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The map label for this gene is tuf [H]

Identifier: 145294648

GI number: 145294648

Start: 666804

End: 667994

Strand: Direct

Name: tuf [H]

Synonym: cgR_0598

Alternate gene names: 145294648

Gene position: 666804-667994 (Clockwise)

Preceding gene: 145294647

Following gene: 145294649

Centisome position: 20.12

GC content: 56.51

Gene sequence:

>1191_bases
GTGGCAAAGGCGAAGTTCGAGCGTACCAAGCCCCACGTAAACATCGGCACCATCGGTCACGTTGACCACGGTAAGACCAC
CACCACCGCGGCTATCACCAAGGTTCTGGCTGACGCTTACCCTGAGCTCAACGAGGCTTTCGCCTTCGACTCCATCGATA
AGGCTCCTGAGGAGAAGGAGCGTGGCATCACGATCAACATCTCCCACGTTGAGTACCAGACCGAGAAGCGCCACTACGCA
CACGTTGACGCTCCAGGCCACGCCGACTACATCAAGAACATGATTACCGGCGCTGCTCAGATGGACGGCGCAATCCTCGT
TGTTGCTGCTACCGACGGCCCAATGCCTCAGACCCGTGAGCACGTTCTTCTTGCTCGCCAGGTTGGCGTTCCTTACATCC
TCGTTGCTCTTAACAAGTGCGACATGGTTGAGGATGAGGAAATCATCGAGCTCGTCGAGATGGAAGTTCGTGAACTTCTT
GCTGAGCAGGACTACGACGAAGAGGCTCCAATTGTTCACATCTCCGCTCTGAAGGCTCTTGAGGGCGACGAGAAGTGGGG
CAAGCAGATCCTTGAGCTCATGCAGGCTTGCGATGACAACATCCCTGACCCAGTTCGTGAGACCGACAAGCCATTCCTCA
TGCCTATCGAGGACATCTTCACCATCACCGGTCGTGGCACCGTTGTTACCGGTCGTGTTGAGCGCGGTACCCTGAACGTG
AACGATGATGTTGACATCATCGGCATCAAGGAGAAGTCCACCTCCACCACCGTTACCGGTATCGAGATGTTCCGTAAGCT
TCTTGACTCCGCTGAGGCTGGCGACAACTGTGGTCTGCTTCTCCGTGGTATCAAGCGCGAAGATGTTGAGCGTGGCCAGG
TTATCGTTAAGCCAGGCGCTTACACCCCTCACACCGAGTTCGAGGGCTCTGTCTACGTTCTGTCCAAGGATGAAGGTGGC
CGCCACACCCCATTCTTCGACAACTACCGTCCTCAGTTCTACTTCCGCACCACCGACGTTACCGGTGTTGTGAAGCTTCC
AGAGGGCACCGAGATGGTCATGCCTGGCGACAACGTCGACATGTCCGTCACCCTGATCCAGCCTGTCGCTATGGACGAGG
GCCTGCGTTTCGCTATCCGCGAAGGCTCCCGCACCGTTGGCGCTGGCCGTGTCACCAAGATCATCAAGTAA

Upstream 100 bases:

>100_bases
ATCTGTGTGCTCAGTCTTCCAGGCTGCTGATCACAGTGAAAGCAAAACCAATTCGTGGCTGCGAAAGTCGTAGCCACCAC
GAAGTCCAGGAGGACATACA

Downstream 100 bases:

>100_bases
TTTGATGCTCTAACTGTTGAGGTCTTTTAGACTTTGACTAACTGACAGCGATCCCAGAGGAAATATCCTCTGGGATCGCT
GTTTTGTCGTTTCTAGGGTT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTTTAAITKVLADAYPELNEAFAFDSIDKAPEEKERGITINISHVEYQTEKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKCDMVEDEEIIELVEMEVRELL
AEQDYDEEAPIVHISALKALEGDEKWGKQILELMQACDDNIPDPVRETDKPFLMPIEDIFTITGRGTVVTGRVERGTLNV
NDDVDIIGIKEKSTSTTVTGIEMFRKLLDSAEAGDNCGLLLRGIKREDVERGQVIVKPGAYTPHTEFEGSVYVLSKDEGG
RHTPFFDNYRPQFYFRTTDVTGVVKLPEGTEMVMPGDNVDMSVTLIQPVAMDEGLRFAIREGSRTVGAGRVTKIIK

Sequences:

>Translated_396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTTTAAITKVLADAYPELNEAFAFDSIDKAPEEKERGITINISHVEYQTEKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKCDMVEDEEIIELVEMEVRELL
AEQDYDEEAPIVHISALKALEGDEKWGKQILELMQACDDNIPDPVRETDKPFLMPIEDIFTITGRGTVVTGRVERGTLNV
NDDVDIIGIKEKSTSTTVTGIEMFRKLLDSAEAGDNCGLLLRGIKREDVERGQVIVKPGAYTPHTEFEGSVYVLSKDEGG
RHTPFFDNYRPQFYFRTTDVTGVVKLPEGTEMVMPGDNVDMSVTLIQPVAMDEGLRFAIREGSRTVGAGRVTKIIK
>Mature_395_residues
AKAKFERTKPHVNIGTIGHVDHGKTTTTAAITKVLADAYPELNEAFAFDSIDKAPEEKERGITINISHVEYQTEKRHYAH
VDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKCDMVEDEEIIELVEMEVRELLA
EQDYDEEAPIVHISALKALEGDEKWGKQILELMQACDDNIPDPVRETDKPFLMPIEDIFTITGRGTVVTGRVERGTLNVN
DDVDIIGIKEKSTSTTVTGIEMFRKLLDSAEAGDNCGLLLRGIKREDVERGQVIVKPGAYTPHTEFEGSVYVLSKDEGGR
HTPFFDNYRPQFYFRTTDVTGVVKLPEGTEMVMPGDNVDMSVTLIQPVAMDEGLRFAIREGSRTVGAGRVTKIIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=398, Percent_Identity=54.7738693467337, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI4503471, Length=455, Percent_Identity=30.7692307692308, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI4503475, Length=445, Percent_Identity=30.3370786516854, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI223555963, Length=445, Percent_Identity=28.5393258426966, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI5729864, Length=445, Percent_Identity=28.5393258426966, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI54607086, Length=323, Percent_Identity=28.1733746130031, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI194018522, Length=323, Percent_Identity=29.4117647058824, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI194018520, Length=323, Percent_Identity=29.4117647058824, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI194097354, Length=323, Percent_Identity=29.4117647058824, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI46094014, Length=333, Percent_Identity=29.7297297297297, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI4503507, Length=372, Percent_Identity=27.9569892473118, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI53729339, Length=232, Percent_Identity=31.4655172413793, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI53729337, Length=232, Percent_Identity=31.4655172413793, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=72.979797979798, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=72.979797979798, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI2367247, Length=384, Percent_Identity=30.2083333333333, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI48994988, Length=281, Percent_Identity=33.8078291814947, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789108, Length=263, Percent_Identity=28.5171102661597, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788922, Length=330, Percent_Identity=26.0606060606061, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=405, Percent_Identity=48.1481481481481, Blast_Score=353, Evalue=6e-98,
Organism=Caenorhabditis elegans, GI25141371, Length=375, Percent_Identity=43.4666666666667, Blast_Score=305, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI17552884, Length=449, Percent_Identity=29.8440979955457, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=449, Percent_Identity=29.8440979955457, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=436, Percent_Identity=27.7522935779816, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=326, Percent_Identity=31.2883435582822, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=326, Percent_Identity=31.2883435582822, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=274, Percent_Identity=32.1167883211679, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=341, Percent_Identity=26.6862170087977, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=42.0689655172414, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=28.1385281385281, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17509919, Length=311, Percent_Identity=26.3665594855305, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=400, Percent_Identity=62, Blast_Score=486, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6325337, Length=446, Percent_Identity=30.0448430493274, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=446, Percent_Identity=30.0448430493274, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=337, Percent_Identity=25.8160237388724, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=324, Percent_Identity=28.0864197530864, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=246, Percent_Identity=29.6747967479675, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281363316, Length=396, Percent_Identity=56.5656565656566, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI17864358, Length=396, Percent_Identity=56.5656565656566, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=49.6143958868895, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24652838, Length=442, Percent_Identity=29.4117647058824, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI17137572, Length=442, Percent_Identity=29.4117647058824, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI45553807, Length=444, Percent_Identity=30.8558558558559, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI45553816, Length=444, Percent_Identity=30.8558558558559, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24651721, Length=444, Percent_Identity=30.8558558558559, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI17864154, Length=444, Percent_Identity=30.8558558558559, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=31.4754098360656, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI45550900, Length=340, Percent_Identity=30, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI17137380, Length=323, Percent_Identity=28.7925696594427, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24647048, Length=372, Percent_Identity=26.3440860215054, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24647046, Length=372, Percent_Identity=26.3440860215054, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI78706572, Length=316, Percent_Identity=27.2151898734177, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28572034, Length=166, Percent_Identity=36.7469879518072, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43822; Mature: 43691

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTTTTAAITKVLADAYPELNEAFAFDSIDKAPEEKE
CCCCCCCCCCCCEEECEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHH
RGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKCDMVEDEEIIELVEMEVRELLAEQDYDEEAPIVHISALKAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHH
EGDEKWGKQILELMQACDDNIPDPVRETDKPFLMPIEDIFTITGRGTVVTGRVERGTLNV
CCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEHHHHEEEECCCEEEEEEEECCEEEC
NDDVDIIGIKEKSTSTTVTGIEMFRKLLDSAEAGDNCGLLLRGIKREDVERGQVIVKPGA
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCEEEECCCC
YTPHTEFEGSVYVLSKDEGGRHTPFFDNYRPQFYFRTTDVTGVVKLPEGTEMVMPGDNVD
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCC
MSVTLIQPVAMDEGLRFAIREGSRTVGAGRVTKIIK
EEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHCC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTTTTAAITKVLADAYPELNEAFAFDSIDKAPEEKE
CCCCCCCCCCCEEECEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHH
RGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKCDMVEDEEIIELVEMEVRELLAEQDYDEEAPIVHISALKAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHH
EGDEKWGKQILELMQACDDNIPDPVRETDKPFLMPIEDIFTITGRGTVVTGRVERGTLNV
CCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEHHHHEEEECCCEEEEEEEECCEEEC
NDDVDIIGIKEKSTSTTVTGIEMFRKLLDSAEAGDNCGLLLRGIKREDVERGQVIVKPGA
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCEEEECCCC
YTPHTEFEGSVYVLSKDEGGRHTPFFDNYRPQFYFRTTDVTGVVKLPEGTEMVMPGDNVD
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCC
MSVTLIQPVAMDEGLRFAIREGSRTVGAGRVTKIIK
EEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]