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The map label for this gene is mshA

Identifier: 145294518

GI number: 145294518

Start: 528971

End: 530227

Strand: Direct

Name: mshA

Synonym: cgR_0473

Alternate gene names: 145294518

Gene position: 528971-530227 (Clockwise)

Preceding gene: 145294516

Following gene: 145294519

Centisome position: 15.96

GC content: 57.76

Gene sequence:

>1257_bases
ATGCGCGTAGCTATGATTTCCATGCACACCTCTCCATTGCAGCAGCCCGGAACTGGTGATTCAGGCGGCATGAACGTCTA
CATTCTTTCGACCGCGACTGAGCTAGCGAAACAGGGTATCGAGGTCGATATTTACACTCGTGCCACGAGGCCTTCTCAGG
GCGAGATCGTGAGAGTTGCTGAGAATTTGCGGGTCATTAATATCGCTGCGGGGCCGTATGAGGGGCTTTCCAAAGAGGAA
CTTCCTACCCAATTGGCGGCGTTTACCGGCGGAATGTTGTCGTTTACGCGCCGGGAGAAGGTTACTTATGATCTGATCCA
TTCTCACTATTGGCTTTCTGGGCAGGTGGGGTGGTTGCTGCGCGATTTGTGGCGGATTCCCCTTATTCATACGGCACACA
CTTTGGCGGCGGTGAAGAATTCTTATCGGGATGATTCGGATACCCCGGAGTCGGAGGCGCGTCGCATTTGTGAGCAGCAG
CTGGTGGATAACGCTGACGTGTTGGCGGTGAACACTCAGGAGGAGATGCAGGATTTGATGCATCACTACGATGCGGATCC
GGATCGGATTTCTGTGGTGTCGCCGGGTGCAGATGTGGAACTTTATAGCCCTGGAAATGATCGCGCGACGGAACGTTCCC
GTCGTGAGCTGGGCATTCCGCTGCACACAAAGGTGGTGGCTTTTGTGGGTCGGTTGCAGCCGTTTAAGGGCCCGCAGGTG
CTGATCAAGGCGGTTGCGGCGTTGTTTGATCGCGATCCGGACCGAAATCTGCGCGTCATTATTTGTGGCGGCCCTTCTGG
TCCGAATGCGACACCGGATACCTATAGGCATATGGCAGAGGAACTGGGCGTCGAAAAGCGAATTCGCTTTTTGGACCCGC
GCCCGCCGAGCGAGCTAGTGGCCGTGTATCGGGCGGCGGACATCGTGGCCGTGCCCAGTTTTAATGAGTCCTTCGGACTC
GTCGCCATGGAGGCGCAAGCCAGCGGCACACCGGTCATTGCGGCCCGGGTTGGCGGCCTGCCCATCGCAGTCGCGGAAGG
GGAGACGGGATTGCTTGTCGACGGCCACTCCCCGCATGCCTGGGCCGACGCCTTAGCCACACTCTTGGACGATGACGAAA
CGCGCATCAGGATGGGCGAAGATGCCGTCGAACACGCCAGAACATTCTCCTGGGCAGCCACCGCCGCGCAGCTATCGTCG
CTGTACAACGACGCTATTGCCAACGAAAACGTCGACGGTGAAACGCATCACGGCTAA

Upstream 100 bases:

>100_bases
TTTCGTATGCTGACATGGTGTCCCTTCAACTGCGTTGCTTTAGTGCCCTTTAGTATATAGAGACGTCCCGCTGCTTTCTT
CGGCGATCTAGAATGTGGGC

Downstream 100 bases:

>100_bases
GTAAACGCGCGTCTTGGAACATAAAGTGGCAAACTAGTACCTATGACTAACGGAAAATTGATTCTTCTTCGTCACGGTCA
GAGCGAATGGAACGCATCCA

Product: hypothetical protein

Products: NA

Alternate protein names: N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferase; GlcNAc-Ins-P N-acetylglucosaminyltransferase

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MRVAMISMHTSPLQQPGTGDSGGMNVYILSTATELAKQGIEVDIYTRATRPSQGEIVRVAENLRVINIAAGPYEGLSKEE
LPTQLAAFTGGMLSFTRREKVTYDLIHSHYWLSGQVGWLLRDLWRIPLIHTAHTLAAVKNSYRDDSDTPESEARRICEQQ
LVDNADVLAVNTQEEMQDLMHHYDADPDRISVVSPGADVELYSPGNDRATERSRRELGIPLHTKVVAFVGRLQPFKGPQV
LIKAVAALFDRDPDRNLRVIICGGPSGPNATPDTYRHMAEELGVEKRIRFLDPRPPSELVAVYRAADIVAVPSFNESFGL
VAMEAQASGTPVIAARVGGLPIAVAEGETGLLVDGHSPHAWADALATLLDDDETRIRMGEDAVEHARTFSWAATAAQLSS
LYNDAIANENVDGETHHG

Sequences:

>Translated_418_residues
MRVAMISMHTSPLQQPGTGDSGGMNVYILSTATELAKQGIEVDIYTRATRPSQGEIVRVAENLRVINIAAGPYEGLSKEE
LPTQLAAFTGGMLSFTRREKVTYDLIHSHYWLSGQVGWLLRDLWRIPLIHTAHTLAAVKNSYRDDSDTPESEARRICEQQ
LVDNADVLAVNTQEEMQDLMHHYDADPDRISVVSPGADVELYSPGNDRATERSRRELGIPLHTKVVAFVGRLQPFKGPQV
LIKAVAALFDRDPDRNLRVIICGGPSGPNATPDTYRHMAEELGVEKRIRFLDPRPPSELVAVYRAADIVAVPSFNESFGL
VAMEAQASGTPVIAARVGGLPIAVAEGETGLLVDGHSPHAWADALATLLDDDETRIRMGEDAVEHARTFSWAATAAQLSS
LYNDAIANENVDGETHHG
>Mature_418_residues
MRVAMISMHTSPLQQPGTGDSGGMNVYILSTATELAKQGIEVDIYTRATRPSQGEIVRVAENLRVINIAAGPYEGLSKEE
LPTQLAAFTGGMLSFTRREKVTYDLIHSHYWLSGQVGWLLRDLWRIPLIHTAHTLAAVKNSYRDDSDTPESEARRICEQQ
LVDNADVLAVNTQEEMQDLMHHYDADPDRISVVSPGADVELYSPGNDRATERSRRELGIPLHTKVVAFVGRLQPFKGPQV
LIKAVAALFDRDPDRNLRVIICGGPSGPNATPDTYRHMAEELGVEKRIRFLDPRPPSELVAVYRAADIVAVPSFNESFGL
VAMEAQASGTPVIAARVGGLPIAVAEGETGLLVDGHSPHAWADALATLLDDDETRIRMGEDAVEHARTFSWAATAAQLSS
LYNDAIANENVDGETHHG

Specific function: Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2- acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family. MshA subfamily

Homologues:

Organism=Escherichia coli, GI1790061, Length=224, Percent_Identity=27.2321428571429, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSHA_CORGB (A4QB40)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001137339.1
- ProteinModelPortal:   A4QB40
- SMR:   A4QB40
- STRING:   A4QB40
- GeneID:   4993081
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_0473
- eggNOG:   COG0438
- HOGENOM:   HBG726846
- OMA:   DETRIRM
- ProtClustDB:   CLSK2303180
- HAMAP:   MF_01695
- InterPro:   IPR001296
- InterPro:   IPR017814
- InterPro:   IPR013534
- TIGRFAMs:   TIGR03449

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1

EC number: =2.4.1.250

Molecular weight: Translated: 45670; Mature: 45670

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: BINDING 9-9 BINDING 23-23 BINDING 78-78 BINDING 110-110 BINDING 134-134 BINDING 154-154 BINDING 231-231 BINDING 236-236 BINDING 294-294 BINDING 316-316 BINDING 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAMISMHTSPLQQPGTGDSGGMNVYILSTATELAKQGIEVDIYTRATRPSQGEIVRVA
CEEEEEEECCCCCCCCCCCCCCCEEEEEEECHHHHHHCCCEEEEEEECCCCCCCCEEEEE
ENLRVINIAAGPYEGLSKEELPTQLAAFTGGMLSFTRREKVTYDLIHSHYWLSGQVGWLL
CCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCEEEECCCHHHHH
RDLWRIPLIHTAHTLAAVKNSYRDDSDTPESEARRICEQQLVDNADVLAVNTQEEMQDLM
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHH
HHYDADPDRISVVSPGADVELYSPGNDRATERSRRELGIPLHTKVVAFVGRLQPFKGPQV
HHHCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHH
LIKAVAALFDRDPDRNLRVIICGGPSGPNATPDTYRHMAEELGVEKRIRFLDPRPPSELV
HHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCHHHHH
AVYRAADIVAVPSFNESFGLVAMEAQASGTPVIAARVGGLPIAVAEGETGLLVDGHSPHA
HHHHHHCEEEECCCCCCCCEEEEECCCCCCCEEEEECCCEEEEEECCCCEEEEECCCCHH
WADALATLLDDDETRIRMGEDAVEHARTFSWAATAAQLSSLYNDAIANENVDGETHHG
HHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRVAMISMHTSPLQQPGTGDSGGMNVYILSTATELAKQGIEVDIYTRATRPSQGEIVRVA
CEEEEEEECCCCCCCCCCCCCCCEEEEEEECHHHHHHCCCEEEEEEECCCCCCCCEEEEE
ENLRVINIAAGPYEGLSKEELPTQLAAFTGGMLSFTRREKVTYDLIHSHYWLSGQVGWLL
CCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCEEEECCCHHHHH
RDLWRIPLIHTAHTLAAVKNSYRDDSDTPESEARRICEQQLVDNADVLAVNTQEEMQDLM
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHH
HHYDADPDRISVVSPGADVELYSPGNDRATERSRRELGIPLHTKVVAFVGRLQPFKGPQV
HHHCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHH
LIKAVAALFDRDPDRNLRVIICGGPSGPNATPDTYRHMAEELGVEKRIRFLDPRPPSELV
HHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCHHHHH
AVYRAADIVAVPSFNESFGLVAMEAQASGTPVIAARVGGLPIAVAEGETGLLVDGHSPHA
HHHHHHCEEEECCCCCCCCEEEEECCCCCCCEEEEECCCEEEEEECCCCEEEEECCCCHH
WADALATLLDDDETRIRMGEDAVEHARTFSWAATAAQLSSLYNDAIANENVDGETHHG
HHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA