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The map label for this gene is alkB [H]

Identifier: 145294267

GI number: 145294267

Start: 241085

End: 241777

Strand: Direct

Name: alkB [H]

Synonym: cgR_0223

Alternate gene names: 145294267

Gene position: 241085-241777 (Clockwise)

Preceding gene: 145294263

Following gene: 145294268

Centisome position: 7.27

GC content: 53.25

Gene sequence:

>693_bases
GTGCCTACGCTTTTCGACGATCCCCTCCACCAACTTCCCAGACCACCGCGCAGGGTTGCAGCTGGCGTGGTGCACCTGCC
CAACTTCCTCGGCTTGGCCGAGCAAAAGGCACTGGTCGCCCAGGCTCGAGACCTTGCGCGTGAAGTTGTGGGCACACCGT
TAGCGATGGTCCGACCCAAGCTGAAAAGTGGTCAGATGAGTGTCCATATGTTGCATTTAGGAAAGTACTGGGCATCCAAC
CCTTATCGCTATGTGGATGTCGTTGATGGTTTTCCAGTGCCACCACTGCCGGATTCTTTCGTGGACTTGGCACACCGAGC
ATTGCTGTCTGCAGGATCTTTAAGCAATTCACTTCAGTCTTGGTCGGAGGCATATCGAGCAGAAGCGGCATTGGTGAATT
ACTACTCGCCAGATGCTTCAATGGGCATGCACCAAGACGCTAATGAAGAATCGGAAGCCCCCGTGATCTCCCTATCGATC
GGCGACACCGGAATCTTCCGATTGGGCGGCACTCTAAACCGCAATAAACCGTGGACAGATATCCCACTAATGAGTGGCGA
TCTCATCGTTTTTGGAGGTGCAAACAGGCGAGCATTTCATGGCATTCCCTTTATTGAGGCCAATACTGCACCTGCAGGAT
GCGGGTTGAAAGAAGGGCGAATCAACATCACGATCCGCCAACTTGCACTATAA

Upstream 100 bases:

>100_bases
TCACGTTCCGCGCCGGTCGCCACGTACCAATCGCCGCTTGTCATTTTCTCGATAGATTCAAAGTCCTTGTCCATATCCTC
CATTGTGCAGGAGAACTTTA

Downstream 100 bases:

>100_bases
AACTACAGGTAGAGGTATGTCTCGGGGGTGAAATAGCCCCGCAATGTCCCATGCTCAAATTATGGTTCGCACCATGACAA
CAAACAAAGCTAATCCAAAT

Product: hypothetical protein

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MPTLFDDPLHQLPRPPRRVAAGVVHLPNFLGLAEQKALVAQARDLAREVVGTPLAMVRPKLKSGQMSVHMLHLGKYWASN
PYRYVDVVDGFPVPPLPDSFVDLAHRALLSAGSLSNSLQSWSEAYRAEAALVNYYSPDASMGMHQDANEESEAPVISLSI
GDTGIFRLGGTLNRNKPWTDIPLMSGDLIVFGGANRRAFHGIPFIEANTAPAGCGLKEGRINITIRQLAL

Sequences:

>Translated_230_residues
MPTLFDDPLHQLPRPPRRVAAGVVHLPNFLGLAEQKALVAQARDLAREVVGTPLAMVRPKLKSGQMSVHMLHLGKYWASN
PYRYVDVVDGFPVPPLPDSFVDLAHRALLSAGSLSNSLQSWSEAYRAEAALVNYYSPDASMGMHQDANEESEAPVISLSI
GDTGIFRLGGTLNRNKPWTDIPLMSGDLIVFGGANRRAFHGIPFIEANTAPAGCGLKEGRINITIRQLAL
>Mature_229_residues
PTLFDDPLHQLPRPPRRVAAGVVHLPNFLGLAEQKALVAQARDLAREVVGTPLAMVRPKLKSGQMSVHMLHLGKYWASNP
YRYVDVVDGFPVPPLPDSFVDLAHRALLSAGSLSNSLQSWSEAYRAEAALVNYYSPDASMGMHQDANEESEAPVISLSIG
DTGIFRLGGTLNRNKPWTDIPLMSGDLIVFGGANRRAFHGIPFIEANTAPAGCGLKEGRINITIRQLAL

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=95, Percent_Identity=38.9473684210526, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1788541, Length=217, Percent_Identity=34.1013824884793, Blast_Score=89, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45555401, Length=93, Percent_Identity=38.7096774193548, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24988; Mature: 24857

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLFDDPLHQLPRPPRRVAAGVVHLPNFLGLAEQKALVAQARDLAREVVGTPLAMVRPK
CCCCCCCHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
LKSGQMSVHMLHLGKYWASNPYRYVDVVDGFPVPPLPDSFVDLAHRALLSAGSLSNSLQS
CCCCCEEEEEEEECHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHH
WSEAYRAEAALVNYYSPDASMGMHQDANEESEAPVISLSIGDTGIFRLGGTLNRNKPWTD
HHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCC
IPLMSGDLIVFGGANRRAFHGIPFIEANTAPAGCGLKEGRINITIRQLAL
CCEECCCEEEEECCCCCEECCCCEEECCCCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
PTLFDDPLHQLPRPPRRVAAGVVHLPNFLGLAEQKALVAQARDLAREVVGTPLAMVRPK
CCCCCCHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
LKSGQMSVHMLHLGKYWASNPYRYVDVVDGFPVPPLPDSFVDLAHRALLSAGSLSNSLQS
CCCCCEEEEEEEECHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHH
WSEAYRAEAALVNYYSPDASMGMHQDANEESEAPVISLSIGDTGIFRLGGTLNRNKPWTD
HHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCC
IPLMSGDLIVFGGANRRAFHGIPFIEANTAPAGCGLKEGRINITIRQLAL
CCEECCCEEEEECCCCCEECCCCEEECCCCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]