Click here to switch to the map view.

The map label for this gene is ndhF [H]

Identifier: 138896925

GI number: 138896925

Start: 3404319

End: 3406172

Strand: Reverse

Name: ndhF [H]

Synonym: GTNG_3294

Alternate gene names: 138896925

Gene position: 3406172-3404319 (Counterclockwise)

Preceding gene: 138896926

Following gene: 138896924

Centisome position: 95.94

GC content: 56.31

Gene sequence:

>1854_bases
ATGATGAACCTAGCTTGGGTTGTACCGCTGTTCCCGCTTTGTTCATTTGCTTTGTTGCTGTTGTTCGGACGGAAATGGAA
GAAAGCGAGCGCCTATGTTGGCATTGTTGCCACGCTTCTGTCGTTGCTTGCGGCGCTCGGTGTACTGGCCGGACGCCTCG
GCGGAACGACATATGAAGCGAACTGGACATGGATGCAGATCGGTGACGTGACGTTGACGGCCGGATTTTCCGTTACAGCG
TTAAACGCATGGATGCTCGTTGTCGTCGCTTTTGTCAGCTGGCTCGTCCATATTTATTCGCAAGGCTATATGGCCGGGGA
TGAGCGGTTTTCGACGTTTTATGCATATTTAGGCTTGTTTACGTTTGCCATGCTTGGGCTTGTGCTGTCGCCGAACTTGC
TGCAGGCGTACATCTTTTGGGAGCTCGTCGGGCTCGGGTCGTTTTTGCTGATCGGCTTTTATTTCTACAAAGAAGAAGCA
AAAGCGGCCGCGAAAAAAGCGTTTATTATGACGCGCATCGGTGACGTCGGCTTTTTTATCGGTATGTTGCTGTTGTTTTG
GCAGACGAAAAGCTTTGACTACGACGACATTTTCCGCGCCGTCGGCGACGGCACGCTGTCGCCGGGCATGACGACACTGA
CGGCCATCCTCATTTTCATCGGCGCCATCGGCAAGTCTGGGCAGTTCCCGCTTCATACGTGGCTCCCGGATGCGATGGAA
GGGCCGACGCCCGTGTCGGCGCTCATCCACGCCGCGACGATGGTGGCGGCTGGGGTGTACCTTGTGGCGGCGTTGTTCCC
GCTCTATGAGGCAAGCGAGGCTGCCATGATGACCGTGGCGGTCATCGGTGGGGTGACAGCGATTTTTGCGGCGACGATCG
GTTTGGTACAGACGGACATTAAACGTGTCCTCGCTTACTCGACGATTAGTCAGCTCGGCTATATGATGCTTGCGCTCGGC
TCGGGCAGTTATGTCGCCGCCATCTTCCATTTGACGACGCACGCCTTTTTCAAAGCGTTGCTCTTTTTGGCCGCCGGCAG
CGTCATTCACGCCGTCCATACGCAAAACATTGAAGAAATGGGCGGGCTTTGGCGCCGGCTGCCGTGGACTGCGCCGCTCT
TTCTTGTCGGGGCGCTATCGATTAGTGGAGTGCCGCTGTTTGCCGGCTTTTTCAGCAAGGATGAAATTTTAGCGGCCGCT
TGGTTGAACGGATCGGTTGAGCTCTTTTGGCTGGCGGTAGCGGCGGCGCTGTTGACATCATTTTATATGTTCCGGCTCTT
TTTCCTCGTCTTTACTGGTGAGGCGCGCCATGATGGAGATGCTCATGAAGCACCGATGTCGATGGTCGGGCCAATGGTCG
TTCTCGGCGTATTATCAGTCGCTTCGGGATACATTCAAACCCCTTGGTTTGGATCGTTTCTCGGCGAGTGGCTGACCGAC
GGGGCGTACCATCATGAAGCAGCGCCGGGATGGATCGCTGTTGTGGCGACGATTGTGTCGCTTGTGGGGATTCTCCTTGC
CTGGCTGATGTACGGGAAGCGGACTGTGCCGCGCGACTGGTTATCCGCGCGTGCACCGCACGGGTACGAATTGTTGCGCC
GTCACTATTACGTTGATGACTTTTACCGGTGGACCGTTGTGGCGCTTGTGTCATTCATTAGCCGCCTGTTGGCATATGTC
GACCGTTACCTTGTGGAAGGGATCGTTCGGCTTGTCACCGGCACGGTCAGTGCCGCTGCTTCCGCCGGATCGCGCGGGCA
AAACGGGCAAGCGCAGACGTACGGGGCGGTGACGGTGGCTGGCTTGGCCATTTTGGTCGTCATCTTCGCGTTGACAGGGG
GGTACTGGCTATGA

Upstream 100 bases:

>100_bases
TGTCGGATTGGCAGCGCTCATCGCCTTTTACCGCAACCGCAAAACGGTTCAAGTCGATGAAGCCAATTCATTGAAACATT
AGAAAAGGGGATGGGGAAAG

Downstream 100 bases:

>100_bases
GCTTTTTGTCGCTGCTCGTCTTTTCGCCATTGTTAGGTATCATTTTGCTGGCGTTTGTCCGCCGAACGGATACGAAAACG
ATTCAATGGCTCGGGACGGC

Product: NADH dehydrogenase subunit L

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MMNLAWVVPLFPLCSFALLLLFGRKWKKASAYVGIVATLLSLLAALGVLAGRLGGTTYEANWTWMQIGDVTLTAGFSVTA
LNAWMLVVVAFVSWLVHIYSQGYMAGDERFSTFYAYLGLFTFAMLGLVLSPNLLQAYIFWELVGLGSFLLIGFYFYKEEA
KAAAKKAFIMTRIGDVGFFIGMLLLFWQTKSFDYDDIFRAVGDGTLSPGMTTLTAILIFIGAIGKSGQFPLHTWLPDAME
GPTPVSALIHAATMVAAGVYLVAALFPLYEASEAAMMTVAVIGGVTAIFAATIGLVQTDIKRVLAYSTISQLGYMMLALG
SGSYVAAIFHLTTHAFFKALLFLAAGSVIHAVHTQNIEEMGGLWRRLPWTAPLFLVGALSISGVPLFAGFFSKDEILAAA
WLNGSVELFWLAVAAALLTSFYMFRLFFLVFTGEARHDGDAHEAPMSMVGPMVVLGVLSVASGYIQTPWFGSFLGEWLTD
GAYHHEAAPGWIAVVATIVSLVGILLAWLMYGKRTVPRDWLSARAPHGYELLRRHYYVDDFYRWTVVALVSFISRLLAYV
DRYLVEGIVRLVTGTVSAAASAGSRGQNGQAQTYGAVTVAGLAILVVIFALTGGYWL

Sequences:

>Translated_617_residues
MMNLAWVVPLFPLCSFALLLLFGRKWKKASAYVGIVATLLSLLAALGVLAGRLGGTTYEANWTWMQIGDVTLTAGFSVTA
LNAWMLVVVAFVSWLVHIYSQGYMAGDERFSTFYAYLGLFTFAMLGLVLSPNLLQAYIFWELVGLGSFLLIGFYFYKEEA
KAAAKKAFIMTRIGDVGFFIGMLLLFWQTKSFDYDDIFRAVGDGTLSPGMTTLTAILIFIGAIGKSGQFPLHTWLPDAME
GPTPVSALIHAATMVAAGVYLVAALFPLYEASEAAMMTVAVIGGVTAIFAATIGLVQTDIKRVLAYSTISQLGYMMLALG
SGSYVAAIFHLTTHAFFKALLFLAAGSVIHAVHTQNIEEMGGLWRRLPWTAPLFLVGALSISGVPLFAGFFSKDEILAAA
WLNGSVELFWLAVAAALLTSFYMFRLFFLVFTGEARHDGDAHEAPMSMVGPMVVLGVLSVASGYIQTPWFGSFLGEWLTD
GAYHHEAAPGWIAVVATIVSLVGILLAWLMYGKRTVPRDWLSARAPHGYELLRRHYYVDDFYRWTVVALVSFISRLLAYV
DRYLVEGIVRLVTGTVSAAASAGSRGQNGQAQTYGAVTVAGLAILVVIFALTGGYWL
>Mature_617_residues
MMNLAWVVPLFPLCSFALLLLFGRKWKKASAYVGIVATLLSLLAALGVLAGRLGGTTYEANWTWMQIGDVTLTAGFSVTA
LNAWMLVVVAFVSWLVHIYSQGYMAGDERFSTFYAYLGLFTFAMLGLVLSPNLLQAYIFWELVGLGSFLLIGFYFYKEEA
KAAAKKAFIMTRIGDVGFFIGMLLLFWQTKSFDYDDIFRAVGDGTLSPGMTTLTAILIFIGAIGKSGQFPLHTWLPDAME
GPTPVSALIHAATMVAAGVYLVAALFPLYEASEAAMMTVAVIGGVTAIFAATIGLVQTDIKRVLAYSTISQLGYMMLALG
SGSYVAAIFHLTTHAFFKALLFLAAGSVIHAVHTQNIEEMGGLWRRLPWTAPLFLVGALSISGVPLFAGFFSKDEILAAA
WLNGSVELFWLAVAAALLTSFYMFRLFFLVFTGEARHDGDAHEAPMSMVGPMVVLGVLSVASGYIQTPWFGSFLGEWLTD
GAYHHEAAPGWIAVVATIVSLVGILLAWLMYGKRTVPRDWLSARAPHGYELLRRHYYVDDFYRWTVVALVSFISRLLAYV
DRYLVEGIVRLVTGTVSAAASAGSRGQNGQAQTYGAVTVAGLAILVVIFALTGGYWL

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=454, Percent_Identity=35.4625550660793, Blast_Score=269, Evalue=6e-72,
Organism=Escherichia coli, GI1788614, Length=526, Percent_Identity=44.106463878327, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI1788829, Length=496, Percent_Identity=35.4838709677419, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI1788827, Length=429, Percent_Identity=30.3030303030303, Blast_Score=155, Evalue=9e-39,
Organism=Escherichia coli, GI1788831, Length=338, Percent_Identity=31.0650887573964, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI1788613, Length=289, Percent_Identity=29.0657439446367, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI145693160, Length=325, Percent_Identity=28.6153846153846, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI2367154, Length=217, Percent_Identity=29.0322580645161, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 67046; Mature: 67046

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNLAWVVPLFPLCSFALLLLFGRKWKKASAYVGIVATLLSLLAALGVLAGRLGGTTYEA
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
NWTWMQIGDVTLTAGFSVTALNAWMLVVVAFVSWLVHIYSQGYMAGDERFSTFYAYLGLF
CEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TFAMLGLVLSPNLLQAYIFWELVGLGSFLLIGFYFYKEEAKAAAKKAFIMTRIGDVGFFI
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMLLLFWQTKSFDYDDIFRAVGDGTLSPGMTTLTAILIFIGAIGKSGQFPLHTWLPDAME
HHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCC
GPTPVSALIHAATMVAAGVYLVAALFPLYEASEAAMMTVAVIGGVTAIFAATIGLVQTDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRVLAYSTISQLGYMMLALGSGSYVAAIFHLTTHAFFKALLFLAAGSVIHAVHTQNIEEM
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
GGLWRRLPWTAPLFLVGALSISGVPLFAGFFSKDEILAAAWLNGSVELFWLAVAAALLTS
HHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHH
FYMFRLFFLVFTGEARHDGDAHEAPMSMVGPMVVLGVLSVASGYIQTPWFGSFLGEWLTD
HHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
GAYHHEAAPGWIAVVATIVSLVGILLAWLMYGKRTVPRDWLSARAPHGYELLRRHYYVDD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHH
FYRWTVVALVSFISRLLAYVDRYLVEGIVRLVTGTVSAAASAGSRGQNGQAQTYGAVTVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
GLAILVVIFALTGGYWL
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMNLAWVVPLFPLCSFALLLLFGRKWKKASAYVGIVATLLSLLAALGVLAGRLGGTTYEA
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
NWTWMQIGDVTLTAGFSVTALNAWMLVVVAFVSWLVHIYSQGYMAGDERFSTFYAYLGLF
CEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TFAMLGLVLSPNLLQAYIFWELVGLGSFLLIGFYFYKEEAKAAAKKAFIMTRIGDVGFFI
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMLLLFWQTKSFDYDDIFRAVGDGTLSPGMTTLTAILIFIGAIGKSGQFPLHTWLPDAME
HHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCC
GPTPVSALIHAATMVAAGVYLVAALFPLYEASEAAMMTVAVIGGVTAIFAATIGLVQTDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRVLAYSTISQLGYMMLALGSGSYVAAIFHLTTHAFFKALLFLAAGSVIHAVHTQNIEEM
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
GGLWRRLPWTAPLFLVGALSISGVPLFAGFFSKDEILAAAWLNGSVELFWLAVAAALLTS
HHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHH
FYMFRLFFLVFTGEARHDGDAHEAPMSMVGPMVVLGVLSVASGYIQTPWFGSFLGEWLTD
HHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
GAYHHEAAPGWIAVVATIVSLVGILLAWLMYGKRTVPRDWLSARAPHGYELLRRHYYVDD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHH
FYRWTVVALVSFISRLLAYVDRYLVEGIVRLVTGTVSAAASAGSRGQNGQAQTYGAVTVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
GLAILVVIFALTGGYWL
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]