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The map label for this gene is spoU [H]

Identifier: 138897021

GI number: 138897021

Start: 3499445

End: 3500182

Strand: Reverse

Name: spoU [H]

Synonym: GTNG_3392

Alternate gene names: 138897021

Gene position: 3500182-3499445 (Counterclockwise)

Preceding gene: 138897023

Following gene: 138897020

Centisome position: 98.59

GC content: 50.27

Gene sequence:

>738_bases
ATGAAGGTGATGATGATGGAGGAACGGGAAGCGAAAGCATTTCTTGAACAATTGCAGCAAGAAGGATTGCTCACCGAGCA
AACGCGTCTAATTTGGGAAATGATTTTGCCAAAGCGGCTAAAACGGATGTATGACGTGTTGAACGAGCGCACCCGCTATA
TCACTGTCTTGATGGAAGCGGTGGATGATCCGCACAACCAAGCCGCCGTGTTGCGAACAGCGGAGGCGTTTGGCATTCAA
GATGTCCATGTCGTCACCGGGAGGGCGCCGTTTTCGCCGAATCGGCTTGTGACGCGCTATGCCGACCAATGGCTGACGCT
TCACCGGCAACCGGATATCAAAACAGCCATCAGCGACTTAAAACAGCAAGGGTATCAAGTGTATGCGAGCTATCTCGGCG
AAGGGACGATTCCGCTTTCCGAGGTAAATGTTTCTAAGCCGACAGTGTTATTGTTCGGCAACGAACATAGCGGTGTATCG
GAAGAGGCGCTCCGTTTGGCAGATGGGACGTTCGTCATCCCGATGTACGGCTTTGTCCAAAGTTTTAACATTTCTGTTGC
TGCGGCATTGACGCTGTATGATGTCACTGAGCGGGCGCGGCGCCAAGTAGGGAGACGCTATTATTTGTCACATGAAGAGA
AAAAGACACTGTACGAACAATGGATGTGGCAGACATTGCATCCGCGCATCCGCAAACAATTGGAACAGCAAGGATATGTA
CCAACAATAGGAAAATAA

Upstream 100 bases:

>100_bases
ATATTATTTATTGAATAATCAAAATATTATTTGAAATAGGGGGAATTGCCTCCTTGCCTTATATTTTGTACAATGGGAAA
CGCAAAATAAGCGGAACGGA

Downstream 100 bases:

>100_bases
CCGGGTTCTTTTGAGAAGGGAACCCGGTTATTTTTTTTTGATGGAATATTTTTGTGTGAATACCCTTCCGGATCAATATT
TCCCTAATCGAGGAAAATGG

Product: rRNA methylase SpoU

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKVMMMEEREAKAFLEQLQQEGLLTEQTRLIWEMILPKRLKRMYDVLNERTRYITVLMEAVDDPHNQAAVLRTAEAFGIQ
DVHVVTGRAPFSPNRLVTRYADQWLTLHRQPDIKTAISDLKQQGYQVYASYLGEGTIPLSEVNVSKPTVLLFGNEHSGVS
EEALRLADGTFVIPMYGFVQSFNISVAAALTLYDVTERARRQVGRRYYLSHEEKKTLYEQWMWQTLHPRIRKQLEQQGYV
PTIGK

Sequences:

>Translated_245_residues
MKVMMMEEREAKAFLEQLQQEGLLTEQTRLIWEMILPKRLKRMYDVLNERTRYITVLMEAVDDPHNQAAVLRTAEAFGIQ
DVHVVTGRAPFSPNRLVTRYADQWLTLHRQPDIKTAISDLKQQGYQVYASYLGEGTIPLSEVNVSKPTVLLFGNEHSGVS
EEALRLADGTFVIPMYGFVQSFNISVAAALTLYDVTERARRQVGRRYYLSHEEKKTLYEQWMWQTLHPRIRKQLEQQGYV
PTIGK
>Mature_245_residues
MKVMMMEEREAKAFLEQLQQEGLLTEQTRLIWEMILPKRLKRMYDVLNERTRYITVLMEAVDDPHNQAAVLRTAEAFGIQ
DVHVVTGRAPFSPNRLVTRYADQWLTLHRQPDIKTAISDLKQQGYQVYASYLGEGTIPLSEVNVSKPTVLLFGNEHSGVS
EEALRLADGTFVIPMYGFVQSFNISVAAALTLYDVTERARRQVGRRYYLSHEEKKTLYEQWMWQTLHPRIRKQLEQQGYV
PTIGK

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=171, Percent_Identity=39.1812865497076, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 28422; Mature: 28422

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVMMMEEREAKAFLEQLQQEGLLTEQTRLIWEMILPKRLKRMYDVLNERTRYITVLMEA
CCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDDPHNQAAVLRTAEAFGIQDVHVVTGRAPFSPNRLVTRYADQWLTLHRQPDIKTAISDL
HCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
KQQGYQVYASYLGEGTIPLSEVNVSKPTVLLFGNEHSGVSEEALRLADGTFVIPMYGFVQ
HHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEHHHHHH
SFNISVAAALTLYDVTERARRQVGRRYYLSHEEKKTLYEQWMWQTLHPRIRKQLEQQGYV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCC
PTIGK
CCCCC
>Mature Secondary Structure
MKVMMMEEREAKAFLEQLQQEGLLTEQTRLIWEMILPKRLKRMYDVLNERTRYITVLMEA
CCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDDPHNQAAVLRTAEAFGIQDVHVVTGRAPFSPNRLVTRYADQWLTLHRQPDIKTAISDL
HCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
KQQGYQVYASYLGEGTIPLSEVNVSKPTVLLFGNEHSGVSEEALRLADGTFVIPMYGFVQ
HHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEHHHHHH
SFNISVAAALTLYDVTERARRQVGRRYYLSHEEKKTLYEQWMWQTLHPRIRKQLEQQGYV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCC
PTIGK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]