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The map label for this gene is gsiB [H]

Identifier: 138896914

GI number: 138896914

Start: 3391706

End: 3393265

Strand: Reverse

Name: gsiB [H]

Synonym: GTNG_3283

Alternate gene names: 138896914

Gene position: 3393265-3391706 (Counterclockwise)

Preceding gene: 138896915

Following gene: 138896913

Centisome position: 95.58

GC content: 44.17

Gene sequence:

>1560_bases
TTGAAAAAGAAGTATTGGTCTTACCTGTTGTCTGCTCTCGTACTCGCTGTTTCTACGGCATTGGCCGGATGCGGCGGCAA
ATCGGCTTCCAATCACGCATCATCCGGTTCACAAGGGAAAGGAAACGTTGCTCAGGAGCTGACATATGCGACGACGTCTG
ATGTGGTTGGCCTTTCACCAACGTTAACGAACGATTCCGTATCATCGAAGGTAATCGATCAAGTGTATGAAACGTTATTT
GTTCGCGATCCGAAAACGATGGAAATCAAACCTCATTTAGCGGAATCGTACGAAAATCCGAATGATAAAACATGGGTTAT
CAAATTGAAACAAGGCATTAAGTTCCACGATGGTACCGATTTTAACGCCGAAGCAGTGAAATATACGTTTGATAAATTAA
GAGATCCGAAAACAGCAGCACCGCGTGCTTCATTGCTTGAACCGGTTGAATCGGTGGAAGTGAAAGACGATTATACTGTT
GTGATCACAACGAAATATCCTTATGGTCCGATGTTATCTGCCTTAGCACATTCGAACGCTGCAATCATCAGCCCGACGGC
CGATCAAAAACAAGATTTAATGAAATATCCAGTTGGTACAGGTCCGTTTAAATTCGTCGAATGGGTGCCAGGTGATCATG
TGACGCTAGAGAAAAACGACGACTATTGGCAAGGGGCGCCGAAATTAGAGAAAGTCACATTCAAAGTCGTTCCGGAAGTG
ACGACTGCCATTTCAATGTTGCAAACTGGCGATGTGCAGTTTATCGACAATATTCCGGCGGAACAACTTTCCCGTATTGA
AGCGATGAAAAATGTTCAGTTCATCAAAAAAGAAGGCACACCGGTCTACTATCTAGGTTTTAATATGAAAAAGAAACCGA
TGAATGAACTTGCATTCCGCCAAGCCGTTTCGTATGCGATCAACAAGGATGAATACATCCAACAGTTGAAAGGTCTCGGC
GTGAAATCAAATAGCGTCATCGGCCCGAAAGTGTTCGGCTATGATGAGTCGTCAGAAAACGTCGCTTACAGCTACGATCC
GCAAAAAGCAAAACAGCTTATCGAAGAACAGGGCTATAAAGGGACAACCGTTAAAATGCTTGTTGCTAATACAGCTGCCC
ATAGGAAAATGGCGGAGATCGTCCAAGCTCAGTTGGAGGAAGTTGGTATTCACACTGAAATTGAGTCGATGGAGTGGGGG
ACATTCTTAGATTCAGCGAGACAAGGGAAATATGATATCACATTCTTGAGTTGGGCGAACCTTACTGCCGACGGCAGCGA
GTTGTTTTACCCGAACTTCCATTCCAAAAATGCAGGGGCAACAAACCGTATATTCTACAACAACCCGACGTTTGACAAAC
TTGTTGAAGAATCGAGAAATGCGATTGATCCAGAAGTGCGCAAACAAAAATTAAAAGAAGCAAACGAATTTTTGTTGAAA
GACGCGGCTGTTGTCGTAATGAATCACGGCGTTGTCACCGCGGTCGTTGATCAATCAGTGAAAGGATTAGAGATCGATCC
AACAGGACAATGGTCACTCTATCACGTTCATAGAGAGTAG

Upstream 100 bases:

>100_bases
TGTCGTCTTTGCTTTTAACGTCGTTGGCGACGGGCTACGCGATGCGCTTGACCCAAATATGGATATCAATCAATAAACAT
ACCATTTGGAGGGAAAGCGA

Downstream 100 bases:

>100_bases
GGGATAGCATGACTGAAACCTTGCTGCGTGTCGAGAACCTTGTTACGACCTTCAGCACAGCGGAAGGGAAACTTTCTGCT
GTGCGGGGGGTTTCGTTTTC

Product: solute-binding family 5 protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MKKKYWSYLLSALVLAVSTALAGCGGKSASNHASSGSQGKGNVAQELTYATTSDVVGLSPTLTNDSVSSKVIDQVYETLF
VRDPKTMEIKPHLAESYENPNDKTWVIKLKQGIKFHDGTDFNAEAVKYTFDKLRDPKTAAPRASLLEPVESVEVKDDYTV
VITTKYPYGPMLSALAHSNAAIISPTADQKQDLMKYPVGTGPFKFVEWVPGDHVTLEKNDDYWQGAPKLEKVTFKVVPEV
TTAISMLQTGDVQFIDNIPAEQLSRIEAMKNVQFIKKEGTPVYYLGFNMKKKPMNELAFRQAVSYAINKDEYIQQLKGLG
VKSNSVIGPKVFGYDESSENVAYSYDPQKAKQLIEEQGYKGTTVKMLVANTAAHRKMAEIVQAQLEEVGIHTEIESMEWG
TFLDSARQGKYDITFLSWANLTADGSELFYPNFHSKNAGATNRIFYNNPTFDKLVEESRNAIDPEVRKQKLKEANEFLLK
DAAVVVMNHGVVTAVVDQSVKGLEIDPTGQWSLYHVHRE

Sequences:

>Translated_519_residues
MKKKYWSYLLSALVLAVSTALAGCGGKSASNHASSGSQGKGNVAQELTYATTSDVVGLSPTLTNDSVSSKVIDQVYETLF
VRDPKTMEIKPHLAESYENPNDKTWVIKLKQGIKFHDGTDFNAEAVKYTFDKLRDPKTAAPRASLLEPVESVEVKDDYTV
VITTKYPYGPMLSALAHSNAAIISPTADQKQDLMKYPVGTGPFKFVEWVPGDHVTLEKNDDYWQGAPKLEKVTFKVVPEV
TTAISMLQTGDVQFIDNIPAEQLSRIEAMKNVQFIKKEGTPVYYLGFNMKKKPMNELAFRQAVSYAINKDEYIQQLKGLG
VKSNSVIGPKVFGYDESSENVAYSYDPQKAKQLIEEQGYKGTTVKMLVANTAAHRKMAEIVQAQLEEVGIHTEIESMEWG
TFLDSARQGKYDITFLSWANLTADGSELFYPNFHSKNAGATNRIFYNNPTFDKLVEESRNAIDPEVRKQKLKEANEFLLK
DAAVVVMNHGVVTAVVDQSVKGLEIDPTGQWSLYHVHRE
>Mature_519_residues
MKKKYWSYLLSALVLAVSTALAGCGGKSASNHASSGSQGKGNVAQELTYATTSDVVGLSPTLTNDSVSSKVIDQVYETLF
VRDPKTMEIKPHLAESYENPNDKTWVIKLKQGIKFHDGTDFNAEAVKYTFDKLRDPKTAAPRASLLEPVESVEVKDDYTV
VITTKYPYGPMLSALAHSNAAIISPTADQKQDLMKYPVGTGPFKFVEWVPGDHVTLEKNDDYWQGAPKLEKVTFKVVPEV
TTAISMLQTGDVQFIDNIPAEQLSRIEAMKNVQFIKKEGTPVYYLGFNMKKKPMNELAFRQAVSYAINKDEYIQQLKGLG
VKSNSVIGPKVFGYDESSENVAYSYDPQKAKQLIEEQGYKGTTVKMLVANTAAHRKMAEIVQAQLEEVGIHTEIESMEWG
TFLDSARQGKYDITFLSWANLTADGSELFYPNFHSKNAGATNRIFYNNPTFDKLVEESRNAIDPEVRKQKLKEANEFLLK
DAAVVVMNHGVVTAVVDQSVKGLEIDPTGQWSLYHVHRE

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=482, Percent_Identity=32.1576763485477, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1789966, Length=511, Percent_Identity=27.7886497064579, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1787762, Length=480, Percent_Identity=27.5, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1787551, Length=552, Percent_Identity=25.5434782608696, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1787495, Length=498, Percent_Identity=25.1004016064257, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1789887, Length=401, Percent_Identity=28.1795511221945, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI87081878, Length=502, Percent_Identity=22.5099601593625, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789397, Length=506, Percent_Identity=21.9367588932806, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57788; Mature: 57788

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKYWSYLLSALVLAVSTALAGCGGKSASNHASSGSQGKGNVAQELTYATTSDVVGLSP
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
TLTNDSVSSKVIDQVYETLFVRDPKTMEIKPHLAESYENPNDKTWVIKLKQGIKFHDGTD
CCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHCCCCCCCEEEEEEECCCEECCCCC
FNAEAVKYTFDKLRDPKTAAPRASLLEPVESVEVKDDYTVVITTKYPYGPMLSALAHSNA
CCHHHHHHHHHHCCCCCCCCCHHHHHCHHHHCEECCCEEEEEEECCCCCHHHHHHHCCCC
AIISPTADQKQDLMKYPVGTGPFKFVEWVPGDHVTLEKNDDYWQGAPKLEKVTFKVVPEV
EEECCCCHHHHHHHHCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCHHHEEEEECHHH
TTAISMLQTGDVQFIDNIPAEQLSRIEAMKNVQFIKKEGTPVYYLGFNMKKKPMNELAFR
HHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
QAVSYAINKDEYIQQLKGLGVKSNSVIGPKVFGYDESSENVAYSYDPQKAKQLIEEQGYK
HHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCEEEEECHHHHHHHHHHCCCC
GTTVKMLVANTAAHRKMAEIVQAQLEEVGIHTEIESMEWGTFLDSARQGKYDITFLSWAN
CCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHCCCCEEEEEEEECC
LTADGSELFYPNFHSKNAGATNRIFYNNPTFDKLVEESRNAIDPEVRKQKLKEANEFLLK
CCCCCCEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
DAAVVVMNHGVVTAVVDQSVKGLEIDPTGQWSLYHVHRE
CCEEEEEECCEEEEEECCCCCCCEECCCCCEEEEEEECC
>Mature Secondary Structure
MKKKYWSYLLSALVLAVSTALAGCGGKSASNHASSGSQGKGNVAQELTYATTSDVVGLSP
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
TLTNDSVSSKVIDQVYETLFVRDPKTMEIKPHLAESYENPNDKTWVIKLKQGIKFHDGTD
CCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHCCCCCCCEEEEEEECCCEECCCCC
FNAEAVKYTFDKLRDPKTAAPRASLLEPVESVEVKDDYTVVITTKYPYGPMLSALAHSNA
CCHHHHHHHHHHCCCCCCCCCHHHHHCHHHHCEECCCEEEEEEECCCCCHHHHHHHCCCC
AIISPTADQKQDLMKYPVGTGPFKFVEWVPGDHVTLEKNDDYWQGAPKLEKVTFKVVPEV
EEECCCCHHHHHHHHCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCHHHEEEEECHHH
TTAISMLQTGDVQFIDNIPAEQLSRIEAMKNVQFIKKEGTPVYYLGFNMKKKPMNELAFR
HHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
QAVSYAINKDEYIQQLKGLGVKSNSVIGPKVFGYDESSENVAYSYDPQKAKQLIEEQGYK
HHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCEEEEECHHHHHHHHHHCCCC
GTTVKMLVANTAAHRKMAEIVQAQLEEVGIHTEIESMEWGTFLDSARQGKYDITFLSWAN
CCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHCCCCEEEEEEEECC
LTADGSELFYPNFHSKNAGATNRIFYNNPTFDKLVEESRNAIDPEVRKQKLKEANEFLLK
CCCCCCEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
DAAVVVMNHGVVTAVVDQSVKGLEIDPTGQWSLYHVHRE
CCEEEEEECCEEEEEECCCCCCCEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA