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The map label for this gene is kdgA [H]

Identifier: 145294251

GI number: 145294251

Start: 226353

End: 226940

Strand: Direct

Name: kdgA [H]

Synonym: cgR_0207

Alternate gene names: 145294251

Gene position: 226353-226940 (Clockwise)

Preceding gene: 145294249

Following gene: 145294252

Centisome position: 6.83

GC content: 52.72

Gene sequence:

>588_bases
TTGCTGATCATTCGTACACCGGATGCAGACAGCGCTTATCAGGTCGCACGTGCTGCTATCGAAGGTGGAATCCGCTGCTT
GGAGATCCCTTTCGGAGTCCCTGGGGCGCTTCAAGCAATTGAGAAAATTGCGACAGAGTACGGAGATCAAGGCGTATACG
TCGGAGCTGGAACAGTTCTAGACGCACAATCCGCATATGCAGCGGTCAACGCAGGATCCTCATTGCTTGTCAGCCCCAAC
GTGAATGACGGGGTCTTGGAAATCGCACAGCGATACCAAGTGGTTTCCATGCCGGGAGCATTCACCCCAACTGAAATTCA
AACTGCCCTTGAAAAAGGCGCAGATGTGGTCAAGCTTTTCCCTGCAGAGCTTCACGGACCGAAGTACCTCAAATCACTTC
GCACCCCGCTCGAGCACGCGCCAATTGCACCAACGGGTGGCGTTGATCTTGACACAGTCGGAGATTGGTTTGATGCAGGG
GCCTTCTCTGTTGGAGTTTCAAGTTATATCTGCAAGGCAGGCAGTGCCGAAGCTATTACGGCAAACGCTGCGTCCTTGCT
CCAAAAAATTGCTGATGTAAGGAGCTGA

Upstream 100 bases:

>100_bases
CATTTGGGGTGAAATGGTTGCTTTTGTGAATCTGTGTGATAGTTTGTGAGTCATGTCATTCAAATTGCACACACTTAATG
CACTTCACAGCACTGGAGCG

Downstream 100 bases:

>100_bases
ACATGAGTACTCAAGCAACCCCTAGATCGCAGAAAGCATCGTCTTCCTGGGATTTTACAACGATCAAGAAGATGCGCTGG
TTCTTCATCGCACTGATCGT

Product: hypothetical protein

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MLIIRTPDADSAYQVARAAIEGGIRCLEIPFGVPGALQAIEKIATEYGDQGVYVGAGTVLDAQSAYAAVNAGSSLLVSPN
VNDGVLEIAQRYQVVSMPGAFTPTEIQTALEKGADVVKLFPAELHGPKYLKSLRTPLEHAPIAPTGGVDLDTVGDWFDAG
AFSVGVSSYICKAGSAEAITANAASLLQKIADVRS

Sequences:

>Translated_195_residues
MLIIRTPDADSAYQVARAAIEGGIRCLEIPFGVPGALQAIEKIATEYGDQGVYVGAGTVLDAQSAYAAVNAGSSLLVSPN
VNDGVLEIAQRYQVVSMPGAFTPTEIQTALEKGADVVKLFPAELHGPKYLKSLRTPLEHAPIAPTGGVDLDTVGDWFDAG
AFSVGVSSYICKAGSAEAITANAASLLQKIADVRS
>Mature_195_residues
MLIIRTPDADSAYQVARAAIEGGIRCLEIPFGVPGALQAIEKIATEYGDQGVYVGAGTVLDAQSAYAAVNAGSSLLVSPN
VNDGVLEIAQRYQVVSMPGAFTPTEIQTALEKGADVVKLFPAELHGPKYLKSLRTPLEHAPIAPTGGVDLDTVGDWFDAG
AFSVGVSSYICKAGSAEAITANAASLLQKIADVRS

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI48994954, Length=188, Percent_Identity=35.6382978723404, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1788156, Length=187, Percent_Identity=29.9465240641711, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 20192; Mature: 20192

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIIRTPDADSAYQVARAAIEGGIRCLEIPFGVPGALQAIEKIATEYGDQGVYVGAGTVL
CEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCEE
DAQSAYAAVNAGSSLLVSPNVNDGVLEIAQRYQVVSMPGAFTPTEIQTALEKGADVVKLF
ECHHHHHHCCCCCEEEECCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHCCCCEEEEC
PAELHGPKYLKSLRTPLEHAPIAPTGGVDLDTVGDWFDAGAFSVGVSSYICKAGSAEAIT
CHHCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCCEEH
ANAASLLQKIADVRS
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIIRTPDADSAYQVARAAIEGGIRCLEIPFGVPGALQAIEKIATEYGDQGVYVGAGTVL
CEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCEE
DAQSAYAAVNAGSSLLVSPNVNDGVLEIAQRYQVVSMPGAFTPTEIQTALEKGADVVKLF
ECHHHHHHCCCCCEEEECCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHCCCCEEEEC
PAELHGPKYLKSLRTPLEHAPIAPTGGVDLDTVGDWFDAGAFSVGVSSYICKAGSAEAIT
CHHCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCCEEH
ANAASLLQKIADVRS
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]