BacMap

Definition	Corynebacterium glutamicum R chromosome, complete genome.
Accession	NC_009342
Length	3,314,179

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The map label for this gene is yafB [C]

Identifier: 145294209

GI number: 145294209

Start: 182030

End: 182380

Strand: Direct

Name: yafB [C]

Synonym: cgR_0166

Alternate gene names: 145294209

Gene position: 182030-182380 (Clockwise)

Preceding gene: 145294208

Following gene: 145294210

Centisome position: 5.49

GC content: 60.97

Gene sequence:

>351_bases
ATGACACCTCAATCAGCACCCTCCCACCCTGTCCCCACCATCGAACTCAACAACAACGTGTCCATGCCGGCCTTAGGTTT
CGGGGTGTTCCAAACCCCTCCCGCGGACACGATGACGGCCGTGACCACGGCCCTGGGCACCGGCTACCGGCATATCGACA
CCGCCGCGGCCTATGGCAACGAACGCGAGGTCGGCCAAGCCATCGCCACTTCCGGGTTGTCCCGTCAGGAGGTATTCATC
GAGACCAAGGTCTGGATCACCGACTACGGCTACGATAAGACCCTGCACGCCTTCGATAAGTCCGCCGGCAAACTCGGCGT
AGACACCATCGACCTGTTGATCCTCCACTAG

Upstream 100 bases:

>100_bases
AGAACCTGCGCCTGCTCGCCAGCTGGACGGTCACCGGTACTGATAAAACAGGCACTGACAGGGCCTTCCACCCGGCGGAC
GATGTCCCTAGTCTGGAGGA

Downstream 100 bases:

>100_bases
GCGCTGCCGGAGGACTTTGAAGCCACGATCGAGGCCTACCGGGCGTTGGAGAGACTGCAGGCCGACGGGCGGGTCCGAGC
GATTGGGGTTAGCAATTTCA

Product: hypothetical protein

Products: morphinone; NADH; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 116; Mature: 115

Protein sequence:

>116_residues
MTPQSAPSHPVPTIELNNNVSMPALGFGVFQTPPADTMTAVTTALGTGYRHIDTAAAYGNEREVGQAIATSGLSRQEVFI
ETKVWITDYGYDKTLHAFDKSAGKLGVDTIDLLILH

Sequences:

>Translated_116_residues
MTPQSAPSHPVPTIELNNNVSMPALGFGVFQTPPADTMTAVTTALGTGYRHIDTAAAYGNEREVGQAIATSGLSRQEVFI
ETKVWITDYGYDKTLHAFDKSAGKLGVDTIDLLILH
>Mature_115_residues
TPQSAPSHPVPTIELNNNVSMPALGFGVFQTPPADTMTAVTTALGTGYRHIDTAAAYGNEREVGQAIATSGLSRQEVFIE
TKVWITDYGYDKTLHAFDKSAGKLGVDTIDLLILH

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=109, Percent_Identity=33.9449541284404, Blast_Score=79, Evalue=7e-16,
Organism=Homo sapiens, GI5174391, Length=109, Percent_Identity=33.9449541284404, Blast_Score=79, Evalue=7e-16,
Organism=Homo sapiens, GI223468663, Length=107, Percent_Identity=34.5794392523364, Blast_Score=79, Evalue=1e-15,
Organism=Homo sapiens, GI24497585, Length=110, Percent_Identity=38.1818181818182, Blast_Score=77, Evalue=3e-15,
Organism=Homo sapiens, GI5453543, Length=111, Percent_Identity=32.4324324324324, Blast_Score=75, Evalue=1e-14,
Organism=Homo sapiens, GI4502049, Length=76, Percent_Identity=46.0526315789474, Blast_Score=74, Evalue=2e-14,
Organism=Homo sapiens, GI310109922, Length=111, Percent_Identity=32.4324324324324, Blast_Score=74, Evalue=2e-14,
Organism=Homo sapiens, GI310109920, Length=111, Percent_Identity=32.4324324324324, Blast_Score=74, Evalue=2e-14,
Organism=Homo sapiens, GI45446745, Length=111, Percent_Identity=32.4324324324324, Blast_Score=74, Evalue=3e-14,
Organism=Homo sapiens, GI4503285, Length=111, Percent_Identity=32.4324324324324, Blast_Score=74, Evalue=3e-14,
Organism=Homo sapiens, GI310109926, Length=111, Percent_Identity=32.4324324324324, Blast_Score=74, Evalue=3e-14,
Organism=Homo sapiens, GI207028673, Length=111, Percent_Identity=32.4324324324324, Blast_Score=74, Evalue=4e-14,
Organism=Homo sapiens, GI300116273, Length=119, Percent_Identity=35.2941176470588, Blast_Score=71, Evalue=2e-13,
Organism=Homo sapiens, GI5174695, Length=119, Percent_Identity=35.2941176470588, Blast_Score=71, Evalue=2e-13,
Organism=Homo sapiens, GI24497583, Length=111, Percent_Identity=32.4324324324324, Blast_Score=71, Evalue=2e-13,
Organism=Homo sapiens, GI300116271, Length=119, Percent_Identity=35.2941176470588, Blast_Score=71, Evalue=2e-13,
Organism=Homo sapiens, GI93277124, Length=99, Percent_Identity=35.3535353535354, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1786400, Length=97, Percent_Identity=44.3298969072165, Blast_Score=90, Evalue=3e-20,
Organism=Escherichia coli, GI87082198, Length=104, Percent_Identity=43.2692307692308, Blast_Score=89, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17550248, Length=108, Percent_Identity=39.8148148148148, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71998625, Length=106, Percent_Identity=36.7924528301887, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17566692, Length=113, Percent_Identity=36.283185840708, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17537077, Length=110, Percent_Identity=36.3636363636364, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17537075, Length=110, Percent_Identity=36.3636363636364, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17537079, Length=108, Percent_Identity=30.5555555555556, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17561298, Length=107, Percent_Identity=32.7102803738318, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324694, Length=103, Percent_Identity=45.6310679611651, Blast_Score=100, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320576, Length=104, Percent_Identity=45.1923076923077, Blast_Score=94, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322556, Length=109, Percent_Identity=33.9449541284404, Blast_Score=74, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319625, Length=117, Percent_Identity=40.1709401709402, Blast_Score=74, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321896, Length=107, Percent_Identity=32.7102803738318, Blast_Score=72, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45553081, Length=109, Percent_Identity=40.3669724770642, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24663317, Length=109, Percent_Identity=38.5321100917431, Blast_Score=82, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24662789, Length=109, Percent_Identity=37.6146788990826, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20129731, Length=109, Percent_Identity=40.3669724770642, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24657054, Length=104, Percent_Identity=37.5, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356425, Length=113, Percent_Identity=32.7433628318584, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI221378297, Length=105, Percent_Identity=36.1904761904762, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24644950, Length=105, Percent_Identity=36.1904761904762, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24662781, Length=81, Percent_Identity=38.2716049382716, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: 1.1.1.218

Molecular weight: Translated: 12434; Mature: 12303

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQSAPSHPVPTIELNNNVSMPALGFGVFQTPPADTMTAVTTALGTGYRHIDTAAAYGN
CCCCCCCCCCCCEEEECCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCC
EREVGQAIATSGLSRQEVFIETKVWITDYGYDKTLHAFDKSAGKLGVDTIDLLILH
CHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCHHHHHHCCCCCCCCCEEEEEEEC
>Mature Secondary Structure 
TPQSAPSHPVPTIELNNNVSMPALGFGVFQTPPADTMTAVTTALGTGYRHIDTAAAYGN
CCCCCCCCCCCEEEECCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCC
EREVGQAIATSGLSRQEVFIETKVWITDYGYDKTLHAFDKSAGKLGVDTIDLLILH
CHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCHHHHHHCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: morphine; NAD+; NADP+

Specific reaction: morphine + NAD(P)+ = morphinone + NAD(P)H + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA