTSGIVHPAAALPQLAQQRGVPIVEISPTRTELSRIADFT
WMSTAAQALPALMRGLSA

Sequences:

>Translated_258_residues
MSERQLEKSIEHAVELAREARNIEVFTGAGMSADSGLETYRDDKTGLWSNVDPQAMASIDAWRKDPEPMWAWYRWRAGVA
ARAEPNAGHQAISYWEGSDTVEHVHITTQNIDNLHERAGSSDVTHLHGSLFEYRCSDCATPWEDDKNYPQEPIARLAPPQ
CEKCGGLIRPGVVWFGENLPVEEWDIAEQRIAEADLMIIVGTSGIVHPAAALPQLAQQRGVPIVEISPTRTELSRIADFT
WMSTAAQALPALMRGLSA
>Mature_257_residues
SERQLEKSIEHAVELAREARNIEVFTGAGMSADSGLETYRDDKTGLWSNVDPQAMASIDAWRKDPEPMWAWYRWRAGVAA
RAEPNAGHQAISYWEGSDTVEHVHITTQNIDNLHERAGSSDVTHLHGSLFEYRCSDCATPWEDDKNYPQEPIARLAPPQC
EKCGGLIRPGVVWFGENLPVEEWDIAEQRIAEADLMIIVGTSGIVHPAAALPQLAQQRGVPIVEISPTRTELSRIADFTW
MSTAAQALPALMRGLSA

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain

Homologues:

Organism=Homo sapiens, GI300795542, Length=237, Percent_Identity=38.3966244725738, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI6912664, Length=254, Percent_Identity=36.6141732283465, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI13787215, Length=231, Percent_Identity=38.5281385281385, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI6912662, Length=274, Percent_Identity=29.9270072992701, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI7657575, Length=224, Percent_Identity=29.9107142857143, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI13775602, Length=204, Percent_Identity=27.9411764705882, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI13775600, Length=201, Percent_Identity=28.3582089552239, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI300797705, Length=195, Percent_Identity=27.1794871794872, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI6912660, Length=229, Percent_Identity=28.3842794759825, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI63054862, Length=209, Percent_Identity=27.7511961722488, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI300797577, Length=261, Percent_Identity=23.7547892720307, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI7706712, Length=208, Percent_Identity=25.4807692307692, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI308199517, Length=249, Percent_Identity=34.136546184739, Blast_Score=121, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71990482, Length=261, Percent_Identity=31.0344827586207, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17541892, Length=227, Percent_Identity=29.9559471365639, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71990487, Length=263, Percent_Identity=30.7984790874525, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17567771, Length=264, Percent_Identity=28.4090909090909, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6325242, Length=212, Percent_Identity=28.3018867924528, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648389, Length=200, Percent_Identity=31, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI28571445, Length=277, Percent_Identity=24.187725631769, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI17137536, Length=238, Percent_Identity=26.0504201680672, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571443, Length=197, Percent_Identity=24.8730964467005, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI28571441, Length=197, Percent_Identity=24.8730964467005, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NPD_CORGL (Q8NSM4)

Other databases:

- EMBL:   BA000036
- EMBL:   BX927149
- RefSeq:   NP_599877.1
- RefSeq:   YP_224936.1
- ProteinModelPortal:   Q8NSM4
- SMR:   Q8NSM4
- GeneID:   1018645
- GeneID:   3345527
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg0745
- KEGG:   cgl:NCgl0616
- HOGENOM:   HBG641281
- OMA:   DADGLWE
- PhylomeDB:   Q8NSM4
- ProtClustDB:   PRK00481
- BioCyc:   CGLU196627:CG0745-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01121
- InterPro:   IPR003000
- PANTHER:   PTHR11085

Pfam domain/function: PF02146 SIR2

EC number: 3.5.1.- [C]

Molecular weight: Translated: 28628; Mature: 28497

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50305 SIRTUIN

Important sites: ACT_SITE 127-127

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERQLEKSIEHAVELAREARNIEVFTGAGMSADSGLETYRDDKTGLWSNVDPQAMASID
CCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHH
AWRKDPEPMWAWYRWRAGVAARAEPNAGHQAISYWEGSDTVEHVHITTQNIDNLHERAGS
HHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHEEEECCCCCEEEEEEEHHHHHHHHHHCCC
SDVTHLHGSLFEYRCSDCATPWEDDKNYPQEPIARLAPPQCEKCGGLIRPGVVWFGENLP
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHHHCCCCCCCCEEEECCCCC
VEEWDIAEQRIAEADLMIIVGTSGIVHPAAALPQLAQQRGVPIVEISPTRTELSRIADFT
CCHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
WMSTAAQALPALMRGLSA
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SERQLEKSIEHAVELAREARNIEVFTGAGMSADSGLETYRDDKTGLWSNVDPQAMASID
CHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHH
AWRKDPEPMWAWYRWRAGVAARAEPNAGHQAISYWEGSDTVEHVHITTQNIDNLHERAGS
HHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHEEEECCCCCEEEEEEEHHHHHHHHHHCCC
SDVTHLHGSLFEYRCSDCATPWEDDKNYPQEPIARLAPPQCEKCGGLIRPGVVWFGENLP
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHHHCCCCCCCCEEEECCCCC
VEEWDIAEQRIAEADLMIIVGTSGIVHPAAALPQLAQQRGVPIVEISPTRTELSRIADFT
CCHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
WMSTAAQALPALMRGLSA
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626