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The map label for this gene is tgt [H]

Identifier: 145294351

GI number: 145294351

Start: 332820

End: 334085

Strand: Direct

Name: tgt [H]

Synonym: cgR_0306

Alternate gene names: 145294351

Gene position: 332820-334085 (Clockwise)

Preceding gene: 145294350

Following gene: 145294353

Centisome position: 10.04

GC content: 56.0

Gene sequence:

>1266_bases
TTGGCACCTATGTCAGATCTTTCATTCACCCTCAACAACAAGCTTGCCGATGACGCCCCCGGCAAGCATGGTCGTACAGG
TGTAATCCACACACCTCACGGTGATATTGCCACCCCTGCTTTCATTCCTGTGGCTACCAAAGCCACGGTGAAAACCCTTA
CCCCGGAGCAGATTCGGGAGACCGGCGCACAGGCTATTTTGTCCAACGCTTACCACCTGTATCTGCAGCCTGGCCCCGAC
ATCGTGGACGAGGCTGGCGGTGTGTCTGCCTTTGAGAACTGGCACGGCCCCACCTACACCGACTCCGGCGGATTCCAGGT
CATGAGCCTGGGCTCCGGCTTTAAAAAGGTGCTGGCCATGGACACCACCAACTTGACGCGCAACGATATCAAGGCGGCTA
AAAAAGAGCGCATGGCGCTTGTCGACGAAGACGGCGTGGATTTCAAATCTGTTATCGATGGCTCAAAGCACCGATTCACC
CCAGAAGTCTCCATGCAGATTCAGCACCAATTGGGCGCAGACATCATCTTCGCTTTTGATGAACTGACCACCTTGGTTGA
TACCTATGACTACCAGGTGGAATCTGTGGAACGTACCCGCAGGTGGGCACAGCGCTGCCTTCTAGAGCATGAACGTTTGA
CTCAGGAACGTGTCGGAAAACCACTTCAATCCCTATGGGGAGTGGTGCAGGGGGCGCAGTTCGAGGATTTGCGTCGACAA
GCAGTAAAAGGCCTGTTGGACCTGGATCGCCAGGCCGCCGACGAAGGCCGTCGTGGCTTCGGCGGTTTCGGCATCGGTGG
CGCGCTCGAGAAAGAAAACTTGGGCACCATCGTGGGCTGGGTATGCGATGAGCTGCCTGATGAGAAGCCTCGTCACCTGC
TGGGTATTTCCGAGCCGGACGATCTGTTCGTGGCCGTGGAAGCTGGCGCGGACACCTTCGACTGCGTGGCGCCGACCCGT
TTAGGCCGACGCGGCGGTGTCTACACACTTGATGGCCGTATGAACTTGACTGGTGCGCGCTTCAAGCGTGACTTCAAGGG
CATCGATGAGGAAGTCGGCGGATACGCCAGCGAGAACTACTCCCGCGCGTACATTCATCACCTGCTCAAAGCGAAGGAAT
TTTTGGCCGGCACGCTGTGCACCATGCACAATCTGTACTTCATGATCACCTTGGTGGATAAGATTCGTGCCAGCATCGAT
GATGGCACGTACTACGAATTCAAGGAAGAATTCTTAGGCCGCTACTACGCGTCGAAGGTTTCATAA

Upstream 100 bases:

>100_bases
GGTGGATGCCTAAGTGGCTGGATCGAATTCTGCCAAGTTTGGACATTGAAGGCACCGCCCTGGAGAAGGAATGGGAGGAG
AAGCAGGCTGCACGTTAGAC

Downstream 100 bases:

>100_bases
CCTTCGCGGCGTTTCACCCACCTAATGACTGCGTAGGTAATGGGCATGAGGATGACCTCTAGAAGGGTTTTCCACACGAA
GCCCACCACAACGTAGTTGA

Product: hypothetical protein

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MAPMSDLSFTLNNKLADDAPGKHGRTGVIHTPHGDIATPAFIPVATKATVKTLTPEQIRETGAQAILSNAYHLYLQPGPD
IVDEAGGVSAFENWHGPTYTDSGGFQVMSLGSGFKKVLAMDTTNLTRNDIKAAKKERMALVDEDGVDFKSVIDGSKHRFT
PEVSMQIQHQLGADIIFAFDELTTLVDTYDYQVESVERTRRWAQRCLLEHERLTQERVGKPLQSLWGVVQGAQFEDLRRQ
AVKGLLDLDRQAADEGRRGFGGFGIGGALEKENLGTIVGWVCDELPDEKPRHLLGISEPDDLFVAVEAGADTFDCVAPTR
LGRRGGVYTLDGRMNLTGARFKRDFKGIDEEVGGYASENYSRAYIHHLLKAKEFLAGTLCTMHNLYFMITLVDKIRASID
DGTYYEFKEEFLGRYYASKVS

Sequences:

>Translated_421_residues
MAPMSDLSFTLNNKLADDAPGKHGRTGVIHTPHGDIATPAFIPVATKATVKTLTPEQIRETGAQAILSNAYHLYLQPGPD
IVDEAGGVSAFENWHGPTYTDSGGFQVMSLGSGFKKVLAMDTTNLTRNDIKAAKKERMALVDEDGVDFKSVIDGSKHRFT
PEVSMQIQHQLGADIIFAFDELTTLVDTYDYQVESVERTRRWAQRCLLEHERLTQERVGKPLQSLWGVVQGAQFEDLRRQ
AVKGLLDLDRQAADEGRRGFGGFGIGGALEKENLGTIVGWVCDELPDEKPRHLLGISEPDDLFVAVEAGADTFDCVAPTR
LGRRGGVYTLDGRMNLTGARFKRDFKGIDEEVGGYASENYSRAYIHHLLKAKEFLAGTLCTMHNLYFMITLVDKIRASID
DGTYYEFKEEFLGRYYASKVS
>Mature_420_residues
APMSDLSFTLNNKLADDAPGKHGRTGVIHTPHGDIATPAFIPVATKATVKTLTPEQIRETGAQAILSNAYHLYLQPGPDI
VDEAGGVSAFENWHGPTYTDSGGFQVMSLGSGFKKVLAMDTTNLTRNDIKAAKKERMALVDEDGVDFKSVIDGSKHRFTP
EVSMQIQHQLGADIIFAFDELTTLVDTYDYQVESVERTRRWAQRCLLEHERLTQERVGKPLQSLWGVVQGAQFEDLRRQA
VKGLLDLDRQAADEGRRGFGGFGIGGALEKENLGTIVGWVCDELPDEKPRHLLGISEPDDLFVAVEAGADTFDCVAPTRL
GRRGGVYTLDGRMNLTGARFKRDFKGIDEEVGGYASENYSRAYIHHLLKAKEFLAGTLCTMHNLYFMITLVDKIRASIDD
GTYYEFKEEFLGRYYASKVS

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=403, Percent_Identity=33.4987593052109, Blast_Score=238, Evalue=6e-63,
Organism=Escherichia coli, GI1786607, Length=404, Percent_Identity=37.3762376237624, Blast_Score=269, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17544680, Length=394, Percent_Identity=32.994923857868, Blast_Score=227, Evalue=8e-60,
Organism=Drosophila melanogaster, GI19920510, Length=409, Percent_Identity=32.2738386308068, Blast_Score=234, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 46667; Mature: 46536

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPMSDLSFTLNNKLADDAPGKHGRTGVIHTPHGDIATPAFIPVATKATVKTLTPEQIRE
CCCCCCCCEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEECCHHHCCCCHHHHHH
TGAQAILSNAYHLYLQPGPDIVDEAGGVSAFENWHGPTYTDSGGFQVMSLGSGFKKVLAM
HHHHHHHCCCEEEEECCCCCHHHCCCCCCHHHCCCCCCCCCCCCEEEEECCCCHHHHHHH
DTTNLTRNDIKAAKKERMALVDEDGVDFKSVIDGSKHRFTPEVSMQIQHQLGADIIFAFD
CCCCCCHHHHHHHHHHHHEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEHHH
ELTTLVDTYDYQVESVERTRRWAQRCLLEHERLTQERVGKPLQSLWGVVQGAQFEDLRRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHH
AVKGLLDLDRQAADEGRRGFGGFGIGGALEKENLGTIVGWVCDELPDEKPRHLLGISEPD
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCCCC
DLFVAVEAGADTFDCVAPTRLGRRGGVYTLDGRMNLTGARFKRDFKGIDEEVGGYASENY
CEEEEEECCCCCCHHCCCHHCCCCCCEEEECCCCCCCHHHHHHHHCCCHHHHCCCCCCCC
SRAYIHHLLKAKEFLAGTLCTMHNLYFMITLVDKIRASIDDGTYYEFKEEFLGRYYASKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
S
C
>Mature Secondary Structure 
APMSDLSFTLNNKLADDAPGKHGRTGVIHTPHGDIATPAFIPVATKATVKTLTPEQIRE
CCCCCCCEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEECCHHHCCCCHHHHHH
TGAQAILSNAYHLYLQPGPDIVDEAGGVSAFENWHGPTYTDSGGFQVMSLGSGFKKVLAM
HHHHHHHCCCEEEEECCCCCHHHCCCCCCHHHCCCCCCCCCCCCEEEEECCCCHHHHHHH
DTTNLTRNDIKAAKKERMALVDEDGVDFKSVIDGSKHRFTPEVSMQIQHQLGADIIFAFD
CCCCCCHHHHHHHHHHHHEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEHHH
ELTTLVDTYDYQVESVERTRRWAQRCLLEHERLTQERVGKPLQSLWGVVQGAQFEDLRRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHH
AVKGLLDLDRQAADEGRRGFGGFGIGGALEKENLGTIVGWVCDELPDEKPRHLLGISEPD
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCCCC
DLFVAVEAGADTFDCVAPTRLGRRGGVYTLDGRMNLTGARFKRDFKGIDEEVGGYASENY
CEEEEEECCCCCCHHCCCHHCCCCCCEEEECCCCCCCHHHHHHHHCCCHHHHCCCCCCCC
SRAYIHHLLKAKEFLAGTLCTMHNLYFMITLVDKIRASIDDGTYYEFKEEFLGRYYASKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA