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The map label for this gene is yfiY [H]

Identifier: 145294492

GI number: 145294492

Start: 500065

End: 500946

Strand: Direct

Name: yfiY [H]

Synonym: cgR_0447

Alternate gene names: 145294492

Gene position: 500065-500946 (Clockwise)

Preceding gene: 145294491

Following gene: 145294495

Centisome position: 15.09

GC content: 57.26

Gene sequence:

>882_bases
ATGAAAAAGTATGCAGTCGCGGCCCTCGCGCTACTCACCCTGGCGGGATGTTCTACTAGTGAAGAGGTGGCGGTTTCTTC
TGACTCGGCTCCGCAGAGGATTGTGATTGCCCAAGCCAACTTCCTCGATCTCGCCCTCGCACTCGACCTCGAACCAGTCG
GAACCACCTACTGGGGCGGAGCCGGAGGAGTGCAGGATTATTTGCTAGATTCCGTCCCTGCATCGATGGAAGTAGTTGGA
AACGACGACGAACCCAACTTCGAAACCGTCGCCAAACTCCAACCCGACCTCATCATCGGCGACGAAGAAATCGAAACCAA
CCTCGCCAAATACGAAGCCATCGCACCCGTCTCCATCATCAAATCCGTCGACGATAACGGATCCAACAGCTGGCGCGACC
AACTCACCGCCCTCGCTGAACTCACCGGCACCCAAGAAAAAGCAGCCGCAGTGATTGAAGAAGCGGACAAATCCGTAGCT
TCACTGGACTCGAAGATTACGTCGCCCGGTGAGAAGACCATGCTGCTCCGCATCCGCGAAGAACAAGTCCGCCAATACTT
CCCCGACAGCTTCGTCGGCGCCGGCGTCCCCCAACGCCTCCAAAACATCACCCTCGTCGAATCCGCCGTCCCCTCCGAAA
ACGGCCAATGGTCAGTCATTCCACCCGAAAACATCGGACTTCTCGACGCCGACCGCATCATCGCCTTCATCGACAGCCCC
CAAGCCCTCGAAAACGCCGAAAGCAACCCACTGTGGAGACAACTCCCCGCAGTCAAAAACGGTCAACTCTGTACCACGGA
AAACCTCACCCCATGGATCCTCACCGGACCAGCAGCAGCCGAGATCGTGACCTCTGACCTCGAGGCATGTTTCGCTGCTT
AG

Upstream 100 bases:

>100_bases
TGTTGTTTTGTTTTTGCTTGGAGTTCCCTGGATTTGTATAGTTTAGCCTGCCCTAATGTATCTACCGTGGGTAGATAATT
TGATCGAATTGGAAGGCTAT

Downstream 100 bases:

>100_bases
GTGGGGTGTCGAAACCCTGCAATTTCTGGGAAAAATTCGCAGTTCATGATGCCGCTGGGCTGTGTCTAGCCGGGTGGTTT
CTCCACACTGTCGAATTGAG

Product: hypothetical protein

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKKYAVAALALLTLAGCSTSEEVAVSSDSAPQRIVIAQANFLDLALALDLEPVGTTYWGGAGGVQDYLLDSVPASMEVVG
NDDEPNFETVAKLQPDLIIGDEEIETNLAKYEAIAPVSIIKSVDDNGSNSWRDQLTALAELTGTQEKAAAVIEEADKSVA
SLDSKITSPGEKTMLLRIREEQVRQYFPDSFVGAGVPQRLQNITLVESAVPSENGQWSVIPPENIGLLDADRIIAFIDSP
QALENAESNPLWRQLPAVKNGQLCTTENLTPWILTGPAAAEIVTSDLEACFAA

Sequences:

>Translated_293_residues
MKKYAVAALALLTLAGCSTSEEVAVSSDSAPQRIVIAQANFLDLALALDLEPVGTTYWGGAGGVQDYLLDSVPASMEVVG
NDDEPNFETVAKLQPDLIIGDEEIETNLAKYEAIAPVSIIKSVDDNGSNSWRDQLTALAELTGTQEKAAAVIEEADKSVA
SLDSKITSPGEKTMLLRIREEQVRQYFPDSFVGAGVPQRLQNITLVESAVPSENGQWSVIPPENIGLLDADRIIAFIDSP
QALENAESNPLWRQLPAVKNGQLCTTENLTPWILTGPAAAEIVTSDLEACFAA
>Mature_293_residues
MKKYAVAALALLTLAGCSTSEEVAVSSDSAPQRIVIAQANFLDLALALDLEPVGTTYWGGAGGVQDYLLDSVPASMEVVG
NDDEPNFETVAKLQPDLIIGDEEIETNLAKYEAIAPVSIIKSVDDNGSNSWRDQLTALAELTGTQEKAAAVIEEADKSVA
SLDSKITSPGEKTMLLRIREEQVRQYFPDSFVGAGVPQRLQNITLVESAVPSENGQWSVIPPENIGLLDADRIIAFIDSP
QALENAESNPLWRQLPAVKNGQLCTTENLTPWILTGPAAAEIVTSDLEACFAA

Specific function: Part of the ABC transporter complex yfiYZ/yfhA/yusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen. Binds the siderophores and delivers them to the surface of yfiZ/yfhA (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable). Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082411, Length=299, Percent_Identity=23.0769230769231, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 31298; Mature: 31298

Theoretical pI: Translated: 3.87; Mature: 3.87

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYAVAALALLTLAGCSTSEEVAVSSDSAPQRIVIAQANFLDLALALDLEPVGTTYWGG
CCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCC
AGGVQDYLLDSVPASMEVVGNDDEPNFETVAKLQPDLIIGDEEIETNLAKYEAIAPVSII
CCCHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHH
KSVDDNGSNSWRDQLTALAELTGTQEKAAAVIEEADKSVASLDSKITSPGEKTMLLRIRE
HHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHH
EQVRQYFPDSFVGAGVPQRLQNITLVESAVPSENGQWSVIPPENIGLLDADRIIAFIDSP
HHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEECHHHEEEEECCC
QALENAESNPLWRQLPAVKNGQLCTTENLTPWILTGPAAAEIVTSDLEACFAA
HHHHCCCCCCHHHHCCCCCCCCEEEECCCCCEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKYAVAALALLTLAGCSTSEEVAVSSDSAPQRIVIAQANFLDLALALDLEPVGTTYWGG
CCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCC
AGGVQDYLLDSVPASMEVVGNDDEPNFETVAKLQPDLIIGDEEIETNLAKYEAIAPVSII
CCCHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHH
KSVDDNGSNSWRDQLTALAELTGTQEKAAAVIEEADKSVASLDSKITSPGEKTMLLRIRE
HHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHH
EQVRQYFPDSFVGAGVPQRLQNITLVESAVPSENGQWSVIPPENIGLLDADRIIAFIDSP
HHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEECHHHEEEEECCC
QALENAESNPLWRQLPAVKNGQLCTTENLTPWILTGPAAAEIVTSDLEACFAA
HHHHCCCCCCHHHHCCCCCCCCEEEECCCCCEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]