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The map label for this gene is menD

Identifier: 145294619

GI number: 145294619

Start: 627489

End: 629120

Strand: Direct

Name: menD

Synonym: cgR_0570

Alternate gene names: 145294619

Gene position: 627489-629120 (Clockwise)

Preceding gene: 145294618

Following gene: 145294620

Centisome position: 18.93

GC content: 57.78

Gene sequence:

>1632_bases
ATGTCCAGCACGCCAGCTCAAGATCTTGCCCGCGCCGTTATTGATTCCCTCGCACCACACGTCACTGACGTGGTGTTATG
CCCAGGATCCAGGAACTCACCGTTGTCGCTTGAGTTGCTGGCGCGGCAGGATCTGCGTGTCCATGTGCGTATCGACGAGC
GCAGCGCCTCATTTTTGGCGCTGTCCCTAGCGCGTACCCAGGCCCGGCCGGTGGCTGTGGTGATGACCTCCGGCACGGCT
GTGGCTAACTGCCTGCCTGCTGTTGCTGAAGCTGCGCATGCCCATATCCCGTTGATTGTGCTCTCTGCTGACCGTCCTGC
ACATTTGGTGGGAACGGGGGCGAGCCAAACGATTAACCAGACCGGTATTTTTGGTGATCTTGCACCGACGGTCGGTATCA
CTGAGCTGGATCAGGTAGCGCAGATTGCTGAAAGCCTTGCTCAGAGGGCTTCCCAGATTCCGCGTCATTTCAATCTTGCA
CTTGATGTTCCTTTGGTTGCTCCTGAACTGCCAGAGCTTCATGGTGAGGCAGTTGGAGCATCATGGACCCATCGCTGGAT
CAACCACGGTGAGGTGACCGTGGACCTAGGGGAGCACACCCTCGTGATTGCCGGTGATGAAGCATGGGAGGTTGAAGGGC
TGGAAGATGTGCCCACCATCGCTGAACCTACTGCACCAAAGCCTTATAATCCGGTGCACCCACTGGCTGCTGAAATATTG
CTGAAGGAGCAGGTCTCCGCGGAAGGCTATGTGGTGAACACCAGGCCTGATCATGTGATCGTGGTGGGCCACCCCACGCT
GCACCGCGGAGTGTTGAAGTTGATGTCAGATCCTGAGATCAAACTAACTGTGCTTTCACGCACCGATATCATCACTGATC
CCGGCCGCCATGCCGATCAGGTGGGCAGCACGGTGAAGGTCACCGGCACCCAAGAAAAGCAGTGGCTAAAGATCTGTTCG
GCAGCATCAGAACTTTCAGCCGATGGTGTGCGTGACGTCCTAGACAACCAAGAGTTCGGTTTCACCGGCCTGCACGTTGC
CGCAGCCGTGGCAGATACCTTAGGCACCGGCGATACTCTCTTCGCGACAGCATCCAACCCCATCCGCGACCTTTCCCTGG
TGGGTATGCCTTTTGACGGCGTGGATACCTTCTCCCCACGAGGTGTCGCAGGCATTGATGGTTCCGTAGCTCAAGCAATC
GGCACCGCACTTGCCGTGCAGTCCCGCCACCCCGATGAAATCCGCGCGCCACGCACTGTGGCCCTTCTGGGCGATCTGTC
GTTCCTTCACGATATTGGCGGACTGCTCATCGGCCCTGATGAACCACGCCCAGAAAACCTCACCATCGTGGTCGCCAACG
ACAACGGTGGTGGAATCTTCGAGCTCCTAGAAACCGGCGCAGACGGCCTCCGACCCAACTTCGAGCGTGCTTTCGGCACC
CCACACGACGCGTCCATCGCTGATCTCTGCGCAGGCTACGGCATTGAACACCAAGCGGTAGACAATCTCCAAGACCTCAT
CATCGCGCTAGTTGATACCACCGAAGTATCCGGATTCACCGTCATTGAAGCTTCGACCGTCCGCGATACCCGACGAGCGC
AACAACAAGCTCTCATGACCAAGGTGCGCTAA

Upstream 100 bases:

>100_bases
CGTTTGGAAACGCTTGCTGCCAGCAAAGATAGGCGTGATTGGTGGTTTGCGCGCGTGCGTGAATCGTATCCGTACCTGGA
GACAATCTAGACTGTTGTGC

Downstream 100 bases:

>100_bases
ATGGAGTGGTACCAAGTGCGCCGACGGACCCGACAACTGCTCATTGTCATCTATATAGCAGCCATGCTCGGCGCAGCTTC
TATGGTAATCGGGCCATTCC

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MSSTPAQDLARAVIDSLAPHVTDVVLCPGSRNSPLSLELLARQDLRVHVRIDERSASFLALSLARTQARPVAVVMTSGTA
VANCLPAVAEAAHAHIPLIVLSADRPAHLVGTGASQTINQTGIFGDLAPTVGITELDQVAQIAESLAQRASQIPRHFNLA
LDVPLVAPELPELHGEAVGASWTHRWINHGEVTVDLGEHTLVIAGDEAWEVEGLEDVPTIAEPTAPKPYNPVHPLAAEIL
LKEQVSAEGYVVNTRPDHVIVVGHPTLHRGVLKLMSDPEIKLTVLSRTDIITDPGRHADQVGSTVKVTGTQEKQWLKICS
AASELSADGVRDVLDNQEFGFTGLHVAAAVADTLGTGDTLFATASNPIRDLSLVGMPFDGVDTFSPRGVAGIDGSVAQAI
GTALAVQSRHPDEIRAPRTVALLGDLSFLHDIGGLLIGPDEPRPENLTIVVANDNGGGIFELLETGADGLRPNFERAFGT
PHDASIADLCAGYGIEHQAVDNLQDLIIALVDTTEVSGFTVIEASTVRDTRRAQQQALMTKVR

Sequences:

>Translated_543_residues
MSSTPAQDLARAVIDSLAPHVTDVVLCPGSRNSPLSLELLARQDLRVHVRIDERSASFLALSLARTQARPVAVVMTSGTA
VANCLPAVAEAAHAHIPLIVLSADRPAHLVGTGASQTINQTGIFGDLAPTVGITELDQVAQIAESLAQRASQIPRHFNLA
LDVPLVAPELPELHGEAVGASWTHRWINHGEVTVDLGEHTLVIAGDEAWEVEGLEDVPTIAEPTAPKPYNPVHPLAAEIL
LKEQVSAEGYVVNTRPDHVIVVGHPTLHRGVLKLMSDPEIKLTVLSRTDIITDPGRHADQVGSTVKVTGTQEKQWLKICS
AASELSADGVRDVLDNQEFGFTGLHVAAAVADTLGTGDTLFATASNPIRDLSLVGMPFDGVDTFSPRGVAGIDGSVAQAI
GTALAVQSRHPDEIRAPRTVALLGDLSFLHDIGGLLIGPDEPRPENLTIVVANDNGGGIFELLETGADGLRPNFERAFGT
PHDASIADLCAGYGIEHQAVDNLQDLIIALVDTTEVSGFTVIEASTVRDTRRAQQQALMTKVR
>Mature_542_residues
SSTPAQDLARAVIDSLAPHVTDVVLCPGSRNSPLSLELLARQDLRVHVRIDERSASFLALSLARTQARPVAVVMTSGTAV
ANCLPAVAEAAHAHIPLIVLSADRPAHLVGTGASQTINQTGIFGDLAPTVGITELDQVAQIAESLAQRASQIPRHFNLAL
DVPLVAPELPELHGEAVGASWTHRWINHGEVTVDLGEHTLVIAGDEAWEVEGLEDVPTIAEPTAPKPYNPVHPLAAEILL
KEQVSAEGYVVNTRPDHVIVVGHPTLHRGVLKLMSDPEIKLTVLSRTDIITDPGRHADQVGSTVKVTGTQEKQWLKICSA
ASELSADGVRDVLDNQEFGFTGLHVAAAVADTLGTGDTLFATASNPIRDLSLVGMPFDGVDTFSPRGVAGIDGSVAQAIG
TALAVQSRHPDEIRAPRTVALLGDLSFLHDIGGLLIGPDEPRPENLTIVVANDNGGGIFELLETGADGLRPNFERAFGTP
HDASIADLCAGYGIEHQAVDNLQDLIIALVDTTEVSGFTVIEASTVRDTRRAQQQALMTKVR

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=116, Percent_Identity=43.9655172413793, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_CORGB (A4QBE1)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001137440.1
- ProteinModelPortal:   A4QBE1
- STRING:   A4QBE1
- GeneID:   4993724
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_0570
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   IAEPTAP
- ProtClustDB:   PRK07449
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 57473; Mature: 57341

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTPAQDLARAVIDSLAPHVTDVVLCPGSRNSPLSLELLARQDLRVHVRIDERSASFLA
CCCCCHHHHHHHHHHHHCCHHEEEEECCCCCCCCEEEEEEECCCCEEEEEECCCCCCEEE
LSLARTQARPVAVVMTSGTAVANCLPAVAEAAHAHIPLIVLSADRPAHLVGTGASQTINQ
EEHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCHHHHC
TGIFGDLAPTVGITELDQVAQIAESLAQRASQIPRHFNLALDVPLVAPELPELHGEAVGA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHCCCCCCC
SWTHRWINHGEVTVDLGEHTLVIAGDEAWEVEGLEDVPTIAEPTAPKPYNPVHPLAAEIL
CHHHHHCCCCEEEEEECCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LKEQVSAEGYVVNTRPDHVIVVGHPTLHRGVLKLMSDPEIKLTVLSRTDIITDPGRHADQ
HHHHCCCCCEEEECCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCEECCCCCCHHH
VGSTVKVTGTQEKQWLKICSAASELSADGVRDVLDNQEFGFTGLHVAAAVADTLGTGDTL
CCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEE
FATASNPIRDLSLVGMPFDGVDTFSPRGVAGIDGSVAQAIGTALAVQSRHPDEIRAPRTV
EEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCEEE
ALLGDLSFLHDIGGLLIGPDEPRPENLTIVVANDNGGGIFELLETGADGLRPNFERAFGT
EEHHHHHHHHHCCCEEECCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCHHHHCCC
PHDASIADLCAGYGIEHQAVDNLQDLIIALVDTTEVSGFTVIEASTVRDTRRAQQQALMT
CCCCCHHHHHHCCCCCHHHHHHHHHHHHEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHH
KVR
HCC
>Mature Secondary Structure 
SSTPAQDLARAVIDSLAPHVTDVVLCPGSRNSPLSLELLARQDLRVHVRIDERSASFLA
CCCCHHHHHHHHHHHHCCHHEEEEECCCCCCCCEEEEEEECCCCEEEEEECCCCCCEEE
LSLARTQARPVAVVMTSGTAVANCLPAVAEAAHAHIPLIVLSADRPAHLVGTGASQTINQ
EEHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCHHHHC
TGIFGDLAPTVGITELDQVAQIAESLAQRASQIPRHFNLALDVPLVAPELPELHGEAVGA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHCCCCCCC
SWTHRWINHGEVTVDLGEHTLVIAGDEAWEVEGLEDVPTIAEPTAPKPYNPVHPLAAEIL
CHHHHHCCCCEEEEEECCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LKEQVSAEGYVVNTRPDHVIVVGHPTLHRGVLKLMSDPEIKLTVLSRTDIITDPGRHADQ
HHHHCCCCCEEEECCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCEECCCCCCHHH
VGSTVKVTGTQEKQWLKICSAASELSADGVRDVLDNQEFGFTGLHVAAAVADTLGTGDTL
CCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEE
FATASNPIRDLSLVGMPFDGVDTFSPRGVAGIDGSVAQAIGTALAVQSRHPDEIRAPRTV
EEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCEEE
ALLGDLSFLHDIGGLLIGPDEPRPENLTIVVANDNGGGIFELLETGADGLRPNFERAFGT
EEHHHHHHHHHCCCEEECCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCHHHHCCC
PHDASIADLCAGYGIEHQAVDNLQDLIIALVDTTEVSGFTVIEASTVRDTRRAQQQALMT
CCCCCHHHHHHCCCCCHHHHHHHHHHHHEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHH
KVR
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA